Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
NpR4776g1
JSC1_RfpA
Anacy_2551g3
Anacy_4718g3
Cy7822_4053g2
N7104D_1016g3
L6406D_1154g2
c56D2_02270g2
c407D_01196g2
fdiDRAFT29700
WP009627289g3
Os7112_5903g3
C6303_3693g3
WP006632756g3
Cy7425_1390g3
WP017296986g2
WP_033374293
WP028089844g3
WP008316973g2
Ga0039499_10213
310F_3509
Sta7437_1656
WP_016871037
WP_016878855
WP_026722600
WP_017309337
WP016873240
Syn7502_01757
Nos7524_4790
Ch6605_4344g2
AM1_5997g1
JSC1_41260g2
IC523_04500g2
Ga0039500_1082
AIE75137
SyPlpA
MC7420_7957g2
WP_019501237
Chro_3029
WP_026793569
Gei7105_D5128
WP_033365958
M7113_3096g2
WP006632756g2
Anacy_2025
Os7112_5903g2
WP_036801164
WP_016952251
O6304_4080
O6304_4065g1
NpF3797
WP_017324438
WP_019500029
WP_009629217
RcaE
Mtestarum_GR
SyCcaS
JSC1_30280
Sta7437_2963
Cr9333_3812g2
Cr9333_1029
Cr9333_0815
Cy7425_0790
Mi7113_0260
Mi7113_1774g1
Aazo_0288
L8106_11447
S7335_5042g1
X7305_GR
Cri9333_4201
All1280g2
NpF6001
TePixJ
NpR6012g4
NpR1597g4
Oscil6304_4335
DRVMIYKFHADGNGQVIAESIYNNRLPSLLGLN-FPADDIPLSARELFLKLRVRSVVNVDTQEIGQIH…CHIEYLTAMGVKSSVVAPIL-LWGLLVSH
DRVKIYRFHGDGSGEVVAEAIRDQRLPSLLHHN-FPAGDIPREARELFLSVRQRSIVNVSTQQIGISP…CHVEYLTAMGVKSSLVVPIL-LWGLLVSH
ERVIIFRLFPNGRSQIVEEV-VSSEYAALKNYH-WEDEKWSQEILDCYWQGKPRIVPDVI--NDIWTS-CLVEYTTQGNIQSKIVAPIL…LWGVLVVH
DRVIVFRLFADGESQIVEEA-VSGELVSLKNRH-WDNEVWSQEILDYYWQGQPRIVTDVM--EDIWTD-CLVEYSIEGQVQSKIVAPIL…LWGVLVVH
DRVIIFRLYSDGGSRIIEES-VSTEFLPLKYCH-WDDETWSQDILNLYWQGQPRIVPDVM--NDIYTE-CLHEYSREGQIQSKIVAPIL…LWGILVVH
DRVIVFQVAQNGHSCILEEA-VAPDLPQLKAMQ-WDDETWSQDILEHYWQGQPRIVPDVM--EDHWTD-CLVEYSKAGQIQSKIVAPIL…LWGVLVVH
DRVIVFQVYHDGHSRIVEEA-VTPDLPSLKAMH-WEGETWPLDILEHYWQGQPRIVPDVM--DDIWTD-CLVDYAQAGQIQSKMVAPIL…LWGVLVVH
DRAIIFQLFDNGNSQIVEES-VHSNFLNLKALN-WDNEVWSQEILDCYWQGKPRIVPDVM--NDIWTE-CLVEYSLKGQIKSKIVAPIL…LWGVLVVY
DRAIIFQLFDNGNSQIVEES-VHSNFLNLKALN-WDNEVWSQEILDCYWQGKPRIVPDVM--NDIWTE-CLVEYSLKGQIKSKIVAPIL…LWGVLVVH
DRVIVFRLFADGRSKIAEEA-VSSEFVSLKNRH-WGNEIWSQEILDFYWQGKPRIVPDVM--NDLWTH-CLVEYSQEGQIQSKIVAPIL…LWGILVVH
DRVIVFRLFSYGDSQIVEEA-VSPEFTSLKSLH-WENELWSPAILDYYWQGKPRIVPDVM--VDVWTD-CLIPYSIEGQIKSKIVAPIL…LWGVLVVH
DRIVVFRLFGDGRSQIVEEA-VSSEFPALKDHH-WEDELWSPEILNRYWQGKPRIVPDVM--TDIWTD-CLVEYATVCQVQSKIVAPIL…LWGVLVVH
DRVIVFRLLGDGRSQIVQEA-VSNEFPVLKDRQ-WENEVWSQEILDGYWQGKPRIVPDVM--NDIWTE-CLVEYSREGKIQSKIVAPIL…LWGVLVVH
DRIIVFRLFGDGRSQIVEEA-VSSEFPALKDHH-WEDERWSQEILNRYWQGKPRIVPNVM--TDIWTD-CLVEYASVGQVQSKIVAPIL…LWGVLVVH
DRVIVFQLFPNGKSRIVEEA-VSSGLTVLKAGH-WEDEVWPQEILDYYWQGQPRIVADVM--DDRWTD-CLVGYSKQGEIVSKIVAPIL…LWGVLVIH
DRVIIFQVHSDGHSKIVEEA-VSESLPTLKGMR-WEDEVWSQDILDVYWRGQPRIVADVM--ADTWTD-CLVDYSQAGQIQSKIVAPIL…IWGVLVVH
DRVIVFRFCDLGRTCIFEEA-VAEDLPSLKYMN-WEDEQWSSEILQFYWQGQPRIVPDVM--NDPLTP-CLLDYSRQGQIQSKIVAPIL…LWGLLVIH
ERVIVFRLFPDGKSQIVEES-VANGYMTFKDSY-WEDEKWSQDILEYYWQGKPRIVLDVM--DDIWTD-CLKAYSRQGNIRSKIVAPIL…LWGVLVVH
DRVIVFQLFADGRSQIVEEE-VLGSLPALRTMH-WEDEVWSQDILALYWQGQPRIVPDVM--DDIWTD-CLVEYAQAGQIKSKIVAPIL…LWGVLVVH
DRAIIFQLFDNGNSQIVEES-VHSNFLNLKALN-WDNEVWSQEILDCYWQGKPRIVPDVM--NDIWTE-CLVEYSLKGQIKSKIVAPIL…LWGVLVVH
ERVIVFRLFPDGKSQIVEEA-VANGYMTFKDSY-WEDEKWSQDILEYYWQGKPRIVLDVM--DDIWTD-CLKAYSRQGNIRSKIVAPIL…LWGVLVVH
ERVLIFRMNPDGSGQVIEEA-VVPKYPVTDQMR-WEDEHFPEDCYEYYRQGIPRIVPDVA--TDEWAK-CLVEFMQEVGVKSKVVAPIV…VWGLLIVH
DRVLIFRLNQDGSGQIIEEA-VVPEYPMTYQMR-WVDECFPDDCYEYYRQGNPRILPDVA--KDEWGA-CLVEFMQQIGVKSKVVAPII…VWGLLIVH
DRALIFQLNQDGSGQIIQEA-VIPDYPVTNQMR-WLDECFPDECYEYYCQGNARIVPDVA--KDDWGA-CLVEFMQEVGVKSKVVAPIV…VWGLLIVH
DRALIFRLNEDGSGQVIKEA-VVPEYPVTEQMR-WRDEPLPDYCYDFYRQGNPRIVPNVA--IYDWAS-CLAEFLQQASVKSKIVAPIV…VWGLLIVH
DRALIFRLNQDGSGQVIKEA-VVPEYPMTSQMR-CTDECFPDDCYEYYRQGNARILPDVA--KDEWSD-CLVEFMQQIGVKSKVVAPII…VWGLLIVH
DRALIFRLHGNGTGQVIQEA-VRPEYPVTEQML-FPDECFPQECYEYYCQGQPRIVSDVF--AEDFSS-CLVEFMQKIGVKSKIVAPIV…VWGLLIVH
DRIIIFQLFPNGASQVVKEI-VKPPYPSIIGMN-WQDEHFSHEGFEYYINSNPRTVNDVF-EDNVFAD-CLKEFMSQAQIKSKIVAPII…LWGLLIVH
ERALIFRLMPNGSGVVLEES-VVPDYPSIKSRL-WQDECSPHELYQFYRDGNVRIVTDTQ--NDLWGE-CLAEFMAEAQVQSKIVAPIA…VWGLISIH
DRVTIFRVFPDRHIRVVEEV-VMPSYPSLLQRN-WEDECISQAEFDFYLHGHPHIVNDVL--QDAWSE-CLPEYVDTIGVKSKIVAPIL…LWGLLSIH
DRALLFRLMPGGGGIILEES-VLPNYPAMEELD-GEDQDFPPEYYEFYLHRQPRIVLDVD--QDDWSQ-CLKQFWLPAQVKSKIVAPIV…LWGLLVVH
DRALVFRLYANGTGCVIKEA-TLPQYPVTAEMR-FPEEQFPLECRTLYQRGQARAITNVD--NDILAD-CLVKFMHQIQVKSKLVVPIV…VWGLLILH
DRVLVYHILIDGTGKVIAEA-LTPGYESILEQS-FSVECFPTECYLRYAQGEIYALRDHE--ERQQPS-CLIQFMADMQIRAKLVIPIV…LWGLLIAH
DRVLIYRIFPNGSGKTITES-VIFPDLSILDQY-FTDETFSSECERFYEQRKVKAIDNIE--AAYPSD-CMRALMQQLQVKSKLIIPIF…LWGLMLAH
DRVLIYRIQENGLGTTVAES-VTGGHPSVMAMD-LDPESFPVECYQRYLNGYIYASTERL--PDCAAT-CATNCFTVAESKSRIVAPIV-LWGLLIVH
DRVLIHHIQEDGLGTTIAES-VVNGQPSVMQMD-LSPESFPPECYQRYLNGYIYASRDQL--PDCAIN-CAVQCFTVAESQSRIVAPIV-LWGLLIVH
DRVLVYQLFEDNRGRVIAEG-VASGFPQLLNSI-FPAEAFPPTCYQNYVQGKVYTLADRD--REDVLP-CMVDFMREYAIRAKLVVPIV-LWGLLIVH
DRVLVCKIARSGTGLVVAES-VSNPWNSLLNTY-FSAEAIPPECLQSYLEGHITYITDRK--SDPILP-CMSELMAKLQVEAILAVPII-LWGLLIAH
DRVVIYRLRSDGTGSAVAEA-VLPGWFSVLGQQ-FAAEVFPEDCHQLYCQGRIRGIADVE-REENIAP-CLVEFMRQFQVKAKLVVPIT-LWGLLIAH
DRVLVYRIYNDYTGCVISEA-VSPQWSSLLEKT-FSEEIFPSQCHSHYIQGKIASIANIN--KGNILP-CLVEFLTQFKVQAKLAVPII…LWGLLVVH
ERVLIFRLLENRLARVVSES-VAEGYPQSAGME-FPDEEFPRDCYDNYCRGLPRIVSDVE--TDRFAP-CLVDFMKQMQVRSKVVVPIL…LWGLLIAH
DRVVVYQVQLDGTGITVAEA-MMGEWTSILDQV-FPEETFPQQSHQQYLKGRIYTLTDRD--QGKIVP-CLAEFLAEIQVRAKLVVPII-LWGLLIVH
DRVLVYRVWSNGAGRVIAEA-VAPGCIKVLDIP-LPTETFPEEYRQRYAEGRIYTLTDLD--HEEVSP-CLANCLKQIQVRAKVVVPII-LWGLLIAH
DRTLVYRVFPEGTGAAIAES-VSPNRLKLLDIL-FPEEVFPEDTYERYIQGRVYALNDSEDENESIVP-CLVEFLADIEVRAKLVVPII…LWGLLIVH
DRVLIYRIWEDGTGCAITET-VSENYPCILGET-FPEEVFPREYHHAYTQGKTRAVTNID--QSDVES-CLADFVKQFGVKAKLVVPIL…LWGLLIAH
DRTLVYRVFPEGTGTAIAES-VSPNRLKLLDIL-FPEEVFPEENYERYIEGRVYALNDSEDANESIVP-CLVEFLADIQVRAKLVVPII…LWGLLIVH
DRVLIYRIREDGTGSTITET-VLSPYPAILGQT-FPEEVFPTEYHQAYIQGKSRTITNIE--QDDVEE-CLADFVKQFGVQAKLVVPII…LWGLLIAH
DRVLIYRLWEDGTGSAITET-VSPDYPCILGET-FPAEVFPKEYHQAYTQGKTRAITDIN--QSDVDS-CLAEFVKQFGVKAKLVVPIL…LWGLLIAH
DRVLVYRVWADGTGSAIAEA-VKPGWLKVLNRV-FPEEVFPPENYQRYIEGRICALVDRD--SGQTLP-CLVEFMKSIEVRAKLVVPIV-LWGLLIAH
DRLLIYRVDPDGTGIVVTHS-SDPLAPEINGNT-FPQETFSQEERDRYAQGEIVAIADTE--LVKHLP-CLAALIKTLKIKAKLVIPIL-LWGLLIAH
DRVLIYRLWEDGTGSAITET-VLPEYTKILGET-FPEEVFPREYHQAYSLGKTRAIANVE--QADVES-CLADFVKQFGVKAKLVVPIL…LWGLLIAH
DRVLIYQVFPNGTGRIISEA-VLPIYPETLGVD-FPEEVFPTDYQELYSQGRIQSIPNIHDPHLEIAE-CLIDFLEAWKVRAKLVVPIL…LWGLLIAH
DRVLIYRILSHGVGRTIAES-VLPEYPAILGMS-FPEEVLPLEYQELYRQGRMRAIEDVKAEDTGLTP-CLLEFVEQWCVRAKLTTPIL-LWGLLIAH
DRVLIYRLWSDGTGSAIHET-VLPPYPSILGQV-FPEEVFPAPYHRAYSLGKILTIANVE--QCEMEV-CLRDFVKQFGVRAKLVVPII…LWGILIAH
DRVLIYHVLPDGTGKTISES-VLPDYPTLMDLE-FPQEVFPQEYQQLYAQGRVRAIADVHDPTAGLAE-CLVEFVDQFHIKAKLIVPIV…LWGLLIAH
DRVLIYQVFANGTGKSISEA-VLPEYPAVLGVV-FPEEVFPQEYRELYGGGRVKAIADIHSPDAGLAE-CLVEFMDEWTVKAKLVVPIL…LWGLLIAH
DRVLIYRIWQDGTGSAITES-VNANYPSILGRT-FSDEVFPVEYHQAYTKGKVRAINDID--QDDIEI-CLADFVKQFGVKSKLVVPIL…LWGLLITH
DRVLIYQVLPDGTGKPISEA-VLPAYPEILGME-FPEEVFPKEYQQLYAQGRVRAIADVRDPAAGLAE-CLVEFIEQFDIRAKLIVPIL…LWGLLIAH
DRVLIYQVLANGTGKPISEA-VLPEYAPILGIE-FPEEVFPKDYQELYAAGRVQAIANVHDPQMGLAE-CLVDFMEEWKVQAKLVVPIL…LWGLLIAH
DRVLIYRVLSDGSGCTITEA-VLPGLPVLVGIP-FPEEVFPVEYQELYKLGKVQSIENVEQAYSEETP-CLVEFLRQFAVKAKLIVPIV-LWGLLIAH
DRVLVYRILADGTGSAIAES-VNPQWSSILEKT-FPSEVFPEEYRELYSKGRIRAISDVT--NGELAP-CLTDFVQNFNVKAKLVVPII-LWGLLIAH
DRVLLYQILPNGTGMVVTEA-VNSGWKPILGQV-FSPEVFPEEYHQQYVQGRISATADVE--NDEVLP-CLIEFLQNLQVKAKLVVPII-LWGLLIAH
DRVLIYRLWPNGTGSGVAEA-ALPGLPQIMGYT-FPEEVFPEAARQLYCRGQIRELVDVE-QDQQISA-CMVEFLQQFWVKAKLVVPIL-LWGLLIVH
DRVLIYRVWPDGKGCTVTEA-VQPGWPAILGMS-FAEEVFPLKYQELYRQGQVHAIADVEQAYAEMTP-CLLEFLHPWGVKAKLVVPIL-LWGLLIAH
DRVLIYRLWPDGTGSGVTEA-IVPGWSTVLGQV-FEAEVFPQEYHQLYASGRILAIKNVE--DAEISP-CMVEFLQQFEVKSKLVVPIL-LWGLLVAH
DRVLIYRLWEDGTGSAITET-VSPDYPCILGRI-FPAEVFPREYHHAYTQGRTTAITNVE---SDVNS-CLAEFLNQFGVKAKLVVPIL…LWGLLIAH
DRALIYQLYADGTGKVIAEA-VNSDWTAILDQT-FPTETFPAEIYEDYLQGKVGIISNVD--TENILP-CLVEFVQQFQVKSKVTLGII…LWGLLIIH
DRVLIYQVLPDGTGKAVSEA-VQAPYPSIIEIP-FPEEVFPEDYQALYAEGRIRAVTDVRSPKANIAE-CLVEFLAQWDVRSKLIVPIV…LWGLLIAH
DRVLIYQVFTNGTGMPIREA-VLPEFKPILGVK-FPEEVFPEDYRQLYSQGRVIAIADVRSTDANLAE-CLIEFMDEWQIKSKLVVPIL…LWGLLIAH
ERALIYRFGWNRCGTVVQEA-VIGDYPSLLGRS-FSAEVLPKEYHQMYSQGRVGIIHNLD--QDQLSP-CMVEFMQEFEAKAKLVVPIR-LWGLLVVH
DRVVVYQFAPDMSGKIVAES-VKPGWKIALGAD-IQDNCFQSGAGADYRQGHKRAIANIY--TAELTD-CHLRLLEQFQVKANLVVPIL…LWGLLIAH
DRVVVYQFDSEMIGTIMAES-VEPGWRVSLGVE-IYDTCFQTGKGAEYYQVNKRAIANIY--EAGLTD-CHIRLLEQFEVKANLVVPIL…LWGLLIAH
DRVIVYAFDDNYVGTVVAES-VAEGWPQARDQV-IEDPCFREHWVEAYRQGRIQATTDIF--KAGLTE-CHLNQLRPLKVRANLVVPMV-LFGLLIAH
DRVAVYRFNPNWTGEFVAES-VAHTWVKLVGPD…WEDTHLQETQGGRYAQGENFVVNDIY--QVGHSP-CHIEILEQFEVKAYVIVPVF-LWGLLAAY
DRVTIYRFRADWSGEFVAES-LAQGWTPVREIV…VADDYLQETQGRNFANGKSIVIKDIY--SANYSI-CHIALLELMQARAYMIVPIF-LWGLLAAY
DRVGVYRFKPDWSGNFVGES-VSPGWNPLMGNL…IADTHLQETQGGRYRENQALAVNDIY--NVGHAQ-CHIELLETFQAKAYIIVPIL-LWGLLAAY
Figure S1. Sequence alignment of CBCR domains. The Asp-motif lies between the conserved
positions in orange. Key acidic residues of the Asp-motif are in blue, as is the Cys residue
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
required for covalent attachment to chromophore. Trp residues conserved in far-red CBCRs are
in bold. Far-red/orange sequence names are in bold, and far-red/red ones are underlined.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
JSC1_41260g2
Ga0039500_10822
O6304_4080
All1280g2
NpF6001
L8106_11447
Cr9333_3812g2
WP_026793569
NpR1597g4
Mi7113_1774g1
NpR4776g1
Oscil6304_4335
WP_019500029
Cy7425_0790
Chro_3029
Cr9333_1029
Mi7113_0260
SyCcaS
NpF3797
WP_009629217
WP_036801164
Aazo_0288
Anacy_2025
WP_016952251
AIE75137
SyPlpA
IC523_04500g2
Anacy_2551g3
WP028089844g3
310F_3509
Cri9333_4201
Cr9333_0815
Gei7105_D5128
WP_017324438
S7335_5042g1
RcaE
JSC1_30280
X7305_GR
Mtestarum_GR
Sta7437_2963
JSC1_RfpA
WP_033365958
M7113_3096g2
Sta7437_1656
WP_026722600
WP_017309337
WP_016871037
Syn7502_01757
WP016873240
WP_016878855
Ch6605_4344g2
Nos7524_4790
WP009627289g3
Cy7425_1390g3
Cy7822_4053g2
c56D2_02270g2
Ga0039499_10213
c407D_01196g2
WP_033374293
N7104D_1016g3
L6406D_1154g2
Os7112_5903g3
WP006632756g3
Anacy_4718g3
C6303_3693g3
fdiDRAFT29700
MC7420_7957g2
WP_019501237
WP017296986g2
WP008316973g2
O6304_4065g1
AM1_5997g1
ILIKEIHHRVKNNLQVIS…CLNLDTAIPCGLLLNELITNAI…LSVHDNGICMSAD…---LGLKIAYDL-ALQLQGTLTLERT--QGTRFQL-IF
VLLREIHHRVKNNLYVIS…TLSLESAIPCGLLINELVTNSF…LKISDNGIGIPND…---LGLRLVSIL-ADQLEASLEVDCS--NGTSFTL-IF
LLLKEVHHRVKNNLQVIS…SLNLDTAIPCGLLLNELVSNSL…LIVRDTGRGLPEG…---LGLRLVRAL-TRQLRGTLDIENS--HGACFQI-TF
VLLKEVHHRVKNNLQIVS…ELNLDQAIACGLIINELISNSL…LTIKDNGIGLPDN…---LGLSLVHDLVTEQLEGTVSIERQP--GTTFKI-QF
VLLKEVHHRVKNNLQIVS…NLNVDQAIACGLVINELVSNAL…MTIQDNGIGLPAN…---LGLSLVYDLVTEQLEGNITLERNHGTGFKIKFKQL
VLLKEIHHRVKNNLLVVA…FLNIETAHPCGLIVNELVSNVF…LTVQDNGIGFSDN…---LGMELICTL-TTQLEGHIELIR--GEGTTFNV-TF
ILLKEIHHRVKNNLLVVS…ELNIETANPGGLIVNELVSNAF…LIIKDNGIGFPPN…---LGLQLVCTL-TEQLEGEINLTKE--DGTAFNL-TF
VLLKEIHHRVKNNLLVVS…GLNIETAQPCGLIVNELVSNTL…LTVRDNGIGFPAG…---LGMELVCTL-TEQIEGTITLNQE--NGTLFTL-SF
QLVAGIAHEINNPISFIY…PLVCYGAQMNQVFMN-ILNNAI…IWIADNGCGIPEI…GTGLGLSISYQIIVEKHGGNIKCVSEPGKGCEFWI-EI
QLVAGIAHEINNPVNFIY…KVQCYAGQLNQVFMN-ILANAI…ILISDNGPGMTQE…GTGLGLSISYQIVVEKHGGRLECISAPGQGTVFLI-SL
QLVAGVAHEINNPVNFIY…LVECYAGPLNQVFMN-VLSNAI…IRIADNGSGIPEA…GTGLGLSISYQIVVDKHGGVFKCDSQPGLGTEFWI-EI
QLVAGVAHEINNPVNFIY…LVDCYAAQMNQVFMN-ILSNAI…IRICDNGPGIPDE…GTGLGLSISYQIVVEKHGGSLTCLSQPGQGTEFRI-EL
TLASGIAHDLNNVLAPIL…DVYGDATQLHQVLIN-LVVNAR…IAIADTGVGIPPD…GTGLGLSTVLGI-VKSHGGFINVLSQVGQGTQFKL-FL
QFAYIASHDLQEPLRMVT…TIHADPTQLTQLFQN-LISNAI…FSIEDNGIGIDPQ…GTGIGLAVCKKI-IERHRGRIWVQSELGQGATFYF-TL
QFASIASHDLQEPLRKIQ…SIHADPLQMRQLLQN-LIGNAL…ITVEDNGIGFDQK…GTGIGLAICRKI-VERHGGSIAAESKPSQGAKFIV-TL
QFAYIASHDLQEPLRKIQ…TIDADPLQMRQLIQN-LISNAL…IVVEDNGIGFDEK…GTGMGLAICRKI-VEQHNGSITAKSQLGHGAKFIV-TL
EFAYVASHDLQEPLRKIQ…ILEADPIQMRQLLQN-LIGNAL…IFIEDNGIGFDEK…GTGMGLAICRKI-VERHDGTITAQSSPNLGATFII-TL
RFFSMASHEFRTPLSTAL…KALVDERLVRSILSN-LLSNAI…FEVTDQGIGISPE…GTGLGLMVAKKC-VDLHSGSILLKSAVDQGTTVTI-CL
RFFSMASHEFRTPLSTAL…PASVDERLLRSILSN-LLLNAI…LQVKDRGIGIPLA…GTGLGLVVVKKC-VDLHQGHINITSEIGIGTTCAI-AL
RFFSMASHEFRTPLSTAL…NVYLDEKLWRSLLAN-LLSNAI…LTVADRGIGIPLE…GTGLGLVVVKKC-VDLHQGTIEIKSEVGLGTICRI-AI
RFFSMASHEFRTPLSTVL…RICLDEKLMRSIMAN-LLSNAI…IQITDNGIGIAPD…GTGLGLVVVKKC-VDLHDGQIDLFSQLNLGTTVKV-TL
RFFSMASHEFRTPLSTAL…QAYLDEKLLRSILGN-LLSNAI…IQISDHGIGISTT…GTGLGLVVVKKC-VDLHGGHLNIVSELGHGTTVTV-TL
RFFSMASHEFRTPLSVAL…KAYLDEKLLRSILGN-LLSNAI…IKICDQGIGIPTN…GTGLGLMVVKKC-IDLHQGSLSIISASGHGTTVTV-IL
RFFSMASHEFRTPLSVTL…KAYLDEKLLRSILGN-LLSNAI…IKISDQGIGIPVT…GTGLGLVVVQKC-VDLHGGSLKIVSDSGNGTTVTV-IL
RFISTTSHEFRTPLAIIS…IVAFDPKLLRRILTN-LLSNAI…FSVRDYGIGIDAE…GTGLGLSIVKKS-VELHGGAIAITSELGQGSCFEV-DL
RFISTTSHEFRTPLAIIS…IVAFDPKLLRQILTN-LLGNAI…FSVQDHGIGIGPE…GTGLGLPIVKKC-AELHGGMITVTSQLGQGSRFEV-EL
RFIAMTSHEFRTPLAVIA…TAHLDKKLLRQMLSN-LLSNAV…FLITDQGIGISKQ…GTGLGLTIVKKC-VELQGGKISVASEIEAGTTFTV-EL
RFITMASHEFRTPLAIIA…IVQLDKKILQQILAN-ILTNAI…FKIKDSGIGIPEE…GTGLGLSIVKKC-VDLHKGEISFDSKLGQGTTFTI-II
RFVSMVSHEFRTPLAIIS…FAEFDPKIIRQVLTN-LLTNAI…FIVQDYGIGISET…GTGLGLAIVKKC-VDQHQGKITLESKLNQGTIFKV-TI
RFVSMVSHEFRTPLAIIS…FAEFDPKIIRQVLTN-LLTNAI…FIVQDYGIGISET…GTGLGLAIVKKC-VDQHQGKITLESKLNQGTIFKV-TI
RFVSTVSHEFRTPLSTIL…FVEFDNQLMGQIINN-LLTNAV…LSICDQGIGIPHS…GNGLGLSIVKKA-IELHGGNIIVESEVGVGTTFTV-CI
RFVTIASHEFRTPLAIIL…NVEIDEKLLRQMLTN-LLSNAV…FQIQDQGIGIPVT…GTGLGNTIIKNA-VEAHGGMITIESEVDVGTTFTV-SL
EFVSIVSHELRTPLTSMR…KLWVDGDRTIQVLTN-LISNAI…VQVCDRGRGIPSD…GTGLGLAICRRL-VLEHGGRIWAENRPGGGTRMSV-VL
EFITIVSHELRTPLTSIH…EISVDPDFMIQAFTN-LLSNAI…FEVRDEGQGIPSD…GTGLGLAICRKI-IEQHEGQIWVESSIGKGSSFYF-IL
EFIAVVSHELRTPLTSIH…SIWADPDYIVRALTN-LLGNAI…FCVQDNGQGIPHN…GTGLGLTICRKI-VEQHGGKIWAESQLGKGSRFSF-TV
EFISIISHELRTPLTSLH…SVWADADYILQALTN-LLSNAI…FRVRDEGQGIPAD…GTGLGLTICRKI-IEQHNGRIWAESTPGCGSTFAF-TL
EFISVVSHELRTPLTSLH…LIWADSDYIVQALTN-LLSNAI…FQVRDQGQGIPAD…GTGLGLTICRKI-IEQHDGRIWAESKLGEGSTFFF-TV
EFISVVSHELRTPLTSIH…IVWADRDYIVQALTN-LLSNAI…FAVQDQGQGIPED…GTGLGLAICRQI-VEGHGGQIWVESCLDKGSTFYF-TL
EFISVVSHELRTPLTSIH…VVWADRDYIVQTLTN-LLSNAI…FEVKDRGQGIPTS…GTGLGLAICRQI-IEGHGGKIWAESCLEEGSTFYF-TL
EFISVVSHELRTPLTSIH…VVWADRDYIVQTLTN-LLSNAI…FEVKDQGQGIPAN…GTGLGLAICRHI-IEEHGGKIWAESSLGGGSTFYF-TL
EFVSIVSHELRTPLTSIR…TLWADPDRIIQTFTN-LLNNAI…FRIKDQGVGIPAD…GTGLGLTICRGI-VQQHQGQIWVESKLGEGSTFCF-SL
EFISVVSHELRTPLAAIH…QVWADPDRIIQTLVN-LVSNAI…FKVQDQGRGIPYD…GTGLGLPICRSI-VEQHGGKIWAESVLGKGSVFYF-TL
EFISIVSHELRTPLSSIR…QVWADRDRILQTLVN-LVSNAI…FQVKDQGRGIPAD…GTGLGLAICKSI-VQQHGGKIWVESVVGEGSNFYF-TL
EFISVVSHELRTPLTSIR…TLWAAPDAIIQTLTN-LLSNAI…FTVKDRGRGIPED…GTGLGLSICKRI-VQQHGGRIWVESSLGEGSTFYF-TL
EFISIVSHELRTPLTAIR…QISAEADSIIRALTN-LLSNAI…FKIRDQGRGIPPD…GTGLGLAICKNI-VQQHGGHIWVESVLGEGSTFYF-TL
EFISIVSHELRTPLTAIR…QISAAADSIIQTLIN-LLSNAI…FKVRDQGRGIPPE…GTGLGLAICKNI-VQQHGGCIWVESVLGEGSTFYF-TL
EFISIVSHELRTPLTAIR…QIWAAPDAIVQTLIN-LLSNAI…FAVKDQGRGIPPE…GTGLGLSICKSI-VDQHGGRIWVESLLGEGSTFYF-IL
EFISIVSHELRTPLTAIR…QIWAAPDAIVQTLIN-LLSNAI…FAVKDQGRGIPPE…GTGLGLSICKSI-VDQHGGRIWVESLLGEGSTFYF-IL
EFVSIVSHELRTPLTAIR…QIWVAPDAIVQTLIN-LLSNAI…FTVRDQGRGIPSD…GTGLGLAICKSI-VKQHGGKIWVESRVGEGSTFYF-TL
EFISIVSHELRTPLTAIR…QIWAAPDAITQTLIN-LLGNAI…FFVRDNGRGIPSD…GTGLGLAICKTI-IRQHGGKIWVESVLGEGSTFYF-TL
EFLASMSHELRTPLNAIL…NIRVDDLRIRQVLIN-LLSNAV…LSIVDTGIGIAPE…GTGLGLALVKRI-VELHGGTVTVESQVDRGSCFRV-CL
EFLASMSHELRTPLNAIL…DIAVDERRMRQVLIN-LLNNAV…FAVIDTGIGIAPE…GTGLGLALVRQI-AELHGGNVTVSSEVGKGSCFTV-RL
EFLANMSHELRTPLNAIL…DLKIDERRIRQVLIN-LLNNAV…FAVIDTGIGITPH…GTGLGLALVKRI-VEMHGGQVKATSDFGVGSCFTI-EL
EFLANMSHELRTPLNAIL…DVWVDERRIRQVLIN-LLNNAV…IAVTDTGIGIPSH…GTGLGLALVKRI-VELHGGEVGVTSTEGAGSCFTI-DL
EFLANMSHELRTPLNAIL…MLTLDERRIRQVIIN-LLNNAV…FAVKDTGIGIAAE…GTGLGLALVKRL-VDLHGGEVSVTSELGVGSCFSV-DL
EFLANMSHELRTPLNSIL…KIMLDERRIRQVLIN-LLNNAV…IAVIDTGIGISAE…GTGLGLALVKRL-VEIHGGTVELTSELGVGSCFAI-NL
EFLANMSHELRTPLNSIL…KIMLDERRIRQVLIN-LLNNAV…IAVIDTGIGISAE…GTGLGLALVKRL-VEIHGGTVELTSELGVGSCFAI-NL
EFLANMSHELRTPLNSIL…EIMLDERRIRQVLIN-LLNNAV…IAVIDTGIGISAE…GTGLGLALVKRL-VEIHGGTVELTSELGVGSCFAI-NL
EFLANMSHELRTPLNAIL…DIVLDERRIRQVLIN-LLNNAV…FSVIDTGIGITPE…GTGLGLAVTKRI-VELHGGQVGVSSEEGKGSCFMI-DL
EFLANMSHELRTPLNAIL…EITLDERRIRQVLIN-LLSNAV…FAVTDTGIGITPE…GTGLGLALVKRI-VELHRGQVGLTSDVGVGSCFTV-EL
EFLANMSHELRTPLNTIL…EITLDERRIRQVLIN-LLNNAV…FAVTDTGIGITPE…GTGLGLALVKRI-VELHGGQVGLTSAVGVGSCFTF-DL
EFLANMSHELRTPLNAIL…DLLIDERRMRQVLIN-LLNNAV…ISVIDTGIGIAPE…GTGLGLALVKRI-VELHGGQVLLTSTVGVGSCFTI-DL
EFLANMSHELRTPLNAIL…DLLIDERRMRQVLIN-LLNNAV…IAVIDTGIGIAPE…GTGLGLALVKRI-VELHGGQVGLTSTVGVGSCFTI-DL
EFLANMSHELRTPLNAIL…DLFVDERRIRQVLIN-LLNNGV…ITVIDTGIGISPE…GTGLGLALVKQI-VELHGGQVGLTSELGVGSCFMI-DL
EFLANMSHELRTPLNAIL…DLFVDERRIRQVLIN-LLNNAV…FAVRDTGIGISPE…GTGLGLALVKRI-IELHGGLVGLTSELGVGSCFTI-DL
EFLANMSHELRTPLNAIL…HLLVDERRIRQVLIN-LLNNAV…IAVKDTGIGIAPE…GTGLGLALVKRI-VELHGGRVGLSSELGVGSCFTI-EL
EFLANMSHELRTPLNAIL…TILGDERRLQQVLIN-LLSNAV…ISVSDTGIGIAKE…GTGLGLALVRRI-VELHGGWVTVESEIGKGSCFTV-RL
EFLANMSHELRTPLNAIL…PVFMDERRMRQVLIN-LLSNAV…FNVIDTGIGIASK…GTGLGLALVRRI-VEMHGGKVSVESEVGQGSKFQV-NI
EFLANMSHELRTPLNVIL…YIVADPNKLRQVLIN-LLGNAI…FAVEDTGTGIAPA…GTGLGLAISRSL-VQLMGGSLTVSSRLGQGSTFCF-SL
EFLANMSHELRTPLNVIL…YIVADPNKLRQVLIN-LLNNAI…FQVIDTGVGIPSE…STGLGLAISRSL-VKLMGGELTVNSAPDQGSTFQF-AI
EFLANMSHELRTPLNAIL…MIMSDESKLRQVLIN-ILGNAI…IEIADTGSGIAPE…GTGLGLPISRKF-IQLMGGDIHVSSTVGVGTVFSF-EI
IFLANMSHELRTPLNAIL…YIRTDPGKLRQVLMN-LLSNAI…FTVSDTGSGIAPE…GTGLGLAISRQF-VQIMGGNLTAHSIHNQGTTLTF-NI
Figure S2. Sequence alignment of His kinase bidomains. Conserved positions are in orange.
The presumptive phosphoacceptor His residue is in bold. Far-red/orange sequence names are in
bold, and far-red/red ones are underlined.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S3. Characterization of purified proteins. The indicated proteins were subjected to
SDS-PAGE followed by semi-dry transfer to PVDF. Transferred proteins were visualized by
Amido Black (top) and by zinc blotting (bottom; PEB is not detected).
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S4. Additional characterization of far-red/orange CBCRs. (A) Absorption spectra are
shown for His-tagged Anacy_4718g3 using the color scheme of Fig. 3A. (B) Normalized
photochemical difference spectra are shown for intein-CBD (brick red) and His-tagged (teal)
constructs of Anacy_4718g3. (C) Absorption spectra are shown for Anacy_4718g3 during
incubation in darkness after far-red illumination. (D) Normalized photochemical difference
spectra are shown for Anacy4718g3 (brick red), Anacy2551g3 (dark blue), and
Cyan7822_4053g2 (desert rose). (E) Normalized absorption spectra are shown for
Anacy_2551g3 (dark blue) and Sta7437_1656 (teal circles) in the far-red-absorbing state. (F)
Normalized absorption spectra are shown for Sta7437_1656 (teal) and RcaE (dark green) in the
red-absorbing 15E state. (G) Forward (blue) and reverse (orange) photochemical difference
spectra are shown for WP_016871037. The reverse difference spectrum was acquired after
extensive illumination, whereas the forward reaction readily proceeded to completion. (H)
Normalized photochemical difference spectra are shown for Sta7437_1656 (teal circles) and
WP_016871037 (violet squares, forward reaction).
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S5. Characterization of additional CBCRs. (A) Absorption spectra are shown for
Oscil6304_4080 before (blue trace) and after (orange) illumination with green light (550±35
nm). (B) Normalized absorption spectra are shown for the green-absorbing 15Z states of
Oscil6304_4080 (blue), green/red CBCR RcaE (dark green), and green/blue CBCR
Oscil6304_4336g2 (desert rose). (C) Normalized photochemical difference spectra are shown for
Oscil6304_4080 (blue) and RcaE (dark green). (D) Absorption spectra are shown for
Nos7524_4790 in the 15Z (blue) and 15E (orange) configurations. Photoconversion was
triggered using violet and red light (400±35 nm and 650±20 nm, respectively). (E) Normalized
native difference spectra are shown for Nos7524_4790 (blue) and RcaE (dark green). (F)
Absorption spectra are shown for Nos7524_4790 in the red-absorbing 15E state after dilution
into acidic guanidinium chloride in darkness. Spectra are shown before (orange) and after (blue)
illumination with white light.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S6. Assignment of biliprotein chromophore structure using acidic denaturation. (A)
A cartoon schematic shows effects of denaturation on protein structure and reversibility of
photoconversion. Denatured 15E bilin can be photoconverted to the 15Z configuration by white
light in the absence of protein structure. (B) Examples of denatured spectra are shown for the
indicated bilins under denaturing conditions (corresponding to the lower left-hand corner in
panel A). Peak wavelengths for the two chromophore bands are shown for each, along with the
ratio of intensities for the two bands. Where the 15,16-double bond is present, bilin spectra are
for the 15Z configuration. Covalent adducts are in blue, and noncovalent species are in red.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S7. Characterization of far-red CBCRs under denaturing conditions. (A) Absorption
spectra are shown for Anacy_2551g3 in the far-red-absorbing photostate after acid denaturation
before (orange) and after (blue) 1 min illumination with white light. (B) Absorption spectra are
shown for Cyan7822_4053g2 in the far-red-absorbing photostate after acid denaturation under
the conditions of panel A. (C) A detail view is shown for WP_016871037 as purified after acid
denaturation under the conditions of panel A. (D) A detail view is shown for WP_016871037
after far-red illumination and subsequent acid denaturation under the conditions of panel A.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Figure S8. Domain architectures of cyanobacterial far-red-responsive histidine kinases.
Jellybean domain-architecture cartoons are shown for the three known classes of cyanobacterial
far-red histidine kinases: knotted phytochromes, knotless phytochromes, and far-red CBCRs. For
variable or repeated domains in far-red CBCRs, the range present for a given type of architecture
is indicated by subscript.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Table S1. Accession information for far-red/orange CBCRs1
Sequence
Domain architecture
DOE-IMG accession
Organism
WP008316973g2
LEP6406DRAFT_2712
Leptolyngbya PCC 6406
C56DFT2_02270g2
HAMP-PAS10-CBCR-CBCRHK-REC
PAS2-CBCR-CBCR-HK-REC
cya56DRAFT2_02270
c407DFT_01196g2
PAS4-CBCR-CBCR-HK-REC
Ga0039498_104087
Ga0039499_10213
PAS4-CBCR-CBCR-HK-REC
Ga0039499_10213
WP017296986g2
PAS7-CBCR-CBCR-HK-REC
Lepto7104DRAFT_1307
Cy7822_4053g2
CBS2-PAS3-CBCR-CBCR-HKREC
CBS4-GAF-CBCR-HK-REC
Cyan7822_4053
Planktothrix agardhii
NIVA-CYA 56/3
Planktothrix rubescens
NIVA-CYA 407
Planktothrix prolifica
NIVA-CYA 406
Leptolyngbya PCC 7104
(Nodosilinea nodulosa)
Cyanothece sp. PCC 7822
CBS4-GAF-PAS10-CBCRCBCR-HK-REC
CBS4-CBCR-PAS-CBCRCBCR-HK
CBS3-CBCR-PAS3-CBCRCBCR-HK
CBS3-CBCR-PAS3-CBCRCBCR-HK
CBS4-CBCR-PAS11-CBCRCBCR-HK
CBS2-CBCR-PAS7-CBCRCBCR-HK-REC
CBS3-CBCR-PAS2-CBCRCBCR-HK-REC
CBS4-CBCR-PAS3-CBCRCBCR-HK-REC
CBS2-CBCR-PAS3-CBCRCBCR-HK-REC
CBS4-CBCR-PAS11-CBCRCBCR-HK-REC
CBS4-CBCR-PAS5-GAFCBCR-HK-REC
CBS-CBCR-PAS-GAF-PAS4CBCR-CBCR-HK-REC
LEP6406DRAFT_1154
C6303_3693g3
L6406D_1154g2
Anacy_2551g3
WP028089844g3
310F_3509
WP006632756g3
N7104D_1016g3
fdiDRAFT29700
Anacy_4718g3
WP009627289g3
Os7112_5903g3
WP_033374293
Cy7425_1390g3
Cal6303_3693
Anacy_2551
131C_1565
310F_3509
MicvaDRAFT_3059
Lepto7104DRAFT_1016
fdiDRAFT29700
Anacy_4718
Pse7429DRAFT_2072
Osc7112_5903
Spi9445_1327
Cyan7425_1390
Calothrix parietina PCC
6303
Leptolyngbya PCC 6406
Anabaena cylindrica PCC
7122, PCC 7938
Anabaena circinalis
AWQC131C
Anabaena circinalis
AWQC310F
Microcoleus vaginatus
FGP-2
Leptolyngbya PCC 7104
(Nodosilinea nodulosa)
Tolypothrix sp. PCC 7601
Anabaena cylindrica PCC
7122, PCC 7938
Pseudanabaena sp. PCC
7429
Oscillatoria nigro-viridis
PCC 7112
Spirulina subsalsa PCC
9445
Cyanothece sp. PCC 7425
1. Abbreviations for domains are as follows. CBS: cystathionine--synthase; CBCR:
cyanobacteriochrome domain; PAS: Per-ARNT-Sim; GAF: cGMP-specific phosphodiesterases,
cyanobacterial adenylate cyclases, and formate hydrogen lyase transcription activator FhlA;
HAMP: Histidine kinases, Adenylate cyclases, Methyl-accepting proteins and Phosphatases; HK,
histidine kinase bidomain; REC, response regulator receiver domain. Numbers indicate multiple
domains of a given type in tandem, and CBCRs belonging to the far-red cluster are indicated in
bold.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Table S2. Accession information for far-red/red CBCRs1
Sequence
Domain architecture
DOE-IMG accession
Organism
Sta7437_1656
Sta7437_1656
WP_016871037
CACHE-HAMP-PAS2-CBCR-PASHK
TPR-GAF-PAS7-CBCR-PAS3-HK
WP_026722600
WP_017309337
WP_016878855
GAF-PAS6-CBCR-PAS2-HK
GAF-PAS4-CBCR-PAS2-HK
REC-PAS6-CBCR-PAS-HK
Fis9431DRAFT_3998
PCC9339DRAFT_00524
UYEDRAFT_06529
WP016873240
CACHE-HAMP-PAS4-GAF-CBCRPAS-HK
UYEDRAFT_00976
Stanieria cyanosphaera PCC
7437
Fischerella thermalis PCC
7521
Fischerella sp. PCC 9431
Fischerella sp. PCC 9339
Chlorogloeopsis fritschii PCC
9212
Chlorogloeopsis fritschii PCC
9212
UYKDRAFT_01008
1. Abbreviations for domains follow those of Table S1. Additional domains: TPR,
tetratricopeptide repeat domain; CACHE: Ca2+ channels, chemotaxis protein.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Table S3. Estimated extinction coefficients for far-red CBCRs in this study.1
Protein
15Z  (M-1 cm-1)
Anacy_4718g3 (intein-CBD)
40,800
Anacy_4718g3 (His tag)
44,400
Anacy_2551g3 (His tag)
49,200
Cyan7822_4053g2 (intein-CBD)
39,900
Sta7437_1656 (His tag)
43,600
1. All values are for PCB adducts. Comparison of values for different Anacy_4718g3 constructs
suggests an error range of ±10%. WP_016871037 was excluded due to our inability to obtain a
native 15Z spectrum in the absence of 15E bilin.
Rockwell et al., “A conserved cyanobacteriochrome lineage detecting far-red light”
Table S4. CCA properties for cyanobacteria hosting RcaE-type CBCRs.1
cyanobacterium
green/red CBCR
CCA type
Staniera cyanosphaera PCC 7437
Sta7437_2963
III
Synechococcus sp. PCC 7336
WP_017324438 (Syn7336_0672)
III
Xenococcus sp. PCC 7305
X7305_GR
III
(Xen7305DRAFT_00010580)
Leptolyngbya sp. JSC-1
JSC1_30280 (CYJSC1_DRAFT_30280)
III
Tolypothrix sp. PCC 76012
RcaE (fdiDRAFT24240)
III
Synechococcus sp. PCC 7335
S7335_5042g1 (S7335_5042)
III
1. Strains hosting the RcaE cluster identified in Fig. 2 are reported where information about CCA
is available. Both the protein tag used in Fig. 2 and the DOE-IMG locus tag are listed when they
are different. Information about strains in the Pasteur Culture Collection (PCC) is taken from the
PCC catalog (http://catalogue.crbip.pasteur.fr/crbip_catalogue/faces/recherche_catalogue.xhtml),
and information about Leptolyngbya sp. JSC-1 is taken from Ref. 102 in the main manuscript.
2. Also known as Fremyella diplosiphon.