Wellsway school science dept. Curriculum 2011 – AQA Additional Science -1- Biology workbook Wellsway school science dept. Curriculum 2011 – AQA Additional Science Biology workbook WORKBOOK 1 – CELLS & CELL TRANSPORT Cells and simple cell transport All living things are made up of cells. The structures of different types of cells are related to their functions. To get into or out of cells, dissolved substances have to cross the cell membranes. Candidates should use their skills, knowledge and understanding to: ■ relate the structure of different types of cells to their function. Subject content - Cells and cell structure a) Most human and animal cells have the following parts: ■ a nucleus, which controls the activities of the cell ■ cytoplasm, in which most of the chemical reactions take place ■ a cell membrane, which controls the passage of substances into and out of the cell ■ mitochondria, which are where most energy is released in respiration ■ ribosomes, which are where protein synthesis occurs. b) Plant and algal cells also have: a cell wall made of cellulose, which strengthens the cell. Plant cells often have: ■ chloroplasts, which absorb light energy to make food ■ a permanent vacuole filled with cell sap. c) A bacterial cell consists of cytoplasm and a membrane surrounded by a cell wall; the genes are not in a distinct nucleus. d) Yeast is a single-celled organism. Yeast cells have a nucleus, cytoplasm and a membrane surrounded by a cell wall. e) Cells may be specialised to carry out a particular function. 1 Revise with your teacher the basic structure & functions of animal & plant cells. Watch the video or PowerPoint on cells. Write the functions of these 8 structures: All cells – cell membrane encloses all cells; controls what enters & leaves All cells – cytoplasm site of all reactions of life All cells – nucleus controls activities of cell; contains chromosomes All cells – mitochondria releases energy for cell by aerobic respiration All cells - ribosomes site of protein synthesis Plant cells only – cell wall encloses plant cell, provides support to turgid cells Plant cells only – large vacuole filled with sap, provides support to turgid cells Plant cells only – chloroplasts absorb light energy so plant can photosynthesise Resources: Biology for You, page 17, Qs 2 and 3; page 40, Qs 1 and 2,. -2- Wellsway school science dept. 2 Curriculum 2011 – AQA Additional Science Biology workbook Prepare an onion epidermis (skin) slide using the worksheet/instructions provided: Safety – take care with glass slides and cover slips. Place them in the container provided at the end. Be careful with iodine, which stains hands, clothes, books, etc. Ensure no stain gets on your microscope. Be careful when carrying and using your microscope. 3 Look at your slide under your microscope (or use a Bio viewer and filmstrip). You should be able to see: Cell wall Nucleus Position of cell membrane Position of vacuole. Position of cytoplasm One common structure often found in plant cells is not present in onion skin. Write down which structure is missing, and suggest why this is (hint: onion bulbs grow underground). No chloroplasts – no sunlight available underground, so a waste of energy producing structures which cannot be used. 4 Draw 2-3 onion epidermis cells in detail below, and label your drawing. Cell wall vacuole Cell membrane Nucleus Position of cytoplasm -3- Position of Wellsway school science dept. 5 Biology workbook Observe some animal cells using a Microscope OR a Bio viewer OR a textbook. Draw 2-3 animal cells in detail below, and label your drawing. Cell membrane 6 Curriculum 2011 – AQA Additional Science Nucleus Cytoplasm Position of Mitochondria Observe photographs or drawings of typical bacterial cells. Draw and label a bacterial cell to highlight the key differences between it and an animal and plant cell. -4- Wellsway school science dept. 7 Curriculum 2011 – AQA Additional Science Biology workbook Observe photographs or drawings of typical yeast cells. Draw and label a yeast cell to highlight the key differences between it and an animal, plant and yeast cell. Yeast cells are much larger than bacteria (4 – 12 µm = 4/1000 – 12/1000 mm) – similar in size to a red blood cell. Fungal cells are similar to plant cells BUT lack chloroplasts & chlorophyll. 8 Read your textbook or support material on specialised cells. Draw 2 different animal cells and 2 different plant cells, and explain how they are specialised to carry out their functions. Specialised Animal & Plant Cells -5- Wellsway school science dept. Specialised Plant Cells Curriculum 2011 – AQA Additional Science Biology workbook See previous diagram. Dissolved substances a) Dissolved substances can move into and out of cells by diffusion. b) Diffusion is the spreading of the particles of a gas, or of any substance in solution, resulting in a net movement from a region where they are of a higher concentration to a region with a lower concentration. The greater the difference in concentration, the faster the rate of diffusion. c) Oxygen required for respiration passes through cell membranes by diffusion. 1 Watch the teacher demonstrations showing diffusion. Read your textbook or support material on diffusion. Write down a definition of diffusion: Diffusion is the random movement of particles (of a liquid or gas) from a region of high concentration to a region of low concentration. Write down some examples of diffusion of substances across membranes: (a) in animal cells. Oxygen into the blood; oxygen from blood to cells; glucose into cells. (b) in plant cells. CO2 into leaves; O2 out of leaves -6- Wellsway school science dept. 2 Curriculum 2011 – AQA Additional Science Biology workbook Make a list of the factors which can affect the rate of diffusion: Temperature (higher temperature = faster diffusion; particles have more kinetic energy) Concentration difference / gradient (greater conc difference = faster diffusion) Concentration of particles (higher concentration = faster diffusion) 3 Investigating the effect of temperature and concentration on the rate of diffusion. SAFETY: ammonia gas is an irritant. wear eye protection & avoid inhaling ammonia vapour. Apparatus: Apparatus: (per group): Glass diffusion tube UI paper Glass rod Ruler 10 mls Ammonia solution in a small beaker 5 ml syringe Stop watch 10 mls Distilled water in a small beaker Method: • Set up the apparatus as shown in the diagram. • Syringe 3 mls of ammonia solution onto some cotton wool and immediately push the cotton wool into the end of the glass tube. • Start the stop watch. • Record the time taken for the pieces of universal indicator paper to turn blue. • Repeat the procedure using warm ammonia solution, and (if you have time) dilute ammonia solution. Variables: Write down: (a) The independent variables (factors you are changing): Concentration of ammonia / temperature of ammonia (b) The dependent variable (factor you are measuring/finding out): Time taken for UI paper to turn blue (c) The main control variables (factors you will keep the same for fair testing): Volume of ammonia (3 mls) Size of cotton wool; how far cotton wool is pushed into the tube; size and spacing of pieces of UI paper. -7- Wellsway school science dept. Curriculum 2011 – AQA Additional Science Biology workbook Predictions: I think the fastest diffusion will take place with… Hot ammonia Because… The gas particles have more kinetic energy and are moving around faster I think the slowest diffusion will take place with… Dilute ammonia Because… There are less particles to diffuse, so they have less kinetic energy Carry out your investigation carefully, and record all your results in a neat clear table: Results: Typical answers – these can vary widely. Time taken for UI paper to turn blue (mins + secs.). UI paper no. 1 2 3 4 5 6 7 8 9 10 Full strength Ammonia 10 11 30 40 50 1:00 1:20 1:40 2:10 3:00 Hot Ammonia Dil. Ammonia 2 6 10 20 25 30 45 50 1:00 1:10 19 22 30 1:13 2:10 2:53 4:09 4:23 5:10 6:15 Conclusion: Write down: (a) what you have found out: Hot ammonia diffuses more quickly than cold. Dilute ammonia diffuses more slowly than concentrated ammonia. (b) whether your predictions were correct: (c) why you have obtained these results (i.e. a scientific explanation): Ammonia is an alkaline gas & diffuses quickly – from high to low concentration - onto the UI paper, turning it blue. Diffusion slows down the further the UI paper is away from the cotton wool. Hotter gas particles have more kinetic energy and are moving around faster, so they diffuse faster. In dilute ammonia there are fewer particles to diffuse, so they have less kinetic energy and diffuse more slowly. -8- Wellsway school science dept. Curriculum 2011 – AQA Additional Science Biology workbook Well done – you have finished the Cells & Cell Transport workbook! Useful web links & clips: General http://www.bbc.co.uk/schools/gcsebitesize/biology/cellprocesses Animal Cells: general structure http://clipbank/espresso/clipbank/servlet/link?macro=setresource&template=vid&resourceID=1 542&taxonomyNodeID=721 Animal cells: types & functions http://clipbank/espresso/clipbank/servlet/link?template=vid¯o=setResource&resourceID=1 543&taxonomyNodeID=721 Leaf & Plant Cell Structure http://clipbank/espresso/clipbank/servlet/link?template=vid¯o=setResource&resourceID=1 544&taxonomyNodeID=721 Plant cells: general structure http://clipbank/espresso/clipbank/servlet/link?macro=setresource&template=vid&resourceID=1 545&taxonomyNodeID=721 Plant cells: types & functions http://clipbank/espresso/clipbank/servlet/link?template=vid¯o=setResource&resourceID=1 546 Glossary (muddled – sort them out!) 1 Carbon dioxide 2 Cell membrane 3 Cell wall 4 Chloroplast 5 Chromosomes 6 Cytoplasm 7 Diffusion 8 Diffusion (concentration) gradient 9 Mitochondria 10 Nucleus 11 Oxygen 12 Ribosomes 13 Vacuole Jelly-like region in all cells, where reactions of life take place. Small green structure in some plant cells. Absorbs light for photosynthesis. Tiny threads inside the nucleus, which are composed of lengths of DNA called genes. These produce our inherited characteristics. Outer boundary of all cells. Controls what enters & leaves. Extra boundary around plant cells. Made of cellulose, for support. Gas released from respiration & removed through the cell membrane by diffusion. Small structures in all cells which release energy by aerobic respiration. Tiny structures in all cells where protein synthesis takes place. Relatively large structure in all cells which controls the cell’s activities. Contains chromosomes. Random movement of particle of gas or liquid from a higher concentration to a lower concentration. Sap-filled bag in plant cells which provides support to turgid (swollen) cells Difference in concentration of a substance which determines how quickly diffusion takes place. Gas required for respiration absorbed through the cell membrane by diffusion -9- 6 4 5 2 3 1 9 12 10 8 13 8 11 Wellsway school science dept. Curriculum 2011 – AQA Additional Science Additional Notes - 10 - Biology workbook Wellsway school science dept. Curriculum 2011 – AQA Additional Science Questions. a) Biology for You questions. - 11 - Biology workbook Wellsway school science dept. Curriculum 2011 – AQA Additional Science b) AQA Past paper questions. - 12 - Biology workbook Wellsway school science dept. Curriculum 2011 – AQA Additional Science Biology workbook 1 The diagram shows a cell from the lining of the lung. This cell is specialised to allow gases to pass through quickly. 1 (a) Use words from the list to label structures A, B and C. cell membrane chloroplast cytoplasm (3 marks) mitochondria nucleus 1 (b) (i) Which feature of this cell allows oxygen to pass through quickly? (1 mark) Put a tick next to your choice. It is thin. It has a large nucleus. It has many mitochondria. 1 (b) (ii) Complete the sentence by drawing a ring around the correct answer in the box. (1 mark) Oxygen passes through this cell by diffusion osmosis respiration (F PAPER - JUN 2008) 2 The diagram shows a group of muscle cells from the wall of the intestine. - 13 - Wellsway school science dept. Curriculum 2011 – AQA Additional Science Biology workbook (a) On the diagram, use words from the box to name the structures labelled A, B and C. cell membrane cell wall chloroplast cytoplasm nucleus (3 marks) (b) How are these muscle cells adapted to release a lot of energy? ....................................................................................................................................... ....................................................................................................................................... (2 marks) (F PAPER – JAN 2008) - 14 - Wellsway school science dept. Curriculum 2011 – AQA Additional Science - 15 - Biology workbook
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