| Directed Differentiation of iPS Cells into Systems to |
|Model CF Lung Disease |
Tentative Schedule
Day 1- iPS generation and maintenance of pluripotent cells
Time
Topic
8:30-9:00
Registration/Breakfast (provided)
9:00-10:00
Welcome presentation by Dr. Christine Bear
10:00-11:00
Lecture 1: Feeder-Free Culture Systems for Human iPS Cells by
Stem Cell Technologies
11:00-11:15
Break
11:15-12:00
Practical 1: Evaluate the Quality of hES and iPS Cell Cultures
12:00-1:00
Lunch (Provided)
1:00-2:00
Lecture 2: Generation of the iPS cells by Center for
Commercialization of Regenerative Medicine (CCRM)
2:00-2:15
Break
2:15-3:15
Practical 2: Passage of PSC for Long-Term Maintenance and
single cell adaptation
3:15-3:30
Coffee Break (Provided)
3:30-3:45
Practical 3: Preparation of transwells for cell seeding
3:45-4:45
Practical 4: Preparation of cells for definitive endoderm
differentiation
4:45-5:00
Wrap up session
6:00-9:00
Dinner (provided) and social activity—TBA
Day 2- Definitive endoderm differentiation and early stages of lung
differentiation
Time
Topic
8:00-9:00
Breakfast (Provided)
9-10
Lecture 3: Endoderm Differentiation of Human Pluripotent Stem
Cells in Defined Culture Environments by Stem Cell Technologies
10-10:15
Break
10:15-11
Practical 5: Differentiation of PSC towards definitive endoderm
11-11:45
Practical 6: Seeding DE cells onto transwells
11:45-12:45
Lunch (Provided) & Flow cytometry presentation by Miltenyl
Biotec
12:45-2:15
Practical 7: Characterization of DE differentiation by flow
cytometry by Miltenyl Biotec
2:15-2:30
Break
2:30-3:30
3:30-3:45
3:45-4:15
4:15-4:45
Lecture 4: Directed differentiation of human pluripotent stem
cells into mature airway epithelia by Dr. Wong
Coffee Break (Provided)
Practical 8: Observation and discussion of differentiation stage 2;
specification of lung endoderm
Practical 9: Observe and maintenance of cultures prepared on
Day 1
4:45-5:00
Wrap up session
Day 3- Late stages of lung differentiation
Time
Topic
8:00-9:00
Breakfast (Provided)
9:00-9:30
Lecture 5: Optimization of directed differentiation towards
mature airway epithelia cells by Dr. Amy Wong
9:30-9:45
Break
9:45-10:15
Practical 10: Observe and discussion of differentiation stage 3a;
differentiation into lung progenitor cells
10:15-10:45
Practical 11: Observe and discussion of differentiation stage 3b;
differentiation into immature lung cells
10:45-11:00
Break
11:00-11:30
Practical 12: Observe and maintenance of cultures prepared on
Day 1 and Day 2
11:30-12:00
Practical 13: Media change for transwell cultures
12:00-1:00
Lunch (Provided)
1:00-2:00
Lecture 6: Media systems to support epithelia cell generation by
Lonza
2:00-2:15
Break
2:15-2:45
Practical 12: Observe and discussion of differentiation stage 4;
expansion of immature lung cells
2:45-3:15
Practical 13: Observe and discussion of differentiation stage 5;
maturation and polarization of epithelium
3:15-3:30
Coffee Break (Provided)
3:30-4:15
Lecture 7: Utility of nasal cell cultures as a model system by Dr.
Theo Moraes
4:15-5:00
Lecture 8: Functional assessment of the response of CFTRtargeting drugs in nasal epithelial cells from CF patients by Dr.
Tanja Gonska
(at SickKids)
Day 4- Characterization of CFTR function of mature lung epithelium
Time
Topic
8:00-9:00
Breakfast (Provided)
9:00-10:00
Lecture 9: Evolving models for in vitro testing of patient specific
responses to emerging drugs targeting Cystic Fibrosis by Dr.
Christine Bear
10:00-10:15
Break
10:15-12:00
Practical 14: Functional characterization of CFTR function
12:00-12:30
Course wrap-up
**Final schedule will be confirmed close to the workshop date**
Sponsors include: