附件
Summary
During the course, BASIC PLANT WATER RELATIONS and the BACKGROUND OF
THE USE OF PRESSURE PROBES will be taught in lectures during the morning. This will
include CELL PRESSURE PROBES for both single isolated cells and higher plant tissue
cells as well as ROOT PRESSURE PROBES for measuring the water uptake by roots. For the
rest of the day, the theoretical knowledge will be used in experiments. During the
experiments, we will focus on the role of AQUAPORINS (WATER CHANNELS). The
CHARA system and root cortical cells will be used to introduce basic parameters such as
hydraulic conductivity, permeability and reflection coefficients, and to study the permeability
and selectivity of aquaporins.
At the ROOT LEVEL, we will see how membrane permeability affects the overall water
transport across roots. The cell pressure probe for tissue cells will be combined with the root
pressure probe. Models for the QUANTITATIVE DESCRITPTION OF WATER FLOW IN
PLANT SYSTEMS will be discussed. Participants will calculate basic water relations
parameters from their experiments. This includes a critical examination of errors, which may
be involved with the measurements as compared with other techniques. The role of
UNSTIRRED LAYERS during measurements of water and solute permeability is another
topic. At the cell level, we will concentrate on the selectivity of aquaporins and on the
mechanisms by which water channel activity is GATED in plants. Effects of heavy metal,
osmotic, and oxidative stresses (ROS) on the activity of aquaporins will be demonstrated.
Course on Pressure Probe Techniques and Basic Water Relations
March 03 till March 14
Prof. Ernst Steudle, Dr. Qing Ye, Burkhard Stumpf & Yangmin Kim
Dept. of Plant Ecology, University of Bayreuth, Germany
Local Organiser: Prof. Weifu Lin & Mr. Feng An
Course location: Seminar room of Rubber Research Institute (RRI) of the Chinese
Academy of Tropical Agriculture Sciences (CATAS), Hainan, China;
DAY
03.03.
(Monday)
TIME
SPEAKER
8:30-10:30
Prof. E. Steudle
10:30-12:00
12:00-14:30
Teachers
TYPE OF ACTIVITY
Brief introduction into pressure probes
(PPs)
Pressure probe techniques-practicals
Lunch time & nap
04.03.
(Tuesday)
05.03.
(Wednesday)
06.03.
(Thursday)
07.03.
(Friday)
14:30-17:30
Teachers
8:30-10:30
Prof. E. Steudle
10:30-12:00
12:00-14:30
14:30-17:30
8:30-10:30
10:30-12:00
12:00-14:30
14:30-17:30
8:30-10:30
10:30-12:00
12:00-14:30
14:30-17:30
8:30-10:30
10:30-12:00
12:00-14:30
14:30-17:30
Teachers
8:30-10:30
Prof. E. Steudle
10:30-12:00
12:00-14:30
14:30-17:30
Teachers
8:30-10:30
Dr. Qing Ye
10:30-12:00
12:00-14:30
14:30-17:30
Teachers
8:30-10:30
Yangmin Kim
10:30-12:00
12:00-14:30
14:30-17:30
Teachers
8:30-10:30
Prof. JJ Zhu
10:30-12:00
Teachers
Teachers
Prof. E. Steudle
Teachers
Teachers
Prof. E. Steudle
Teachers
Teachers
Prof. E. Steudle
Teachers
Teachers
Pressure probe techniques-practicals
Basic water relations and background of
PPing
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Role of aquaporins in plant water relations
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Root pressure probe technique
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Root hydraulics (continued)
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
08.03.
(Saturday)
09.03.
(Sunday)
10.03.
(Monday)
11.03.
(Tuesday)
12.03.
(Wednesday)
13.03.
(Thursday)
Teachers
Teachers
Teachers
Cohesion-Tension theory and long distance
transport in the xylem
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Talk title: Gating of aquaporins in plants by
diffrent stresses
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Talk title: Regulation of water permeability
of a corn leaf at cell level by light: gating of
aquaporins
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Osmotic potential of cell sap:
microsampling and measuring
Pressure probe techniques-practicals
14.03.
(Friday)
12:00-14:30
14:30-17:30
Teachers
8:30-10:30
Students
10:30-12:00 Teachers
12:00-14:30
14:30-17:30 Teachers
NOTE：Time schedule is subject to change
Lunch time & nap
Pressure probe techniques-practicals
Discussions continued and Tests: Chinese
translation is available.
Pressure probe techniques-practicals
Lunch time & nap
Pressure probe techniques-practicals
Curriculum VitaeProf.
Dr. Ernst Steudle
Lehrstuhl Pflanzenökologie, Universität Bayreuth,
D-95440 Bayreuth, Germany, Tel.: (49)-921-55-2578; Fax.: (49)-921-55-2564E-
mail: [email protected]:
http://www.homepage.steudle.uni-bayreuth.de
Academic career:
1969: Diplom (M.Sc.) in chemistry; Freie Universität Berlin, Germany
Feb. 1972: Ph.D.; Technische Hochschule Aachen, Germany
1969-1984: Membrane Research Group at the Nuclear Research Center in Jülich,
Germany; Head: Prof. Dr. U. Zimmermann.
1977: Postdoc at the University of Toronto, Canada (Prof. J. Dainty).
1982: Postdoc at the University of Illinois in Urbana, USA (Prof. J.S. Boyer).
1984-1986 Head of Membrane Research Group in Jülich, Germany.
1986: Habilitation at the Universität Würzburg, Germany.
1986-present Professor Botany/Plant Biophysics at the Department of Plant Ecology,
University of Bayreuth, Germany.
Fields of Research: Plant biophysics; plant water relations; transport in plants;
biophysics of plant growth; water and nutrient transport in roots; molecular
mechanisms of water transport (aquaporins); long distance transport of water;
development of water relations technology: use of “Bayreuth pressure probes“ in
basic and agricultural research.
Visiting guest professors to laboratory (selection since 1992):
(1989) 1992, 1997, 1999, 2004: Carol A. Peterson, University of Waterloo, Ontario,
Canada
1993/4: S.W. Hallgren, Oklahoma State Univ., USA
1997/8: M.T. Tyree, University of Vermont, USA (Humboldt professor).
1996: T. Hirasawa, University of Agriculture & Technology, Tokyo, Japan
1997, 1998, 1999, 2002, etc.: David Clarkson, Long Ashton Research Station &
University of Bristol, UK
2004/5: Sui-Qui Zhang and Deng Xi-Ping, Institute of soil and water conservation
(ISWC), Chinese Academy of Sciences, Yangling, Shaanxi Prov., China.
Editorial and Review Boards:
Referee in plant transport, water relations, and general plant biophysics of the
German Science Foundation (DFG), NSF, USDA, BBSRC, and other institutions.
Editor for:
Journal of Experimental Botany (since 1994), Planta (Jan. 1994 – Aug 2005)Plant
Physiology (2000 -2005).
Advisory Boards of: Plant and Soil, Plant Biology, Environment Control in Biology
Refereeing for: Plant Physiology, Planta, Nature, Science, Plant Soil, J. Exp. Bot.,
PlantCell and Environment, Oecologia, Physiol. Plant., New Phytologist & others.
Memberships
German Botanical Society (Deutsche Botanische Gesellschaft)
Federation of European Societies of Plant Physology (FESPP)
American Society of Plant Biologists,
the former ASPP (ASPB) Society for Experimental Biology (UK).
Bayreuth, October 2007 (Prof. Dr. E. Steudle)
Appendix I List of 12 most relevant publications: 1998 -2007
(112) Steudle E, Peterson CA (1998) How does water get through roots? J. Exp. Bot.
49: 775-788
(115) Tyerman SD, Bohnert HJ, Maurel C, Steudle E, Smith JAC (1999) Plant
aquaporins: their molecular biology, biophysics and significance for plant water
relations. J. Exp. Bot. 50: 1055-1071.
(116) Wei C, Steudle E, Tyree MT (1999) Water ascent in plants: do ongoing
controversies have a sound basis? Trends Plant Science 4: 372 – 375.
(128) Steudle E (2001) The cohesion-tension mechanism and the acquisition of
water by plant roots. Annu. Rev. Plant Physiol. & Plant Mol. Biol. 52: 847-875.
(130) Hose E, Clarkson DT, Steudle E, Schreiber L, Hartung W (2001) The
exodermis: a variable apoplastic barrier. J. Exp. Bot. 52: 2245-2264.
(136) Wan XC, Steudle E, Hartung W (2004) Gating of water channels (aquaporins)
in cortical cells of young corn roots by mechanical stimuli (pressure pulses):
effects of ABA and HgCl2. J. Exp. Bot. 55: 411-422.
(140) Henzler T, Ye Q, Steudle E (2004) Oxidative gating of water channels
(aquaporins) in Chara by hydroxyl radicals. Plant Cell Environment 27: 11841195.
(142) Ranathunge K, Steudle E, Lafitte R (2005) Blockage of apoplastic bypass-flow
of water in rice roots by insoluble salt precipitates analogous to a Pfeffer cell.
Plant Cell Environment 28: 121-133.
(143) Ye Q, Muhr J, Steudle E (2005) A cohesion/tension model for the gating of
aquaporins allows estimation of water channel pore volumes in Chara. Plant,
Cell & Environment 28: 525-535.
(150) Ye Q, Steudle E (2006) Oxidative gating of water channels (aquaporins) in
corn roots. Plant, Cell & Environment 29: 459-470.
(149) Ye Q, Kim YM, Steudle E (2006) A re-examination of the minor role of
unstirred layers during the measurement of transport coefficients of Chara
corallina internodes with the cell pressure probe. Plant Cell Environment 29:
964 – 980.
(154) Knipfer T, Das D, Steudle E (2007) During measurements of root hydraulics
with pressure probes, the contribution of unstirred layers is minimized in the
pressure relaxation mode: comparison with pressure-clamp and high-pressure
flowmeter. Plant Cell Environment 30: 845-860.
Appendix II
(A) PhD students in past years:
(a) Finished PhDs:
(i)
Tobias Henzler: “Die Funktion von Wasserkanälen in pflanzlichen
Zellmembranen und ihre Bedeutung für den Wassertransport in Wurzeln“ )
(“The functioning of water channels in plant cell membranes, and its
significance for water transport in roots“; 2001.
(ii)
Hilde M. Zimmermann: “Die Rolle apolastischer Transportbarrieren
bei der radialen Wasser-und Ionenaufnahme in Maiswurzeln (Zea mays
L.)“ (“The role of apoplastic transport barriers during the radial uptake of water
and nutrient ions in corn roots (Zea mays L.)“; June 2001
(iii)
Kosala Ranathunge:“The role of apoplastic transport barriers for
radial water and ion uptake in rice (Oryza sativa L.) roots“; June 2005.
(iv)
Qing Ye:“Gating of water channels (aquaporins) in plants: effects
of osmotic and oxidative stresses and the role of unstirred layers“; November
2005.
(b) Ongoing PhDs:
(i)
Lukasz Kotula: “Regulation of oxygen and water uptake by rice
roots.“
(ii)
Yangmin Kim: “Regulation aquaporins by transpiration and light:
effect on long distance transport of water. “
(c)
PhD students from outside recently working in the lab (de facto
“sandwiched PhDs“):
(i)
Ewa Przedpelska:“Influence of heavy metals on aquaporin activity
of plant cells“. Dept. of Ecotoxicology, Warsaw University, Poland.
(ii)
Chris John Meyer: “Role of multi-layered exodermis during water
and nutrient uptake of plant roots“. School of Biology, University of Waterloo,
ON, Canada.
(B) Research performance:
Development of technology & additional teaching activities
The lab has established new technologies in plant water relations, which have
been exported in the past to other institutions all over the world. The use of
new technologies has lead to the discovery of basic mechanisms of the
regulation of plant water at the cell (membrane), tissue/organ, and whole plant
levels, which are important for both the understanding of basic water relations
and applications in agriculture. The lab has been also involved in the recent
controversy about the validity of the cohesion/tension mechanism.
International summer schools in basic water relations and use of BT
technology are usually provided (Pressure Probe Courses, PPC); BT probes
have been transferred to labs all over the world (e.g. to UK, France, Belgium,
Poland, Ireland, Spain, Russia, Australia, Korea, China & Canada). Regularly,
postdocs, PhD students, and others graduates are sent from different labs to
learn the BT technology. Sometimes, these students later became PhD
students in the lab. After the discovery of water channels (aquaporins; see
Nobel prize to P. Agre in chemistry in 2003), the popularity of BT technology
increased, both in basic and applied areas. In this context, there is long-lasting
cooperation with the International Rice Research Institute (IRRI) at Los Banos,
Manila, Philippines (see references 131, 134, 135, 142 & 148).
PPCs outside Bayreuth:
August 1993: PPC at the University of Western Australia at
Perth. February 2003: International PPC, again in
Perth/Australia July/August 2003: Int. PPC in
Gwangju/South Korea August/September 2004: Int. PPCs
in Katowice/Poland and at
China Agricultural University, Beijing/China. September/October 2005: CAS
at Yangling, Prov. Shaanxi, China September 2006: CAS/Yangling, Prov.
Shaanxi, China. September 03 till 14, 2007: Université Catholique de Louvainla-Neuve, Belgium.
Other recent invited teaching on “Biophysics of Plant Water”:
(i)
University of Silesia at Katowice/Poland within the
ERASMUS/SOCRATES PROGRAMME of EU in March/April 2002 & 2003 (8
lectures/stay).
(ii)
Lecturing at the State Key Lab of Chinese Academy of Scinces at
Yangling as a Visiting Professor, starting in 2005.
List of recent publications: 1998 -2007
(112) Steudle E, Peterson CA (1998) How does water get through roots? J. Exp. Bot. 49: 775788
(113) Zimmermann HM, Steudle E (1998) Apoplastic transport across young maize roots: effect
of the exodermis. Planta 206: 7-19
(114) Freundl E, Steudle E, Hartung W (1998) Water uptake by roots of maize and sunflower
affects the radial transport of abscisic acid and the ABA concentration in the xylem. Planta
207: 8-19
(115) Tyerman SD, Bohnert HJ, Maurel C, Steudle E, Smith JAC (1999) Plant aquaporins: their
molecular biology, biophysics and significance for plant water relations. J. Exp. Bot. 50:
1055-1071.
(116) Wei C, Steudle E, Tyree MT (1999) Water ascent in plants: do ongoing controversies have
a sound basis? Trends Plant Science 4: 372 – 375.
(117) Wei C, Tyree MT, Steudle E (1999) Direct measurement of xylem pressure in leaves of
intact maize plants. A test of cohesion-tension theory taking hydraulic archirecture into
consideration. Plant Physiol. 121: 1191 – 1205.
(118) Henzler T, Waterhouse RN, Smyth AJ, Carvajal M, Cooke DT, Schäffner AR, Steudle E,
Clarkson DT (1999) Diurnal variations of hydraulic conductivity and root pressure are
related to the expression of putative aquaporins in the roots of Lotus japonicus. Planta 210:
50-60.
(119) Henzler T, Steudle E (2000) Transport and metabolic degradation of hydrogen peroxide in
Chara corallina: model calculations and measurements with the pressure probe suggest
transport of H2O2 across water channels. J. Exp. Bot. 51: 2053-2066.
(120) Clarkson DT, Carvajal M, Henzler T, Waterhouse RN, Smyth A, Cooke DT, Steudle E
(2000) Root hydraulic conductance: diurnal aquaporin expression and the effects of
nutrient stress. J. Exp. Bot. 51: 61–70.
(121) Hose E, Steudle E, Hartung W (2000) Absisic acid and hydraulic conductivity of maize
roots: a study using cell-and root-pressure probes. Planta 211: 874-882.
(122) Freundl E, Steudle E, Hartung W (2000) Apolastic transport of abscisic acid through roots
of maize: effect of the exodermis. Planta 210: 222-231.
(123) Steudle E (2000) Water uptake by plant roots: an integration of views. Plant and Soil 226:
45-56.
(124) Steudle E (2000) Water uptake by roots: effects of water deficit. J. Exp. Bot. 51: 15311542.
(125) Barrowclough DE, Peterson CA, Steudle E (2000) Radial hydraulic conductivity along
developing onion roots. J. Exp. Bot. 51: 547-557.
(126) Zimmermann HM, Hartmann K, Schreiber L, Steudle E (2000) Chemical composition of
apoplastic transport barriers in relation to radial hydraulic conductivity of corn roots (Zea
mays L.). Planta 210: 302-311.
(127) Wei CF, Steudle E, Tyree MT (2000) Reply.... Water ascent in plants. Trends in Plant
Science 5: 146-147.
(128) Steudle E (2001) The cohesion-tension mechanism and the acquisition of water by plant
roots. Annu. Rev. Plant Physiol. & Plant Mol. Biol. 52: 847-875.
(129) Wei C, Steudle E, Tyree MT, Lintilhac PM (2001) The essentials of direct xylem pressure
measurement. Plant, Cell & Environment 24: 549-555.
(130) Hose E, Clarkson DT, Steudle E, Schreiber L, Hartung W (2001) The exodermis: a
variable apoplastic barrier. J. Exp. Bot. 52: 2245-2264.
(131) Miyamoto N, Steudle E, Hirasawa T, Lafitte R (2001) Hydraulic conductivity of rice roots. J.
Exp. Bot. 52: 1835-1846.
(132) Steudle E (2002) Aufnahme und Transport des Wassers in Pflanzen. Nova Acta
Leopoldina NF 85: 251-278.
(133) Steudle E (2002) Transport of water in plants. Environm. Contr. Biol. 40: 29-37.
(134) Ranathunge K, Steudle E, Lafitte R (2003) Control of water uptake by rice (Oryza sativa
L.): role of the outer part of the root. Planta 217: 193-205.
(135) Ranathunge K, Kotula L, Steudle E, Lafitte R (2004) Water permeability and reflection
coefficient of the outer part of young rice roots are differently affected by closure of water
channels (aquaporins) or blockage by apoplastic pores. J. Exp. Bot.
55: 433-447.
(136) Wan XC, Steudle E, Hartung W (2004) Gating of water channels (aquaporins) in cortical
cells of young corn roots by mechanical stimuli (pressure pulses): effects of ABA and
HgCl2. J. Exp. Bot. 55: 411-422.
(137) Ye Q, Wiera B, Steudle E (2004) A cohesion/tension mechanism explains the gating of
water channels (aquaporins) in Chara internodes by high concentration. J. Exp. Bot. 55:
449-461.
(138) Lee SH, Singh AP, Chung GC, Ahn SJ, Noh EK, Steudle E (2004) Exposure of roots of
cucumber (Cucumis sativus) to low temperature severely reduces root pressure, hydraulic
conductivity and active transport of nutrients. Physiol. Plant. 120: 413-420.
(139) Zhao CX, Deng XP, Zhang SQ, Shan L, Ye Q, Steudle E, Shan L (2004) Advances in the
studies on water uptake by plant roots. Acta Bot. Sin. 46: 505-514.
(140) Henzler T, Ye Q, Steudle E (2004) Oxidative gating of water channels (aquaporins) in
Chara by hydroxyl radicals. Plant Cell Environment 27: 1184-1195.
(141) Tyerman SD, Tilbrook J, Pardo C, Kotula L, Sullivan W, Steudle E (2004) Direct
measurement of hydraulic properties in developing berries of Vitis vinifera L. cv. Shiraz
and Chardonnay. Aust. J. Grape & Wine Res. 10: 170-181.
(142) Ranathunge K, Steudle E, Lafitte R (2005) Blockage of apoplastic bypass-flow of water in
rice roots by insoluble salt precipitates analogous to a Pfeffer cell. Plant Cell Environment
28: 121-133.
(143) Ye Q, Muhr J, Steudle E (2005) A cohesion/tension model for the gating of aquaporins
allows estimation of water channel pore volumes in Chara. Plant, Cell & Environment 28:
525-535.
(144) Lee SH, Chung GC, Steudle E (2005) Gating of aquaporins by low temperature in roots of
chilling-sensitive cucumber and chilling-tolerant figleaf gourd. J. Exp. Bot.
56: 985-995.
(145) Lee SH, Chung GC, Steudle E (2005) Low temperature and mechanical stresses
differently gate aquaporins of root cortical cells of chilling-sensitivce cucumber and resistant figleaf gourd. Plant, Cell & Environment 28: 1191-1202.
(146) Zhao CX, Deng XP, Shan L, Steudle E, Zhang SQ, Ye Q (2005) Changes in root hydraulic
conductivity during wheat evolution. J. Integr. Plant Biol. 47: 302-310.
(147) Schreiber L, Franke R, Hartmann KD, Ranathunge K, Steudle E (2005) The chemical
composition of suberin in apoplastic barriers affects radial hydraulic conductivity differently
in the roots of rice (Oryza sativa L. cv. IR64) and corn (Zea mays cv. Helix). J. Exp. Bot. 56:
1427-1436.
(148) Ranathunge K, Steudle E, Lafitte R (2005) A new precipitation technique provides
evidence for the permeability of Casparian bands to ions in young roots of corn (Zea mays
L.) and rice (Oryza sativa L.). Plant, Cell & Environment 28: 1450-1462.
(149) Ye Q, Kim YM, Steudle E (2006) A re-examination of the minor role of unstirred layers
during the measurement of transport coefficients of Chara corallina internodes with the cell
pressure probe. Plant, Cell & Environment 29: 964-980.
(150) Ye Q, Steudle E (2006) Oxidative gating of water channels (aquaporins) in corn roots.
Plant, Cell & Environment 29: 459-470.
(151) Garthwaite AL, Steudle E, Colmer TD (2006) Water uptake by roots of Hordeum marinum:
formation of a barrier to radial O2 loss does not affect root hydraulic conductivity. J. Exp.
Bot. 57: 655-664.
(152) Kim Y, Ye Q, Reinhardt H, Steudle E (2006) Further quantification of the role of internal
unstirred layers during the measurement of transport coefficients in giant internodes of
Chara by a stop-flow technique. J. Exp. Bot. 57: 4133 – 4144.
(153) Meyer CJ, Steudle E, Peterson CA (2007) Patterns and kineticsof water uptake by
soybean seeds. J. Exp. Bot. 57: 717 -732.
(154) Knipfer T, Das D, Steudle E (2007) During measurements of root hydraulics with pressure
probes, the contribution of unstirred layers is minimized in the pressure relaxation mode:
comparison with pressure clamp and high-pressure flowmeter. Plant Cell Environment 30:
845 – 860.
(155) Kim Y, Steudle E (2007) Light and turgor affect the water permeability of parenchyma cells
in the midrib of leaves of Zea mays. J. Exp. Bot. 57: in press.
Main invited lectures at meetings & seminar talks: 1998 – 2007
(as remembered)
Invited talk for the Gordon Research Conference on “Salt and Water
Stress in Plants” at Queen’s College, Oxford, UK, August 1998: “Stress effects
on cell and tissue water relations”.
International Rice Research Institute (IRRI), Los Banos, Philippines:
Invited lecture for the International Workshop on “Genetic Improvement of Rice for
Water-Limited Environments”, December 1998: “The physiological and anatomical
basis for an optimization of water uptake by roots of crop plants”.
Seminar talk: University of Western Ontario, London, Canada, October 12,
1999: “Water transport across roots: an integration of views“.
Invited talk: Department of Biology, University of Waterloo, Ontario, Canada,
October 1999:”Water transport across roots”.
Invited plenary lecture: VII Meeting of the Brasilian Society of Plant
Physiologists (CBFV), 18 to 22 July, 1999, in Brasilia: “Water absorption by roots“.
A two-days (20 & 21 of July) symposium was held on “Techniques for
measuring water transport across roots”.
Two seminar talks at the Charles University, Prague, Czech Republic,
October 2000: “Water transport across roots“ and “Is the cohesion/tension theory still
valid?“.
Seminar talk: Institut fuer Botanik II/III, Universitaet Freiburg, Germany,
January 10, 2000: “Ist die Kohaesionstheorie noch gueltig?“ (“Is the cohesion theory
still valid?”).
Seminar talk; Department of Renewable Ressources, University of Alberta,
Edmonton, Canada, July 27, 2000: ”Regulation of water uptake by plant roots”.
Seminar talk: Department of Botany, University of Hamburg, Germany,
January 2001: “Role of water channels (aquaporins) in plant water relations“.
Invited plenary lecture at the Main Meeting of the German Academy of
Natural Science (Deutsche Akademie der Naturforscher, Leopoldina), Halle,
Germany, April 2001. Title: “Aufnahme & Transport des Wassers in Pflanzen”
(“Uptake & Transport of Water in Plants”).
Seminar talk: School of Biology, University of Sussex, Brighton, UK, April
08, 2002: ”Water uptake by plants: regulation at the cell, root and whole plant level”.
Invited talk: International Symposium on Agricultural Plant Stress Response,
October 24 till 26, 2002, in Kwangju, South Korea. Title: “Water uptake by plant
roots: effects of water stress”.
Seminar talk: October 15, 2002, NMR Centre, Department of Molecular
Physics, Wageningen Agricultural University, The Netherlands:“The molecular
and biophysical basis of water transport: cell, tissue and root level”.
Seminar talk: University of Bonn, Germany, December 9, 2002: “Aufnahme
und Transport des Wassers in Pflanzen”.
Academic Dispute with Prof. Ulrich Zimmermann, Wuerzburg, in front of
the Physical-Medical Society at Wuerzburg (Physikalisch-Medizinische
Gesellschaft zu Wuerzburg), Germany, University of Wuerzburg: “Warum die
Baeume nicht in den Himmel wachsen” (“Why trees do not grow up to the heaven”).
Invitation by the Physikalisch-Medizinische Gesellschaft, Wuerzburg, June 26, 2003;
Three hours of dispute on the validity of the cohesion/tension mechanism of the
ascent of sap in plants.
Invited talk: Summer School of School of Biology, Faculty of Natural &
Agricultural Sciences of the University of Western Australia, Perth (Prof. Hans
Lambers), Rottnest Island, February 9 till 12, 2003: “Plant hydraulics: cell, organ,
and whole plant level”.
Invited talk: Sino-German Symposium of the German Science Foundation
(DFG) on “Extracellular Biopolymers of Plants and Their Function in Growth,
Development and Protection”, Chinese Academy of Sciences, Institute of Botany,
Beijing, September 18 till 22, 2004. Title: ”Role of apoplastic barriers during water
uptake by plant roots”.
• Three seminar talks in Beijing, China, in September 2004: (i) China
Agricultural University, State Key Lab of Plant Physiology & Biochemistry,
College of Biological Sciences, Sept.13: ”Water uptake by plant roots: an
integration of views”.
(ii) Chinese Academy of Agricultural Sciences, Sept. 16, 2004: “Water
transport in plants”; (iii) China Agricultural University, Dept. of Fruit Sciences,
College of Agriculture, Sept. 15:”The role of aquaporins in plant water relations”.
Invited talk: Workshop on “Water transport and aquaporins in grapevines” held
in Mallorca, Spain, Oct. 21 and 22, 2004, as part of the COST Action 858: “Water
uptake by plant roots: role of aquaporins”.
Technische Universitaet Bergakademie Freiberg: Einstein Year 2005:
“Einstein – Diffusion and Osmosis”; Freiberg, Saxony, Germany, June 08, 2005.
Seminar talk: Department Biochimie et Physiologie Moléculaire des Plantes
INRA/CNRS, Montpellier, France, December 01, 2005:” Gating of water channel
activity by plant cells“.
Invited talk at the Meeting of the Society of Experimental Biology at
Canterbury, UK, April 2006: “Gating of aquaporin activity of plant cells:
consequences at the cell, tissue and organ levels”.
Seminar talk: Department Organismic and Evolutionary Biology, Harvard
University, Mass., USA: “ Gating of aquaporin activity of plant cells” July 28, 2006.
Invited lecture: Gordon Research Conference on “Transport in Permeable
Media; 150 Years of Darcy’s Law” at Proctor Academy, Andover, New
Hampshire, USA, August 2006: ”Pore transport in plants: molecular, cell, organ &
whole plant levels”.
Seminar talk: Umweltforschungszentrum Leipzig/Halle, Germany:
“Porentransport des Wassers in Pflanzen: von der Ebene des Transportmoleküls zur
ganzen Pflanze”. (“Pore transport of water: from the level of individual transport
moelcules to the whole plant level”.). November 08, 2006.
Seminar talk: Department of Botany, Technical University of Munich in
Weihenstephan, Germany: “Die Regulation von Wasserfluessen in Pflanzen: vom
Aquaporin zur ganzen Pflanze.” (“The regulation of water flow in plants: from the
aquaporin to whole plant level.”). May 03, 2007.