PROPER SET UP FOR HOME-CAGE EUTHANASIA
This port should be left
open to allow displaced
oxygen to exit the cage.
This tube carries the CO2
from the flow meter and
should fit tightly into the
port.
Watch the ball to set
the appropriate flow
rate for the cage
you’re euthanizing.
This example shows
the rate for a small
mouse cage.
1. Remove the lid and wirebar feed hopper.
2. Place the euthanasia lid on
the home-cage.
3. Turn on the CO2 tank.
4. Turn on the flow meter to
the proper rate for the cage
being euthanized (stated in
euthanasia SOP).
6. Once all animals have
ceased breathing, allow 2
minutes to elapse to ensure
death.
7. Turn off the CO2 tank.
8. Turn off the flow meter.
9. Perform secondary method
and place in freezer.
Turn this knob to set
the flow rate and to
turn it on and off.
Flow rate for Transgenics: Small Mouse Cage = 1.89 L/min., Large Mouse Cage = 5.16 L/min., Rat Cage = 7.02 L/min.