Date of Document: July 31, 2017
CURRICULUM VITAE
Balaraman Kalyanaraman PhD
Chair, Professor
Department of Biophysics
OFFICE ADDRESS:
Medical Education Building
8701 Watertown Plank Rd
Milwaukee, WI 53226
CITIZENSHIP: USA
EDUCATION:
07/1970 B.S., Chemistry, Loyola College
07/1972 M.S., Chemistry, Indian Institute of Technology
05/1978 Ph.D., Chemistry, University of Alabama
POSTGRADUATE TRAINING AND FELLOWSHIP APPOINTMENTS:
1978 - 1981 Visiting Fellow, Laboratory of Environmental Biophysics, National Institute of Environmental
Health Sciences, Research Triangle Park, North Carolina
1981 - 1984 Research Associate (Postdoctoral), National Biomedical ESR Center, Medical College of
Wisconsin, Milwaukee, WI
MILITARY SERVICE:
n/a
FACULTY APPOINTMENTS:
1984 - 1989 Assistant Professor of Radiology, Medical College of Wisconsin, Milwaukee, WI
1989 - 1994 Associate Professor of Radiology, Medical College of Wisconsin, Milwaukee, WI
1994 - Present Professor of Biophysics, Medical College of Wisconsin, Milwaukee, WI
1997 - Present Professor of Pediatrics, Medical College of Wisconsin, Milwaukee, WI
2009 - Present Harry R. & Angeline E. Quadracci Professor in Parkinson's Research, Medical College of
Wisconsin, Milwaukee, WI
ADMINISTRATIVE APPOINTMENTS:
1998 - 2000 Interim Chairman and Director, Biophysics Research Institute, Medical College of Wisconsin,
Milwaukee, WI
2000 - 2003 Director, Biophysics Research Institute, Medical College of Wisconsin, Milwaukee, WI
2000 - Present Director, Free Radical Research Center, Medical College of Wisconsin, Milwaukee, WI
2000 - 2006 Associate Director, Cardiovascular Research Center, Medical College of Wisconsin, Milwaukee,
WI
2003 - Present Chairman, Department of Biophysics, Medical College of Wisconsin, Milwaukee, WI
EDUCATIONAL ADMINISTRATIVE APPOINTMENTS:
n/a
HOSPITAL AND CLINICAL ADMINISTRATIVE APPOINTMENTS:
n/a
RESEARCH ADMINISTRATIVE APPOINTMENTS:
1972 - 1978 OTHER RESEARCH EMPLOYMENT: Technical Development Chemist, CIBA-GEIGY of
India, Ltd., Bombay, India
HOSPITAL STAFF PRIVILEGES:
n/a
SPECIALTY BOARDS AND CERTIFICATION:
Board Certified
DABT (Toxicology), Certified Diplomate of the
American Board of Toxicology
Issue Date
Expiration
11/1984
None
AWARDS AND HONORS:
2001 Recipient, International EPR Society Silver Medal for Biology and Medicine
2005 Recipient, Lifetime Achievement Award, Society for Free Radical Biology and Medicine
2010 Ad Honorem Professor of Medicine (for distinguished merit and support of the Department of
Biochemistry), School of Medicine, University of the Republic, Montevideo, Uruguay
MEMBERSHIPS IN HONORARY AND PROFESSIONAL SOCIETIES:
American Association for the Advancement of Science (AAAS)
American Chemical Society (ACS)
Society for Free Radical Biology and Medicine (SFRBM)
Federation of American Societies for Experimental Biology (FASEB)
International EPR (ESR) Society
Biophysical Society
EDITORSHIPS/EDITORIAL BOARDS/JOURNAL REVIEWS:
Editorship
1992 - 2014 Free Radicals in Biology and Medicine
2006 - 2007 Free Radical Research
Editorial Board
2001 - 2008 Biochemical Journal
Journal Review
Free Radical Research
Free Radicals in Biology and Medicine
Journal of Molecular Biology
Journal of Neurochemistry
Journal of Neuroscience
Medical Physics
Nitric Oxide: Biology and Chemistry
Oncogene
Photochemistry and Photobiology
PLOS ONE
Proceedings of the National Academy of Sciences
Toxicology and Applied Pharmacology
American Journal of Physiology – Heart and Circulatory Physiology
Cancer Prevention Research
Biochemical Journal
Biochimica et Biophysica Acta – Bioenergetics
British Journal of Cancer
Cancer Biology and Therapy
Cancer Research
Chemical Reviews
Circulation
Circulation Research
Journal of the American Chemical Society
Journal of Biological Chemistry
Journal of Neural Transmission
Journal of Organic Chemistry
Journal of Pharmacology and Experimental Therapeutics
Medical Principles and Practice
Nature Protocols
Trends in Biochemical Sciences
Biochimica et Biophysica Acta – General Subjects
Arteriosclerosis, Thrombosis, and Vascular Biology
Biochemistry
Chemical Research in Toxicology
Chemical Physics Letters
Archives of Biochemistry and Biophysics
Annals of Biomedical Engineering
LOCAL/REGIONAL APPOINTED LEADERSHIP AND COMMITTEE POSITIONS:
2009 - 2011 Chair, Biomedical Resource Center (BRC) Advisory Committee, Medical College of Wisconsin
2010 - Present Co-Director, Cancer Cell Biology Research Program, Medical College of Wisconsin
2010 - Present Member, Leadership Committee, Cancer Center, Medical College of Wisconsin
NATIONAL ELECTED/APPOINTED LEADERSHIP AND COMMITTEE POSITIONS:
1987 Reviewer, Cancer Center Study Section, Medical College of Wisconsin
1987 - 1988 Reviewer, Oxy-radicals Special Study Section, National Institutes of Health
1989 Reviewer, Electron Spin Resonance Special Study Section, National Institutes of Health
1990 Reviewer, Instrumentation Special Study Section, National Institutes of Health
1990 Member, Site visit team, National Institutes of Health - Spin Trapping Center at Oklahoma
1991 Member, Site visit team, AFRI
1992 Member, Five-year review committee for Radiation Biology Section, National Cancer Institute, NIH
1993 Reviewer, Special Study Section, National Institute of Arthritis and Musculoskeletal and Skin Diseases,
NIH
1993 Reviewer, Study Section, Oklahoma Center for the Advancement of Science and Technology
1994 Special Reviewer, Biophysical Chemistry Study Section, National Institutes of Health
1994 Reviewer, Physical Biochemistry Section, Oklahoma Center for the Advancement of Science and
Technology
1994 Member, Site visit team, Program Project at Dartmouth College of Medicine, NIH
1994 Member, Site visit team, Program Project at Iowa College of Medicine, NIH
1994 Member, Intramural site visit team, Radiation Biology Section, National Cancer Institute, NIH
1995 Reviewer, Special Study Section, Program Project, National Cancer Institute, NIH
1995 Member, Intramural site visit team, National Institute of Mental Health, NIH
1995 Reviewer, Radiation Research Study Section, National Institutes of Health
1996 Member, Ad Hoc Chemical Pathology Study Section, National Institutes of Health
1996 Member, Site visit team, EPR Center at Dartmouth, NIH
1997 Chairman, Pharmacology Study Section, Oklahoma Center for the Advancement of Science and
Technology
1997 Intramural Reviewer, Radiation Biology
1998 Member, Site visit team, EPR Center in Chicago, NIH
1998 Vice-Chairperson, Gordon Research Conference on Oxygen Radicals in Biology
1998 Member, Site visit team, EPR Center in New York, NIH
1999 Chairman, Biochemistry Study Section, Oklahoma Center for the Advancement of Science and
Technology
1999 Member, Geriatrics, Rehabilitation, and Medicine Teleconference Study Section, National Institutes of
Health
2000 Member, Biochemistry Study Section, Oklahoma Center for the Advancement of Science & Technology,
Oklahoma City
2000 Member, Site visit team, National Cancer Institute, NIH, University of Iowa
2000 Chairperson, Gordon Research Conference on Oxygen Radicals in Biology
2001 Member, Site visit team, Program Project Grant on Tissue Oxygen Determination, NIH, Dartmouth, NH
2001 Co-Chairperson, 5th Workshop on EPR Applications in Biology and Medicine, Krakow, Poland
2002 Intramural Reviewer, Radiation Biology
2003 Member, Ad Hoc Experimental Cardiovascular Sciences Study Section, National Institutes of Health
2004 Member, Ad Hoc ECS Study Section, National Institutes of Health
2005 - 2008 Vice President - Americas, International EPR (ESR) Society
2005 Member, National Advisory Board, International Conference on Electron Paramagnetic Resonance
Spectroscopy and Imaging of Biological Systems (EPR 2005), Columbus, OH
2007 - 2011 Member, Xenobiotic and Nutrient Disposition and Action Study Section, Center for Scientific
Review, NIH
2007 Co-Chair, EPR2007: A Joint Conference of the 12th In Vivo EPR Spectroscopy & Imaging and the 9th
International EPR Spin Trapping/Spin Labeling, April 29-May 3, 2007, Chicago
2007 Co-Chair, Reactive Oxygen/Nitrogen Species Symposium, American Society of Nephrology, October
31-November 5, 2007, San Francisco
2009 - 2013 Chair, 2012 Biology/Medicine Silver Award Committee, International EPR Society
2009 Member, Intramural site visit team, National Cancer Institute, NIH
2009 Co-Chair, Nominations/Leadership Development Committee, Society for Free Radical Biology and
Medicine
2009 Ad Hoc Reviewer, Molecular Dynamics Section and Brain Physiology and Metabolism Section, National
Institute on Aging, NIH
2009 Co-Chair, Free Radical School Committee, Society for Free Radical Biology and Medicine
2009 HLBP Workgroup, PPG Review, National Heart Lung and Blood Institute, NIH
2009 - 2013 Chair, IES Biology/Medicine Silver Award Committee, International EPR (ESR) Society
2010 Co-Chair, VIIIth International Workshop on EPR (ESR) in Biology and Medicine, October 4-7, 2010,
Krakow, Poland
2010 Co-Chair, Mini-Symposium on Parkinson’s Disease, Medical College of Wisconsin
2010 Co-Chair, EPR2010: A Joint Conference of the 14th In Vivo EPR Spectroscopy & Imaging and the 11th
International EPR Spin Trapping/Spin Labeling, May 2-6, 2010, San Juan, Puerto Rico
2013 Co-Chair, IXth International Workshop on EPR (ESR) in Biology and Medicine, October 7-10, Krakow,
Poland
2014 Co-Chair, First Adriatic Symposium on Biophysical Applications in Biomedical Studies, August 24-28,
2014, Split, Croatia
2014 Member, Gold Medal Award Committee, International EPR Society
2015 Member, Review Committee, Research Center of Redox Processes in Biomedicine, CEPID Redoxoma,
São Paulo Research Foundation, Universidade de São Paulo, Brazil
2016 Chair, Review Committee, National Cancer Institute Special Emphasis Panel to review SBIR proposals
on “Highly Innovative Tools for Quantifying Redox Effector Dynamics in Cancer” (ZCA1 TCRB-D
(C1))
2016 Ad hoc Reviewer, Chemo/Dietary Prevention Study Section (CDP), June 23-24, 2016, Seattle, WA
RESEARCH GRANTS/AWARDS/CONTRACTS/PROJECTS:
Active
Peer Review
Title:
Source:
Role:
PI:
Dates:
Title:
Source:
"Targeting Pancreatic Cancer Energy Metabolism, Tumor Growth, and
Metastasis"
NIH/NCI (U01 CA178960-01A1)
Multiple PI
Dwinell/Kalyanaraman
07/01/2014 - 05/31/2019
"CYP2E1 Mediated Mitochondrial Injury and Cell Damage in Alcohol Liver
Disease"
NIH/NIAAA (1R01AA022986-01A1)
Role:
PI:
Dates:
Pending
Peer Review
Title:
Source:
Role:
Dates:
Multiple PI
Avadhani/Kalyanaraman
02/05/2015 - 12/31/2019
"Basic and Translational Biology of Pancreas Cancer" (PPG)
NIH
Project Leader, Project 2, "Targeting Inhibitors of Nox &
Mitochondria/Glycolytic Metabolism in PDAC therapy"
04/01/2016 - 03/31/2021
Source:
Role:
Dates:
"Targeting Inhibitors of Nox & Mitochondrial/Glycolytic Metabolism in
PDAC Therapy"
NIH
Multiple PI
04/01/2016 - 03/31/2021
Title:
Source:
Role:
Dates:
"Biased GPCR Signaling in Pancreas Cancer Progression and Metastasis"
NIH
Co-investigator
04/01/2016 - 03/21/2021
Title:
Source:
Role:
PI:
Dates:
"Macrophage Redox State in Sterilizing and Injurious Inflammation"
NIH/NHLBI
Subcontract PI
Bonini
06/01/2016 - 05/31/2021
Title:
Source:
Role:
Dates:
"Chemoprevention of Lung Cancer with Mitochondria-targeted Honokiol"
NIH/NCI
Multiple PI
07/01/2016 - 06/30/2021
Title:
Source:
Role:
Dates:
"Credentialing preclinical models for cardio-oncology studies"
NIH
Multiple PI (Flister/Kalyanaraman)
07/01/2016 - 06/30/2019
Title:
Source:
Role:
PI:
Dates:
A Novel Paradigm for Comprehensive Redox Profiling"
NIH
Multiple PI
Benjamin/Darley-Usmar/Gutterman/Kalyanaraman/Link
08/01/2016 - 07/31/2021
Title:
Source:
Role:
"Mitochondria-targeted Metformin Analogs in Radiosensitization"
NIH
Multiple PI
Title:
Prior
Peer Review
Title:
Source:
Role:
PI:
Dates:
“Radical Reactions of Catechols, Phenols and Quinones”
NIH (R01 GM209035)
Principal Investigator
Kalyanaraman
04/01/1985 - 07/31/1988
Title:
Source:
Role:
PI:
Dates:
“Phototoxicity and Protection in Pigmented Systems”
NIH (AM026950)
Principal Investigator
Kalyanaraman
08/01/1985 - 07/31/1986
Title:
Source:
Role:
PI:
Dates:
“Free Radical Mechanism of Lignin Biodegradation”
Forest Products Laboratory
Principal Investigator
Kalyanaraman
1985 - 1986
Title:
Source:
Role:
PI:
Dates:
“Free Radical Mechanism of Degradation of Lignin Model Compounds”
Forest Products Laboratory
Principal Investigator
Kalyanaraman
09/01/1987 - 04/30/1989
Title:
Source:
Role:
Dates:
“Development of Hypoxic Metabolism Probe Suitable for Use in MRI”
Cancer Center, Medical College of Wisconsin
Principal Investigator
07/01/1988 - 06/30/1989
Title:
Source:
Role:
PI:
Dates:
“Mechanism of Merocyanine-Mediated Phototherapy”
NIH/NCI (CA049089)
Principal Investigator, Project 1 (PI: Albert Girotti)
Kalyanaraman
01/01/1989 - 12/31/1991
Title:
Source:
Role:
PI:
Dates:
“Age Reactive Oxygen Metabolites and Myocardial Injury”
NIH
Co-Principal Investigator
Kalyanaraman
08/01/1989 - 08/01/1994
Title:
Source:
Role:
PI:
Dates:
“Radical Generation during Cardiac Surgery”
NIH/NHLBI (R01 HL045048)
Principal Investigator
Kalyanaraman
07/01/1990 - 11/30/1997
Title:
Source:
Role:
Dates:
“Free Radical Mechanism of Lignin Biodegradation”
Forest Products Laboratory
Principal Investigator
1990 - 1991
Title:
Source:
Role:
Dates:
“Low Density Lipoprotein in Free Radical Oxidative Damage”
NIH/NHLBI (R01 HL047250)
Principal Investigator
08/01/1991 - 08/01/2000
Title:
Source:
Role:
PI:
Dates:
“Mechanism of Merocyanine-Mediated Phototherapy”
NIH/NCI
Principal Investigator (Project 1)
Kalyanaraman
12/01/1992 - 11/30/1995
Title:
Source:
Role:
PI:
Dates:
“Radicals in Cardiac Surgery”
Medical College of Wisconsin
Principal Investigator
Kalyanaraman
07/01/1993 - 06/30/1994
Title:
Source:
Role:
Dates:
“Radicals in Cardiac Surgery”
NIH
Principal Investigator
01/01/1994 - 12/31/1997
Title:
Source:
Role:
Dates:
“Iron, ∙NO, and Lipid Peroxides in Photodynamic Therapy”
NIH
Co-Investigator
01/10/1996 - 01/10/1999
Title:
Source:
Role:
PI:
Dates:
“Nitric Oxide and Tolerance of Hypoxic Heart to Ischemia”
NIH
Co-Principal Investigator
Kalyanaraman
04/01/1997 - 03/31/2001
Source:
Role:
PI:
Dates:
“Radical Generation from FALS-Specific SOD Mutants - ESR Spin-Trapping
and Multifrequency Studies”
ALS Association
Principal Investigator
Kalyanaraman
02/01/1998 - 01/31/1999
Title:
Source:
Role:
PI:
Dates:
“eNOS and Radical Mechanism of Antitumor Anthracyclines”
NIH/NCI (CA077822)
Principal Investigator
Kalyanaraman
01/01/1999 - 08/31/2008
Title:
Title:
Source:
Role:
PI:
Dates:
“Peroxynitrite Mediated Mechanisms in Cerebral Ischemia”
NIH (NS38133)
Co-Investigator
William Chilian
01/1999 - 12/31/2001
Title:
Source:
Role:
PI:
Dates:
“Nitric Oxide-Mediated Oxidation/Nitration in Membranes”
NIH/NHLBI (R01 HL063119)
Principal Investigator
Kalyanaraman
07/01/1999 - 03/31/2012
Source:
Role:
PI:
Dates:
“Bicarbonate-induced Oxidative Formation from FALS - specific SOD
Mutants”
ALS Association
Principal Investigator
Kalyanaraman
02/01/2000 - 01/31/2003
Title:
Source:
Role:
PI:
Dates:
“Role of Neuronal NOS and Superoxide in Neurodegeneration”
NIH/NINDS (NS039958)
Principal Investigator
Kalyanaraman
04/10/2000 - 03/31/2008
Title:
Source:
Role:
PI:
Dates:
“Bicarbonate Enhances Peroxidation of SOD/ALS Mutants”
NIH/NINDS (R01 NS040494)
Principal Investigator
Kalyanaraman
07/01/2000 - 01/31/2010
Title:
Source:
Role:
PI:
Dates:
“Pathogenesis of Herpetic Keratitis”
NIH (EY012863)
Co-Investigator
William O’Brien
08/01/2000 - 07/31/2003
Title:
Source:
Role:
PI:
Dates:
“Nitric Oxide Synthase: Enzymology and Metabolic Role”
NIH (DK48423)
Co-Investigator
Owen Griffith
08/01/2000 - 06/30/2005
Title:
Source:
Role:
PI:
Dates:
“Vascular Signaling by Free Radicals” – Core B: Free Radical Core Facility
NIH/NHLBI (HL68769)
Director, Core B
David Harder
04/01/2002 - 03/31/2007
Title:
Title:
Source:
Role:
PI:
Dates:
Title:
Source:
Role:
PI:
Dates:
Title:
Source:
Role:
PI:
Dates:
“Peroxide, NO and Iron Signaling in Endothelial Damage”
NIH/NHLBI (HL073056)
Principal Investigator
Kalyanaraman
03/17/2003 - 02/28/2009
“Mito-Q Attenuates DOX-induced Cardiotoxicity and Potentiates Antitumor
Effects: MR Imaging and Echocardiography Studies”
Wisconsin Breast Cancer Showhouse
Principal Investigator
Kalyanaraman
02/01/2007 - 07/31/2009
“Development of Mitochondrially Targeted Antioxidants for Diabetic
Therapy”
NIH/NIDDK (R01 DK075865)
Subcontract PI
Darley-Usmar
04/01/2007 - 03/31/2011
Source:
Role:
PI:
Dates:
“Ang II, RAGE and Oxidative Stress in Type II Diabetic Coronary Artery
Remodeling”
NIH/NHLBI (R01 HL056046)
Subcontrac
Lucchesi
07/01/2007 - 06/30/2013
Title:
Source:
Role:
PI:
Dates:
“Superoxide Production and Functions in Corneal Cells”
NIH/NEI (R01 EY017079)
Investigator
O’Brien
08/01/2007 - 08/31/2012
Title:
Source:
Role:
PI:
Dates:
“Redox-control of MMP-1 and Senescence”
NIH (R01 AG031067)
Subcontract PI
Melendez
04/01/2008 - 03/31/2013
Title:
Source:
Role:
PI:
Dates:
Role of iNOS, Nitric Oxide & Arginase in Statin-mediated
NIH / NCI
PI
Kalyanaraman
07/01/2008 - 05/31/2013
Title:
Source:
Role:
PI:
Dates:
Neuroprotection by Mitochondria-targeted Antioxidants
NIH / NINDS
multiple PI
Kalyanaraman / Kanthasamy
02/01/2009 - 01/31/2014
Title:
Title:
Source:
Role:
PI:
Dates:
Mitochondria-targeted Agents in Breast Cancer
NIH / NCI
PI
Kalyanaraman B
06/14/2010 - 04/30/2015
INVITED LECTURES/WORKSHOPS/PRESENTATIONS:
Local
Pharmacology and Toxicology, Medical College of Wisconsin, 1985
Neurology, Medical College of Wisconsin, 2004
Biophysics Graduate Seminar Series, Medical College of Wisconsin, 2004
Biochemistry, Medical College of Wisconsin, 2006
Biophysics Graduate Seminar Series, Medical College of Wisconsin, 2006
Thirteenth Annual Alzheimer's Disease Symposium, Medical College of Wisconsin, 2008
MCW Parkinson's Team, Froedtert Memorial Lutheran Hospital, 2008
Blood Research Institute, 2010
Mini-Symposium on Basic Mechanisms and Treatment of Parkinson's Disease, Milwaukee, WI, 2010
Medical College of Wisconsin, 2010
Biophysics Graduate Seminar Series, Medical College of Wisconsin, 2011
Brain Tumor Board Meeting, Medical College of Wisconsin, 2011
Biophysics Graduate Seminar Series, Medical College of Wisconsin, 2012
Cancer Cell Biology Research Forum, Medical College of Wisconsin, 2013
Focus on Technology Lecture Series, Blood Research Institute, Milwaukee, WI, 2014
Biophysics Graduate Seminar Series, Medical College of Wisconsin, 2016
Regional
S.C. Johnson and Son, Inc., Racine, WI, 1985
Abbott Laboratories, Abbott Park, IL, 2011
National
Department of Biochemistry, University of Nevada at Reno, Reno, Nevada, 1985
Symposium on Free Radical Metabolites, American Society of Pharmacology and Experimental Therapeutics,
Boston, MA, 1985
Southeastern Magnetic Resonance Conference, Research Triangle Park, NC, 1987
Department of Dermatology, Case Western University, OH, 1989
Merrill Dow Chemicals, Cincinnati, OH, 1989
National Institute of Environmental Health Science, Research Triangle Park, NC, 1989
Department of Pharmacology, Wayne State University, Detroit, MI, 1989
National Institute of Environmental Health Science, Research Triangle Park, NC, 1990
"Oxidative Damage and Repair", Society for Free Radical Research, Pasadena, CA, 1990
Southeastern Magnetic Resonance Conference, Tuscaloosa, AL, 1990
National Cancer Institute, Bethesda, MD, 1991
National Institute of Environmental Health Science, Research Triangle Park, NC, 1991
Society of Toxicology, Seattle, WA, 1992
Oxygen Society Meeting, Charleston, SC, 1993
International Spin Trapping Symposium, OK, 1993
Gordon Research Conference on Oxy-Radicals, Ventura, CA, 1994
Radiation Biology, NCI, NIH, 1994
Photobiology Symposium, Scottsdale, AZ, 1994
International Cation Radicals Symposium, Raleigh, NC, 1994
International Pigment Cell Conference, Philadelphia, PA, 1994
XVII International Conference on Magnetic Resonance in Biological Systems, Keystone, CO, 1996
Nitric Oxide Symposium, Rocky Mountain Conference, Denver, CO, 1996
NIEHS, Research Triangle Park, NC, 1997
Biochemistry Branch, NHLBI, 1997
Oxygen Club of California, Santa Barbara, CA, 1997
Rocky Mountain ESR Conference, Denver, CO, 1997
Biochemistry Department, Ohio State University, OH, 1997
Photochemistry Photobiology Meeting, Nitric Oxide Symposium, St. Louis, MO, 1997
Department of Pathology, University of Vermont, 1997
Cancer Center, Vanderbilt University, 1997
International Oxygen Society Meeting, Washington DC, 1998
Department of Anesthesiology, University of Alabama, Birmingham, AL, 1998
Oxygen Club of Washington DC, 1998
Oxygen Club, NIEHS, 1998
Vascular Biology Seminar Series, Cardiology Department, Emory University, Atlanta, GA, 1999
Oxygen Club of California-Santa Barbara, 1999
Environmental Toxicology Program, Vanderbilt University, 2000
American Society of Photobiology, Chicago, IL, 2001
EPR Workshop, Dartmouth, NH, 2001
EPR Symposium, Denver, CO, 2001
Oxygen Club of California, Santa Barbara, CA, 2001
Southeastern Magnetic Resonance Conference, Research Triangle Park, NC, 2002
NIEHS, Research Triangle Park, NC, 2002
LSU Medical Center, Shreveport, LA, 2002
Reactive Oxygen and Nitrogen Intermediates, Morgantown, WV, 2002
EPR Symposium, Denver, CO, 2002
FASEB Vascular Biology Symposium, New Orleans, LA, 2002
Massachusetts General Hospital Cardiovascular Research Center, Boston, MA, 2003
Indiana University Medical School, Indianapolis, IN, 2003
American Chemical Society, Toxicology Division, New York, 2003
Lawrence Piette Memorial Lecture, Denver, CO, 2003
University of Alabama, Birmingham, AL, 2003
American Chemical Society, New York, 2003
Gordon Research Conference on Oxygen Radicals, Ventura, CA, 2004
American Heart Association Scientific Sessions, New Orleans, LA, 2004
Third Annual Symposium on Oxidative Stress and Nutrition, University of Kentucky, Lexington, KY, 2004
Distinguished Scientist Seminar Series, University of South Alabama, Mobile, AL, 2005
Society for Free Radical Biology and Medicine Conference, Austin, TX, 2005
Anatomy and Cell Biology, University of North Dakota, Grand Forks, North Dakota, 2005
NIEHS, Research Triangle Park, NC, 2005
2005 Electron Paramagnetic Resonance Conference, Columbus, Ohio, 2005
Biomedical Sciences and Neuroscience Graduate Program, Iowa State University, Ames, IA, 2005
Laboratory of Pharmacology and Chemistry, NIEHS, Research Triangle Park, NC, 2006
Department of Chemistry, North Carolina State University, Raleigh, NC, 2006
Northwestern University, Chicago, IL, 2007
American Society of Nephrology - Renal Week 2007, 2007
NIEHS, Research Triangle Park, NC, 2007
Linus Pauling Institute, Oregon State University, 2007
UAB Center for Free Radical Biology & Department of Pathology, University of Alabama, Birmingham, AL,
2008
Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, TN, 2008
Society of Free Radical Biology in Medicine, Indianapolis, IN, 2008
Gordon Research Conference, Ventura, CA, 2008
Kispert Symposium, University of Alabama, Tuscaloosa, AL, 2009
Center for Systems Biology, University of Chicago, 2009
Midwest Breast Cancer Research Symposium, Holden Comprehensive Cancer Center, University of Iowa,
Iowa City, IA, 2009
Mari Lowe Center for Comparative Oncology, University of Pennsylvania, Philadephia, PA, 2010
Experimental Biology 2011, Washington DC, 2011
Department of Pharmacology, University of Illinois at Chicago, 2011
NIEHS, Research Triangle Park, NC, 2012
Biochemistry and Redox Biology Center, University of Nebraska-Lincoln, 2012
Gordon Research Conference on Oxygen Radicals, Ventura, California, 2012
Molecular Biosciences Graduate Student Forum, University of Nevada, Reno, NV, 2013
Clinical Pharmacology Grand Rounds, Vanderbilt University, 2014
Department of Chemistry and Biochemistry, University of California, Santa Cruz, CA, 2014
Graduate Education Seminar Series, Burnett School of Biomedical Sciences, University of Central Florida
College of Medicine, Orlando, FL, 2014
Biochemistry Seminar, Northwestern University, Chicago, IL, 2015
Redox Symposium, Society for Redox Biology and Medicine, University of Illinois at Chicago, Chicago, IL,
2016
International
Free University, Department on Oncology, Amsterdam, 1985
Universite de Liege, Institut de Physique, Belgium, 1985
The Japanese Free Radical Society Meeting, Kyoto, Japan, 1988
5th International Congress on Oxygen Radicals, Kyoto, Japan, 1991
3rd International Symposium on Spin Trapping, Kyoto, Japan, 1991
Burroughs-Wellcome, Kent, England, 1991
Melanoma Meeting, Manchester, England, 1991
Biochemical Society, London, 1992
International Conference on Free Radicals, Torino, Italy, 1992
International Symposium on Oxy-Radicals, Vienna, Austria, 1993
University of Grenoble, France, 1993
Humacao University College, University of Puerto Rico, Puerto Rico, 1994
Oxy-Radical Satellite Meeting, Queenstown, New Zealand, 1994
Biochemical Society Meeting, Brighton, UK, 1994
Free Radicals in Nutrition, Biochemical Society, Brighton, UK, 1994
London Free Radical Forum, London, 1996
First International Meeting on Peroxynitrite in Chemistry and Biology, Ascona, Switzerland, 1997
International Meeting on Free Radicals, Sao Pãolo, Brazil, 1998
International Conference on Nitric Oxide, Graz, Austria, 1998
International Nitric Oxide Meeting, Singapore, 1999
International Meeting on ESR Spin Trapping, Marseille, France, 2000
ISFRR Meeting, Paris, 2002
Kyushu University, Fukuoka, Japan, 2003
Polish Biochemical Society, Gdansk, Poland, 2003
Society of Electron Spin Science and Technology, Hiroshima University, Hiroshima, 2003
International Conference on Natural Products, Free Radicals and Radioprotectors in Health, Chidambaram,
India, 2004
XII Meeting of the Society for Free Radical Research International, Buenos Aires, Argentina, 2004
Chemistry and Therapeutic Applications of Nitric Oxide, Third International Conference on Biology, Nara,
Japan, 2004
Seventh International ISSX (International Society for the Study of Xenobiotics) Meeting, Vancouver, Canada,
2004
Sixth Workshop on Applications of EPR in Biology and Medicine, Krakow, Poland, 2004
Humacao, Puerto Rico, 2004
Symposium on Free Radicals and Antioxidants in Food Sciences, Society for Free Radical Research-India,
Bangalore, India, 2005
European Meeting of the Society for Free Radical Research, West Midlands, United Kingdom, 2005
International Workshop on Anthracycline Cardiotoxicity: Molecular Mechanisms and Clinical Correlates,
Como, Italy, 2006
Eighteenth Annual Meeting of the Korean Society for Molecular and Cellular Biology, Seoul, Korea, 2006
Medical Research Center for ROS, Kyung Hee University, Seoul, Korea, 2006
Bhabha Atomic Research Centre, Mumbai, India, 2007
Free Radicals in Montevideo, Uruguay, 2007
VIIth International Workshop on EPR in Biology and Medicine, Krakow, Poland, 2007
The Chemistry and Biology Reactive Oxygen Species, Wenner-Grenn Foundations International Symposium,
Stockholm, 2010
Departamento de Bioquímica, Universidad de la República, Montevideo, Uruguay, 2010
Biochemical Society, Birmingham, UK, 2011
6th Annual Spin Conference, Marseilles, France, 2011
Molecular and Cellular Bases of Redox Signaling and Oxidative Stress: Implications in Biomedicine, Baeza,
Spain (2011), 2011
1st Annual Conference of SMRM on Mitochondria in Biology and Medicine, Hyderabad, India, 2011
Free Radicals in Brazil 2011, VII Meeting of the SFRBM South American Group, São Pedro, Brazil, 2011
IXth International Workshop on EPR in Biology and Medicine, Krakow, Poland, 2013
Avidadham '13: International Conference on Innovations in Molecular Therapeutics, Centre for
Biotechnology, Anna University, Chennai, India, 2013
Zing Conference on Oxidative Stress, Oropesa, Spain, 2014
First Adriatic Symposium on Biophysical Approaches in Biomedical Studies, Split, Croatia, 2014
Societies for Free Radical Research - Australasia and Japan Conference, University of Otago, Christchurch,
New Zealand, 2015
Avidadham '15: International Conference on Innovations in Molecular Therapeutics, Centre for
Biotechnology, Anna University, Chennai, India, 2015
Winter School, Zakopane, Poland, 2015
COMMITTEE SERVICE:
Medical College
1989 Member, Graduate Program Committee, Medical College of Wisconsin
2001 Member, Search Committee for Chair of Biochemistry, Medical College of Wisconsin
2003 Member, Medical Scientist Training Program (MSTP) Admissions Committee, Medical College of
Wisconsin
2003 Member, Search Committee for Chair of Biochemistry, Medical College of Wisconsin
2007 Member, Technology Innovation Council, Medical College of Wisconsin
2008 Member, Search Committee for Director of Cancer Center, Medical College of Wisconsin
2008 Member, Search Committee for Associate Director of the CMCRT (Center for Medical
Countermeasures against Radiological Terrorism), Medical College of Wisconsin
2009 Member, Search Committee for Chair of Biochemistry, Medical College of Wisconsin
2009 - 2011 Chair, Biomedical Resource Center (BRC) Advisory Committee, Medical College of
Wisconsin
2010 Member, Search Committee for Chair of Radiology, Medical College of Wisconsin
2010 Member/Core Mentor, Clinical and Translational Science Institute (CTSI) Core Mentoring Group
Program, Medical College of Wisconsin
2010 Member, Leadership Committee, Cancer Center, Medical College of Wisconsin
2011 Member, Search Committee for Joan A. Van Deuren Chair in Breast Cancer Research, Cancer
Center, Medical College of Wisconsin
2011 Member, Women’s Health Research Program (WHRP) Steering Committee, Medical College of
Wisconsin
2012 Member, Community Medical Education Committee, Medical College of Wisconsin
2012 Member, Technology Evaluation Committee, Technology Innovation Council, Medical College of
Wisconsin
2013 Member, Search Committee for Chair of Radiology, Medical College of Wisconsin
2014 Member, Recruitment Committee for Wisconsin Breast Cancer Showhouse (WBCS) Chair, Cancer
Center, Medical College of Wisconsin
2015 Member, Search Committee for Chair of Anesthesiology, Medical College of Wisconsin
MEDICAL COLLEGE TEACHING ACTIVITIES:
Medical Student Education
2012 - 2015 Teaching “Active Oxygen & Oxidative Stress” in the Molecules to Cells course for M-1
students
Graduate Student Education
1986 Lectured in “Free Radical” Course for graduate students in Biophysics Graduate Program
1987 Lectured in “Reactive Metabolites” course for graduate students in Pharmacology & Toxicology
Department
1987 Course Director, Biophysics Graduate Seminar
1988 Lectured in “Oxygen Metabolism” course for graduate students in Biochemistry Department
1989 Taught “Electron Spin Resonance Spectroscopy” course for students in Biophysics Graduate Program
1992 Taught “Electron Spin Resonance Spectroscopy of Free Radicals” for students in Biophysics
Graduate Program
1993 Organized the Nitric Oxide journal club during the spring and fall semesters
1994 Taught “Free Radicals and ESR” for students in Biophysics Graduate Program
1996 Taught “ESR of Free Radicals in Biological Systems”
1999 Taught graduate course on “Free Radicals in Biology”
MCW STUDENTS, FACULTY, RESIDENTS AND CLINICAL/RESEARCH FELLOWS MENTORED:
Graduate Students
Committees
Jana Narasimhan, Medical College of Wisconsin, 1992 Biophysics
Gary Bachowski, Medical College of Wisconsin, 1993 Biochemistry
Netanya Spencer, Medical College of Wisconsin, 2003 Biophysics
Lynda Ludwig, Medical College of Wisconsin, 2004 Anesthesiology
Katarzyna Broniowska, Medical College of Wisconsin, 2008 Biophysics
Douglas Prah, Medical College of Wisconsin, 2008 Biophysics
Timothy Flewelen, Medical College of Wisconsin, 2012 Biophysics
PhD Students Advised
Eric J. Ferguson, Medical College of Wisconsin, 1999 “The Homocysteine Thiolactone Adduct of
Low-Density Lipoprotein”
Steven P. A. Goss, Medical College of Wisconsin, 1999 “The Antioxidant Effect of Nitric Oxide on
Low-Density Lipoprotein”
Hongtao Zhao, Medical College of Wisconsin, 2005 : “Detection of Reactive Oxygen and Nitrogen
Species in Biological Systems”
Tiesong Shang, Medical College of Wisconsin, 2005 “Mitochondria, ROS and Chemically-induced
Parkinsonism”
PhD Committees
Yongik Lee, Medical College of Wisconsin, 2016 Pharmacology and Toxicology
Postdoctoral Students
Witold Korytowski, Medical College of Wisconsin, 1983 - 1992
Barbara Pilas, Medical College of Wisconsin, 1985
Pavle Premovic, Medical College of Wisconsin, 1986 - 1987
Pei-Gen Sheng, Medical College of Wisconsin, 1988 - 1989
Ravinder Singh, Medical College of Wisconsin, 1989 - 1998
Sujatha Ramanujam, Medical College of Wisconsin, 1991 - 1992
Eugene Konorev, Medical College of Wisconsin, 1991 - 2002
Neil Hogg, Medical College of Wisconsin, 1993 - 1996
Hakim Karoui, Medical College of Wisconsin, 1994 - 1997
Srigiridhar Kotamraju, Medical College of Wisconsin, 1998 - 2003
Hao Zhang, Medical College of Wisconsin, 1998 - 2008
Kalpana Bhargava, Medical College of Wisconsin, 2000 - 2003
Shasi Kalivendi, Medical College of Wisconsin, 2000 - 2004
Govindaswami Shekar, Medical College of Wisconsin, 2000 - 2001
Yoshiko Tampo, Medical College of Wisconsin, 2001 - 2002
Suwei Wang, Medical College of Wisconsin, 2001 - 2003
Anuradha Cunnigaipur Dhanasekaran, Medical College of Wisconsin, 2002 - 2004
Karunakaran Chandran, Medical College of Wisconsin, 2002 - 2004
Toshiyuki Matsunaga, Medical College of Wisconsin, 2003 - 2004
Jacek Zielonka, Medical College of Wisconsin, 2004 - Present
Suresh Kumar, Medical College of Wisconsin, 2004 - 2007
Yingkai Xu, Medical College of Wisconsin, 2004 - 2006
Simmy Thomas, Medical College of Wisconsin, 2004 - 2005
Sravan Vanamala, Medical College of Wisconsin, 2005 - 2007
Deepika Aggarwal, Medical College of Wisconsin, 2005 - 2007
Marcos Lopez, Medical College of Wisconsin, 2006 - 2009
Micael Hardy, Medical College of Wisconsin, 2006 - 2008
Karunakaran Chandran, Medical College of Wisconsin, 2006 - 2009
Nir Weizman, Medical College of Wisconsin, 2007 - 2008
Adam Sikora, Medical College of Wisconsin, 2008 - 2009
Jin-Hwan Kim, Medical College of Wisconsin, 2009 - 2010
Jayapradha Sadaiyan Ramadoss, Medical College of Wisconsin, 2009 - 2010
Gang Cheng, Medical College of Wisconsin, 2009 - Present
Brian Dranka, Medical College of Wisconsin, 2010 - 2013
Radoslaw (Radek) Michalski, Medical College of Wisconsin, 2011 - 2012
Teruaki Koto, Medical College of Wisconsin, 2012
Michael Mouradian, Medical College of Wisconsin, 2014 - 2015
Radoslaw Podsiadly, Medical College of Wisconsin, 2014 - 2015
Asha Bailey, Medical College of Wisconsin, 2015 Diversity Summer Health-related Research Education
Program (DSHREP) Students
Marisol Madrigal, Medical College of Wisconsin, 2016 Diversity Summer Health-related Research
Education Program (DSHREP) Students
EXTRAMURAL TEACHING:
2016 Teaching “Targeting Cancer Metabolism” course to graduate students, Anna University, Chennai, India
through the Global Initiatives of Academic Netowrk (GIAN) program, an initiative of Ministry of Human
Resource Development, Government of India
GLOBAL HEALTH ACTIVITIES:
2014 Co-Chair, First Adriatic Symposium on Biophysical Applications in Biomedical Studies, August 24-28,
2014, Split, Croatia
2016 Teaching “Targeting Cancer Metabolism” course to graduate students, Anna University, Chennai, India
through the Global Initiatives of Academic Netowrk (GIAN) program, an initiative of Ministry of Human
Resource Development, Government of India
OTHER APPOINTMENTS:
1972 - 1978 Technical Development Chemist, CIBA-GEIGY of India, Ltd., Bombay, India
BIBLIOGRAPHY
Refereed Journal Publications/Original Papers
1. KALYANARAMAN, B., Atwood, J. L., and Kispert, L. D. Role of intermolecular hydrogen-bonding in the
crystal structure of alpha-chloroacetic acid. J. Chem. Soc. Chem. Comm. 715-16, 1976.
2. KALYANARAMAN, B., Kispert, L. D., and Atwood, J. L. The disordered crystal structure of
chlorodifluoroacetamide. J. Cryst. Mol. Struct. 6:311-6, 1976.
3. KALYANARAMAN, B., Kispert, L. D., and Atwood, J. L. The disordered crystal structure of
bromodifluoroacetamide and trifluoroacetamide. Acta Cryst. B34:1131-6, 1978.
4. KALYANARAMAN, B., Kispert, L. D., and Atwood, J. L. Crystal structure of 2-chloroacetamide (alpha-form):
a reinvestigation. J. Cryst. Mol. Struct. 8:175, 1978.
5. KALYANARAMAN, B. and Kispert, L. D. The ESR study of CF3--F radical in irradiated trifluoroacetamide
single crystals. J. Chem. Phys. 68:5219-24, 1978.
6. KALYANARAMAN, B. and Kispert, L. D. The ESR and INDO study of an oxygen centered radical in
irradiated 2-chloroacetamide. J. Chem. Phys. 68:5331-5, 1978.
7. KALYANARAMAN, B., Perez-Reyes, E., Mason, R. P., Peterson, F. J., and Holtzman, J. L. Electron spin
resonance evidence for a free radical intermediate in the cis-trans isomerizetion of furylfuramide by oxygensensitive nitroreductases. Mol. Pharmacol. 16:1059-64, 1979.
8. KALYANARAMAN, B., Perez-Reyes, E., and Mason, R. P. The reduction of nitroso-spin traps in chemical and
biological systems. A cautionary note. Tetrahedron Lett. 5:4809-12, 1979.
9. KALYANARAMAN, B., Mason, R. P., Perez-Reyes, E., Chignell, C. F., Wolf, C. R., and Philpot, R. M.
Characterization of the free radical formed in aerobic microsomal incubations containing carbon tetrachloride and
NADPH. Biochem. Biophys. Res. Commun. 89:1065-72, 1979.
10. Perez-Reyes, E., KALYANARAMAN, B., and Mason, R. P. The reductive metabolism of metronidazole and
ronidazole by aerobic liver microsomes. Mol. Pharmacol. 17:239-44, 1980.
11. KALYANARAMAN, B., Perez-Reyes, E., and Mason, R. P. Spin-trapping and direct electron spin resonance
investigations of the redox metabolism of quinone anticancer drugs. Biochim. Biophys. Acta 630:119-30, 1980.
12. Mason, R. P., KALYANARAMAN, B., Tainer, B., and Eling, T. E. A carbon-centered free radical intermediate
in the prostaglandin synthetase oxidation of arachidonic acid. Spin trapping and oxygen uptake studies. J. Biol.
Chem. 255:5019-22, 1980.
13. Wolf, C. R., Harrelson, W. G. Jr., Nastainczyk, W. M., Philpot, R. M., KALYANARAMAN, B., and Mason,
R. P. Metabolism of carbon tetrachloride in hepatic microsomes and reconstituted monooxygenase systems and its
relationship to lipid peroxidation. Mol. Pharmacol. 18:553-8, 1980.
14. Chignell, C. F., KALYANARAMAN, B., Mason, R. P., and Sik, R. H. Spectroscopic studies of cutaneous
photosensitizing agents. I. Spin trapping of photolysis products from sulfanilamide, 4-aminobenzoic acid and
related compounds. Photochem. Photobiol. 32:563, 1980.
15. Chignell, C. F., KALYANARAMAN, B., Sik, R. H., and Mason, R. P. Spectroscopic studies of cutaneous
photosensitizing agents. II. Spin trapping of photolysis products from sulfanilamide and 4-aminobenzoic acid using
5,5-dimethyl-1-pyrroline-1-oxide (DMPO). Photochem. Photobiol. 34:147, 1981.
16. Roger, R. D., KALYANARAMAN, B., Dalton, S. M., Smith, W., Kispert, L. D., and Atwood, J. L. Crystal
structure of bromofluoroacetic acid: a chiral molecule. J. Cryst. Mol. Struct. 11:104, 1981.
17. Lasker, J. M., Sivarajah, K., Mason, R. P., KALYANARAMAN, B., Abou-Donia, M. B., and Eling, T.E. A
free radical mechanism of prostaglandin synthetase-dependent aminopyrine demethylation. J. Biol. Chem. 256,
7764-7, 1981.
18. KALYANARAMAN, B., Mason, R. P., Rowlett, R., and Kispert, L. D. An electron spin resonance
investigation and molecular orbital calculation of the anion radical intermediate in the enzymatic cis-trans
isomerization of furylfuramide, a nitrofuran derivative of ethylene. Biochim. Biophys. Acta 660:102-9, 1981.
19. Mottley, C., KALYANARAMAN, B., and Mason, R. P. Spin trapping artifacts due to the reduction of nitroso
spin traps. FEBS Lett. 130:12, 1981.
20. KALYANARAMAN, B., Mason, R. P., Tainer, B., and Eling, T. E. The free radical formed during the
hydroperoxide-mediated deactivation of ram seminal vesicles is hemoprotein-derived. J. Biol. Chem. 257:4764-8,
1982.
21. KALYANARAMAN, B., Mason, R. P., and Sivarajah, K. An electron spin resonance study of the novel radical
cation produced during the horseradish peroxidase-catalyzed oxidation of tetramethylhydrazine. Biochem. Biophys.
Res. Commun. 105:217-24, 1982.
22. KALYANARAMAN, B. and Sealy, R. C. Electron spin resonance--spin stabilization in enzymatic systems:
detection of semiquinones produced during peroxidatic oxidation of catechols and catecholamines. Biochem.
Biophys. Res. Commun. 106:1119-25, 1982.
23. KALYANARAMAN, B., Felix, C. C., and Sealy, R. C. Photoionization of melanin precursors: An electron
spin resonance investigation using the spin trap 5,5-dimethyl-1-pyrroline-1-oxide (DMPO). Photochem. Photobiol.
36:5, 1982.
24. KALYANARAMAN, B., Sivarajah, K., Eling, T. E., and Mason, R. P. A free radical mediated cooxidation of
tetramethylhydrazine by prostaglandin hydroperoxidase. Carcinogenesis 4:1341-3, 1983.
25. KALYANARAMAN, B., Mottley, C., and Mason, R. P. A direct electron spin resonance and spin-trapping
investigation of peroxyl free radical formation by hematin/hydroperoxide systems. J. Biol. Chem. 258:3855-8,
1983.
26. Sinha, B. K., Trush, M. A., and KALYANARAMAN, B. Free radical metabolism of VP-16 and inhibition of
anthracycline-induced lipid peroxidation. Biochem. Pharmacol. 32:3495-8, 1983.
27. Samskog, P. O., Kispert, L. D., and KALYANARAMAN, B. The structure of the radical anion in an irradiated
iodoacetamide: an EPR and INDO study. J.C.S. Faraday Trans. 2 80:267, 1984.
28. KALYANARAMAN, B., Mottley, C., and Mason, R. P. On the use of organic extrac-tion in the spin-trapping
technique as applied to biological systems. J. Biochem. Biophys. Methods 9:27-31, 1984.
29. KALYANARAMAN, B., Felix, C. C., and Sealy, R. C. Electron spin resonance-spin stabilization of
semiquinones produced during oxidation of epinephrine and its analogues. J. Biol. Chem. 259:354-8, 1984.
30. KALYANARAMAN, B., Sealy, R. C., and Sinha, B. K. An electron spin resonance study of the reduction of
peroxides by anthracycline semiquinones. Biochim. Biophys. Acta 799:270-5, 1984.
31. KALYANARAMAN, B., Felix, C. C., and Sealy, R. C. Peroxidatic oxidation of catecholamines. A kinetic
electron spin resonance investigation using the spin stabilization approach. J. Biol. Chem. 259:7584-9, 1984.
32. Basosi, R., Sealy, R. C., KALYANARAMAN, B., and Hyde, J. S. Interaction of semiquinones with
paramagnetic metal ions in aqueous solution: relaxation and complex formation. J. Magn. Reson. 59:41, 1984.
33. Sealy, R. C., Puzyna, W., KALYANARAMAN, B., and Felix, C. C. Identification by electron spin resonance
spectroscopy of free radicals produced during autoxidative melanogenesis. Biochim. Biophys. Acta 800:269-76,
1984.
34. KALYANARAMAN, B., Sealy, R. C., and Sivarajah, K. An electron spin resonance study of o-semiquinones
formed during the enzymatic and autoxidation of catechol estrogens. J. Biol. Chem. 259:14018-22, 1984.
35. KALYANARAMAN, B., Felix, C. C., and Sealy, R. C. Photoionization and photohomo¬lysis of eumelanins:
an electron spin resonance - spin trapping study. J. Am. Chem. Soc. 106:7327, 1984.
36. Antholine, W. E., Sarna, T., Sealy, R. C., KALYANARAMAN, B., Shields, G. D., and Petering, D. H. Free
radicals from the photodecomposition of bleomycin. Photochem. Photobiol. 41, 393-9, 1985.
37. KALYANARAMAN, B. and Sohnle, P. G. Generation of free radical intermediates from foreign compounds
by neutrophil-derived oxidants. J. Clin. Invest. 75:1618-22, 1985.
38. Prabhananda, B. S., KALYANARAMAN, B., and Sealy, R. C. Radical anions from one-electron-reduced
adrenochrome. Detection and identification by electron spin resonance spectroscopy. Biochim. Biophys. Acta
840:21-8, 1985.
39. KALYANARAMAN, B., Janzen, E. G., and Mason, R. P. Spin trapping of the azidyl radical in
azide/catalase/H2O2 and various azide/peroxidase/H2O2 peroxidizing systems. J. Biol. Chem. 260, 4003-6, 1985.
40. Sinha, B. K., Trush, M. A., and KALYANARAMAN, B. Microsomal interactions and inhibition of lipid
peroxidation by etoposide (VP-16, 213): implications for mode of action. Biochem. Pharmacol. 34:2036-40, 1985.
41. Sinha, B. K., Strong, J., Gibson, N. W., and KALYANARAMAN, B. Mechanism of DNA strand breaks by
mitonafide, an imide derivative of 3-nitro-1,8-naphthalic acid. Biochem. Pharmacol. 34:3845-52, 1985.
42. Korytowski, W., Hintz, P., Sealy, R. C., and KALYANARAMAN, B. Mechanism of dismutation of superoxide
produced during autoxidation of melanin pigments. Biochem. Biophys. Res. Commun. 131:659-65, 1985.
43. Kersten, P. J., Tien, M., KALYANARAMAN, B., and Kirk, T. K. The ligninase of Phanero¬chaete
chrysosporium generates cation radicals from methoxybenzenes. J. Biol. Chem. 260:2609-12, 1985.
44. Hammel, K. E., Tien, M., KALYANARAMAN, B., and Kirk, T. K. Mechanism of oxidative C alpha-C beta
cleavage of a lignin model dimer by Phanerochaete chrysosporium ligninase. Stoichiometry and involvement of
free radicals. J. Biol. Chem. 260:8348-53, 1985.
45. Kirk, T. K., Tien, M., Kersten, P. J., Mozuch, M. D., and KALYANARAMAN, B. Ligninase of Phanerochaete
chrysosporium. Mechanism of its degradation of the non-phenolic arylglycerol-beta-aryl ether substructure of
lignin. Biochem. J. 236:279-87, 1986.
46. Hammel, K. E., KALYANARAMAN, B., and Kirk, T. K. Substrate free radicals are intermediates in ligninase
catalysis. Proc. Natl. Acad. Sci. USA 83:3708-12, 1986.
47. Korytowski, W., KALYANARAMAN, B., Menon, I. A., Sarna, T., and Sealy, R. C. Reaction of superoxide
anions with melanins: electron spin resonance and spin trapping studies. Biochim. Biophys. Acta 882:145-53,
1986.
48. Hintz, P. and KALYANARAMAN, B. Metal ion-induced activation of molecular oxygen in pigmented
polymers. Biochim. Biophys. Acta 883:41-5, 1986.
49. Pilas, B., Felix, C. C., Sarna, T., and KALYANARAMAN, B. Photolysis of pheomelanin precursors: an ESRspin trapping study. Photochem. Photobiol. 44:689-96, 1986.
50. Ankel, E., Felix, C. C., and KALYANARAMAN, B. The use of spin label oximetry in the study of
photodynamic inactivation of Chinese hamster ovary cells. Photochem. Photobiol. 44:741-6, 1986.
51. Hammel, K. E., KALYANARAMAN, B., and Kirk, T. K. Oxidation of polycyclic aromatic hydrocarbons and
dibenzo[p]-dioxins by Phanerochaete chrysosporium ligninase. J. Biol. Chem. 261:16948-52, 1986.
52. Korytowski, W., Sarna, T., KALYANARAMAN, B., and Sealy, R. C. Tyrosinase-catalyzed oxidation of dopa
and related catechol(amine)s: a kinetic electron spin resonance investigation using spin-stabilization and spin label
oximetry. Biochim. Biophys. Acta 924:383-92, 1987 [Erratum: Biochim. Biophys. Acta 926:203, 1987].
53. Schreiber, J., Mottley, C., Sinha, B. K., KALYANARAMAN, B., and Mason, R. P. One-electron reduction of
daunomycin, daunomycinone, and 7-deoxydaunomy¬cinone by the xanthine/xanthine oxidase system: Detection of
semiquinone free radicals by electron spin resonance. J. Am. Chem. Soc. 109:348, 1987.
54. Katki, A. G., KALYANARAMAN, B., and Sinha, B. K. Interactions of the antitumor drug, etoposide, with
reduced thiols in vitro and in vivo. Chem. Biol. Interact. 62:237-47, 1987.
55. Korytowski, W., Felix, C. C., and KALYANARAMAN, B. Mechanism of oxidation of 1-methyl-4-phenyl-2,3dihydropyridinium (MPDP+). Biochem. Biophys. Res. Commun. 144:692-8, 1987.
56. Korytowski, W., Felix, C. C., and KALYANARAMAN, B. Evidence for the one-electron oxidation of 1methyl-4-phenyl-2,3-dihydropyridinium (MPDP+). Biochem. Biophys. Res. Commun. 147:354-60, 1987.
57. KALYANARAMAN, B., Premovic, P. I., and Sealy, R. C. Semiquinone anion radicals from addition of amino
acids, peptides, and proteins to quinones derived from oxidation of catechols and catecholamines. An ESR spin
stabilization study. J. Biol. Chem. 262:11080-7, 1987.
58. Korytowski, W., Pilas, B., Sarna, T., and KALYANARAMAN, B. Photoinduced generation of hydrogen
peroxide and hydroxyl radicals in melanins. Photochem. Photobiol. 45:185-90, 1987.
59. KALYANARAMAN, B., Feix, J. B., Sieber, F., Thomas, J. P., and Girotti, A. W. Photodynamic action of
merocyanine 540 on artificial and natural cell membranes: involvement of singlet molecular oxygen. Proc. Natl.
Acad. Sci. USA 84:2999-3003, 1987.
60. Menon, I. A., Basu, P. K., Persad, S., Avaria, M., Felix, C. C., and KALYANARAMAN, B. Is there any
difference in the photobiological properties of melanins isolated from human blue and brown eyes? Br. J.
Ophthalmol. 71:549-52, 1987.
61. Korytowski, W., Felix, C. C., and KALYANARAMAN, B. Oxygen activation during the interaction between
MPTP metabolites and synthetic neuromelanin--an ESR-spin trapping, optical, and oxidase electrode study.
Biochem. Biophys. Res. Commun. 154:781-8, 1988.
62. KALYANARAMAN, B., Korytowski, W., Pilas, B., Sarna, T., Land, E. J., and Truscott, T. G. Reaction
between ortho-semiquinones and oxygen: pulse radiolysis, electron spin resonance, and oxygen uptake studies.
Arch. Biochem. Biophys. 266:277-84, 1988.
63. Prabhananda, B. S., Felix, C. C., KALYANARAMAN, B., and Felix, C. C. Free radicals formed from the
oxidation of 4-aminocatechol. Identification and structural dynamics using ESR spectroscopy. J. Magn. Reson.
76:264, 1988.
64. Sinha, B. K., Eliot, H. M., and KALYANARAMAN, B. Iron-dependent hydroxyl radical formation and DNA
damage from a novel metabolite of the clinically active antitumor drug VP-16. FEBS Lett. 227:240-4, 1988.
65. Bernofsky, C., Olavesen, Y. E., Felix, C. C., and KALYANARAMAN, B. Evidence for a hydroperoxyl radical
from hypochlorite-modified adenosine 5'-monophosphate (AMP). Biochem. Arch. 4:103-7, 1988.
66. Pilas, B., Sarna, T., KALYANARAMAN, B., and Swartz, H. M. The effect of melanin on iron associated
decomposition of hydrogen peroxide. Free Radic. Biol. Med. 4:285-93, 1988.
67. Feix, J. B., KALYANARAMAN, B., Chignell, C. F., and Hall, R. D. Direct observation of singlet oxygen
production by merocyanine 540 associated with phosphatidylcholine liposomes. J. Biol. Chem. 263:17247-50,
1988.
68. Hodnick, W. F., KALYANARAMAN, B., Pritsos, C. A., and Pardini, R. S. The production of hydroxyl and
semiquinone free radicals during the autoxidation of redox active flavonoids. Basic Life Sci. 49:149-52, 1988.
69. KALYANARAMAN, B., Korytowski, W., and Felix, C. C. Mechanism of oxidation of 1-methyl-4-phenyl-3,3dihydropyridinium (MPDP+). Basic Life Sci. 49:781-5, 1988.
70. Odier, E., Mozuch, M. D., KALYANARAMAN, B., and Kirk, T. K. Ligninase-mediated phenoxy radical
formation and polymerization unaffected by cellobiose: quinone oxidoreductase. Biochimie 70:847-52, 1988
[Erratum: Biochimie 70:1126, 1988].
71. Baker, J. E., Felix, C. C., Olinger, G. N., and KALYANARAMAN, B. Myocardial ischemia and reperfusion:
direct evidence for free radical generation by electron spin resonance spectroscopy. Proc. Natl. Acad. Sci. USA
85:2786-9, 1988.
72. Baker, J. E. and KALYANARAMAN, B. Ischemia-induced changes in myocardial paramagnetic metabolites:
implications for intracellular oxy-radical generation. FEBS Lett. 244:311-4, 1989.
73. Feix, J. B. and KALYANARAMAN, B. Spin trapping of lipid-derived radicals in liposomes. Biochim.
Biophys. Acta 992:230-5, 1989.
74. KALYANARAMAN, B., Sealy, R. C., and Liehr, J. G. Characterization of semiquinone free radicals formed
from stilbene catechol estrogens. An ESR spin stabilization and spin trapping study. J. Biol. Chem. 264:11014-9,
1989.
75. KALYANARAMAN, B., Nemec, J., and Sinha, B. K. Characterization of free radicals produced during
oxidation of etoposide (VP-16) and its catechol and quinone derivatives. An ESR study. Biochemistry 28:4839-46,
1989.
76. Athar, M., Mukhtar, H., Bickers, D. R., Kahn, I. U., and KALYANARAMAN, B. Evidence for the metabolism
of tumor promoter organic hydroperoxides into free radicals by human carcinoma skin keratinocytes: an ESR-spin
trapping study. Carcinogenesis 10:1499-503, 1989.
77. Pieper, G. M., Gross, G. J., and KALYANARMAN, B. An ESR study of the nitroxide radical of pentastarchconjugated deferoxamine. Free Radic. Biol. Med. 9:211-8,1990.
78. Sinha, B. K., Antholine, W. E., KALYANARAMAN, B., and Eliot, H. M. Copper ion-dependent oxy-radical
mediated DNA damage from dihydroxy derivative of etoposide. Biochim. Biophys. Acta 1096:81-3, 1990.
79. Sheng, P. G., Feix, J. B., and KALYANARAMAN, B. Characterization of radical adducts formed during
photochemical spin trapping in liposomes. Photochem. Photobiol. 52:323-31 (1990).
80. Kersten, P. J., KALYANARAMAN, B., Hammel, K. E., Reinhammar, B., and Kirk, T. K. Comparison of
lignin peroxidase, horseradish peroxidase and laccase in the oxidation of methoxybenzenes. Biochem. J. 268:47580, 1990.
81. Popp, J. L., KALYANARAMAN, B., and Kirk, T. K. Lignin peroxidase oxidation of Mn2+ in the presence of
veratryl alcohol, malonic or oxalic acid, and oxygen. Biochemistry 29:10475-80, 1990.
82. KALYANARAMAN, B., Antholine, W. E., and Parthasarathy, S. Oxidation of low-density lipoprotein by
Cu2+ and lipoxygenase: an electron spin resonance study. Biochim. Biophys. Acta 1035:286-92, 1990.
83. KALYANARAMAN, B. and Baker, J. E. On the detection of paramagnetic species in the adriamycin-perfused
rat heart: a reappraisal. Biochem. Biophys. Res. Commun. 169:30-8, 1990.
84. Kalyanarman, B., Morehouse, K. M., and Mason, R. P. An electron paramagnetic resonance study of the
interactions between the adriamycin semiquinone, hydrogen peroxide, iron-chelators, and radical scavengers. Arch.
Biochem. Biophys. 286:164-70, 1991.
85. Antholine, W. E., KALYANARAMAN, B., Templin, J. A., Brynes, R. W., and Petering, D. H. Spin-trapping
studies of the oxidation-reduction reactions of iron bleomycin in the presence of thiols and buffer. Free Radic. Biol.
Med. 10:119-23, 1991.
86. Roy, D., KALYANARAMAN, B., and Liehr, J. G. Xanthine oxidase-catalyzed reduction of estrogen quinones
to semiquinones and hydroquinones. Biochem. Pharmacol. 42:1627-31, 1991.
87. Feix, J. B. and KALYANARAMAN, B. An electron spin resonance study of merocyanine 540-mediated type I
reactions in liposomes. Photochem. Photobiol. 53:39-45, 1991.
88. Singh, R. J., Feix, J. B., Pintar, T. J., Girotti, A. W., and KALYANARAMAN, B. Photodynamic action of
merocyanine 540 in artificial bilayers and natural membranes: action spectra and quantum yields. Photochem.
Photobiol. 53:493-500, 1991.
89. Feix, J. B. and KALYANARAMAN, B. Production of singlet oxygen-derived hydroxyl radical adducts during
merocyanine 540-mediated photosensitization: analysis by ESR-spin trapping and HPLC with electrochemical
detection. Arch. Biochem. Biophys. 291:43-51, 1991.
90. Feix, J. B. and KALYANARAMAN, B. An electron spin resonance study of merocy-anine 540-mediated type I
reactions in liposomes. Photochem. Photobiol. 53:39-45, 1991.
91. O'Brien, J. M., Singh, R. J., Feix, J. B., KALYANARAMAN, B., and Sieber, F. Action spectra of the
antileukemic and antiviral activities of merocyanine 540. Photochem. Photobiol. 54:851-4, 1991.
92. Menon, I. A., Persad, S. D., Haberman, H. F., Basu, P. K., Norfray, J. F., Felix, C. C., and
KALYANARAMAN, B. Characterization of the pigment from homogentisic acid and urine and tissue from an
alkaptonuria patient. Biochem. Cell Biol. 69:269-73, 1991.
93. KALYANARAMAN, B., Parthasarathy, S., Joseph, J., and Froncisz, W. EPR spectra in a loop-gap resonator
for a spin-trapped radical from a low-density lipoprotein. J. Magn. Reson. 92:342-7, 1991.
94. Towell, J. and KALYANARAMAN, B. Detection of radical adducts of 5,5-dimethyl-1-pyrroline n-oxide by the
combined use of high-performance liquid chromatography with electrochemical detection and electron spin
resonance. Anal. Biochem. 196:111-9, 1991.
95. Maruyama, M., Pieper, G. M., KALYANARAMAN, B., Hallaway, P. E., Hedlund, B. E., and Gross, G. J.
Effects of hydroxyethyl starch conjugated deferoxamine on myocardial functional recovery following coronary
occlusion and reperfusion in dogs. J. Cardiovasc. Pharmacol. 17:166-75, 1991.
96. KALYANARAMAN, B., Joseph, S., and Parthasarathy, S. The spin trap, alpha-phenyl N-tert-butylnitrone,
inhibits the oxidative modification of low density lipoprotein. FEBS Lett. 280:17-20, 1991.
97. KALYANARAMAN, B., Joseph, J., Kondratenko, N., and Parthasarathy, S. Direct modification of low density
lipoprotein by the spin trap 3,5-dibromo-4-nitrosobenzene sulfonic acid. Biochim. Biophys. Acta 1126:309-13,
1992.
98. KALYANARAMAN, B., Darley-Usmar, V. M., Wood, J., Joseph, J., and Parthasarathy, S. Synergistic
interaction between the probucol phenoxyl radical and ascorbic acid in inhibiting the oxidation of low density
lipoprotein. J. Biol. Chem. 267:6789-95, 1992.
99. Sugumaran, M., Semensi, V., KALYANARAMAN, B., Bruce, J. M., and Land, E. J. Evidence for the
formation of a quinone methide during the oxidation of insect cuticular sclerotizing precusor 1,2-dehydro-Nacetyldopamine. J. Biol. Chem. 267:10355-61, 1992.
100. Singh, R. J., Feix, J. B., and KALYANARAMAN, B. Photobleaching of merocyanine 540: involvement of
singlet molecular oxygen. Photochem. Photobiol. 55:483-9, 1992.
101. Anderson, M. S., KALYANARAMAN, B., and Feix, J. B. Enhancement of merocyanine 540-mediated
phototherapy by salicylate. Cancer Res. 53:806-9, 1993.
102. KALYANARAMAN, B., Ramanujam, S., Singh, R. J., and Feix, J. B. Formation of 2,5-dihydroxybenzoic
acid during the reaction between 1O2 and salicylic acid: Analysis by ESR oximetry and HPLC with
electrochemical detection. J. Am. Chem. Soc. 115:4007-12, 1993.
103. Konorev, E. A., Baker, J. E., Joseph, J., and KALYANARAMAN, B. Vasodilatory and toxic effects of spin
traps on aerobic cardiac function. Free Radic. Biol. Med. 14:127-37, 1993.
104. KALYANARAMAN, B., Joseph, J., and Parthasarathy, S. Site-specific trapping of reactive species in lowdensity lipoprotein oxidation: biological implications. Biochim. Biophys. Acta 1168:220-7, 1993.
105. KALYANARAMAN, B., Joseph, J., and Parthasarathy, S. The use of spin traps to investigate site-specific
formation of free radicals in low-density lipoprotein oxidation. Biochem. Soc. Trans. 21:318-21, 1993. Review.
106. Joseph, J., KALYANARAMAN, B., and Hyde, J. S. Trapping of nitric oxide by nitronyl nitroxides: an
electron spin resonance investigation. Biochem. Biophys. Res. Commun. 192:926-34, 1993.
107. Aust, S. D., Chignell, C. F., Bray, T. M., KALYANARAMAN, B., and Mason, R. P. Free radicals in
toxicology. Toxicol. Appl. Pharmacol. 120:168-78, 1993. Review.
108. Pace, M. D. and KALYANARAMAN, B. Spin trapping of nitrogen dioxide radical from photolytic
decomposition of nitramines. Free Radic. Biol. Med. 15, 337-42, 1993.
109. Konorev, E. A., Struck, A. T., Baker, J. E., Ramanujam, S., Thomas, J. P., Radi, R., and
KALYANARAMAN, B. Intracellular catalase inhibition does not predispose rat heart to ischemia-reperfusion and
hydrogen peroxide-induced injuries. Free Radic. Res. Commun. 19:397-407, 1993.
110. Graham, A., Hogg, N., KALYANARAMAN, B., O'Leary, V., Darley-Usmar, V., and Moncada, S.
Peroxynitiric modification of low-density lipoprotein leads to recognition by the macrophage scavenger receptor.
FEBS Lett. 330:181-6, 1993.
111. Hogg, N., KALYANARAMAN, B., Joseph, J., Struck, A., and Parthasarathy, S. Inhibition of low-density
lipoprotein oxidatin by nitric oxide. Potential role in atherogenesis. FEBS Lett. 334:170-4, 1993.
112. Baker, J. E., Konorev, E. A., Tse, S. Y., Joseph, J., and KALYANARAMAN, B. Lack of protection of PBN in
isolated heart during ischemia and reperfusion: implications for radical scavenging mechanism. Free Radic. Res.
20:145-63, 1994. [Erratum: Free Radic. Res. 20:411, 1994].
113. Thomas, C. E., Ku, G., and KALYANARAMAN, B. Nitrone spin trap lipophilicity as a determinant for
inhibition of low density lipoprotein oxidation and activation of interleukin-1 beta release from human monocytes.
J. Lipid Res. 35:610-9, 1994.
114. Feix, J. B., Anderson, M. S., and KALYANARAMAN, B. Enhancement of merocyanine 540 uptake and
photodynamic cell killing by salicylates. Cancer Res. 54:3474-8, 1994.
115. Singh, R. J., Hogg, N., Mchaourab, H. S., and KALYANARAMAN, B. Physical and chemical interactions
between nitric oxide and nitroxides. Biochim. Biophys. Acta 1201:437-41, 1994.
116. Hogg, N., Joseph, J., and KALYANARAMAN, B. The oxidation of alpha-tocopherol and trolox by
peroxynitrite. Arch. Biochem. Biophys. 314:153-8, 1994.
117. Thomas, J. P., KALYANARAMAN, B., and Girotti, A. W. Involvement of preexisting lipid hydroperoxides
in Cu2+-stimulated oxidation of low-density lipoprotein. Arch. Biochem. Biophys. 315:244-54, 1994.
118. Rubbo, H., Radi, R., Trujillo, M., KALYANARAMAN, B., Barnes, S., and Freeman, B. A. Nitric oxide
regulation of superoxide and peroxynitrite-dependent lipid peroxidation: formation of nitrogen-containing oxidized
lipid derivatives. J. Biol. Chem. 269:26066-75, 1994.
119. Thomas, C. E., Ohlweiler, D. F., and KALYANARAMAN, B. Multiple mechanisms for inhibition of low
density lipoprotein oxidation by novel cyclic nitrone spin traps. J. Biol. Chem. 269:28055-61, 1994.
120. Konorev, E. A., Tarpey, M. M., Joseph, J., Baker, J. E., and KALYANARAMAN, B. Nitronyl nitroxides as
probes to study the mechanism of vasodilatory action of nitrovasodilators, nitrone spin traps, and nitroxides: role of
nitric oxide. Free Radic. Biol. Med. 18:169-77, 1995.
121. Pieper, G. M., Felix, C. C., KALYANARAMAN, B., Turk, M., and Roza, A. M. Detection by ESR of DMPO
hydroxyl adduct formation from islets of Langerhans. Free Radic. Biol. Med. 19:219-25, 1995.
122. Hogg, N., Singh, R. J., Joseph, J., Neese, F., and KALYANARAMAN, B. Reactions of nitric oxide with
nitronyl nitroxides and oxygen: prediction of nitrite and nitrate formation by kinetic simulation. Free Radic. Res.
22:47-56, 1995.
123. Singh, R. J., Hogg, N., Neese, F., Joseph, J., and KALYANARAMAN, B. Trapping of nitric oxide formed
during photolysis of sodium nitroprusside in aqueous and lipid phases: an electron spin resonance study.
Photochem. Photobiol. 61:325-30 (1995).
124. KALYANARAMAN, B., Darley-Usmar, V., Struck, A., Hogg, N., and Parthasarathy, S. Role of
apolipoprotein B-derived radical and alpha-tocopheroxyl radical in peroxidase-dependent oxidation of low density
lipoprotein. J. Lipid Res. 36:1037-45, 1995.
125. Struck, A. T., Hogg, N., Thomas, J. P., and KALYANARAMAN, B. Nitric oxide donor compounds inhibit
the toxicity of oxidized low-density lipoprotein to endothelial cells. FEBS Lett. 361:291-4, 1995.
126. Singh, R. J., Hogg, N., Joseph, J., and KALYANARAMAN, B. Photosensitized decomposition of Snitrosothiols and 2-methyl-2-nitrosopropane: possible use for site-directed nitric oxide production. FEBS Lett.
360:47-51, 1995.
127. Rubbo, H., Parthasarathy, S., Barnes, S., Kirk, M, KALYANARAMAN, B., and Freeman, B. A. Nitric oxide
nitrosation of lipoxygenase-dependent liposomal and lipoprotein oxidation: formation of nitrogen-containing
oxidized lipid derivatives. J. Biol. Chem. 269:26066-75, 1995.
128. Singh, R. J., Feix, J. B., Mchaourab, H. S., Hogg, N., and KALYANARAMAN, B. Spin-labeling study of the
oxidative damage to low-density lipoprotein. Arch. Biochem. Biophys. 320:155-61, 1995.
129. Konorev, E. A., Tarpey, M. M., Joseph, J., Baker, J. E., and KALYANARAMAN, B. S-nitrosoglutathione
improves functional recovery in the isolated rat heart after cardioplegic ischemic arrest--evidence for a
cardioprotective effect of nitric oxide. J. Pharmacol. Exp. Ther. 274:200-6, 1995.
130. Hogg, N., Struck, A., Goss, S. P., Santanam, N., Joseph, J., Parthasarathy, S., and KALYANARAMAN, B.
Inhibition of macrophage-dependent low density lipoprotein oxidation by nitric-oxide donors. J. Lipid Res.
36:1756-62, 1995.
131. KALYANARAMAN, B., Konorev, E. A., Joseph, J., and Baker, J. E. Is free radical generation an important
component of ischemia/reperfusion injury? Part 2. Redox Report 1:181-4, 1995.
132. Goss, S. P., Hogg, N., and KALYANARAMAN, B. The antioxidant effect of spermine NONOate in human
low-density lipoprotein. Chem. Res. Toxciol. 8:800-6, 1995.
133. KALYANARAMAN, B. Radical intermediates during degradation of lignin-model compounds and
environmental pollutants: an electron spin resonance study. Xenobiotica 25:667-75, 1995. Review.
134. Rubbo, H., Parthasarathy, S., Barnes, S., Kirk, M., KALYANARAMAN, B., and Freeman, B. A. Nitric oxide
inhibition of lipoxygenase-dependent liposome and low-density lipoprotein oxidation: termination of radical chain
propagation reactions and formation of nitrogen-containing oxidized lipid derivatives. Arch. Biochem. Biophys.
324:15-25, 1995.
135. Singh, R. J., Hogg, N., and KALYANARAMAN, B. Interaction of nitric oxide with photoexcited rose bengal:
evidence for one-electron reduction of nitric oxide to nitroxyl anion. Arch. Biochem. Biophys. 324:367-73 (1995).
136. Konorev, E. A., Joseph, J., and KALYANARAMAN, B. S-nitrosoglutathione induces formation of
nitrosylmyoglobin in isolated hearts during cardioplegic ischemia--an electron spin resonance study. FEBS Lett.
378:111-4, 1996.
137. Hogg, N., Singh, R. J., and KALYANARAMAN, B. The role of glutathione in the transport and catabolism of
nitric oxide. FEBS Lett. 382:223-8, 1996.
138. Karoui, H., Hogg, N., Joseph, J., and KALYANARAMAN, B. Effect of superoxide dismutase mimics on
radical adduct formation during the reaction between peroxynitrite and thiols--an ESR-spin trapping study. Arch.
Biochem. Biophys. 330:115-24, 1996.
139. Karoui, H., Hogg, N., Frejaville, C., Tordo, P., and KALYANARAMAN, B. Characterization of sulfurcentered radical intermediates formed during the oxidation of thiols and sulfite by peroxynitrite: ESR-spin trapping
and oxygen uptake studies. J. Biol. Chem. 271:6000-9, 1996.
140. Konorev, E. A., Joseph, J., Tarpey, M. M., and KALYANARAMAN, B. The mechanism of cardioprotection
by S-nitrosoglutathione monoethyl ester in rat isolated heart during cardioplegic ischaemic arrest. Br. J. Pharmacol.
119:511-8, 1996.
141. Singh, R. J., Hogg, N., Joseph, J., and KALYANARAMAN, B. Mechanism of nitric oxide release from Snitrosothiols. J. Biol. Chem. 271:18596-603, 1996.
142. Hogg, N., Singh, R. J., Goss, S. P. A., and KALYANARAMAN, B. The reaction between nitric oxide and
alpha-tocopherol: a reappraisal. Biochem. Biophys. Res. Commun. 224:696-702, 1996.
143. KALYANARAMAN, B., Karoui, H., Singh, R. J., and Felix, C. C. Detection of thiyl radical adducts formed
during hydroxyl radical- and peroxynitrite-mediated oxidation of thiols--a high resolution ESR spin-trapping study
at Q-band (35 GHz). Anal. Biochem. 241:75-81, 1996.
144. Curtis, J. F., Reddy, N. G., Mason, R. P., KALYANARAMAN, B., and Eling, T. E. Nitric oxide: a
prostaglandin H synthase 1 and 2 reducing cosubstrate that does not stimulate cyclooxygenase activity or
prostaglandin H synthase expression in murine macrophages. Arch. Biochem. Biophys. 335:369-76, 1996.
145. Baker, J. E., Froncisz, W., Joseph, J., and KALYANARAMAN, B. Spin label oximetry to assess extracellular
oxygen consumption during myocardial ischemia. Free Radic. Biol. Med. 22:109-15, 1997.
146. Hogg, N., Singh, R. J., Konorev, E., Joseph, J., and KALYANARAMAN, B. S-Nitrosoglutathione as a
substrate for gamma-glutamyl transpeptidase. Biochem. J. 323:477-81, 1997.
147. V´asquez-Vivar, J., Hogg, N., Pritchard, K. A. Jr., Martasek, P., and KALYANARAMAN, B. Superoxide
anion formation from lucigenin: an electron spin resonance spin-trapping study. FEBS Lett. 403:127-30, 1997.
148. Ferguson, E., Singh, R. J., Hogg, N., and KALYANARAMAN, B. The mechanism of apolipoprotein B-100
thiol depletion during oxidative modification of low-density lipoprotein. Arch. Biochem. Biophys. 341:287-94,
1997.
149. Karoui, H., Hansert, B., Sand, P. J., Tordo, P., Bohle, D. S., and KALYANARAMAN, B. Spin trapping of
free radicals formed during the oxidation of glutathione by tetramethylammonium peroxynitrite. Nitric Oxide:
Biology and Chemistry 1:346-58, 1997.
150. Nakanishi, A. L., Roza, A. M., Adams, M. B., Seibel, R., Moore-Hilton, G., KALYANARAMAN, B., and
Pieper, G. M. Electron spin resonance analysis of heme-nitrosyl and reduced iron-sulfur centered complexes in
allogeneic, heterotopic cardiac transplants--effects of treatment with pyrrolidine dithiocarbamate. Free Radic. Biol.
Med. 25:201-7, 1998.
151. Goss, S. P. A., Hogg, N., and KALYANARAMAN, B. The effect of nitric oxide release rates on the oxidation
of human low-density lipoprotein. J. Biol. Chem. 272:21647-53, 1997.
152. Smalley, D.M., Hogg, N., KALYANARAMAN, B., and Pritchard, K. A. Jr. Endothelial cells prevent
accumulation of lipid hydroperoxides in low-density lipoprotein. Arterioscler. Thromb. Vasc. Biol. 17:3469-74,
1997.
153. V´asquez-Vivar, J., Martasek, P., Hogg, N., Masters, B. S. S., Pritchard, K. A. Jr., and KALYANARAMAN,
B. Endothelial nitric oxide synthase-dependent superoxide generation from adriamycin. Biochemistry 36:11293-7,
1997.
154. Ferguson, E., Parthasarathy, S., Joseph, J., and KALYANARAMAN, B. Generation and initial
characterization of a novel polyclonal antibody directed against homocysteine thiolactone-mediated low-density
lipoprotein. J. Lipid Res. 39:925-33, 1998.
155. Konorev, E. A., Hogg, N., and KALYANARAMAN, B. Rapid and irreversible inhibition of creatine kinase
by peroxynitrite. FEBS Lett. 427:171-4, 1998.
156. Singh, R. J., Karoui, H., Gunther, M. R., Beckman, J. S., Mason, R. P., and KALYANARAMAN, B.
Reexamination of the mechanism of hydroxyl radical adducts formed from the reaction between familial
amyotrophic lateral sclerosis-associated Cu,Zn superoxide dismutase mutants and H2O2. Proc. Natl. Acad. Sci.
USA 95:6675-80, 1998.
157. V´asquez-Vivar, J., KALYANARAMAN, B., Martasek, P., Hogg, N., Masters, B. S. S., Karoui, H., Tordo, P.,
and Pritchard, K. A. Jr. Superoxide generation by endothelial nitric oxide synthase--the influence of cofactors.
Proc. Natl. Acad. Sci. USA 95:9220-5, 1998.
158. Singh, R. J., Goss, S. P. A., Joseph, J., and KALYANARAMAN, B. Nitration of gamma-tocopherol and
oxidation of alpha-tocopherol by copper-zinc superoxide dismutase/H2O2/NO2 : Role of nitrogen dioxide free
radical. Proc. Natl. Acad. Sci. USA 95:12912-7, 1998.
159. Hogg, N. and KALYANARAMAN, B. Nitric oxide and low-density lipoprotein oxidation. Free Radic. Res.
28:593-600, 1998. Review.
160. Baker, J.E., Holman, P., KALYANARAMAN, B., and Pritchard, K.A. Jr. Adaptation of hearts to chronic
hypoxia increases tolerance to subsequent ischemia by increased nitric oxide production. Adv. Exp. Med. Biol.
454:203 17, 1998.
161. Singh, R. J., Hogg, N., Joseph, J., Konorev, E., and KALYANARAMAN, B. The peroxynitrite generator,
SIN-1, becomes a nitric oxide donor in the presence of electron acceptors. Arch. Biochem. Biophys. 361:331-9,
1999.
162. Ferguson, E., Hogg, N., Antholine, W. E., Joseph, J., Singh, R. J., Parthasarathy, S., and
KALYANARAMAN, B. Characterization of the adduct formed from the reaction between homocysteine
thiolactone and low-density lipoprotein: antioxidant implications. Free Radic. Biol. Med. 26:968-77, 1999.
163. Goss, S. P. A., Hogg, N., and KALYANARAMAN, B. The effect of alpha-tocopherol on the nitration of
gamma-tocopherol by peroxynitrite. Arch. Biochem. Biophys. 363, 333-40, 1999.
164. Janssen, Y. M. W., Soultanakis, R., Steece, K., Heerdt, E., Singh, R. J., Joseph, J., and KALYANARAMAN,
B. Depletion of nitric oxide causes cell cycle alterations, apoptosis, and oxidative stress in pulmonary cells. Am. J.
Physiol. 275 (Lung Cell. Mol. Physiol. 19): L1100-9, 1998.
165. Konorev, E. A., Kennedy, M. C., and KALYANARAMAN, B. Cell-permeable superoxide dismutase and
glutathione peroxidase mimetics afford superior protection against doxorubicin-induced cardiotoxicity. Arch.
Biochem. Biophys. 368:421-8, 1999.
166. Singh, R. J., Hogg, N., Goss, S. P. A., Antholine, W. E., and KALYANARAMAN, B. Mechanism of
SOD/H2O2-mediated nitric oxide release from S-nitrosoglutathione--Role of glutamate. Arch. Biochem. Biophys.
372:8-15, 1999.
167. V´asquez-Vivar, J., Hogg, N., Martasek, P., Karoui, H., Pritchard, K. A. Jr., and KALYANARAMAN, B.
Tetrahydrobiopterin-dependent inhibition of superoxide generation from neuronal nitric oxide synthase. J. Biol.
Chem. 274:26736-42, 1999.
168. V´asquez-Vivar, J., Hogg, N., Martasek, P., Karoui, H., Tordo, P., Pritchard, K. A. Jr., and
KALYANARAMAN, B. Effect of redox-active drugs on superoxide generation from nitric oxide synthases:
biological and toxicological implications. Free Radic. Res. 31:607-17, 1999. Review.
169. Goss, S. P. A., Singh, R. J., Hogg, N., and KALYANARAMAN, B. Reactions of •NO, •NO2, and
peroxynitrite in membranes: physiological implications. Free Radic. Res. 31:597-606, 1999. Review.
170. Goss, S. P. A., Singh, R. J., and KALYANARAMAN, B. Bicarbonate enhances the peroxidase activity of
Cu,Zn-superoxide dismutase: role of carbonate anion radical. J. Biol. Chem. 274:28233-9, 1999.
171. Ferguson, E., Hogg, N., Antholine, W. E., Joseph, J., Singh, R. J., Parthasarathy, S., and
KALYANARAMAN, B. Characterization of the adduct formed from the reaction between homocysteine
thiolactone and low-density lipoprotein: antioxidant implications. Free Radic. Biol. Med. 26:968-77, 1999.
172. Singh, R. J., Hogg, N., Joseph, J., Konorev, E., and KALYANARAMAN, B. The peroxynitrite generator,
SIN-1, becomes a nitric oxide donor in the presence of electron acceptors. Arch. Biochem. Biophys. 361:331-9,
1999.
173. Konorev, E. A., Kennedy, M. C., and KALYANARAMAN, B. Cell-permeable superoxide dismutase and
glutathione peroxidase mimetics afford superior protection against doxorubicin-induced cardiotoxicity. Arch.
Biochem. Biophys. 368:421-8, 1999.
174. V´asquez-Vivar, J., Hogg, N., Martasek, P., Karoui, H., Pritchard, K. A. Jr., and KALYANARAMAN, B.
Tetrahydrobiopterin-dependent inhibition of superoxide generation from neuronal nitric oxide synthase. J. Biol.
Chem. 274:26736-42, 1999.
175. Patel, R. P., Hogg, N., Spencer, N. Y., KALYANARAMAN, B., Matalon, S., and Darley-Usmar, V. M.
Biochemical characterization of human S-nitrosohemoglobin. Effects on oxygen binding and transnitrosation. J.
Biol. Chem. 274:15487-92, 1999.
176. Goss, S. P. A., Singh, R. J., and KALYANARAMAN, B. Bicarbonate enhances the peroxidase activity of Cu,
Zn-superoxide dismutase. J. Biol. Chem. 274:28233-329, 1999.
177. Baker, J. E., Holman, P., KALYANARAMAN, B., Griffith, O. W., and Pritchard, K. A. Jr.: Adaptation to
chronic hypoxia confers tolerance to subsequent myocardial ischemia by increased nitric oxide production. Ann.
N.Y. Acad Sci. 874:236-53, 1999.
178. Hogg, N. and KALYANARAMAN, B. Nitric oxide and lipid peroxidation. Biochim. Biophys. Acta
1411:378-84, 1999. Review.
179. Zhang, H., Joseph, J., Felix, C., and KALYANARAMAN, B. Bicarbonate enhances the hydroxylation,
nitration, and peroxidation reactions catalyzed by Cu, Zn-superoxide dismutase: Intermediacy of carbonate anion
radical. J. Biol.Chem. 275:14038-45, 2000.
180. Konorev, E.A., KALYANARAMAN, B., and Hogg, N. Modification of creatine kinase by s-nitrosothiols: snitrosation vs. s-thiolation. Free Radic. Biol. Med. 28:1671-8, 2000.
181. Shi, Y., Pritchard, K. A., Holman, P., Rafiee, P., Griffith, O. W., KALYANARAMAN, B., and Baker, J. E.
Chronic myocardial hypoxia increases nitric oxide synthase and decreases caveolin-3. Free Radic. Biol. Med.
29:695-703, 2000.
182. Konorev, E. A., Zhang, H., Joseph, J., Kennedy, M. C., and KALYANARAMAN, B. Bicarbonate exacerbates
oxidative injury induced by antitumor antibiotic doxorubicin in cardiomyocytes. Am. J. Physiol. Heart Circ.
Physiol. 279:H2424-30, 2000.
183. V´asquez-Vivar, J. and KALYANARAMAN, B. Generation of superoxide from nitric oxide synthase. FEBS
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184. Pritchard, K. A. Jr, Ackerman, A., and KALYANARAMAN, B. Chromium (VI) increases endothelial cell
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185. Kotamraju, S., Konorev, E. A., Joseph, J., and KALYANARAMAN, B. Doxorubicin-induced apoptosis in
endothelial cells and cardiomyocytes is ameliorated by nitrone spin traps and ebselen. Role of reactive oxygen and
nitrogen species. J. Biol. Chem. 275, 33585-92, 2000.
186. Brzezinska, A.K., Gebremedhin, D., Chilian, W. M., KALYANARAMAN, B., and Elliot, S. J. Peroxynitrite
reversibly inhibits CA(2+)-activated K(+) channels in rat cerebral artery smooth muscle cells. Am. J. Physiol.
Heart Circ. Physiol. 278:H1883-90, 2000.
187. Zhang, H., Joseph, J., V´asquez-Vivar, J., Karoui, H., Nsanzumuhire, C., Martasek, P., Tordo, P., and
KALYANARAMAN, B. Detection of superoxide anion using an isotopically labeled nitrone spin trap: Potential
biological implications. FEBS Lett. 473:58-62, 2000.
188. V´asquez-Vivar, J., KALYANARAMAN, B., and Kennedy, M. C. Mitochondrial aconitase as a source of
hydroxyl radical. An electron spin resonance investigation. J. Biol. Chem. 275:14064-9, 2000.
189. Kotamraju, S., Hogg, N., Joseph, J., Keefer, L. K., and KALYANARAMAN, B. Inhibition of oxidized lowdensity lipoprotein-induced apoptosis in endothelial cells by nitric oxide: peroxyl radical scavenging as an
antiapoptotic mechanism. J. Biol. Chem. 276:17316-23, 2001.
190. Zhang, H., Joseph, J., Feix, J., Hogg, N., and KALYANARAMAN, B. Nitration and oxidation of a
hydrophobic tyrosine probe by peroxynitrite in membranes: comparison with nitration and oxidation of tyrosine by
peroxynitrite in aqueous solution. Biochemistry 40:7675-86, 2001.
191. Baker, J. E., Contney, S. J., Singh, R., KALYANARAMAN, B., Gross, G. J., and Bosnjak, Z. J. Nitric oxide
activates the sarcolemmal K(ATP) channel in normoxic and chronically hypoxic hearts by a cyclic GMP-dependent
mechanism. J. Mol. Cell. Cardiol. 33:331-41, 2001.
192. Zhao, H., Joseph, J., Zhang, H., Karoui, H., and KALYANARAMAN, B. Synthesis and biochemical
applications of a solid cyclic nitrone spin trap: a relatively superior trap for detecting superoxide anions and
glutathiyl radicals. Free Radic. Biol. Med. 31, 599-606, 2001.
193. Kalivendi, S. V., Kotamraju, S., Zhao, H., Joseph, J., and KALYANARAMAN, B. Doxorubicin-induced
apoptosis is associated with increased transcription of endothelial nitric oxide synthase: effect of antiapoptotic
antioxidants and calcium. J. Biol. Chem. 276:47266-76, 2001.
194. V´asquez-Vivar, J., Whitsett, J., Mart´asek, P., Hogg, N., and KALYANARAMAN, B. Reaction of
tetrahydrobiopterin with superoxide: EPR-kinetic analysis and characterization of the pteridine radical. Free Radic.
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195. Patel, R. P., Boersma, B.J., Crawford, J. H., Hogg, N., Kirk, M., KALYANARAMAN, B., Parks, D. A.,
Barnes, S., and Darley-Usmar, V. Antioxidant mechanisms of isoflavones in lipid systems: paradoxical effects of
peroxyl radical scavenging. Free Radic. Biol. Med. 31:1570-81, 2001.
196. Schroeder, P., Zhang, H., Klotz, L. O., KALYANARAMAN, B., and Sies, H. (-)-Epicatechin inhibits nitration
and dimerization of tyrosine in hydrophilic as well as hydrophobic environments. Biochem. Biophys. Res.
Commun. 289:1334-8, 2001.
197. KALYANARAMAN, B., Joseph, J., and Zhang, H. Bicarbonate enhances nitration and oxidation reactions in
biological systems--role of reactive oxygen and nitrogen species. Adv. Exp. Med. Biol. 500:175-82, 2001. Review.
198. Zhang, H., Joseph, J., Gurney, M., Becker, D., and KALYANARAMAN, B. Bicarbonate enhances peroxidase
activity of copper, zinc superoxide dismutase - role of carbonate anion radical and scavenging of carbonate anion
radical by metalloporphyrin antioxidant enzyme mimetics. J. Biol. Chem. 277:1013-20, 2002.
199. V´asquez-Vivar, J., Mart´asek, P., Whitsett, J., Joseph, J., and KALYANARAMAN, B. The ratio between
tetrahydrobiopterin and oxidized tetrahydrobiopterin analogues controls superoxide release from endothelial nitric
oxide synthase: an EPR spin trapping study. Biochem. J. 362:733-9, 2002.
200. Zhu, B. Z., Zhao, H. T., KALYANARAMAN, B., and Frei, B. Metal-independent production of hydroxyl
radicals by halogenated quinones and hydrogen peroxide: an ESR spin trapping study. Free Radic. Biol. Med.
32:465-73, 2002.
201. Kotamraju, S., Chitambar, C. R., Kalivendi, S. V., Joseph, J., and KALYANARAMAN, B. Transferrin
receptor-dependent iron uptake is responsible for doxorubicin-mediated apoptosis in endothelial cells. J. Biol.
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202. KALYANARAMAN, B., Joseph, J., Kalivendi, S., Wang, S., Konorev, E., Kotamraju S. Doxorubicininduced apoptosis: implications in cardiotoxicity. Mol. Cell. Biochem. 234-235:119-24, 2002.
203. Konorev, E., Kotamraju, S., Zhao, H., Kalivendi, S., Joseph, J., and KALYANARAMAN, B. Paradoxical
effects of metalloporphyrins on doxorubicin-induced apoptosis: scavenging of reactive oxygen species versus
induction of heme oxygenase-1. Free Radic. Biol. Med. 33:988-97, 2002.
204. V´asquez-Vivar, J., Duquaine, D., Whitsett, J., KALYANARAMAN, B., and Rajagopalan, S. Altered
tetrahydrobiopterin metabolism in atherosclerosis: implications for use of oxidized tetrahydrobiopterin analogues
and thiol antioxidants. Arterioscler. Thromb. Vasc. Biol. 22:1655-61, 2002.
205. Newman, D. K., Hoffman, S., Kotamraju, S., Zhao, T., Wakim, B., KALYANARAMAN, B., Newman, P. J.
Nitration of PECAM-1 ITIM tyrosines abrogates phosphorylation and SHP-2 binding. Biochem. Biophys. Res.
Commun. 296:1171-9, 2002.
206. Wang, S., Kotamraju, S., Konorev, E., Kalivendi, S., Joseph, J., KALYANARAMAN, B. Activation of
nuclear factor-kappaB during doxorubicin-induced apoptosis in endothelial cells and myocytes is pro-apoptotic: the
role of hydrogen peroxide. Biochem. J. 367:729-40, 2002.
207. Tampo, Y., Kotamraju, S., Chitambar, C. R., Kalivendi, S. V., Keszler, A., Joseph, J., KALYANARAMAN,
B. Oxidative stress-induced iron signaling is responsible for peroxide-dependent oxidation of
dichlorodihydrofluorescein in endothelial cells. Circ. Res. 92:56-63, 2003.
208. V´asquez-Vivar, J., KALYANARAMAN, B., Mart´asek, P. The role of tetrahydrobiopterin in superoxide
generation from eNOS: enzymology and physiological implications. Free Radic. Res. 37:121-7, 2003. Review.
209. Zhang, H., Bhargava, K., Keszler, A., Feix, J., Hogg, N., Joseph, J., KALYANARAMAN, B. Transmembrane
nitration of hydrophobic tyrosyl peptides - localization, characterization, mechanism of nitration and biological
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210. Kalivendi, S. V., Kotamraju, S., Cunningham, S., Shang, T., Hillard, C. J., KALYANARAMAN, B. 1Methyl-4-phenylpyridinium (MPP+)-induced apoptosis and mitochondrial oxidant generation: role of transferringreceptor-dependent iron and hydrogen peroxide. Biochem. J. 371:151-64, 2003.
211. Shang, T., Uihlein, A. V., Van Asten, J., KALYANARAMAN, B., Hillard, C. J. 1-Methyl-4phenylpyridinium (MPP+) accumulates in cerebellar granule neurons via organic cation transporter-3 (OCT3). J.
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212. Zhao, H., Kalivendi, S., Zhang, H., Joseph, J., Nithipatikom, K., V´asquez-Vivar, J., KALYANARAMAN, B.
Superoxide reacts with hydroethidine but forms a fluorescent product that is distinctly different from ethidium:
potential implications in intracellular fluorescence detection of superoxide. Free Radic. Biol. Med. 34:1359-68,
2003.
213. Zhang, H., Andrekopoulos, C., Joseph, J., Chandran, K., Karoui, H. Crow, J. P., KALYANARAMAN, B.
Bicarbonate-dependent peroxidase activity of human Cu,Zn-superoxide dismutase induces covalent aggregation of
protein: Intermediacy of tryptophan-derived oxidation products. J. Biol. Chem. 278:24078-89, 2003.
214. Audi, S.H., Zhao, H., Bongard, R.D., Hogg, N., Kettenhofen, N.J., KALYANARAMAN, B., Dawson, C.A.,
Merker, M.P. Pulmonary arterial endothelial cells affect the redox status of coenzyme Q(0). Free Radic. Biol. Med.
34:892-907, 2003.
215. Rassaf, T., Bryan, N.S., Maloney, R.E., Specian, V., Kelm, M., KALYANARAMAN, B., Rodriguez, J.,
Feelisch, M. NO adducts in mammalian red blood cells: too much or too little? Nat. Med. 9:481-2, 2003; author
reply 482-3.
216. Khan, N., Wilmot, C.M., Rosen, G.M., Demidenko, E., Sun, J., Joseph, J., O'Hara, J., KALYANARAMAN,
B., Swartz, H.M. Spin traps: in vitro toxicity and stability of radical adducts. Free Radic. Biol. Med. 34:1473-81,
2003.
217. Ogawa, H., Rafiee, P., Heidemann, J., Fisher, P.J., Johnson, N.A., Otterson, M.F., KALYANARAMAN, B.,
Pritchard, K.A. Jr., Binion, D.G. Mechanisms of endotoxin tolerance in human intestinal microvascular endothelial
cells. J. Immunol. 170:5956-64, 2003.
218. Liu, Y., Zhao, H., Li, H., KALYANARAMAN, B., Nicolosi, A.C., Gutterman, D.D. Mitochondrial sources of
H2O2 generation play a key role in flow-mediated dilation in human coronary resistance arteries. Circ. Res.
93:573-80, 2003.
219. Kotamraju, S., Tampo, Y., Keszler, A., Chitambar, C.R., Joseph, J., Haas, A.L., KALYANARAMAN, B.
Nitric oxide inhibits H2O2-induced transferrin receptor-dependent apoptosis in endothelial cells: Role of ubiquitinproteasome pathway. Proc. Natl. Acad. Sci. USA 100(19):10653-8, 2003.
220. Duquaine, D., Hirsch, G.A., Chakrabarti, A., Han, Z., Kehrer, C., Brook, R., Joseph, J., Schott, A.,
KALYANARAMAN, B., V´asquez-Vivar, J., Rajagopalan, S. Rapid-onset endothelial dysfunction with
adriamycin: evidence for a dysfunctional nitric oxide synthase. Vasc. Med. 8:101-7, 2003.
221. Maas, M., Wang, R., Paddock, C., Kotamraju, S., KALYANARAMAN, B., Newman, P.J., Newman, D.K.
Reactive oxygen species induce reversible PECAM-1 tyrosine phosphorylation and SHP-2 binding. Am. J. Physiol.
Heart Circ. Physiol. 285:H2336-44, 2003.
222. Keszler, A., KALYANARAMAN, B., Hogg, N. Comparative investigation of superoxide trapping by cyclic
nitrone spin traps: the use of singular value decomposition and multiple linear regression analysis. Free Radic.
Biol. Med. 35:1149-57, 2003.
223. Kotamraju, S., Tampo, Y., Kalivendi, S.V., Joseph, J., Chitambar. C.R., KALYANARAMAN, B. Nitric oxide
mitigates peroxide-induced iron signaling, oxidative damage, and apoptosis in endothelial cells: role of proteasomal
function? Arch. Biophys. Biochem. 423:74-80, 2004. Review.
224. Shang, T., Kotamraju, S., Kalivendi, S.V., Hillard, C.J., KALYANARAMAN, B. 1-Methyl-4phenylpyridinium-induced apoptosis in cerebellar granule neurons is mediated by transferrin receptor irondependent depletion of tetrahydrobiopterin and neuronal nitric oxide synthase-derived superoxide. J. Biol. Chem.
279:19099-112, 2004.
225. Andrekopoulos, C., Zhang, H., Joseph, J., Kalivendi, S., KALYANARAMAN, B. Bicarbonate enhances
alpha-synuclein oligomerization and nitration: intermediacy of carbonate radical anion and nitrogen dioxide
radical. Biochem. J. 378:435-47, 2004.
226. Kalivendi, S.V., Cunningham, S. Kotamraju, S., Joseph, J., Hillard, C.J., KALYNARAMAN, B. Alphasynuclein up-regulation and aggregation during MPP+-induced apoptosis in neuroblastoma cells: Intermediacy of
transferrin receptor iron and hydrogen peroxide. J. Biol. Chem. 279:15240-7, 2004.
227. Wang, S., Konorev, E.A., Kotamraju, S., Joseph, J., Kalivendi, S., KALYANARAMAN, B. Doxorubicin
induces apoptosis in normal and tumor cells via distinctly different mechanisms: Intermediacy of H2O2- and p53dependent pathways. J. Biol. Chem. 279:25535-43, 2004.
228. Kotamraju, S., Kalivendi, S.V., Konorev, E., Chitambar, C.R., Joseph, J., KALYANARAMAN, B. Oxidantinduced iron signaling in Doxorubicin-mediated apoptosis. Methods Enzymol. 378:362-82, 2004.
229. KALYANARAMAN, B. Introduction to the review series "Protein Radicals in Biological Systems--ESR Spin
Trapping and Immuno Spin Trapping Approaches." Free Radic. Biol. Med. 36:1071, 2004.
230. Matsunaga, T., Kotamraju, S., Kalivendi, S.V., Dhanasekaran, A., Joseph, J., KALYANARAMAN, B.
Ceramide-induced intracellular oxidant formation, iron signaling, and apoptosis in endothelial cells: protective role
of endogenous nitric oxide. J. Biol. Chem. 279: 28614-24, 2004.
231. Karunakaran, C., Zhang, H., Crow, J.P., Antholine, W.E., KALYANARAMAN, B. Direct probing of copper
active site and free radical formed during bicarbonate-dependent peroxidase activity of bovine and human copper,
zinc superoxide dismutases: Low-temperature electron paramagnetic resonance and electron nuclear double
resonance studies. J. Biol. Chem. 279:32534-40, 2004.
232. Zhang, H., Andrekopoulos, C., Joseph, J., Crow, J., KALYANARAMAN, B. The carbonate radical anioninduced covalent aggregation of human copper,zinc superoxide dismutase and alpha-synuclein - intermediacy of
tryptophan- and tyrosine-derived oxidation products. Free Radic. Biol. Med. 36:1355-65, 2004. Review.
233. Merker, M.P., Bongard, R.D., Krenz, G.S., Zhao, H., Fernandes, V.S., KALYANARAMAN, B., Hogg, N.,
Sudi, S.H. Impact of pulmonary arterial cells on duroquinone redox status. Free Radic. Biol. Med. 37, 86-103,
2004.
234. KALYANARAMAN, B. Nitrated lipids: a class of cell-signaling molecules. Proc. Natl. Acad. Sci. USA
101:11527-8, 2004. Review.
235. Dhanasekaran, A., Kotamraju, S., Kalivendi, S.V., Matsunaga, T., Shang, T., Keszler, A., Joseph, J.,
KALYANARAMAN, B. Supplementation of endothelial cells with mitochondria-targeted antioxidants inhibit
peroxide-induced mitochondrial iron uptake, oxidative damage, and apoptosis. J. Biol. Chem. 279:37575-87, 2004.
236. Zhang, H., Joseph, J., Crow, J., KALYANARAMAN, B. Mass spectral evidence for carbonate-anion-radicalinduced posttranslational modification of tryptophan to kynurenine in human Cu, Zn superoxide dismutase. Free
Radic. Biol. Med. 37:2018-26, 2004.
237. Kalivendi, S., Hatakeyama, K., Whitsett, J., Konorev, E., KALYANARAMAN, B., Vasquez-Vivar, J.
Changes in tetrahydrobiopterin levels in endothelial cells and adult cardiomyocytes induced by LPS and hydrogen
peroxide--A role for GFRP? Free Radic. Biol. Med. 38:481-91, 2005.
238. Trujillo, M., Folkes, L., Bartesaghi, S., KALYANARAMAN, B., Wardman, P., Radi, R. Peroxynitrite-derived
carbonate and nitrogren dioxide radicals readily react with lipoic and dihydrolipoic acid. Free Radic. Biol. Med.
39:279-88, 2005.
239. Zhao, H., Joseph, J., Fales, H.M., Sokoloski, E.A., Levine, R.L., Vasquez-Vivar, J., KALYANARAMAN, B.
Detection and characterization of the product of hydroethidine and intracellular superoxide by HPLC and
limitations of fluorescence. Proc. Natl. Acad. Sci. USA 102:5727-32, 2005. [Erratum: Proc. Natl. Acad. Sci. USA
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240. Dhanasekaran, A., Kotamraju, S., Karunakaran, C., Kalivendi, S.V., Thomas, S., Joseph, J.,
KALYANARAMAN, B. Mitochondria superoxide dismutase mimetic inhibits peroxide-induced oxidative damage
and apoptosis: role of mitochondrial superoxide. Free Radic. Biol. Med. 39:567-83, 2005.
241. Shi, H., Timmins, G., Monske, M., Burdick, A., KALYANARAMAN, B., Liu, Y., Clement, J.L., Burchiel, S.,
Liu, K.J. Evaluation of spin trapping agents and trapping conditions for detection of cell-generated reactive oxygen
species. Arch. Biochem. Biophys. 437:59-68, 2005.
242. Kalivendi, S.V., Konorev, E.A., Cunningham, S., Vanamala, S.K., Kaji, E.H., Joseph, J.,
KALYANARAMAN, B. Doxorubicin activates nuclear factor of activated T-lymphocytes and Fas ligand
transcription: role of mitochondrial reactive oxygen species and calcium. Biochem. J. 389:527-39, 2005.
243. Karunakaran, C., Zhang, H., Joseph, J., Antholine, W.E., KALYANARAMAN, B. Thiol oxidase activity of
copper, zinc superoxide dismutase stimulates bicarbonate-dependent peroxidase activity via formation of a
carbonate radical. Chem. Res. Toxicol. 18:494-500, 2005.
244. Zielonka, J., Zhao, H., Xu, Y., KALYANARAMAN, B. Mechanistic similarities between oxidation at
hydroethidine by Fremy's salt and superoxide: stopped-flow optical and EPR studies. Free Radic. Biol. Med.
39:853-63, 2005.
245. Shang, T., Joseph, J., Hillard, C.J., KALYANARAMAN, B. Death-associated protein kinase as a sensor of
mitochondrial membrane potential - Role of lysosome in mitochondrial toxin induced cell death. J. Biol. Chem.
280, 34644-53, 2005.
246. Sun, G., Zhao, H., KALYANARAMAN, B., Dahms, N.M. Identification of residues essential for
carbohydrate recognition and cation dependence of the 46 kDa mannose 6-phosphate receptor. Glycobiology
15:1136-49, 2005.
247. Porter, R., Jachymova, M., Martasek, P., KALYANARAMAN, B., Vasquez-Vivar, J. Reductive activation of
Cr(Vi) by nitric oxide synthase. Chem. Res. Toxicol. 18:834-43, 2005.
248. Chi, L., Ke, Y., Luo, C., Li, B., Gozal, D., KALYANARAMAN, B., Liu, R. Motor neuron degeneration
promotes neural progenitor cell proliferation, migration, and neurogenesis in the spinal cords of ALS mice. Stem
Cells 24:34-43, 2006.
249. Zhang, H., Xu, Y., Joseph, J., KALYANARAMAN, B. Intramolecular electron transfer between tyrosyl
radical and cysteine residue inhibits tyrosine nitration and induces thiyl radical formation in model peptides treated
with myeloperoxidase, H2O2, and NO2-: EPR spin-trapping studies. J. Biol. Chem. 280:40684-98, 2005.
250. Kotamraju, S., Matalon, S., Matsunaga, T., Shang, T., Hickman-Davis, J.M., KALYANARAMAN, B.
Upregulation of immunoproteasomes by nitric oxide: potential antioxidative mechanism in endothelial cells. Free
Radic. Biol. Med. 40:1034-44, 2006.
251. Shang, T., Kotamraju, S., Zhao, H., Kalivendi, S.V., Hillard, C.J., KALYANARAMAN, B. Sepiapterin
attenuates 1-methyl-4-phenylpyridinium-induced apoptosis in neuroblastoma cells transfected with neuronal NOS:
Role of tetrahydrobiopterin, nitric oxide, and proteasome activation. Free Radic. Biol. Med. 39:1059-74, 2005.
252. Bartesaghi, S., Valez, V., Trujillo, M., Peluffo, G., Romero, N., Zhang, H., KALYANARAMAN, B., Radi, R.
Mechanistic studies of peroxynitrite-mediated tyrosine nitration in membranes using the hydrophobic probe N-tBOC L-tyrosine tert butyl ester (BTBE). Biochemistry 45:6813-25, 2006.
253. Huh, J., Liepins, A., Zielonka, J., Andrekopoulos, C., KALYANARAMAN, B., Sorokin, A. Cyclooxygenase
2 rescues LNCaP prostate cancer cells from sanguinarine-induced apoptosis by a mechanism involving inhibition
of nitric oxide synthase activity. Cancer Res. 66:3726-36, 2006.
254. Zielonka, J., Vasquez-Vivar, J., KALYANARAMAN, B. The confounding effects of light, sonication and
Mn(III)TBAP on quantitation of superoxide using hydroethidine. Free Radic. Biol. Med. 41:1050-7, 2006.
255. Whitsett, J., Martásek, P., Zhao H, Schauer DW, Hatakeyama K, KALYANARAMAN, B., Vásquez-Vivar J.
Endothelial cell superoxide anion radical generation is not dependent on endothelial nitric oxide synthase-serine
1179 phosphorylation and endothelial nitric oxide synthase dimer/monomer distribution. Free Radic. Biol. Med.
40:2056-68, 2006.
256. Zielonka, J., Sarna, T., Roberts, J.E., Wishart, J.F., KALYANARAMAN, B. Pulse radiolysis and steady-state
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257. Watanabe, N., Zmijewski, J.W., Takabe, W., Umezu-Goto, M., LeGoffe, C., Sekine, A., Landar, A.,
Watanabe, A., Aoki, J., Arai, H., Kodama, T., Murphy, M.P., KALYANARAMAN, B., Darley-Usmar, V.M.,
Noguchi, N. Activation of mitogen-activated protein kinases by lysophosphatidylcholine-induced mitochondrial
reactive oxygen species generation in endothelial cells. Am. J. Pathol. 168:1737-48, 2006.
258. Borthiry, G.R., Antholine, W.E., KALYANARAMAN, B., Myers, J.M., Myers, C.R. Reduction of hexavalent
chromium by human cytochrome b(5): Generation of hydroxyl radical and superoxide. Free Radic. Biol. Med.
42:738-55, discussion 735-7, 2007. [PMCID: PMC1993546]
259. Xu, Y., KALYANARAMAN, B. Synthesis and ESR studies of a novel cyclic nitrone spin trap attached to a
phosphonium group--a suitable trap for mitochondria-generated ROS? Free Radic. Res. 41:1-7, 2007.
260. Thomas, S., Kotamraju, S., Zielonka, J., Harder, D.R., KALYANARAMAN, B. Hydrogen peroxide induces
nitric oxide and proteosome activity in endothelial cells: a bell-shaped signaling response. Free Radic. Biol. Med.
42:1049-61, 2007. [PMCID: PMC2692187]
261. Horowitz, S., Binion, D.G., Nelson, V.M., Kanaa, Y., Javadi. P., Lazarova, Z., Andrekopoulos, C.,
KALYANARAMAN, B., Otterson, M.F., Rafiee, P. Increased arginase activity and endothelial dysfunction in
human inflammatory bowel disease. Am. J. Physiol. Gastrointest. Liver Physiol. 292:G1323-36, 2007.
262. Bartesaghi, S., Ferrer-Sueta, G., Peluffo, G., Valez, V., Zhang, H., KALYANARAMAN, B., Radi, R. Protein
tyrosine nitration in hydrophilic and hydrophobic environments. Amino Acids 32:501-15, 2007. Review.
263. Hardy, M., Chalier, F., Ouari, O., Finet, J.P., Rockenbauer, A., KALYANARAMAN, B., Tordo, P. MitoDEPMPO synthesized from a novel NH2-reactive DEPMPO spin trap: a new and improved trap for the detection
of superoxide. Chem. Commun. (Camb.) (10):1083-5, 2007
264. Zhu, B.Z., Zhao, H.T., KALYANARAMAN, B., Liu, J., Shan, G.Q., Du, Y.G., Frei, B. Mechanism of metalindependent decomposition of organic hydroperoxides and formation of alkoxyl radicals by halogenated quinones.
Proc. Natl. Acad. Sci. USA 104(10):3698-702, 2007.
265. Hardy, M., Rockenbauer, A., Vasquez-Vivar, J., Felix, C., Lopez, M., Srinivasan, S., Avadhani, N., Tordo, P.,
KALYANARAMAN, B. Detection, characterization, and decay kinetics of ROS and thiyl adducts of mitoDEPMPO spin trap. Chem. Res. Toxicol. 20:1053-60, 2007. [NIHMSID: 515877]
266. Kotamraju, S., Williams, C.L., KALYANARAMAN, B. Statin-induced breast cancer cell death: Role of
inducible nitric oxide and arginase-dependent pathways. Cancer Res. 67:7386-94, 2007.
267. Migrino, R.Q., Aggarwal, D., Konorev, E., Brahmbhatt, T., Bright, M., KALYANARAMAN, B. Early
detection of doxorubicin cardiomyopathy using two-dimensional strain echocardiography. Ultrasound Med. Biol.
34:208-14, 2008. [PMCID: PMC2582214]
268. Romero, N., Peluffo, G., Bartesaghi, S., Zhang, H., Joseph, J., KALYANARAMAN, B., Radi, R.
Incorporation of the hydrophobic probe N-t-BOC-L-tyrosine tert-butyl ester to red blood cell membranes to study
peroxynitrite-dependent reactions. Chem. Res. Toxicol. 20:1638-48, 2007.
269. Zhu B.-Z., KALYANARAMAN, B., Jiang, G.-B. Molecular mechanism for metal-independent production of
hydroxyl radicals by hydrogen peroxide and halogenated quinones. Proc. Natl. Acad. Sci. USA 104:17575-8, 2007.
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270. Zielonka, J., Srinivasan, S., Hardy, M., Ouari, O., Lopez, M., Vasquez-Vivar, J., Avadhani, N.G.,
KALYANARAMAN, B. Cytochrome c-mediated oxidation of hydroethidine and mito-hydroethidine in
mitochondria: Identification of homo- and heterodimers. Free Radic. Biol. Med. 44:835-46, 2008. [PMCID:
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271. Zielonka, J., Vasquez-Vivar, J., KALYANARAMAN, B. Detection of 2-hydroxyethidium in cellular systems:
a unique marker product of superoxide and hydroethidine. Nat. Protoc. 3:8-21, 2008. PMID: 18193017
272. Matsunaga, T., Kamiya, T., Sumi, D., Kumagai, Y., KALYANARAMAN, B. L-Xylulose reductase is
involved in 9,10-phenanthrenequinone-induced apoptosis in human T lymphoma cells. Free Radic. Biol. Med.
44:1191-202, 2008. PMID: 18206670
273. Vasquez-Vivar, J., Whitsett, J., Ionova, I., Konorev, E., Zielonka, J., KALYANARAMAN, B., Shi, Y., Pieper,
G.M. Cytokines and Linduce inducible nitric oxide synthase but not enzyme activity in adult rat cardiomyocytes.
Free Radic. Biol. Med. 45:994-1001, 2008. [PMCID: PMC2578873]
274. Konorev, E.A., Vanamala, S., KALYANARAMAN, B. Differences in doxorubicin-induced apoptotic
signaling in adult and immature cardiomyocytes. Free Radic. Biol. Med. 45:1723-8, 2008. [PMCID: PMC3039518]
275. Bartesaghi, S., Peluffo, G., Zhang, H., Joseph, J., KALYANARAMAN, B., Radi, R. Tyrosine nitration,
dimerization, and hydroxylation by peroxynitrite in membranes as studied by the hydrophobic probe N-t-BOC-ltyrosine tert-butyl-ester. Methods Enzymol. 441:217-36, 2008. PMID: 18554537
276. Chandran, K., Aggarwal, D., Migrino, R.Q., Joseph, J., McAllister, D., Konorev, E.A., Antholine, W.E.,
Zielonka, J., Srinivasan, S., Avadhani, N.G., KALYANARAMAN, B. Doxorubicin inactivates myocardial
cytochrome c oxidase in rats: Cardioprotection by Mito-Q. Biophys. J. 96:1388-98, 2009. [PMCID: PMC2717244]
277. Zielonka, J., Hardy, M., KALYANARAMAN, B. HPLC study of oxidation products of hydroethidine in
chemical and biological systems: Ramifications in superoxide measurements. Free Radic. Biol. Med. 46:329-38,
2009. [PMCID: PMC3375818]
278. Zhang, H., Zielonka, J., Sikora, A., Joseph, J., Xu, Y., KALYANARAMAN, B. The effect of neighboring
methionine residue on tyrosine nitration and oxidation in peptides treated with MPO, H2O2, and NO2- or
peroxynitrite and bicarbonate: Role of intramolecular electron transfer mechanism? Arch. Biochem. Biophys.
484:134-45, 2009. [PMCID: PMC2874256]
279. Galati, D., Srinivasan, S., Raza, H., Prabu, S.K., Hardy, M., Chandran, K., Lopez, M., KALYANARAMAN,
B., Avadhani, N.G. Role of nuclear encoded subunit Vb in the assembly and stability of cytochrome c oxidase
complex: Implications in mitochondrial dysfunction and ROS production. Biochem. J. 420:439-49, 2009. [PMCID:
PMC2735414]
280. Zhang, H., Andrekopoulos, C., Xu, Y., Joseph, J., Hogg, H., Feix, J., KALYANARAMAN, B. Decreased Snitrosation of peptide thiols in the membrane interior. Free Radic. Biol. Med. 47:962-8, 2009. [PMCID:
PMC2874255]
281. Zhu, B.Z., Shan, G.Q., Huang, C.H., KALYANARAMAN, B., Mao, L., Du, Y.G. Metal-independent
decomposition of hydroperoxides by halogenated quinones: Detection and identification of a quinone ketoxy
radical. Proc. Natl. Acad. Sci. USA 106:11466-71, 2009. [PMCID: PMC2710671]
282. Larson, M., Li, Z., Hall, C.L., Jensen, E., McAllister, D.M., KALYANARAMAN, B., Zhao, M. Physiological
fluctuation of 99mTc-Sestamibi uptake in normal mammary glands: A systematic investigation in female rats. Acta
Radiol. 50:975-8, 2009. PMID: 19636990
283. Sikora, A., Zielonka, J., Lopez, M., Joseph, J., KALYANARAMAN, B. Direct oxidation of boronates by
peroxynitrite: Mechanism and implications in fluorescence imaging of peroxynitrite. Free Radic. Biol. Med.
47:1401-7, 2009. [PMCID: PMC3375817]
284. Weinberg, F., Hamanaka, R., Wheaton, W.W., Weinberg, S., Joseph, J., Lopez, M., KALYANARAMAN, B.,
Mutlu, G.M., Budinger, G.R., Chandel, N.S. Mitochondrial metabolism and ROS generation are essential for Krasmediated tumorigenicity. Proc. Natl. Acad. Sci. USA 107:8788-93, 2010. [PMCID: PMC2889315]
285. Subramanian, S., KALYANARAMAN, B., Migrino, R.Q. Mitochondrially targeted antioxidants for the
treatment of cardiovascular diseases. Recent Pat. Cardiovasc. Drug Discov. 5:54-65, 2010. Review. PMID:
19807685
286. Kalivendi, S.V., Yedlapudi, D., Hillard, C.J., KALYANARAMAN, B. Oxidants induce alternative splicing of
alpha-synuclein: implications for Parkinson's disease. Free Radic. Biol. Med. 48:377-83, 2010. PMID: 19857570
NIHMS ID 640838
287. Li, Z., Lopez, M., Hardy, M., McAllister, D.M., KALYANARAMAN, B., Zhao, M. A 99mTc-labeled
triphenylphosphonium derivative for the early detection of breast tumors. Cancer Biother. Radiopharm. 24:579-87,
2009. [PMCID: PMC2883499]
288. Diers, A.R., Higdon, A.N., Ricart, K.C., Johnson, M.S., Agarwal, A., KALYANARAMAN, B., Landar, A.,
Darley-Usmar, V.M. Mitochondrial targeting of the electrophilic lipid 15-deoxy-Delta12,14-Prostaglandin J2
increases apoptotic efficacy via redox cell signaling mechanisms. Biochem. J. 426:31-41, 2010. [PMCID:
PMC3079364]
289. Zielonka, J., KALYANARAMAN, B. Hydroethidine- and Mito-SOX-derived red fluorescence is not a
reliable indicator of intracellular superoxide formation: Another inconvenient truth. Free Radic. Biol. Med. 48:9831001, 2010. Review. [PMCID: PMC3587154]
290. Zielonka, J., Hardy, M., KALYANARAMAN, B. Corrigendum to "HPLC study of oxidation products of
hydroethidine in chemical and biological systems: Ramifications in superoxide measurements". Free Radic. Biol.
Med. 48:373, 2010. [PMCID: PMC3375818]
291. Bartesaghi, S., Wenzel, J., Trujillo, M., Lopez, M., Joseph, J., KALYANARAMAN, B., Radi, R. Lipid
peroxyl radicals mediate tyrosine dimerization and nitration in membranes. Chem. Res. Toxicol. 23:821-35, 2010.
[PMCID: PMC3506709]
292. Zielonka, J., Sikora, A., Joseph, J., KALYANARAMAN, B. Peroxynitrite is the major species formed from
different flux ratios of co-generated nitric oxide and superoxide: Direct reaction with boronate-based fluorescent
probe. J. Biol. Chem. 285:14210-6, 2010. Featured "Paper of the Week." [PMCID: PMC2863194]
293. Srinivasan, S., Koenigstein, A., Joseph, J., Sun, L., KALYANARAMAN, B., Zaidi, M., Avadhani, N.G. Role
of mitochondrial ROS in osteoclast differentiation. Ann. N. Y. Acad. Sci. 1192:245-52, 2010. [PMCID:
PMC2856121]
294. Dasgupta, J., Kar, S., Liu, R., Joseph, J., KALYANARAMAN, B., Remington, S.J., Chen, C., Melendez, J.A.
Reactive oxygen species control senescence-associated matrix metaolloproteinase-1 through c-Jun-N-terminal
kinase. J. Cell. Physiol. 225:52-62, 2010. [PMCID: PMC2913426]
295. Cheng, Q., Antholine, W.E., Myers, J.M., KALYANARAMAN, B., Arner, E.S., Myers, C.R. The seleniumindependent inherent pro-oxidant NADPH oxidase activity of mammalian thioredoxin reductase and its seleniumdependent direct peroxidase activities. J. Biol. Chem. 285:21708-23, 2010. [PMCID: PMC2898413]
296. Khan, S.A., Nanduri, J., Yuan, G., Kinsman, B., Kumar, G.K., Joseph, J., KALYANARAMAN, B.,
Prabhakar, N.R. NADPH oxidase2 mediates intermittent hypoxia-induced mitochondrial complex I inhibition:
Relevance to blood pressure changes in rats. Antioxid. Redox Signal. 14:533-42, 2011. [PMCID: PMC3038125]
297. Ghosh, A., Chandran, K., Kalivendi, S.V., Joseph, J., Antholine, W.E., Hillard, C.J., Kanthasamy, A.,
Kanthasamy, A., KALYANARAMAN, B. Neuroprotection by mitochondria-targeted drug in Parkinson's disease
model. Free Radic. Biol. Med. 49:1674-84, 2010. [PMCID: PMC4020411]
298. Rao, A., Klein, S.R., Bonar, S.J., Zielonka, J., Mizuno, N., Dickey, J.S., Keller P.W., Joseph, J.,
KALYANARAMAN, B., Shacter, E. The antioxidant transcription factor Nrf2 negatively regulates autophagy and
growth arrest induced by the anticancer redox agent mitoquinine. J. Biol. Chem. 285:34447-59, 2010. [PMCID:
PMC2966059]
299. Chacko, B., Reily, C., Srivastava, A., Johnson, M.S., Ulasova, E., Agarwal, A., Zinn, K., Murphy, M.P.,
KALYANARAMAN, B., Darley-Usmar, V. Prevention of diabetic nephropathy in Ins2+/-AkitaJ mice by the
mitochondria-targeted therapy Mito-Q. Biochem. J. 432:9-19, 2010. [PMCID: PMC2973231]
300. Chandran, K., McCracken, J, Peterson, F.C., Antholine, W.E., Volkman, B.F., KALYANARAMAN, B.
Oxidation of histidine residues in copper-zinc superoxide dismutase by bicarbonate stimulated peroxidase and thiol
oxidase activities: Pulse EPR and NMR studies. Biochemistry 49:10616-22, 2010. [PMCID: PMC3501376]
301. Zhu, B.Z., Mao, L., Fan, R.M., Zhu, J.G., Zhang, Y.N., Wang, J., KALYANARAMAN, B., Frei, B.
Ergothioneine prevents copper-induced oxidative damage to DNA and protein by forming a redox-inactive
ergothioneine-copper complex. Chem. Res. Toxicol. 24:30-4, 2011. PMID: 21047085
302. Shchepin, R., Möller, M.N., Kim, H.Y., Hatch, D.M., Bartesaghi, S., KALYANARAMAN, B., Radi, R.,
Porter, N.A. Tryosine-lipid peroxidation adducts from radical termination: para coupling and intramolecular DielsAlder cyclization. J. Am. Chem. Soc. 132:17490-500, 2010. [PMCID: PMC3677824]
303. Zhu, B.Z., Zhu, J.G., Mao, L., KALYANARAMAN, B., Shan, G.Q. Detoxifying carcinogenic
polyhalogenated quinones by hydroxamic acids via an unusual double Lossen rearrangement mechanism. Proc.
Natl. Acad. Sci. U.S.A. 107:20686-90, 2010. [PMCID: PMC2996439]
304. Kumar, S.N., Konorev, E.A., Aggarwal, D., KALYANARAMAN, B. Analysis of proteome changes in
doxorubicin-treated adult rat cardiomyocyte. J. Proteomics 74:683-97, 2011. [PMCID: PMC3298037]
305. Sikora, A., Zielonka, J., Lopez, M., Dybala-Defratyka, A., Joseph, J., Marcinek, A., KALYANARAMAN, B.
Reaction between peroxynitrite and boronates: EPR spin-trapping, HPLC analyses, and quantum mechanical study
of the free radical pathway. Chem. Res. Toxicol. 24:687-97, 2011. Featured "In this Issue". [PMCID:
PMC3743543]
306. Murphy, M.P., Holmgren, A., Larsson, N.G., Halliwell, B., Chang, C.J., KALYANARAMAN, B., Rhee, S.G.,
Thornally, P.J., Partridge, L, Gems, D., Nyström, T., Belousov, V., Schumacker, P.T., Winterbourn, C.C.
Unraveling the biological roles of reactive oxygen species. Cell Metab. 13:361-6, 2011. PMID: 21459321
307. Cheng, G., Lopez, M., Zielonka, J., Hauser, A.D., Joseph, J., McAllister, D., Rowe, J.J., Sugg, S.L., Williams,
C.L., KALYANARAMAN, B. Mitochondria-targeted nitroxides exacerbate Fluvastatin-mediated cytostatic and
cytotoxic effects in breast cancer cells. Cancer Biol. Ther. 12:707-17, 2011. [PMCID: PMC3218525]
308. Chacko, B.K., Srivastava, A., Johnson, M., Benavides, G.A., Chang, M.J., Ye, Y., Jhala, N., Murphy, M.P.,
KALYANARAMAN, B., Usmar, V.D. The mitochondria-targeted ubiquinone MitoQ decreases ethanol-dependent
micro and macro hepatosteoatosis. Hepatology 54:153-63, 2011. [PMCID: PMC3125473]
309. Trumbull, K.A., McAllister, D., Gandelman, M.M., Fung, W.Y., Lew, T., Brennan, L., Lopez, N. Morré J.,
KALYANARAMAN, B., Beckman, J.S. Diapocynin and apocynin administration fails to significantly extend
survival in G93A SOD1 ALS mice. Neurobiol. Dis. 45:137-44, 2012. [PMCID: PMC3710723]
310. KALYANARAMAN, B. Oxidative chemistry of fluorescent dyes: Implications in the detection of reactive
oxygen and nitrogen species. Biochem. Soc. Trans. 39:1221-5, 2011. PMID: 21936793 Review.
311. Tormos, K.V., Anso, E., Hamanaka, R.B., Eisenhart, J., Joseph, J., KALYANARAMAN, B., Chandel, N.S.
Mitochondrial complex III ROS regulate adipocyte differentiation. Cell Metab. 14:537-44, 2011. [PMCID:
PMC3190168]
312. KALYANARAMAN, B., Darley-Usmar, V., Davies, K.J., Dennery, P.A., Forman, H.J., Grisham, M.B.,
Mann, G.E., Moore, K., Roberts, L.J. II, Ischiropoulos, H. Measuring reactive oxygen and nitrogen species with
fluorescent probes: challenges and limitations. Free Radic. Biol. Med. 52:1-6, 2012. [PMCID: PMC3911769]
313. Mukhopadhyay, P., Horvath, B., Zsengeller, Z., Zielonka, J., Tanchian, G., Holovac, E., Kechrid, M., Patel,
V., Stillman, I.E., Parikh, S.M., Joseph, J., KALYANARAMAN, B., Pacher, P. Mitochondrial-targeted
antioxidants represent a promising approach for prevention of cisplatin-induced nephropathy. Free Radic. Biol.
Med. 52:497-506, 2012. [PMCID: PMC3253235]
314. Zielonka, J., Zielonka, M., Sikora, A., Adamus, J., Hardy, M., Ouari, O., Dranka, B.P., KALYANARAMAN,
B. Global profiling of reactive oxygen and nitrogen species in biological systems: High-throughput real-time
analyses. J. Biol. Chem. 287:2984-95, 2011. Featured "Paper of the Week". [PMCID: PMC3270955]
315. Soberanes, S., Gonzalez, A., Urich, D., Chiarella, S.E., Radigan, K.A., Osornio-Vargas, A., Joseph, J.,
KALYANARAMAN, B., Ridge, K.M., Chandel, N.S., Mutlu, G.M., De Vizcaya-Ruiz, A., Budinger, G.R.S.
Particulate matter air pollution induces hypermethylation of the p16 promoter via a mitochondrial ROS-JNKDNMT1 pathway. Sci. Rep. 2:275, 2012. [PMCID: PMC3281276]
316. Bansal, S., Srinivasan, S., Anandasadagopan, S., Chowdhury, A.R., Selvaraj, .V, KALYANARAMAN, B.,
Joseph, J., Avadhani, N.G. Additive effects of mitochondria-targeted Cytochrome P4502E1 and alcohol toxicity on
cytochrome c oxidase function and the stability of respirosome complexes. J. Biol. Chem. 287:15284-97, 2012.
[PMCID: PMC3346148]
317. Cheng, G., Zielonka, J., Dranka, B.P., McAllister, D., Mackinnon, A.C. Jr., Joseph, J., KALYANARAMAN,
B. Mitochondria targeted drugs synergize with 2-deoxyglucose to trigger breast cancer cell death. Cancer Res.
72:2634-44, 2012. [PMCID: PMC3700358]
318. Zsengellér, Z.K., Ellezian, L., Brown, D., Horváth, B., Mukhopadhyay, P., KALYANARAMAN, B., Parikh,
S.M., Karumanchi, S.A., Stillman, I.E., Pacher, P. Cisplatin nephrotoxicity involves mitochondrial injury with
impaired tubular mitochondrial enzyme activity. J. Histochem. Cytochem. 60:521-9, 2012. [PMCID:
PMC3460350]
319. Petruk, A.A., Bartesaghi, S., Trujillo, M., Estrin, D.A., Murgida, D., KALYANARAMAN, B., Marti, M.A.,
Radi, R. Molecular basis of intramolecular electron transfer in proteins during radical-mediated oxidations:
Computer simulation studies in model tyrosine-cysteine peptides in solution. Arch. Biochem. Biophys. 525:82-91,
2012. [PMCID: PMC3414218]
320. Mukhopadhyay, P., Horváth, B., Zsengellĭ-r, Z., Bátkai, S., Cao, Z., Kechrid, M., Holovac, E., Erdĭ-lyi, K.,
Tanchian, G., Liaudet, L., Stillman, I.E., Joseph, J., KALYANARAMAN, B., Pacher, P. Mitochondrial reactive
oxygen species generation triggers inflammatory response and tissue injury associated with hepatic ischemiareperfusion: therapeutic potential of mitochondrially-targeted antioxidants. Free Radic. Biol. Med. 53:1123-38,
2012. [PMCID: PMC3432152]
321. Zielonka, J., Sikora, A., Hardy, M., Joseph, J., Dranka, B.P., KALYANARAMAN, B. Boronate probes as
diagnostic tools for real time monitoring of peroxynitrite and hydroperoxides. Chem. Res. Toxicol. 25:1793-9,
2012. [PMCID: PMC3501381]
322. Dranka, B.P., Zielonka, J., Kanthasamy, A.G., KALYANARAMAN, B. Alterations in bioenergetics function
induced by Parkinson's disease mimetic compounds: Lack of correlation with superoxide generation. J.
Neurochem. 122:941-51, 2012. [PMCID: PMC3423581]
323. Zhu, B.Z., Mao, L., Huang, C.H., Qin, H., Fan, R.M., KALYANARAMAN, B., Zhu, J.G. Unprecedented
hydroxyl radical-dependent two-step chemiluminescence production by polyhalogenated quinoid carcinogens and
H2O2. Proc. Natl. Acad. Sci. USA 109:16046-51, 2012. [PMCID: PMC3479549]
324. Cunniff, B., Benson, K., Stumpff, J., Newick, K., Held, P., Taatjes, D., Joseph, J., KALYANARAMAN, B.,
Heintz, N.H. Mitochondrial-targeted nitroxides disrupt mitochondrial architecture and inhibit expression of
peroxiredoxin 3 and FOXM1 in malignant mesothelioma cells. J. Cell. Physiol. 228:835-45, 2013. [PMCID:
PMC3928986]
325. Michalski, R., Zielonka, J., Hardy, M., Joseph, J., KALYANARAMAN, B. Hydropropidine: A novel, cellimpermeant fluorogenic probe for detecting extracellular superoxide. Free Radic. Biol. Med. 54:135-47, 2013.
[PMCID: PMC3711142]
326. Ghosh, A., Kanthasamy, A., Joseph, J., Anantharam, V., Srivastava, P., Dranka, B.P., KALYANARAMAN,
B., Kanthasamy, A.G. Anti-inflammatory and neuroprotective effects of an orally active apocynin derivative in preclinical models of Parkinson's disease. J. Neuroinflammation 9(1):241-, 2012. [PMCID: PMC3488558]
327. Valez, V., Cassina, A., Batinic-Haberle, I., KALYANARAMAN, B., Ferrer-Sueta, G., Radi, R. Peroxynitrite
formation in nitric oxide-exposed submitochondrial particles: Detection, oxidative damage and catalytic removal
by Mn-porphyrins. Arch. Biochem. Biophys. 529:45-54, 2013. [PMCID: PMC3534903]
328. Shao, J., Huang, C.H., KALYANARAMAN, B., Zhu, B.Z. Potent methyl oxidation of 5-methyl-2'deoxycytidine by halogenated quinoid carcinogens and hydrogen peroxide via a metal-independent mechanism.
Free Radic. Biol. Med. 60:177-82, 2013. [PMCID: PMC4476646]
329. Myers, J.M., Cheng, Q., Antholine, W., KALYANARAMAN, B., Filipofska, A., Arner, E., Myers, C.R.
Redox activation of Fe(III)-thiosemicarbazones and Fe(III)-bleomycin by thioredoxin reductase: specificity of
enzymatic redox centers and analysis of reactive species formation by ESR spin trapping. Free Radic. Biol. Med.
60:183-94, 2013. [PMCID: PMC3654041]
330. Sikora, A., Zielonka, J., Adamus, J., Debski, D., Dybala-Defratyka, A., Michalowski, B., Joseph, J., Hartley,
R.C., Murphy, M.P., KALYANARAMAN, B. Reaction between peroxynitrite and triphenylphosphoniumsubstituted arylboronic acid isomers: identification of diagnostic marker products and biological implications.
Chem. Res. Toxicol. 26:856-67, 2013. [PMCID: PMC3700382]
331. KALYANARAMAN, B., Dranka, B.P., Hardy, M., Michalski, R., Zielonka, J. HPLC-based monitoring of
products formed from hydroethidine-based fluorogenic probes - The ultimate approach for intra-and extracellular
superoxide detection. Biochim. Biophys. Acta 1840:739-44, 2014. [PMCID: PMC3858408]
332. KALYANARAMAN, B. Teaching the basics of redox biology to medical and graduate students: Oxidants,
antioxidants and disease mechanisms. Redox Biol. 1:244-57, 2013. [PMCID: PMC3757692]
333. Dranka, B.P., Gifford, A., Ghosh, A., Zielonka, J., Joseph, J., Kanthasamy, A.G., KALYANARAMAN, B.
Diapocynin prevents early Parkinson's disease symptoms in the Leucine-Rich Repeat Kinase 2 (LRRK2R1441G)
transgenic mouse. Neurosci. Lett. 549:57-62, 2013. [PMCID: PMC3729885]
334. Qin, H., Huang, C.H., Mao, L., Xia, H.Y., KALYANARAMAN, B., Shao, J., Shan, G.Q., Zhu, B.Z.
Molecular mechanism of metal-independent decomposition of lipid hydroperoxide 13-HPODE by halogenated
quinoid carcinogens. Free Radic. Biol. Med. 63:459-66, 2013. PMID: 23680403
335. Ntantie, E., Gonyo, P., Lorimer, E.L., Hauser, A.D., Schuld, N., McAllister, D., KALYANARAMAN, B.,
Dwinell, M.B., Auchampach, J.A., Williams, C.L. An Adenosine-mediated signaling pathway suppresses
prenylation of the GTPase Rap1B and promotes cell scattering. Sci. Signal. 2013 May 28;6(277):ra39. doi:
10.1126/scisignal.2003374. [PMCID: PMC4278376]
336. Cheng, G., Zielonka, J., McAllister, D.M., Mackinnon, A.C. Jr, Joseph, J., Dwinell, M.B.,
KALYANARAMAN, B. Mitochondria-targeted vitamin E analogs inhibit breast cancer cell energy metabolism
and promotes cell death. BMC Cancer 2013 Jun 13;13(1):285. doi: 10.1186/1471-2407-13-285. [PMCID:
PMC3686663]
337. Dickey, J.S., Gonzalez, Y., Aryal, B., Mog, S., Nakamura, A.J., Redon, C.E., Baxa, U., Rosen, E., Cheng, G.,
Zielonka, J., Parekh, P., Mason, K.P., Joseph, J., KALYANARAMAN, B., Bonner, W., Herman, E., Shacter, E.,
Rao, V.A. Mito-Tempol and dexrazoxane exhibit cardioprotective and chemotherapeutic effects through specific
protein oxidation and autophagy in a syngeneic breast tumor preclinical model. PLoS ONE 8(8):e70575, 2013.
[PMCID: PMC3734284]
338. Dilip, A., Cheng, G., Joseph, J., Kunnimalaiyaan, S., KALYANARAMAN, B., Kunnimalaiyaan, M.,
Gamblin, T.C. Mitochondria-targeted antioxidant and glycolysis inhibition - Synergistic therapy in hepatocellular
carcinoma. Anti-Cancer Drugs 24:881-8, 2013. [PMCID: PMC4028966]
339. Huang, C.H., Shan, G.Q., Mao, L., KALYANARAMAN, B., Qin, H., Ren, F.R., Zhu, B.Z. The first
purification and unequivocal characterization of the radical form of the carbon-centered quinone ketoxy radical
adduct. Chem. Commun. (Camb.) 49:6436-8, 2013. PMID: 23752136
340. Shah, G., Zhang, G., Chen, F., Cao, Y., KALYANARAMAN, B., See, W. Loss of BCG viability adversely
affects the direct response of urothelial carcinoma cells to BCG exposure. J. Urol. 191:823-9, 2014. PMID:
24035882
341. Shah, G., Zhang, G., Chen, F., Cao, Y., KALYANARAMAN, B., See, W.A. iNOS expression and NO
production contribute to the direct effects of BCG on urothelial carcinoma cell biology. Urol. Oncol. 32:45.e1-9,
2014. PMID: 24054867
342. Jin H, Kanthasamy A, Ghosh A, Anantharam V, KALYANARAMAN, B., Kanthasamy AG. Mitochondriatargeted antioxidants for treatment of Parkinson's disease: Preclinical and clinical outcomes. Biochim. Biophys.
Acta 184:1282-94, 2014. [PMCID: PMC3961561; Available on 2015-08-01]
343. Zielonka, J., Lambeth, J.D., KALYANARAMAN, B. On the use of L-012, a luminol-based chemiluminescent
probe, for detecting superoxide and identifying inhibitors of NADPH oxidase: A re-evaluation. Free Radic. Biol.
Med. 65:1310-4, 2013. [PMCID: PMC4274999]
344. Srinivasan, S., Spear, J., Chandran, K., Joseph, J., KALYANARAMAN, B., Avadhani, N.G. Oxidative stress
induced mitochondrial protein kinase a mediates cytochrome C oxidase dysfunction. PLoS One 2013 Oct
10;8(10):e77129. doi: 10.1371/journal.pone.0077129. [PMCID: PMC3795003]
345. Michalski, R., Michalowski, B., Sikora, A., Zielonka, J., KALYANARAMAN, B. On the use of fluorescence
lifetime imaging and dihydroethidium to detect superoxide in intact animals and ex vivo tissues: A reassessment.
Free Radic. Biol. Med. 67:278-84, 2014. [PMCID: PMC4275029]
346. Koto, T., Michalski, R., Zielonka, J., Joseph, J., KALYANARAMAN, B. Detection and identification of
oxidants formed during •NO/O2•- reaction: A multi-well plate CW-EPR spectroscopy combined with HPLC
analyses. Free Radic. Res. 48:478-86, 2014. [PMCID: PMC4780754]
347. Mouradian, M., Kikawa, K.D., Dranka, B.P., Komas, S.M., KALYANARAMAN, B., Pardini, R.S.
Docosahexaenoic acid attenuates breast cancer cell metabolism and the Warburg phenotype by targeting
bioenergetic function. Mol. Carcinog. 54:810-20, 2015. PMID: 24729481
348. Kumar, A., Chen, S.H., Kadiiska, M.B., Hong, J.S., Zielonka, J., KALYANARAMAN, B., Mason, R.P.
Inducible nitric oxide synthase is key to peroxynitrite-mediated, LPS-induced protein radical formation in murine
microglial BV2 cells. Free Radic. Biol. Med. 73:51-9, 2014. [PMCID: PMC4111989; Available on 2015/8/1]
349. Cheng G, Zielonka J, McAllister D, Tsai S, Dwinell MB, KALYANARAMAN B. Profiling and targeting of
cellular bioenergetics: Inhibition of pancreatic cancer cell proliferation. Br. J. Cancer 111:85-93, 2014. [PMCID:
PMC4090735]
350. Zielonka J, Cheng G, Zielonka M, Ganesh T, Sun A, Joseph J, Michalski R, O'Brien WJ, Lambeth JD,
KALYANARAMAN B. High-throughput assays for superoxide and hydrogen peroxide: Design of a screening
workflow to identify inhibitors of NADPH oxidases. J. Biol. Chem. 289:16176-89, 2014. [PMCID: PMC4047388]
351. Hardy M, Poulhès F, Rizzato E, Rockenbauer A, Banaszak K, Karoui H, Lopez M, Zielonka J, Vasquez-Vivar
J, Sethumadhavan S, KALYANARAMAN, B, Tordo P, Ouari O. Mitochondrial targeted spin traps: synthesis,
superoxide spin trapping and mitochondrial uptake. Chem. Res. Toxicol. 27:1155-65, 2014. PMID: 24890552
352. Michalski R, Zielonka J, Gapys E, Marcinek A, Joseph J, KALYANARAMAN, B. Real time measurements
of amino acid and protein hydroperoxides using coumarin boronic acid. J. Biol. Chem. 289:22536-53, 2014.
[PMCID: PMC4139259]
353. Dranka BP, Gifford A, McAllister D, Zielonka J, Joseph J, O'Hara CL, Stucky CL, Kanthasamy AG,
KALYANARAMAN, B. A novel mitochondrially-targeted apocynin derivative prevents hyposmia and loss of
motor function in the leucine-rich repeat kinase 2 (LRRK2R1441G) transgenic mouse model of Parkinson's
disease. Neurosci. Lett. 583:159-64, 2014. [PMC4253647]
354. Pan J, Zhang Q, Liu Q, Komas SM, KALYANARAMAN, B, Lubet RA, Wang Y, You M. Honokiol inhibits
lung tumorigenesis through inhibition of mitochondrial function. Cancer Prev. Res. (Phila.) 7:1149-59, 2014.
PMID: 25245764
355. Shah G, Zielonka J, Chen F, Zhang G, Cao Y, KALYANARAMAN, B, See W. H2O2 Generation by bacillus
Calmette-Guérin induces the cellular oxidative stress response required for bacillus Calmette-Guérin direct effects
on urothelial carcinoma biology. J. Urol. 192:1238-48, 2014. PMID: 24928267
356. Smulik R, Debski D, Zielonka J, Michałowski B, Adamus J, Marcinek A, KALYANARAMAN, B, Sikora A.
Nitroxyl (HNO) reacts with molecular oxygen and forms peroxynitrite at physiological pH: Biological
implications. J. Biol. Chem. 289:35570-81, 2014. [PMCID: PMC4271240; Available on 2015/12/19]
357. Forman HJ, Augusto O, Brigelius-Flohe R, Dennery PA, KALYANARAMAN B, Ischiropoulos H, Mann GE,
Radi R, Jackson Roberts L 2nd, Vina J, Davies KJ. Even free radicals should follow some rules: A suggested guide
to free radical research terminology and methodology. Free Radic. Biol. Med. 78:233-5, 2014. PMID: 25462642
358. Zielonka J, Sikora A, Adamus J, KALYANARAMAN B. Detection and differentiation between peroxynitrite
and hydroperoxides using mitochondria-targeted arylboronic acid. Methods Mol. Biol. 1264:171-81, 2015.
[PMCID: PMC4451192; Available on 2016-01-01]
359. Zhang Q, Pan J, Lubet RA, Komas SM, KALYANARAMAN B, Wang Y, You M. Enhanced Antitumor
activity of 3-bromopyruvate in combination with rapamycin in vivo and in vitro. Cancer Prev. Res. (Phila.) 8:31826, 2015. PMID: 25644152
360. Cheng G, Zielonka J, McAllister D, Hardy M, Ouari O, Joseph J, Dwinell MB, KALYANARAMAN B.
Antiproliferative effects of mitochondria-targeted cationic antioxidants and analogs: role of mitochondrial
bioenergetics and energy-sensing mechanism. Cancer Lett. 365:96-106, 2015. [PMCID: PMC4476640; Available
on 2016-08-28]
361. Das S, Alhasson F, Dattaroy D, Pourhoseini S, Seth RK, Nagarkatti M, Nagarkatti PS, Michelotti GA, Diehl
AM, KALYANARAMAN B, Chatterjee S. NADPH oxidase-derived peroxynitrite drives inflammation in mice
and human nonalcoholic steatohepatitis via TLR4-lipid raft recruitment. Am. J. Pathol. 185:1944-57, 2015.
[PMCID: PMC4483465; Available on 2016-07-01]
362. Mao L, Liu YX, Huang CH, Gao HY, KALYANARAMAN B, Zhu BZ. Intrinsic Chemiluminescence
generation during advanced oxidation of persistent halogenated aromatic carcinogens. Environ. Sci. Technol.
49:7940-7, 2015. PMID: 26009932
363. Roy I, McAllister DM, Gorse E, Dixon K, Piper CT, Zimmerman NP, Getschman AE, Tsai S, Engle DD,
Evans D, Volkman BF, KALYANARAMAN B, Dwinell MB. Pancreatic cancer cell migration and metastasis is
regulated by chemokine-biased agonism and bioenergetic signaling. Cancer Res. 75:3529-42, 2015. [PMCID:
PMC4560104; Available on 2016-09-01]
364. Ghosh A, Langley MR, Harischandra D, Neal ML, Jin H, Anantharam V, Joseph J, Brenza T, Narsimhan B,
Kanthasamy A, KALYANARAMAN B, Kanthasamy AG. Mitoapocynin treatment protects againsts
neuroinflammation and dopaminergic neurodegeneration in a preclinical animal model of Parkinson's disease. J.
Neuroimmune Pharmacol. (in press, 2016).
365. Shah G, Zhang G, Chen F, Cao Y, KALYANARAMAN B, See WA. The Dose-Response Relationship of
BCG on Urothelial Carcinoma Cell Biology. J. Urol. 2015 Dec 13. pii: S0022-5347(15)05423-3. doi:
10.1016/j.juro.2015.11.073. [Epub ahead of print] PMID: 26694905
366. Zielonka J, Zielonka M, VerPlank L, Cheng G, Hardy M, Ouari O, Ayhan MM, Podsiadly R, Sikora A,
Lambeth JD, KALYANARAMAN B. Mitigation of NADPH Oxidase 2 Activity as a Strategy to Inhibit
Peroxynitrite Formation. J. Biol. Chem. 291:7029-44, 2016. [PMCID: PMC4807286; Available on 2017-03-25]
367. Dębski D, Smulik R, Zielonka J, Michałowski B, Jakubowska M, Dębowska K, Adamus J, Marcinek A,
KALYANARAMAN B, Sikora A. Mechanism of oxidative conversion of Amplex® Red to resorufin: Pulse
radiolysis and enzymatic studies. Free Radic. Biol. Med. 95:323-32, 2016. PMID: 27021961
368. Cheng G, Zielonka J, Ouari O, Lopez M, McAllister D, Boyle K, Barrios CS, Weber JJ, Johnson BD, Hardy
M, Dwinell MB, KALYANARAMAN B. Mitochondria-Targeted Analogues of Metformin Exhibit Enhanced
Antiproliferative and Radiosensitizing Effects in Pancreatic Cancer Cells. Cancer Res 76:3904-15, 2016. [PMCID:
PMC4930686; Available on 2017-07-01]
369. Zielonka J, Podsiadly R, Zielonka M, Hardy M, KALYANARAMAN B. On the use of peroxy-caged luciferin
(PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of
reaction intermediates and oxidant-specific minor products. Free Radic Biol Med 99:32-42, 2016.
370. Gao HY, Mao L, Li F, Xie LN, Huang CH, Shao J, Shao B, Kalyanaraman B, Zhu BZ. Mechanism of
Intrinsic Chemiluminescence Production from the Degradation of Persistent Chlorinated Phenols by the Fenton
System: A Structure-Activity Relationship Study and the Critical Role of Quinoid and Semiquinone Radical
Intermediates. Environ Sci Technol. 2017 Mar 07;51(5):2934-2943.
371. Zhao B, Qiang L, Joseph J, Kalyanaraman B, Viollet B, He YY. Mitochondrial dysfunction activates the
AMPK signaling and autophagy to promote cell survival. Genes Dis. 2016 Mar;3(1):82-87. PMCID: PMC5215801
372. Brenza TM, Ghaisas S, Ramirez JEV, Harischandra D, Anantharam V, Kalyanaraman B, Kanthasamy AG,
Narasimhan B. Neuronal protection against oxidative insult by polyanhydride nanoparticle-based mitochondriatargeted antioxidant therapy. Nanomedicine. 2017 Apr;13(3):809-820. PMCID: PMC5392427
373. Kalyanaraman B, Hardy M, Zielonka J. A Critical Review of Methodologies to Detect Reactive Oxygen and
Nitrogen Species Stimulated by NADPH Oxidase Enzymes: Implications in Pesticide Toxicity. Curr Pharmacol
Rep. 2016 Aug;2(4):193-201. PMCID: PMC5070669
Books, Chapters, and Reviews
1. KALYANARAMAN, B.: Detection of toxic free radicals in biology and medicine. In: Reviews in Biochemical
Toxicology, Vol. IV. (E. Hodgson, J.R. Bend and R.M. Philpot, eds.) Elsevier, 1982, pp. 73-139.
2. Mason, R. P., Harrelson, W. G., KALYANARAMAN, B., Mottley, C., Peterson, F. J., and Holtzman, J. L. Free
radical metabolites of chemical carcinogens. In: Free Radicals, Lipid Peroxidation and Cancer (D. C. H. McBrien
and T. F. Slater, eds.) Academic Press, New York, 1982, pp. 377-95.
3. KALYANARAMAN, B. and Sivarajah, K.: The ESR study of free radicals formed during the arachidonic acid
cascade and co-oxidation of xenobiotics by prostaglandin synthetase. In: Free Radicals in Biology, Vol. VI. (W.A.
Pryor, ed.) Academic Press, New York, 1984, pp. 149-98.
4. KALYANARAMAN, B., Felix, C. C., and Sealy, R. C.: Semiquinone anion radicals of catechol(amine)s,
catechol estrogens, and their metal ion complexes. Environ. Health Perspect. 64, 185-98 (1985).
5. Antholine, W. E., KALYANARAMAN, B., and Petering, D. H.: ESR of copper and iron complexes with
antitumor and cytotoxic properties. Environ. Health Perspect. 64, 19-35 (1985).
6. KALYANARAMAN, B. and Sinha, B. K.: Free radical-mediated activation of hydra-zine deriv¬atives. Environ.
Health Perspect. 64, 179-84 (1985).
7. KALYANARAMAN, B., Hintz, P., and Sealy, R. C.: An electron spin resonance study of free radicals from
catechol estrogens. Fed. Proc. 45, 2477-84 (1986). Review.
8. Hammel, K. E., KALYANARAMAN, B., and Kirk, T. K.: Oxidation of aromatic pollutants by P. chrysosporium
ligninase. In: Lignin: Enzymic and Microbial Degradation (E. Odier, ed.) Institut National de la Recherch
Agronomique, Paris, 1987, pp. 45-9.
9. Kersten, P. J., KALYANARAMAN, B., Hammel, K. E., and Kirk, T. K.: Horseradish peroxidase oxidizes
1,2,4,5 aetramethoxybenzene by a cation radical mechanism. In: Lignin: Enzymic and Microbial Degradation (E.
Odier, ed.) Institut National de la Recherch Agronomique, Paris, 1987, pp. 75-9.
10. Odlier, E., Mozuch, K., KALYANARAMAN, B., and Kirk, T. K.: Cellobiose quinone oxidoreductase does not
prevent oxidative coupling of phenols or polymerization of lignin by ligninase. In: Lignin: Enzymic and Microbial
Degradation (E. Odier, ed.) Institut National de la Recherch Agronomique, Paris, 1987, pp. 131-6.
11. KALYANARAMAN, B.: Free radicals from catecholamine hormones, neuromelanins and neurotoxins. In:
Handbook of Antioxidants and Free Radicals in Medicine, CRC Press, Boca Raton, FL, 1989.
12. Hodnick, W. F., KALYANARAMAN, B., and Pardini, R. S.: The production of hydroxyl and semiquinone
radicals during the autoxidation of redox active flavonoids. In: Oxygen Radicals in Biology and Medicine (M.G.
Simic, J.F. Ward, and K.A. Taylor, eds.) Plenum, New York, 1988, pp. 149-52.
13. Baker, J. E., Felix, C. C., and KALYANARAMAN, B.: Detection of free radicals by direct ESR during
myocardial cell injury - a critical evaluation. In: Oxy-Radicals in Molecular Biology and Pathology (P. Cerutti, I.
Fridovich, and J. McCord, eds.) Alan R. Liss, New York, 1988, pp. 343-51.
14. Baker, J., Felix, C. C., Olinger, G. N., and KALYANARAMAN, B.: ESR spectroscopy provides direct
evidence for free radical generation during myocardial ischemia and reperfusion. In: Free Radicals, Methodology
and Concepts (C. Rice-Evans and B. Halliwell, eds.) Richelieu, London, 1988, pp. 185-99.
15. KALYANARAMAN, B.: Characterization of o-semiquinone radicals in biological systems. Methods Enzymol.
186, 333-43 (1990).
16. KALYANARAMAN, B. and Feix, J. B.: The use of spin label oximetry in photody-namic studies. Spectrum 3,
9 (1990).
17. KALYANARAMAN, B., Antholine, W. E., Joseph, J., and Parthasarathy, S.: An ESR study of the oxidative
modification of low density lipoproteins. In: Oxidative Damage and Repair: Chemical, Biochemical, and Medical
Aspects (K. J. A. Davies, ed.) Pergamon Press, New York, 1991, pp. 154-9.
18. KALYANARAMAN, B., Darley-Usmar, V. M., and Parthasarathy, S.: Synergistic reaction betweeen probucolderived radical and ascorbic acid during oxidation of low-density lipoprotein. In: Active Oxygens, Lipid Peroxides,
and Antioxidants (K. Yaki, ed.) Japan Science Society Press, Tokyo, 1993, pp. 111-5.
19. Darley-Usmar, V. M., Hersey, A., Gotoh, N., Niki, E., Hogg, N., KALYANARAMAN, B., Stone, D., and
Wilson, M. T.: Lipid peroxidation in human low-density lipoprotein: interactions between antioxidants, transition
metals, and lipid hydroperoxides. In: Antioxidants, Free Radicals, and Polyunsaturated Fatty Acids in Biology and
Medicine (A. T. Diplock, J. M. C. Gutteridge, and V. K. S. Shukla, eds.) International Food Science Centre, 1993,
pp. 49-55.
20. KALYANARAMAN, B., Konorev, E. A., Joseph, J., and Baker, J. E.: Radical generation and detection in
myocardial reperfusion injury. In: Physical Methods in Biochemistry (C. Rice-Evans, ed.) Richelieu, London,
1993.
21. KALYANARAMAN, B.: Free radical mechanism of oxidative modification of low-density lipoprotein. In:
Oxidative Stress and Antioxidant Defenses in Biology (S. Ahmed, ed.) Chapman & Hall, 1994.
22. Darley-Usmar, V. M., Mason, R. P., Chamulitrat, W., Hogg, N., and KALYANARAMAN, B.: Lipid
peroxidation and cardiovascular disease. In: Immunopharmacology of Free Radical Species (P. Winyard, ed.)
Academic Press, New York, 1995, pp. 23-37.
23. KALYANARAMAN, B.: Thiyl radicals in biological systems: significant or trivial? Biochem. Soc. Symp. 61,
55-63, 1995. Review.
24. Favier, A. E., Cadet, J., KALYANARAMAN, B., Fontecave M., and Pierre, J. L. (eds.): Analysis of Free
Radicals in Biological Systems. Birkhäuser Verlag, Boston, 1995.
25. KALYANARAMAN, B.: Detection of nitric oxide by electron spin resonance in chemical, photo¬chemical,
cellular, physiological, and pathophysiological systems. In: Nitric Oxide, Part A - Sources (L. Packer, ed.)
[Methods Enzymol. 268] (1996), pp. 168-87.
26. Thomas C. and KALYANARAMAN, B. (eds.): Oxygen Radicals in Diseases. Gordon Publishers, U.K., 1997.
27. Darley-Usmar, V. M., Hogg, N., KALYANARAMAN, B., and Moore, K.: Free radicals in the vasculature. In:
Oxidative Stress, Lipoproteins, and Cardiovascular Dysfunction (Portand Press Research Monograph 7) (C. RiceEvans and K.R. Bruckdorfer, eds.), Portland Press, pp. 81-98, 1995.
28. Darley-Usmar, V. M., Moncada, S., Hogg, N., and KALYANARAMAN, B.: Lipid hydroperoxides and
atherosclerosis. In: International Congress Series (T. Yoshikawa, ed.) Excerpta Medica, International Congress
series 1058, Elsevier Science, pp. 509-12, 1994.
29. Hogg, N. and KALYANARAMAN, B. The use of nitric oxide gas in biological systems, in: Methods in
Molecular Biology, Vol. 100. Nitric Oxide Protocols (M. A. Titheradge, ed.), Totowa, NJ: Humana Press, pp. 2338, 1997.
30. V´asquez-Vivar, J.,Martsek, P., Hogg, N., Karoui, H., Masters, B. S. S., Pritchard, K. A. Jr., and
KALYANARAMAN, B.: Electron spin resonance spin-trapping detection of superoxide generated by neuronal
nitric oxide synthase. In: Nitric Oxide, Part C: Biological and Antioxidant Activities (L. Packer, ed.) [Methods
Enzymol. 301] (1999), pp. 169-77. Review.
31. Goss, S. P. A., KALYANARAMAN, B., and Hogg, N.: Antioxidant effects of nitric oxide and nitric oxide
donor compounds on low-density lipoprotein. In: Nitric Oxide, Part C: Biological and Antioxidant Activities (L.
Packer, ed.) [Methods Enzymol. 301: 444-53, 1998. Review.]
32. KALYANARAMAN, B., Joseph, J., Kalivendi, S., Wang, S., Konorev, E., Kotamraju, S.: Doxorubicininduced apoptosis: implications in cardiotoxicity. In: Microcompetition with Foreign DNA and the Origin of
Chronic Disease. [Mol. Cell. Biochem. 234-235 (1-2):119-24, 2002. Review.]
33. Kotamraju, S., Kalivendi, S.V., Konorev, E., Chitambar, C.R, Joseph, J., KALYANARAMAN, B. Oxidantinduced iron signaling in doxorubicin-mediated apoptosis. Methods Enzymol. 378:362-82, 2004. Review.
34. KALYANARAMAN, B. Introduction to the Review series "Protein Radicals in Biological Systems - ESR Spin
Trapping and Immuno Spin Trapping Approaches". Free Radic. Biol. Med. 36:1071, 2004.
35. KALYANARAMAN, B. Introduction to the Review Series on redox-active metal ions, reactive oxygen species
and apoptosis. Free Radic. Biol. Med. 37:573, 2004.
36. Vásquez-Vivar, J., Martásek, P., KALYANARAMAN, B. Superoxide generation from nitric oxide synthase.
Role of cofactors and protein-protein interaction. Biological Magnetic Resonance, Volume 23, Biomedical EPR Part A: Free Radicals, Metals, Medicine, and Physiology, (S. S. Eaton, G. R. Eaton, and L. J. Berliner, eds.),
Kluwer Academic/Plenum Publishers, New York, 75-91 (2005).
37. Zhang, H., Joseph, J., KALYANARAMAN, B. Hydrophobic tyrosyl probes for monitoring nitration reactions
in membranes. Methods Enzymol. 396:182-204, 2005.
38. Kotamraju, S., Kalivendi, S., Shang, T., KALYANARAMAN, B. Nitric oxide, proteasomal function, and iron
homeostasis - implications in aging and neurodegenerative diseases. Methods Enzymol. 396:526-34, 2005.
39. KALYANARAMAN, B. Iron signaling and oxidant damage. Cardiovasc.Toxicol. 7:92-4, 2007. Review.
40. Zhang, H., Xu, Y., Joseph, J., KALYANARAMAN, B. Influence of intramolecular electron transfer
mechanism in biological nitration, nitrosation, and oxidation of redox-sensitive amino acids. Methods Enzymol.
440:65-94, 2008. Review.
41. Bartesaghi, S., Peluffo, G., Zhang, H., Joseph, J., KALYANARAMAN, B., Radi, R. Tyrosine nitration,
dimerization, and hydroxylation by peroxynitrite in membranes as studied by the hydrophobic probe N-t-BOC-1tyrosine tert-butyl ester. Methods Enzymol. 441C:217-36, 2008.
42. Zielonka, J., KALYANARAMAN, B. "ROS-generating mitochondrial DNA mutations can regulate tumor cell
metastasis" - A critical commentary. Free Radic. Biol. Med. 45:1217-9, 2008. [PMCID: 3595710]
43. Janssen-Heininger, Y.M., Mossman, B.T., Heintz, N.H., Forman, H.J., KALYANARAMAN, B., Finkel, T.,
Stamler, J.S., Rhee, S.G., van der Vliet, A. Redox-based regulation of signal transduction: principles, pitfalls, and
promises. Free Radic. Biol. Med. 45:1-17, 2008. Review. [PMCID: PMC2453533]
44. Subramanian, S., KALYANARAMAN, B., Migrino, R.Q. Mitochondrially targeted antioxidants for the
treatment of cardiovascular diseases. Recent Pat. Cardiovasc. Drug Discov. 5:54-65, 2010. Review.
45. Zielonka, J., KALYANARAMAN, B. Hydroethidine- and Mito-SOX-derived red fluorescence is not a reliable
indicator of intracellular superoxide formation: Another inconvenient truth. Free Radic. Biol. Med. 48:983-1001,
2010. Review. [PMCID: PMC35871542]
46. KALYANARAMAN, B. Oxidative chemistry of fluorescent dyes: Implications in the detection of reactive
oxygen and nitrogen species. Biochem. Soc. Trans. 39:1221-5, 2011. Review. PMID: 21936793
47. Zielonka, J., KALYANARAMAN, B. Methods of investigation of selected radical oxygen/nitrogen species in
cell-free and cellular systems. In: Principles of Free Radical Biomedicine, Volume 1, K. Pantopoulos and H.M.
Schipper (eds). Nova Science Publishers, 2011.
48. Zielonka, J., Joseph, J., Sikora, A., KALYANARAMAN, B. Real-time monitoring of reactive oxygen and
nitrogen species in a multiwell plate using the diagnostic marker products of specific probes. Methods Enzymol.
526:145-57, 2013. PMID: 23791099
49. Debowska, K., Debski, D., Hardy, M., Jakubowska, M., KALYANARAMAN, B., Marcinek, A., Michalski, R.,
Michalowski, B., Ouari, O., Sikora, A., Smulik, R., Zielonka, J. Toward selective detection of reactive oxygen and
nitrogen species with the use of fluorogenic probes - Limitations, progress, and perspectives. Pharmacol. Rep.
67(4):756-64, 2015. Review. PMID: 26321278
50. KALYANARAMAN, B., Hardy, M., Zielonka, J. A critical review of methodologies to detect reactive oxygen
and nitrogen species by NADPH oxidase enzymes: implications in pesticide toxicity. Curr. Pharmacol. Rep. (in
press, 2016). [NIHMS: 792899]
Editorials, Letters to Editor, Other
1. Zielonka, J., Sikora, A., Joseph, J., KALYANARAMAN, B. Reply to Jourd'heuil: Peroxynitrite is the major
species formed from different flux ratios of co-generated nitric oxide and superoxide. J. Biol. Chem. 285:le16,
2010. [PMCID: PMC2930751]
Non-Peer Reviewed Educational Products
1. KALYANARAMAN, B. (2007), "Chemistry and biochemistry of molecular probes used in the detection of
reactive oxygen and nitrogen species", in Davies, K. (ed.), Free Radicals and the Oxygen Paradox: Oxidative Stress
in Biology, Aging and Disease, The Biomedical & Life Sciences Collection, Henry Stewart Talks Ltd., London
(online at http://hstalks.com/bio and http://hstalks.com/lib.php?t=HST28.1682_1_2&c=252)
Database, Video, or Other Research/Clinical Contributions
1. U.S. Patent No. 6,589,751 (issued July 8, 2003), “Immunological detection of the homocystamide adduct and a
thiolactone immunoassay for endogeneous homocysteine” Inventors: Eric Ferguson, Sampath Parthasarathy, and
Balaraman Kalyanaraman
2. U.S. Patent No. 7,223,864 (issued May 29, 2007), “2-hydroxyethidium, methods of preparation and uses
thereof” Inventors: Balaraman Kalyanaraman and Hongtao Zhao
3. U.S. Patent No. 8,143,420 (issued March 27, 2012), “Bifunctional and trifunctional nitrone spin trapping
compounds and uses thereof” Inventors: Micael Hardy, Neil Hogg, Balaraman Kalyanaraman, Marcos Lopez,
Olivier Ouari, and Paul Tordo
4. U.S. Patent No. 8, 388,931 (issued March 5, 2013) “99mTc-labeled triphenylphosphonium derivative contrasting
agents and molecular probes for early detection and imaging of breast tumors” Inventors: Balaraman
Kalyanaraman, Micael Hardy, Marcos Lopez, and Ming Zhao
5. U.S. Patent No. 8,388,936 (issued March 5, 2013), “In vivo mitochondrial labeling using positively-charged
nitroxide enhanced and gadolinium chelate enhanced magnetic resonance imaging” Inventors: Balaraman
Kalyanaraman, Joy Joseph, Kathleen Schmainda, Douglas Prah, Marcos Lopez, and Micael Hardy
6. U.S. Patent No. 8,466,130 (issued June 18, 2013) “Mitochondria targeted cationic anti-oxidant compounds for
prevention, therapy or treatment of hyper-proliferative disease, neoplasias and cancers” Inventors: David A.
Zarling, Hirak S. Basu, Balaraman Kalyanaraman, and Joy Joseph
7. U.S. Patent No. 8,962,600 (issued February 24, 2015), “Neuroprotective compounds and their use” Inventors:
Balaraman Kalyanaraman, Joy Joseph, and Anumantha Kanthasamy