Week 1 questions for article #1:
Set 1:
What is the motivation of the author in this paper?
Is it confirmed that LGR5+ is the only stem cell population in the gut? How was it
selected?
What component of Wnt signaling leads to Lgr5+ quiescent? What is the pathway?
Set 2:
How intestine stem cell potency achieved? What is their cellular composition?
To what cell type does LGR5+ cells differentiate? How was it proven experimentally?
How does it similar/ different than hESC?
Set 3:
What is the importance of studying EEC? In this paper, were proper controls for EEC
fate were used? What are the key factors to establish EEC differentiation?
Set 4:
What are some caveats in single cell analysis?
What are some examples for present/
future therapeutics with stem cell organoids?
List 2/3 critics concern about this
paper.
How would you continue this research? What is the next scientific question?
Week 1 questions for article #2:
1. What are the differences between population level and single cell level
measurements of cell cycle time (CCT)?
2. How do the authors reconcile the multimodal cell cycle time at the single cell level
and the uniform cell cycle time at the population level through simulations? What are the
differences between the models proposed by authors?
3. What are the differences between progenitor cells and terminally differentiated cells
in their circadian rhythms?
4. How do the authors experimentally prove that WNT but not Wee1 is responsible for
the coupling between circadian clock and cell cycle?