The interleukin-13 receptor α2 chain: an essential
component for binding and internalization but not for
interleukin-13–induced signal transduction through the
STAT6 pathway
by Koji Kawakami, Jun Taguchi, Takashi Murata, and Raj K. Puri
Blood
Volume 97(9):2673-2679
May 1, 2001
©2001 by American Society of Hematology
125I–IL-13 binding to CHO-K1 and T98G cells transfected with the IL-13Rα2, IL-13Rα1, IL-4Rα,
and γc chains.cDNA for various receptor chains (6 μg/chain) was transfected into cells (1 × 106)
by using GenePORTER reagent for 48 hours.
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology
125I–IL-13 binding to T98G cells transfected with IL-13Rα1 or IL-13Rα2 chains.cDNA for receptor
chains (6 μg/chain) was transfected into T98G cells (1 × 106) by using GenePORTER reagent for
48 hours.
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology
Internalization of 125I–IL-13 in CHO-K1 and T98G cells.CHO-K1 (A) and T98G (B) cells were
incubated with 0.5 nmol125I–IL-13 at 4°C for 2 hours.
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology
Cytotoxicity of IL-13 toxin and IL-4 toxin to CHO-K1 cells transfected with various chains of IL13R.CHO-K1 cells were transfected with various receptor chains and then IL-13 toxin– or IL-4
toxin–mediated cytotoxicity was determined by protein synthesis inh...
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology
125I–IL-13 binding and cytotoxicity of IL-13 toxin to CHO-K1 cells transfected with IL-13Rα2
deletion mutants.Schematic representation of the wild-type and mutant IL-13Rα2 chains.
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology
Modulation of STAT6 activation by IL-13 and the IL-13Rα2 chain.CHO-K1 cells were transfected
with various receptor chains and incubated with IL-13 for 10 minutes, solubilized with cold
whole-cell extraction buffer, and 50 μg sample protein was incubated for...
Koji Kawakami et al. Blood 2001;97:2673-2679
©2001 by American Society of Hematology