HPLC Application
ID No.: 16972
Glutathione oxidized and reduced on Synergi Hydro-RP
Column:
Dimensions:
Order No:
Elution Type:
Eluent A:
Eluent B:
Gradient
Profile:
Flow Rate:
Col. Temp.:
Detection:
Synergi™ 4 µm Hydro-RP 80 Å, LC Column 150 x 4.6 mm, Ea
150 x 4.6 mm ID
00F-4375-E0
Isocratic
20mM Potassioum phosphate pH 2.7
Acetonitrile
Step No.
Time (min)
Pct A
Pct B
0
99
1
1
1 mL/min
ambient
UV-Vis Abs.-Variable Wave.(UV) @ 210 nm (ambient)
ANALYTES:
1
Glutathione reduced form
2
Glutathione oxidized form (dimer)
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