Fruto do pawpaw – Carica papaya como antioxidante pancreático 2 trabalhos
Inhibition of key enzymes linked to type 2 diabetes and sodium
nitroprusside-induced lipid peroxidation in rat pancreas by
water-extractable phytochemicals from unripe pawpaw fruit
(Carica papaya).
Oboh G, Olabiyi AA, Akinyemi AJ, Ademiluyi AO.
J Basic Clin Physiol Pharmacol. 2013 May 30:1-14. doi: 10.1515/jbcpp-2013-0002. [Epub ahead
of print]
Abstract
Abstract Background: Various parts of unripe pawpaw (Carica papaya Linn) fruit have been
reportedly used for the management or treatment of diabetes mellitus in folklore medicine.
Therefore, the present study sought to investigate the inhibitory effects of the aqueous extract of
different parts of unripe pawpaw fruit on key enzymes linked to type 2 diabetes (α-amylase and
α-glucosidase) and sodium nitroprusside (SNP)-induced lipid peroxidation in rat pancreas in
vitro. Methods: The aqueous extracts of the unripe pawpaw (C. papaya) fruit parts were
prepared (1:20 w/v) and the ability of the extracts to inhibit α-amylase, α-glucosidase and SNPinduced lipid peroxidation in rat pancreas in vitro was investigated. Results: The results
revealed that all the extracts inhibited α-amylase (IC50=0.87-1.11 mg/mL), α-glucosidase
(IC50=1.76-2.64 mg/mL) and SNP-induced lipid peroxidation (IC50=1.99-2.42 mg/mL) in a
dose-dependent manner. However, combination of the flesh, seed and peel in equal amounts
had the highest inhibitory effect on α-amylase and α-glucosidase activities. Conclusions: Strong
inhibitory activities of the unripe pawpaw fruit against key enzymes linked to type 2 diabetes and
SNP-induced lipid peroxidation in rat pancreas could be part of the mechanism by which unripe
pawpaw is used in the management/prevention of diabetes mellitus in folk medicine. However,
combining the unripe pawpaw fruit parts in equal amounts exhibited synergistic properties on αamylase and α-glucosidase inhibitory activities.
PMID:23740684
Inhibitory effect of aqueous extract of different parts of unripe
pawpaw (Carica papaya) fruit on Fe²⁺-induced oxidative stress
in rat pancreas in vitro.
Oboh G, Olabiyi AA, Akinyemi AJ.
Pharm Biol. 2013 Sep;51(9):1165-74. doi: 10.3109/13880209.2013.782321. Epub 2013 Jun 12.
Source
Functional Foods and Nutraceuticals Unit, Department of Biochemistry, Federal University of
Technology, Akure, Nigeria. [email protected]
Abstract
CONTEXT:
Carica papaya L. (Caricaceae) is widespread throughout tropical Africa; it is cultivated for its
fruits and it is eaten in various ways.
OBJECTIVE:
This study sought to investigate the inhibitory effect of the aqueous extract of different parts of
unripe pawpaw fruit on Fe²⁺-induced lipid peroxidation in rat's pancreas in vitro.
MATERIALS AND METHODS:
The aqueous extract of the unripe pawpaw fruit parts; peel (PG), seed (SG), flesh (FG), flesh
with peel (FPG) and a combination of equal amount of all parts (CG) were prepared, the total
phenolic content and the antioxidant activities of the extracts were then evaluated using various
spectrophotometric methods.
RESULT:
PG had the highest total phenol content (1.24 mg GAE/g), flavonoid content (0.63 mg QUE/g),
reducing power (7.07 mg AAE/g) and Fe²⁺ chelating ability while the SG had the highest 1,1diphenyl-2-picrylhydrazyl radical scavenging ability. Furthermore, all the extracts caused a
significant decrease (p < 0.05) in the malondialdehyde contents in the pancreas with SG
(IC₅₀ = 4.25 mg/mL) having the highest inhibitory effect on Fe²⁺-induced lipid peroxidation.
DISCUSSION AND CONCLUSION:
This protective effect of the extracts on Fe²⁺-induced lipid peroxidation in rat pancreas could be
attributed to their phenolic compounds and, the possible mechanism may be through their
antioxidant activities. However, the effect of combination of different parts of unripe pawpaw fruit
in equal amount (w/w) on the inhibition of Fe²⁺-induced lipid peroxidation in rat pancreas
exhibited additive properties.
PMID:23758107