0.4
Shikimate
Glycolysis
Cinnamate
1.2
8.0
Lactate
Submerge
1.0
Submerge
0.3
Control
Submerge
Submerge
4
DAS
5
6
7
0.0
1
2
3
4
DAS
5
6
0.0
1
7
3
Trp
6
7
0.0
1
2
3
4
5
DAS
0.4
G1P
GA3P
µ
mol/gDW
Submerge
1.0
µ
mol/gDW
4
µ
mol/gDW
µ
mol/gDW
20
2
0
4
5
DAS
6
7
2
3
Gly
Thr
GSH
4
5
DAS
6
7
2.0
3
4
5
DAS
6
7
1
2
3
6-PG
R5P
Submerge
0.3
0.2
0.1
0.1
0.0
2
3
4
5
DAS
6
7
R5P
0.3
Submerge
2OG
0.2
Pyruvate
0.1
0.1
0.0
1
2
3
4
5
DAS
6
7
PP pathway family
0.5
1
2
3
4
5
DAS
6
7
Submerge
6PG
GSSG
GABA
0.0
1
6
Control
Submerge
2.0
4
5
DAS
0.0
2
0.2
Control
1.5
3
3
G6P
8.0
0.0
1
Ala
2-OG
2.5
6
2
G6P
0.1
Gln
Glu(Gultamate) family
2-OG
Glu
30
1
2
0.2
µ
mol/gDW
Succinate
8
0
1
4.0
Glc
Succinate
Control
10
FBP
Isocitrate
SSA
Submerge
7
Control
Control
40
6
6.0
1
Pro
2OG
Fumarate
7
10
4
5
DAS
Submerge
0.3
Aconitate
TCA cycle
Nicotinate
Fumarate
3
Control
0
50
2
FBP
Cys
5
6
0.1
0.0
1
0.5
3PGA
Met
Citrate
15
4
5
DAS
7
0.3
Submerge
3
6
0.1
0.2
Control
2
4
5
DAS
Oxalo-Ac
Asn
Submerge
Control
Malate
10
3
µ
mol/gDW
Ornithine
20
2
µ
mol/gDW
Asparate family
Lys
Asp
0.2
0.0
1
His
Citrulline
25
7
Leu
Val
Citrate
6
Shikimate
PEP
Acetyl-CoA
Arg
0.1
Cinnamate
Tyr
PEP family
Pyruvate
Ala
4
5
DAS
Serine
Serfamily
1
0.2
µ
mol/gDW
Pyruvate family
2
Phe
Lactate
30
0.2
0.4
Coumarate
Shikimate pathway family
35
0.6
0.2
µ
mol/gDW
3
Control
0.3
µ
mol/gDW
2.0
µ
mol/gDW
0.2
1.0
µ
mol/gDW
µ
mol/gDW
µ
mol/gDW
µ
mol/gDW
2
0.8
0.3
0.3
2.0
0.0
1
0.4
GA3P
0.4
Control
Submerge
4.0
0.4
0.0
3PGA
1.0
Control
Submerge
6.0
0.6
0.1
PEP
0.5
Control
0.8
0.2
Pyruvate
3.0
Control
Control
7
TCA cycle
4
5
DAS
6
7
Phe
10
Trp
3.5
Control
Submerge
8
Tyr
5
PEP
0.5
Control
3.0
Submerge
Submerge
4
3PGA
1.0
Control
0.4
Control
Submerge
Submerge
0.8
Control
10
2.5
2.0
3
0.3
0.6
4
1.5
2
0.2
0.4
0.0
1
2
3
4
DAS
5
6
7
1
0.1
0
1
2
3
4
DAS
5
6
7
1
2
3
4
DAS
5
6
7
1
2
3
4
DAS
Phe
5
6
7
Shikimate
Pyruvate
PEP
3PGA
G1P
GA3P
Arg
FBP
Gly
Citrulline
5
6
7
His
3
4
DAS
5
6
7
Ser
20
18
16
14
12
10
8
6
4
2
0
Control
Submerge
2
3
4
DAS
5
6
7
6PG
R5P
R5P
0.3
Control
Citrate
Pro
Aconitate
GSH
Submerge
0.2
GSSG
0.2
0.1
0.1
0.0
Gln
Glu
2OG
Fumarate
µ
mol/gDW
Isocitrate
Malate
Asn
2
Cys
Oxalo-Ac
Asp
1
Glc
G6P
Thr
Leu
Val
Ornithine
Met
4
DAS
1
Ser
Acetyl-CoA
3
Serine
family
Cinnamate
Tyr
2
µ
mol/gDW
Ala
0
1
Trp
Lactate
2
0.0
Coumarate
PEP family
Lys
0.2
0.0
4
µ
mol/gDW
0.5
µ
mol/gDW
0
1.0
µ
mol/gDW
2
Submerge
8
6
µ
mol/gDW
µ
mol/gDW
µ
mol/gDW
6
Gly
12
Control
1
2
3
His
25
4
5
DAS
6
7
Control
Nicotinate
Succinate
Submerge
20
Glu
2OG
15
Ala
Pyruvate
µ
mol/gDW
GABA
SSA
10
5
0
1
2
3
4
DAS
5
6
7
PP pathway family
Pyruvate family
Ala
80
Pyruvate
3.0
Control
Submerge
60
Control
2.0
Submerge
Urea cycle family
40
0
Ornithine
Phe
Trp
3
0.0
1
2
3
Control
140
Coumarate
1.0
µ
mol/gDW
µ
mol/gDW
160
20
4
DAS
5
6
7
1
Lactate
Submerge
2
4
DAS
5
Cinnamate
6
Tyr
7
Shikimate
PEP
3PGA
G1P
Glc
120
100
Pyruvate
Ala
80
GA3P
FBP
G6P
µ
mol/gDW
60
40
Ser
20
Acetyl-CoA
0
1
0.7
2
3
4
DAS
5
6
Met
7
Arg
Citrulline
Gly
Thr
Leu
His
Control
0.6
Val
Submerge
0.5
Citrate
Citrulline
0.4
0.3
µ
mol/gDW
Lys
Asp
Ornithine
Pro
Aconitate
GSSG
GSH
Isocitrate
Malate
α-Ketogulutarate (2-OG) family
0.0
1
2
3
4
DAS
5
6
7
10
10
6
µ
mol/gDW
µ
mol/gDW
4
6
2
3
4
DAS
5
6
7
1
2
3
4
DAS
5
6
7
2
3
4
DAS
5
5
Control
6
6
7
Gln
Control
Submerge
Submerge
4
5
2
0
4
DAS
5
6
7
4
3
1.0
3
2
0.5
0.0
µ
mol/gDW
3
1.5
µ
mol/gDW
2
µ
mol/gDW
1
Asparate family
7
Submerge
2.0
10
1
Glu
Control
4
20
0
2-OG
2.5
2
0
1
Pyruvate
Ala
Submerge
8
µ
mol/gDW
0.0
40
GABA
SSA
Succinate
Control
0.5
Submerge
30
Submerge
8
1.5
1.0
2OG
Glu
Control
Submerge
2.0
Control
50
µ
mol/gDW
Control
GABA
60
Asp
12
70
Succinate
Nicotinate
Asn
Gln
Glu
2OG
Fumarate
Asn
2.5
R5P
Oxalo-Ac
0.2
0.1
6PG
Cys
2
1
0
1
2
3
4
DAS
5
6
7
1
0
1
2
3
4
DAS
5
6
7
1
2
3
4
DAS
5
6
7