EAS32
v 1.4
Getting started
Rev. D - 23 May 2003
Tel: +39 02 9231491
Fax: +39 02 9232703
E-Mail: [email protected]
Web: www.costech.net
© Costech International S.r.l.
Via Vittorio Veneto 8
20063 Cernusco s/Nav. Milan
Italy
List of Contents
1
2
3
4
5
6
Brief Description of the EAS32...................................................5
1.1
Hardware and Software Requirements .............................5
Installation..................................................................................7
2.1
Software Installation..........................................................7
2.2
Hardware Installation ........................................................7
2.2.1 The INT7 Card.......................................................7
2.2.2 The INT7 card and Windows NT............................8
2.2.3 Installation of the U-PAD Converter.......................9
2.3
Connecting the Chromatograph ........................................9
2.4
Configuring EAS32 .........................................................11
Program Structure and Operation ............................................13
3.1
Main Station Window ......................................................13
3.2
Channel Windows ...........................................................14
3.3
Chromatogram Window ..................................................15
3.4
Calibration Window .........................................................16
3.5
Ending The Program.......................................................17
First "Analysis" .........................................................................17
4.1
Running the Program ......................................................17
4.2
Signal monitoring ............................................................17
4.3
Measurement and evaluation parameters.......................17
4.4
Running an Analysis .......................................................18
4.5
Analysis Monitoring .........................................................18
4.6
Preparations for stopping an analysis .............................20
4.7
End of Analysis ...............................................................20
4.8
What name will a saved chromatogram bear? ................21
4.9
Displaying and evaluating chromatograms .....................21
4.10 Manual Chromatogram Modification or Repeated
Processing? ....................................................................21
4.11 Printing............................................................................22
4.12 Calibration.......................................................................22
4.12.1 Creating a calibration curve .................................23
4.12.2 Using the calibration curve...................................23
Conclusion ...............................................................................24
Troubleshooting .......................................................................25
6.1
Problems Occurring while Starting up the
Workstation .....................................................................25
6.1.1 If You are Using the INT7 PCI Card.....................25
6.1.2 If You are Using U-PAD.......................................27
List of Contents
7
6.1.3 If You are using the evaluation – Eval –
version or the full version without A/D
converter..............................................................28
6.2
Problems Occurring while Collecting Data ......................30
6.3
Problems Occurring while Installing the INT7
Card Driver......................................................................31
6.4
Problems Occurring while Installing the U-PAD
Driver ..............................................................................34
Index . ....................................................................................37
EAS32 - Getting started
Brief Description of the EAS32
1 Brief Description of the EAS32
The Elemental Analysis Software is an effective tool for collecting, processing
and evaluating data from any gas or liquid chromatograph. It consists of a four
channel internal or 2 channel external 24 bit integrating A/D converters (PC
boards) and the application program proper. In its maximum configuration the
station can measure up to four independent analyses at four detectors
simultaneously. The station supports automated co-operation with chromatographs
and autosamplers.
Since the station runs under the Windows operating system other, independent
applications may run simultaneously. Moreover, the Windows environment
enables a facile transfer of chromatographic results to other programs for further
modification and evaluation.
EAS32 automatically converts all data of the EAS17 Station.
1.1 Hardware and Software Requirements
• The EAS32 workstation can be used with any of the following operating
systems: Windows 98/Me, Windows NT/2000/XP, in any language version.
• Use this table to check the compatibility of your workstation:
EAS32
MS Windows 98/ME
INT7
MS Windows NT
INT7
MS Windows 2000/XP
INT7
U-PAD
U-PAD
• The computer requirements are first and foremost determined by your operating
system:
Under Windows 98/Me, with limited speed expectations, a computer
with a Pentium/200 with 32 MB of memory is sufficient.
-
For Windows NT system, a computer with a Pentium/450 with 64 MB of
memory.
-
Under Windows 2000/XP systems we recommend a computer with a
Pentium/750 with 128/256 MB of memory.
5
EAS32 - Getting started
Brief Description of the EAS32
The minimal screen resolution is 800x600 pixels and 256 colours; the
recommended resolution is 1024x768 and 64K colours (16 bit – High Colour).
• Verify that you have:
A free slot (or port) for your converter:
INT7,
- PCI slot
U-PAD
- USB port
-
A CD ROM drive for software installation
6
EAS32 - Getting started
Installation
2 Installation
Verify that the package is complete (packing list).
2.1 Software Installation
Installation of the program requires 10 to 30 MB of free hard disk space –
depending on the number of modules you want to install.
• Insert the CD into the drive.
• If the installation software does not start up on its own, select the file
INSTALL.EXE and run it.
• The software installation wizard will take you through the installation procedure,
including the creation of a EAS32 item in the Start - Program menu and an
EAS32 icon
on the Desktop.
2.2 Hardware Installation
The following chapters describe installation of the various devices that you can use
together with Elemental Analysis Software.
2.2.1
The INT7 Card
•
•
•
•
Turn off the PC
Insert the INT7 card into the PCI slot
Start up the PC
During the start-up, Windows will automatically recognize the new Plug and
Play device and will attempt to install a driver. The “Found New Hardware
wizard” will appear.
• Select: "Search for a suitable driver for my device."
• Select: "Specify a location"; here click the Browse button and select the path to
the CD drive from which you have installed the EAS32 program, then select
subdirectory INT7/WIN9X (or 2000). If you have installed the workstation before
inserting the card, you will also find an identical subdirectory in the main
directory of the workstation. The driver is in a file named CSWINT7.VXD
(CSWINT7.SYS under Windows NT/2000) and its information file is CSWINT7.INF.
• The rest of the installation will be carried out automatically.
7
EAS32 - Getting started
Installation
Fig. 1 The Found New Hardware Wizard
• Check whether the driver is correctly installed:
Windows
In the lower left corner of the screen, in the Start menu, select
Settings – Control Panel, and the System icon; select the
95/98/Me
Device Manager tab and look for the item Chromatography
Device - Acquisition Device Model 7.xx.
Windows
2000/XP
2.2.2
In the lower left corner of the screen, in the Start menu, select
Settings – Control Panel, and choose the System item; under
the Hardware tab click on the Device Manager button and in
the resulting list look for the item Chromatography Device Acquisition Device Model 7.xx.
The INT7 card and Windows NT
• For Windows NT, which is not a Plug and Play operating system, the entire
installation consists in copying the driver, an operation which the main EAS32
installation program will perform. This means that Windows NT does not detect
the inserted card and will not require a special driver. Should problems occur,
you will find detailed information in chapter 6 - Troubleshooting.
8
EAS32 - Getting started
2.2.3
Installation
Installation of the U-PAD Converter
• Connect the U-PAD with a cable to a USB port on the computer.
• After connecting the converter, Windows will recognize the new Plug and Play
device and will attempt to install a driver. The hardware installation wizard will
appear:
• Select: "Search for a suitable driver for my device."
• Select: "Specify a location"; here select the path to the CD drive from which you
have installed the EAS32 program, then the UPAD subdirectory. If you have
installed the workstation before connecting the device, you will also find an
identical subdirectory in the main directory of the workstation. The driver is in a
file named CSWUPAD.SYS and its information file is CSWUPAD.INF.
• The rest of the installation will be automatically carried out. In the lower left
corner of the screen, in the Start menu, select Settings – Control Panel;
selecting the System icon, you can verify that the Device Manager tab contains
a correctly installed "Universal Serial Bus Controllers" - "U-PAD v. 1.1"
item.
2.3 Connecting the Chromatograph
• When one of the A/D converters is used (INT7, U-PAD), the EAS32
workstation uses the following cables to communicate with the chromatograph:
Signal cables (labeled as tension signal on the image) are identified as
"1" through "4" depending on the number of channels; the cables are
normally delivered without connectors for connecting the chromatograph
(free wires only – red, white and ground).
Starting (marker) cables identified as "M1" through "M4" depending
on the number of channels; the starting cables are terminated on an
CINCH female connector. For each starting cable, there is one cable
adaptor included which is terminated on a button, and one cable adapter
with free wires allowing connection directly to a chromatograph or valve.
On the converter side, the cables are always terminated on a 37-pin
CANNON female connector.
9
EAS32 - Getting started
Installation
• Connect the cables according to the following schematic:
CAUTION! The connection of the 37-pin connector may not be
substituted among various other A/D converters. Only the INT7
card and the U-PAD use the same connection. A detailed
description of the connection of each connector can be found in
the Reference Guide, chapter Technical Specifications.
• Rules to Follow when Connecting the Signal Cables:
The signal inputs use a so-called symmetrical cable: + (red), - (white),
analogue ground (brass braiding).
The screening must be connected. This is because the braiding is not only
fulfilling a shielding function, but is mainly grounding the device.
Should there be a non-symmetrical output on the detector
(with only two terminals), the screening must be
connected to the white wire! None of the wires of a signal
cable should remain unconnected.
-
Try to make the connection on the output of the chromatograph detector
with the highest signal level – usually identified as INTEGRATOR (with
signal around 1V). The output identified as RECORDER uses only a
signal of around 10mV.
10
EAS32 - Getting started
Installation
• Connecting the Starting cables:
The start-up input reacts to a change in the TTL logical level or to
connection by any electrical contact (button, relay).
By default, the input reacts to a change from logical one to zero (=
connection of the contact device). This may be changed using the
command Method - Acquisition - Ext. Start in the Channel window.
2.4 Configuring EAS32
• Using the icon on the Desktop, start up the Elemental Analysis Software.
• In the System – Configuration menu, select the System Configuration dialog
window.
• Configure the number of channels in the No. of Channels field.
• From the right-hand-side column, double-click on a device driver that
corresponds to your configuration:
Fig. 2 The Main tab of System configuration window
Immediately after double-clicking, a configuration window will appear for the
corresponding card or device:
11
EAS32 - Getting started
Installation
Fig. 3 The INT7 board config window
Check whether everything is in order and whether the right number of channels is
displayed, and check the other settings if need be.
Under the tab of each one of the channels of the workstation, select the correct
channels of the A/D converters (Acquisition) eventually customize the name of
the Channel (Name).
Fig. 4 The Channel tab of System configuration window
12
EAS32 - Getting started
Program Structure and Operation
3 Program Structure and Operation
The EAS32 has a hierarchic structure. When run, the program display the Main
window with icons of chromatographs (detectors) attached to individual channels.
Clicking an icon of chromatograph and entering a User's name displays the
corresponding Channel window earmarked for measurement and evaluation by
means of the connected chromatograph.
The EAS32 is characterized by a channel - detector concept; this means
that all channels are independent and each channel is assigned to a
single detector at a given time. In case of multi-detector measurement,
e.g., with a gas chromatograph, related channels can be synchronized to
obtain analyses measured with more than one detector.
3.1 Main Station Window
In the main window the station configuration is set and access rights are
selected along with the basic directories for data storing and sharing among
other EAS32s in a computer network.
Detector
name
User Accounts
Configuration
Symbol of
opened
channel
Symbol of
closed
channel
User name
Fig. 5 The Main station window
or the command System - Configuration)
In station configuration (icon
you can set the number of displayed channels, their synchronization, the type
of controlled chromatographs, and select the displayed icons and detector
descriptions. Access rights (icon
or the command System – User
Accounts) serve for assigning names and passwords to individual users and
deciding on access to individual operations (e.g. access to files, authorisation to
modify calibration, integration parameters, etc.); each user may then save
customized station appearance.
13
EAS32 - Getting started
Program Structure and Operation
3.2 Channel Windows
Channel window serves for acquisition and evaluation of analysis from the
selected detector. You can display the window by clicking the symbol of the
corresponding chromatograph in the Main station window. Each Channel
window contains a StatusTable and a graphical scheme of analysis
processing. Depending on the number of selected channels one may display up
to four independent Channel windows, distinguished by the colours of lines
showing the analysis processing scheme, by the name of the connected detector
at the title bar, potentially also by the name of the project (directory) at the
status bar.
Detector name
Name of displayed
method
Status table
Chromatogram
name
Sample table
Sample description
Conditions of data
acquisition
Current method file
Chromatogram
display
Creation of
calibration curves
Print
Conditions of
analysis
Project name
(=subfolder)
Conditions of analysis
evaluation
Analysis monitoring
Fig. 6 The Channel window
By clicking an icon in the scheme (or choosing a command from the menu) you
display windows that activate the desired functions for the selected
chromatograph (detector). Each Channel window has its own independent
windows; thus, you may monitor measurement at more than one channel
simultaneously.
14
EAS32 - Getting started
Program Structure and Operation
When working with the so-called modal windows there is a certain
restriction, though. No other window is accessible while a modal
window is open. Modal windows include in particular windows for
file selection, parameter setup, etc.
3.3 Chromatogram Window
This is the fundamental window for displaying, modifying and evaluating
chromatograms. You can open it by clicking the
. Clicking the
icon you
can display list of already measured chromatograms.
Chromatogram selection
for display
Chromatogram
modification
Switch to channel or
calibration window
Results table
Work with several
chromatograms
Tabs of other
tables
Custom label
Setting of result
calculation type
Fig. 7 The Chromatogram window
The Result Table referring to the opened chromatogram is on the Results tab.
If not displayed automatically, click the label of the Results tab in the bottomleft corner of the window or invoke the command Results – Result Table.
Refer to Chapters 4.9 - 4.10 for a description of modifications and
chromatogram evaluation methods.
How can you magnify displayed chromatogram?
You can hide the small preview graph by command Display – Small
15
EAS32 - Getting started
Program Structure and Operation
Graph.
By double clicking near to the splitter (the cursor will change to ) you
can zoom the graph to fill the whole window.
maximises Chromatogram window.
Clicking the icon on window title
3.4 Calibration Window
In this window one creates, modifies and displays calibration curves. To open
icon. Chapter 4.12 on page 22 explains how to create and use a
it click the
simple calibration.
Calibration
file selection
Display
calibration
standard
Display global calibration table
and calibration standard
Switch to other
windows
Global
calibration
table
Display selected
compounds
Fig. 8 The Calibration window
16
Select
calibration
level
Select
calibration
type
Calibration standard
name
EAS32 - Getting started
First "Analysis"
3.5 Ending The Program
For ending program use the command System - Exit from Main station
window. Before ending you are notified of data acquisition process as well as
of the fact that some file has been modified and has not been saved yet.
4 First "Analysis"
By describing a model analysis we wish to show the essential practical
procedures of working with the EAS32. The description however does not
cover all possibilities how to set up the station. In this part we assume your
EAS32 has been already installed and connected to the chromatograph.
4.1 Running the Program
The ability to start measurement without protracted preparation and setup is a
distinct advantage of the EAS32 station.
Run the program by clicking the
icon on your desktop or choosing the Start
- Programs – EAS32. Then click appropriate chromatograph icon. The
Channel window will be displayed and you can already proceed with injection.
However let us explain some of the functions first.
4.2 Signal monitoring
Display the topical detector signal by clicking the
icon and check for drift
and noise (see the Fig. 10 on p.19). Then close the window, e.g., by
icon or
choosing the File - Exit command.
4.3 Measurement and evaluation parameters
Parameters that govern and describe the measurement, integration and
evaluation conditions are stored in the Method file. Some of the parameters
must be set prior to measurement - voltage range, Sampling rate,.. (any change
effected during analysis is blocked since it may corrupt the results).
When you put the mouse pointer over
. Click one of
it will turn into
the four icons to display the contents of the current Method file.
The Method is subdivided into following sections (see the Fig. 11 on p. 20):
17
EAS32 - Getting started
First "Analysis"
Measurement
-
section describing measurement conditions with the
possibility to set automatic length of analysis.
Acquisition
-
section with acquisition parameters - voltage range,
sample rate, …
Integration
-
section with integration parameters, integration table, …
Calculation
-
section with the type of calibration calculations
You can select another Method file by File – Open Method… command or by
icon.
4.4 Running an Analysis
Click the
icon and fill in the Sample Table.
Fig. 9 The Sample Table window
Start the analysis by invoking the command Run (
).
The inscription RUNNING will appear in the row below the
Channel window and the current retention time above it.
4.5 Analysis Monitoring
By clicking the
icon you may start monitoring the analysis.
18
icon in the
EAS32 - Getting started
First "Analysis"
Fig. 10 The Data Acquisition window
To zoom in on a part of the chromatogram hold down the left mouse button,
draw the mouse to select an area and release the mouse button. The original
size may be restored by choosing the icon
or double-clicking with the left
mouse button within the area of the graph.
You can control the data acquisition directly from this window by means of the
icons
Run,
Abort and
Stop.
An analysis in progress can be compared with an already measured
chromatogram, say a solvent, a calibration chromatogram, etc.
• Invoke the command File – Set Background Chromatogram… to select
the chromatogram to be displayed in the background of the Data
Acquisition window.
The background chromatogram will be displayed in grey colour so that it can’t
be mistaken for signal.
The background chromatogram will be displayed only after an analysis is run.
19
EAS32 - Getting started
First "Analysis"
4.6 Preparations for stopping an analysis
Prior to stopping an analysis it is advisable to check the integration parameters
icon or by Method – Integration command.
by clicking the
Fig. 11 The Method Setup - Integration window
Peak Width and Threshold are the most important parameters. They can be
described in a simplified manner as follows:
• Peak Width directs all peaks of width substantially narrower than the preset value to be ignored.
• Threshold specifies the admissible noise level by allowing only peaks at
least twice as high as the pre-set value to be detected.
The actual situation is somewhat more complicated since interplay of both
parameters and the peak shape is also important.
4.7 End of Analysis
To terminate a running analysis choose Stop from the Analysis window or
click the icon
in the Data Acquisition window; an analysis stops automatically once the time entered in the parameter Enable Autostop in the
20
EAS32 - Getting started
First "Analysis"
Method - Measurement window has passed. At that moment the collected raw
data are compressed, integrated, evaluated and saved.
4.8 What name will a saved chromatogram bear?
The column File name in the Sample Table window (Fig. 9 on page 18)
decides on the chromatogram name. Upon analysis completion you will be
notified if a file of identical name already exists. Here you can use the tool for
automatic naming of chromatograms e.g. sequential numbers by means of %n
or inserting of the date by means of %d etc.
4.9 Displaying and evaluating chromatograms
The completed analysis will be automatically displayed in the Chromatogram
window. If it is not displayed automatically, you might have clicked the symbol
at the
icon, thus changing it to .
icon. You can open
You may also open this window later by clicking the
icon. As in the signal monitoring window
the required chromatogram by
you may zoom in on any part of the chromatogram by holding down the left
mouse button, drawing, and releasing the button when the selected cut is
satisfactory.
4.10 Manual Chromatogram Modification or Repeated
Processing?
If the proposed baseline is deemed unsatisfactory, you may either manually
"tune up" the chromatogram, or change the integration parameters and
reprocess the whole chromatogram anew.
To manually "tune up" the chromatogram use the icons from the two sets of
instruments located in the bottom corners or the corresponding commands from
the submenu Chromatogram - Baseline or Peak. The effected changes will be
stored in the Integration and may be further modified or cancelled anytime.
To whole chromatogram reprocess open the Integration tab and change any
item from section Parameter. The reprocessing will be carried out
automatically. You can open the tab anytime by Method – Integration
command.
21
EAS32 - Getting started
First "Analysis"
4.11 Printing
To facilitate reporting of results all major windows contain the icons
and
,
.
icon in the Chromatogram window.
1.
Click the
2.
Select and configure a printer by Printer command in the upper part
of the displayed Report Setup window.
3.
By choosing Open select appropriate report style file.
A report style is in effect a format that specifies which information will be
printed and how. Information is divided into nine parts corresponding to the
nine displayed tabs. On each tab you can set more detailed setting of the
relevant part. The whole part may be included in the printout by checking the
Print item in the upper left corner of the tab or setting the item check in front
of the tab by double-clicking with the left mouse button.
By means of
icon .
icon you can display print preview; printing itself is done by
Different print styles are pre-set (and can be changed) in individual
parts of the station, so that the items relevant for the current window
in the program are printed as required.
4.12 Calibration
Creation and use of calibration curves comprises a substantial part of the
EAS32. Calibration curves created by calibration standards are stored in
separate calibration files. Each calibration file may contain practically
unlimited numbers of curves calibrated at up to twenty concentration levels.
Collecting calibrated results consists in creating calibration curves, storing
them in a calibration file, assigning the calibration file to a chromatogram, and
prescribing the required calculation method.
22
EAS32 - Getting started
4.12.1
First "Analysis"
Creating a calibration curve
Click the
icon or use the Window – Calibration command. In open
icon or File – New to create a new
calibration file. To open a chromatogram of a calibration standard click the
icon (red icon would open calibration file) or use File – Open
yellow
Standard command.
Calibration window use the
icon the available characteristics of all peaks are
By clicking the
automatically transferred to the calibration table, where you can add names
of compounds (the table cells are editable after double-click with left mouse
button).
Known weights of each compound can be filled in the column Weight
under the number of current calibration level. If you followed the above
instructions correctly a straight line representing a single-point calibration
curve should appear after clicking on the tab with the name of required
compound.
Finally save all the created calibration curves to file under selected name by
File - Save command from the menu.
The station allows for multilevel calibration and multiple recalibrations by
means of a selected algorithm. All this either automatically or by manual
selection of individual compounds including interactive filling in or
checking all data items. For all compounds you may also select the shape of
the calibration curve, the response type, the size of identification windows,
and improved peak identification.
4.12.2
Using the calibration curve
The simplest method how to check correctness of the established calibration
is to apply it to a chromatogram used to create it in the first place. Switch to
Chromatogram window (e.g. by
icon), display the window for
icon. Because of the calibration standards
chromatogram selection by
icon
are stored in CALIB subdirectory, select this directory first (e.g. by
in the upper right corner of Open Chromatogram window). Select the
chromatogram used for creation of calibration curves.
23
EAS32 - Getting started
Conclusion
Now set the calculation method:
1.
Display the Results tab.
2.
In its right part select your calibration file by command Set.
3.
Choose ESTD value in Calculation item.
Known weights including per cent content and name should appear at all peaks for
which calibration curves have been identified from coincidence of retention times.
Zero weights will be displayed for peaks to which no calibration compound applies
according to retention time. Identified peaks for which however no calibration
curve exists will be marked Free in the Peak Type column.
It follows from the above that calibration curves are stored neither with
the chromatogram nor with the method; instead, the chromatogram (or
rather the assigned method) contains a reference to the corresponding
calibration file. Other programs usually do not use this approach. Its
advantage consists in easy recalibration and easy changes in case a
calibration file applies to several chromatograms. A certain drawback is
the necessity to save the calibration file under a different name in the
event recent changes must not affect already evaluated chromatograms
(archiving original results).
5 Conclusion
We would be grateful for all comments, queries and inspiring ideas concerning the
EAS32 itself and this material. The current level of the program has been achieved
primarily through intensive communication with existing and potential users.
Thank You.
24
EAS32 - Getting started
Troubleshooting
6 Troubleshooting
6.1 Problems Occurring while Starting up the Workstation
DEMO - MISSING HW and message "Can't find integration card
or hardlock key"
6.1.1
If You are Using the INT7 PCI Card
Check in the System Configuration window to see whether the item INT7 A/D
Card driver appears on both lists. You can invoke the window using the
command System – Configuration from the Main window of the workstation.
a)
Item INT7 A/E Card driver is missing, but only in the list on the
right-hand side of the System Configuration window:
Try to add the item to the right by double-clicking on the list on the left-hand side:
-
-
b)
If an error message reading “Can't create detector” appears when you do this,
the registered file CSWINT7.DLL is probably incompatible with the current
version of EAS32. Details on installed registered files may be found in the file
SYSTEMINFO.TXT or by selecting the Help – About command in section
System Files. Repeat installation of the workstation.
If an error message appears reading “Error on Setup”, the driver of the card is
probably incorrectly installed. See below for a more specific description of the
driver control. Installation of the driver is not part of the installation of the
EAS32. Install the driver according to the installation instructions.
Item INT7 A/D Card driver does not appear in any of the lists of the
System Configuration window:
File CSWINT7.DLL is probably missing from the EAS32 directory, or this file is not
registered or is registered in a directory, which no longer exists. Repeat installation
of the workstation.
c)
Item INT7 A/D Card driver appears in both lists of the System
Configuration window:
Try to invoke the INT7 board config dialog window by double-clicking on the
item in the list on the right-hand side. If the dialog window does not appear, the
driver is probably incorrectly installed. Installation of the driver is not part of the
25
EAS32 - Getting started
Troubleshooting
installation of the EAS32. Install the driver according to the installation
instructions.
Checking whether the driver is correctly installed:
Windows NT:
This version of Windows is not a Plug and Play system.
Therefore, no special driver installation is required. You
probably got a
"Cannot find driver file
.…CSWINT7.SYS" error message.
Check whether the SYSTEM32/DRIVERS subdirectory of
Windows contains that file and, if need be, copy the file
from the INT7/NT subdirectory of the installation CD or
from the main directory of the EAS32.
Windows
95/98/Me:
In the lower left corner of the screen, in the Start menu,
select Settings – Control Panel and the System icon,
and look up the Device Manager tab for the item
“Chromatography Device - Acquisition Device Model
7.xx”.
Windows
2000/XP:
In the lower left corner of the screen, in the Start menu,
select Settings – Control Panel and the System icon,
and under the Hardware tab click on the Device
Manager button and look up the resulting list for the
item “Chromatography Device - Acquisition Device
Model 7.xx”.
Error status of the driver:
-
-
-
If item “Chromatography Device – Acquisition Device Model 7.xx” does
not appear, install this driver – see further information in chapter 2.2.1 The INT7 Card.
If the item does appear, but has a symbol with an exclamation mark or
question mark, try to reinstall the driver – see further information in
chapter 6.3 - Problems Occurring while Installing the INT7 Card
Driver.
If the item does appear, but has a symbol with a cross, double-click on it
to invoke the General tab and activate the driver by clicking on the
Enable this device button.
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EAS32 - Getting started
-
6.1.2
Troubleshooting
If the item does appear and there are no error symbols around it, you
probably reinstalled a driver for a different type of Windows operating
system, thereby replacing the correct driver. See more details in article
Technical details on the INT7 card driver on page 33.
If You are Using U-PAD
Check whether the System Configuration window contains the item USB
Acquisition driver in both lists. You may invoke the window using the command
System – Configuration in the Main window of the workstation.
a)
Item USB Acquisition driver appears only on the left-hand side of
the System Configuration window:
-
If an error message appears reading “Can't create detector”, the registered file
is probably incompatible with the current version of EAS32.
You may find more details on installed and registered files in the
SYSTEMINFO.TXT file, or using the command Help – About in section System
Files. Repeat installation of the workstation.
If an error message appears reading “Cannot load device driver CSWUSB”,
the UPAD is not connected to the computer or the driver is probably
incorrectly installed. Installation of the driver is not part of the installation of
the EAS32. Install the driver according the installation instructions.
CSWUPAD.DLL
-
b)
Carry out a verification of the driver, as follows
Windows
NT
Windows NT does not support USB devices – it is not
possible to use the EAS32 with U-PAD!
Windows
95/98/Me
In the lower left corner of the screen, in the Start menu,
select Settings – Control Panel and the System icon, and
look up the Device Manager tab for item "Universal Serial
Bus Controllers" - "U-PAD v. 1.1".
Windows
2000/XP
In the lower left corner of the screen, in the Start menu,
select Settings – Control Panel and the System icon, and
under the Hardware tab click on the Device Manager
button and look up the resulting list for item "Universal
Serial Bus Controllers" - "U-PAD v. 1.1".
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EAS32 - Getting started
Troubleshooting
Error status of the driver:
-
-
-
c)
If the item "Universal Serial Bus Controllers" - "U-PAD v. 1.1" does not
appear, install this driver – see chapter 2.2.3 - Installation of the U-PAD
Converter.
If the item does appear, but has a symbol with an exclamation mark or
question mark, try to reinstall the driver – see further information in
chapter 6.4 - Problems Occurring while Installing the U-PAD Driver.
If the item does appear, but has a symbol with a cross, double-click on the
icon to invoke section General and activate the driver by double-clicking
on the Enable device button.
Item USB Acquisition driver does not appear in either one of the
lists of the System Configuration window:
File CSWUPAD.DLL is probably missing from the EAS32 directory, or this file is not
registered or is registered in a directory, which no longer exists.
6.1.3
If You are using the evaluation – Eval – version or the
full version without A/D converter
In either case the Sentinel protective key is needed, either on a parallel or on a
USB port and its driver.
1. By selecting the Add or remove programs icon in the Control Panel
window, check whether the item Sentinel System Driver appears on the
list.
-
If it doesn’t, install the system driver:
Under Windows 95/98/Me by using the SENTW9X.EXE program in the
SENTINEL subdirectory of the EAS32.
Under Windows NT/2000/XP by using the SETUPX86.EXE program in the
SENTINEL subdirectory of the EAS32.
2. Check whether you have correctly connected the protection key:
a)
-
For a key connected to a parallel port:
Try to print on the connected printer.
Check whether the printing cable is not too long (>3m).
Disconnect the printer and cable and connect the key directly to the port
of the computer.
28
EAS32 - Getting started
b)
-
Troubleshooting
For a USB key, check the following:
A USB key may not be used under Win NT
See whether the USB port is working (e.g. try to connect a different
device,...)
See whether the HW driver is installed. In this case, the green LED on
the key should be lit. Check the status of the driver:
Windows
In the Control Panel select the System icon, access the
Device Manager tab and look for the Universal Serial
95/98/Me
Bus Controllers icon there should be an item named
“Rainbow Security Device”.
Windows
2000/XP
In the Control Panel select the System icon, under the
Hardware tab click on the Device Manager button: under
the Universal Serial Bus Controllers icon there should
be an item named Rainbow Security Device.
DEMO - WRONG S/N
The serial numbers of the workstation and of the A/D card or protective key do not
match. Use command Help – About in the Main window of the workstation to
check the serial number of the station under the S/N tab.
-
-
-
A serial number of the card or of the protective key of form XX – YYYYY
can be found using the Help – About command at the bottom of the System
Files section under line S/N.
The serial number of the workstation is of type XX – YYYYY +ZZ/NNN.
The significant part, which must match the number of the card is XX-YYYYY
(the initial zeroes are not significant), ZZ defines the driver modules allowed
and NN defines the number of measuring channels.
The number of the card can be found both written on the sticker label on the
inner side of the connector and in section System Files of the About window.
If you see the message “Can't Read”, you probably entered the user code
incorrectly. The 15-digit user code can be found on the installation CD or on
the attached CD package inside one of the guides. If necessary, contact the
manufacturer or your dealer to request this code – you will need to give the
serial number of the workstation. Verify the code and correct it if needed by
using the Help – User Code command in the Main window of the
workstation. The size of the characters does not matter. Be careful not to mix
up “I” with “1” on keyboard.
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EAS32 - Getting started
Troubleshooting
DEMO - MISSING EAS.SNO
File EAS.SNO is missing from the EAS32 directory is damaged or is inaccessible in
the main directory of the station, in which the user code of the workstation is kept.
Copy this file from the installation CD from the DEMO subdirectory. After starting
up the workstation, enter the correct serial number of the station using the Help –
User Code command.
DEMO
If you only see the Demo caption without any further description, you probably
did not enter the user code of the workstation during installation. Use command
Help – User Code to enter the serial number of the station, which you will find on
the installation CD or on the attached CD package inside one of the guides; if
necessary, contact the manufacturer or your dealer to request this code – you will
need to give the serial number of the workstation.
6.2 Problems Occurring while Collecting Data
Grey icon
with inscription „Disabled“ and command Monitor
- Data Acquisition is not working
Other signs of this error status: section Method – Acquisition is missing, the
Method – Acquisition command is not working, commands Run, Stop, Abort in
the Sample table window is not working. Some possible causes are:
1. No detector is attributed to the channel:
-
Close all channels and select the System – Configuration command in
the main window of the station to check under the tab of the
corresponding channel – Channel x: Under the Acquisition tab, select the
correct detector connected to the A/D card you are using. If your A/D
card is missing from the list, first go to the Main section and configure the
A/D card driver and then repeat the procedure.
30
EAS32 - Getting started
Troubleshooting
2. The licence you purchased allows for data collection from a lesser number
of channels:
-
Close all channels and, using the System – Configuration command in
the Main window of the workstation, look up the section of the relevant
channel – Channel x. If the Acquisition item is shaded grey, measuring is
not allowed on this channel.
Check your S/N serial number, e.g. using the Help – About command in
the Main window of the workstation. The number is of type: XX-YYYYY
+ ZZ/NNN.
3. You are using an evaluation version, which does not allow measuring
chromatograms.
-
Check whether the Main window contains the label Eval.
The Data Acquisition window
Simulated
contains a label reading
The corresponding channel will only display a simulated curve (from the
CHANNX.DTA file) because it has been assigned the so-called Demo driver. Close
all channels and, using the System – Configuration command in the Main
window, look up the tab of the relevant channel – Channel x: In the Acquisition
line, change Detector x on DEMO driver for the correct detector as is connected
to A/D card you are using. If you only see Demo detectors in the list, access the
Main tab to configure the driver of the A/D card, then get back to the Channel x
tab and configure the Acquisition line to the detector of the correct A/D card.
6.3 Problems Occurring while Installing the INT7 Card Driver
Installation of the driver itself
Installation of the INT7 card driver is not part of the installation of
the EAS32!
Windows NT is not a Plug and Play operating system: therefore it
does not detect the INT7 card, and installation of the driver is not
necessary.
For the other operating systems we recommend the following procedure:
1.
Install the EAS32.
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EAS32 - Getting started
Troubleshooting
2.
Switch off the computer, insert the INT7 card and start up the computer.
3.
As the computer starts up, the Windows operating system should
automatically recognize the new Plug and Play device and attempt to
install a driver. The new hardware installation wizard will appear:
a) Select: "Search for the best driver for your device."
b) Select: "Specify a location", here select the path of the CD drive
from which you have installed the EAS32 program, then select
subdirectory INT7/WIN9X (or 2000). If you have installed the
workstation before inserting the card, you will also find an identical
subdirectory in the main directory of the workstation.
c) The rest of the installation will be carried out automatically. Check
whether the driver is correctly installed:
Windows In the lower left corner of the screen, in the Start menu,
95/98/Me select Settings – Control Panel, and the System icon; under
the Device Manager tab look for the item “Chromatography
Device – Acquisition Device Model 7.xx”.
Windows In the lower left corner of the screen, in the Start menu,
2000/XP select Settings – Control Panel, and choose the System
item; under the Hardware tab, click on the Device Manager
button and look up the resulting list for the item
“Chromatography Device - Acquisition Device Model 7.xx.”
Windows does not recognize the card automatically
Try to find the card manually: in the lower left corner of the screen, in the Start
menu, select Settings – Control Panel, and choose item Add new hardware.
• If this succeeds in finding the card, the procedure to follow is the same as in
automatic detection.
• If the card is not found:
Restart the computer and see on the introduction black-and-white screen
whether you can find, in the PnP device section, the following line: in
column Vendor (ID) – value DAAE, and in column Product (ID) – value
DA70.
Otherwise, try to move the card to another PCI slot, and to temporarily
remove other cards if necessary.
If possible, try to repeat the procedure on a different computer.
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EAS32 - Getting started
-
Troubleshooting
Ask your dealer to replace the card.
Error symbol with “!“ or “?“ next to item Chromatography
Device - Acquisition Device Model 7...
• Double-click on the item to invoke the properties of the driver, and find out the
cause of the problem in the Device status field.
• Under the Resources tab, look for possible conflicts with other devices.
• In the Driver section, try to update the driver.
• Try to reinstall the driver:
Click on the Remove button to remove the driver.
Using the Add new hardware command in the Control Panels, try to
repeat the installation of the driver.
Technical details on the INT7 card driver
PCI cards may share interrupts. This means that several cards in the PC may be set
to the same interrupt.
The INT7 card driver is located in subdirectory INT7/WIN9X (or 2000 or NT) either
on the installation CD or, after installation of the workstation, in the main directory
of the workstation. There are three main drivers, depending on the type of
Windows system used:
Windows 95/98/Me:
- an information file, CSWINT7.INF (after installation, the file is kept in the
Windows subdirectory named INF\OTHER\ as DATAAPEX LTD.CSWINT7.INF).
- the driver as such, CSWINT7.VXD (after installation, the file is copied to the
Windows subdirectory named SYSTEM).
Windows 2000/XP:
- an information file, CSWINT7.INF (after installation, the file is kept in the
Windows subdirectory named INF\ as OEMX.INF, where X is a number
attributed in ascending order that depends on the number of OEM drivers
already installed).
- the driver as such, CSWINT7.SYS (after installation, the file is copied to the
Windows subdirectory named SYSTEM32/DRIVERS).
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EAS32 - Getting started
Troubleshooting
Windows NT:
-
the driver, CSWINT7.SYS (after installation, the file is copied to the Windows
subdirectory named SYSTEM32/DRIVERS).
6.4 Problems Occurring while Installing the U-PAD Driver
Installation of the driver itself
Installation of the U-PAD device driver is not part of the
installation of the EAS32!
Windows NT does not support the USB port: therefore, the UPAD device may not be used under Windows NT.
For the other operating systems, we recommend the following procedure:
• Install the EAS32.
• Using a USB cable, connect the U-PAD to any USB port on the computer.
• The Windows operating system should automatically recognize the new Plug
and Play device and attempt to install a driver. The hardware installation wizard
will appear:
Select: "Search for the best driver for your device."
Select: "Specify a location", here select the path to the UPAD subdirectory
in the Main directory of the EAS32.
The rest of the installation will be carried out automatically. Check
whether the driver is correctly installed:
Windows In the lower left corner of the screen, in the Start menu,
95/98/Me select Settings – Control Panel, and the System icon; under
the Device Manager tab, look for item "Universal Serial Bus
Controllers" - "U-PAD v. 1.1".
Windows In the Start menu, select Settings – Control Panel, and the
2000/XP System icon; under the Hardware tab click on the Device
Manager button and look up the resulting list for the item
"Universal Serial Bus Controllers" - "U-PAD v. 1.1".
34
EAS32 - Getting started
Troubleshooting
Windows does not recognize the U-PAD device automatically
Try to find the device manually: in the lower left corner of the screen, in the Start
menu, select Settings – Control Panel, and select the Add new hardware icon.
• If this succeeds in finding the U-PAD, the procedure to follow is the same as in
automatic detection.
• If the U-PAD is not found:
Restart the computer.
Check the cable.
As a last resort, try to repeat the procedure on a different computer.
Ask your dealer to replace the U-PAD device.
Error symbol with “!“ or “?“ next to "Universal Serial Bus
Controllers" - "U-PAD v. ..."
• Double-click on the item to invoke the properties of the driver, and find out the
cause of the problem in the Status line.
• Under the Resources tab, look for possible conflicts with other devices.
• In the Driver section, try to update the driver.
• Try to reinstall the driver:
Click on the Remove button to remove the driver.
Select the Add new hardware icon in the Control Panel window and try
to repeat the installation of the driver.
Technical details on the U-PAD driver
The driver of the U-PAD device is located in the UPAD subdirectory on the
installation CD or, after installation of the workstation, in the main directory of the
workstation:
Windows 95/98/Me:
-
an information file, CSWUPAD.INF (after installation, the file is kept in the
Windows subdirectory named INF\OTHER\ as DATAAPEX LTD.CSWUPAD.INF).
the driver as such, CSWUPAD.SYS (after installation, the file is copied to the
Windows subdirectory named SYSTEM).
35
EAS32 - Getting started
Troubleshooting
Windows 2000/XP:
-
-
an information file, CSWUPAD.INF (after installation, the file is kept in the
Windows subdirectory named INF\ as OEMX.INF, where X is a number
attributed in ascending order that depends on the number of OEM drivers
already installed).
the driver as such, CSWUPAD.SYS (after installation, the file is copied to the
Windows subdirectory named SYSTEM32/DRIVERS).
36
EAS32 - Getting started
Index .
7 Index .
-
A
All Data, 19
Analysis, 17, 18, 20
- Ending, 20
- Monitoring, 18
- Preparations for stopping, 20
- Running, 18
CSWUPAD.DLL, 27, 28
CSWUPAD.INF, 9, 35, 36
CSWUPAD.SYS, 9, 35, 36
OEMX.INF, 36
SENTW9X.EXE, 28
SETUPX86.EXE, 28
SYSTEMINFO.TXT, 25, 27
H
C
Hardlock, 28
Hardware Requirements, 5
Calibration Curve
- Creating, 23
- Using, 23
Compatibility, 5
Creating a Calibration Curve, 23
Ch
Channel, 14
Chromatogram, 15, 21, 22
- Displaying, 21
- Manual Modification, 21
- Name, 21
- Reintegration, 21
D
Data Acquisition, 18
Demo
- Missing EAS.SNO, 30
- Missing HW, 25
Description, 5
Displaying of Chromatogram, 21
I
Installation
- Hardware, 7
- INT7, 7
- Software, 7
- U-PAD, 9
INT7
- technical details, 33
- troubleshooting, 31
E
EAS32
- Brief Description, 5
- Running the Program, 17
Enable Autostop, 20
End, 20
End of Analysis, 20
Error on Setup, 25
M
Manual Chromatogram
Modification, 21
Measurement
- Evaluation, 17
- Parameters, 17
Method - Integration, 20
F
Files
- CSWINT7.DLL, 25
- CSWINT7.SYS, 26
37
EAS32 - Getting started
Index .
Modal Windows, 14
Monitoring
- Signal, 17
Monitoring of Analysis, 18
Starting cables, 9, 11
Status Table, 14
Structure of program, 13
T
N
Threshold, 20
Name of Chromatogram, 21
U
O
U-PAD
- technical details, 35
- troubleshooting, 34
Using the calibration curve, 23
Open, 22
P
Parameters, 17
Peak Width, 20
Preparations for stopping, 20
Printing, 22
Program structure, 13
W
Window
- Calibration, 16
- Data Acquisition, 18
- Found New Hardware Wizard, 8
- Channel, 14
- INT7 board config, 12
- Main, 13
- Method Setup - Integration, 20
- modal, 14
- Single Analysis, 18
- System configuration - Channel,
12
- System configuration - Main, 11
Windows NT
- INT7, 8
R
Report Setup, 22
Reprocess, 21
Running an Analysis, 18
Running the Program, 17
S
Sentinel System Driver, 28
Signal Cables, 10
Signal Monitoring, 17
Single Analysis, 18
Software Requirements, 5
Start, 17
38