From: Fundus Autofluorescence Findings in a Mouse Model of Retinal Detachment
Invest. Ophthalmol. Vis. Sci.. 2012;53(9):5190-5197. doi:10.1167/iovs.12-9672
Figure Legend:
Fluorescence emission spectra and corresponding confocal microscopic images demonstrating recording areas on and
off autofluorescent spots. (A) Fluorescence spectra obtained using flat-mounted Abca4 −/− mouse retina in the presence
of an RD. Data were recorded on spot (B; field size 512 × 512 pixels, 0.004 um per pixel) and off spot (C; field size 512
× 512 pixels, 0.010 um per pixel) using a confocal laser scanning fluorescence microscope 6 nm increments, and laser
excitation at 488 and 561 nm as indicated. Emission data were adjusted for laser power. Peak emission wavelengths are
indicated adjacent to each trace. Note red-shift with increasing excitation wavelength. The difference between the two
excitation
wavelengths
is noticeable The
withAssociation
the on-spotforrecording.
Date
of download:
7/31/2017
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