By Dr Anitha Learning objectives : At the end of lectures on this topic, I MBBS students should be able to: • Explain what are nucleosides & nucleotides • Describe the composition, structure of DNA, based on the Watson-Crick model and explain the function of DNA. • List the different types of RNA, describe the composition, structures and explain the function of each. 2 Nucleic acids are Macromolecules present in all cells. Both eukaryotes and prokaryotes Present mostly in nucleus but also in cytoplasm and mitochondria Important for protein synthesis and inheritance Codes genetic information Takes part in cell division Polymers of monomeric units called NucleotidesPolynucleotides Two types – Based on the sugar present Deoxyribonucleic Acid (DNA) Transcription DNA Replication DNA Ribonucleic Acid (RNA) Translation RNA Protein Location – DNA – Nucleus & Mitochondria(small amount) 4 RNA –Cytoplasm (90%) & Nucleolus • Building blocks of Nucleic acids • Composed of 1. Sugar 2. Nitrogenous Base 3. phosphate group • Phosphate esters of 5 carbon sugar (Pentose) in which a nitrogenous base is covalently linked to C1 of sugar residue • They are phosphorylated nucleosides Nucleotide structure 5′ 7 a) Nitrogenous base 2 Major types found in nucleotides: 8 Purine bases 6- amino purine 2 – amino ,6-oxo purine 9 Pyrimidine bases in RNA in DNA & RNA in DNA except tRNA 10 Minor bases (modified bases) found in nucleotides in small amounts: Examples: • 7-methylguanine • 5-methylcytosine • Dihydrouracil (DHU) Unusual bases help in recognition by specific enzymes or protect from being degraded by nucleases . 11 • Bases occurring freely as metabolic intermediates Examples: Xanthine Hypoxanthine Uric acid (end product of purine catabolism) • Bases present in plants Examples: Caffeine (of coffee) Theophylline (of tea) Theobromine [of cocoa] 12 b) Pentose sugars present in nucleotides, RNA and DNA Ribose in RNA 2-deoxy ribose in DNA 13 c) Phosphate group • is attached to pentose sugar (5′ hydroxyl group) by an ester linkage in a nucleotide • 1, 2 or 3 phosphate group/s may be present in a nucleotide 14 Nucleoside It consists of: Nitrogenous base Sugar (purine/pyrimidine) N-glycosidic bond • Ribonucleoside contains ribose sugar • Deoxyribonucleoside contains deoxy ribose sugar 15 Examples Base Ribonucleoside Adenine Guanine Cytosine Uracil Thymine Adenosine (Adenine + Ribose) Guanosine (Guanine + Ribose) Cytidine (Cytosine + Ribose) Uridine (Uracil + Ribose) - Deoxyribonucleoside Deoxyadenosine (Adenine + Deoxyribose) Deoxyguanosine (Guanine + Deoxy ribose) Deoxycytidine (Cytosine + Deoxyribose) Thymidine (Thymine + Deoxyribose) 16 Nucleotide • is phosphorylated nucleoside Nitrogenous base Sugar Phosphate/s N-glycosidic bond ester linkage • can exist as -Nucleoside monophosphate (NMP), -Nucleoside diphosphate (NDP) or as ( 1 high energy bond ) -Nucleoside triphosphate (NTP)(2 high energy bonds 17 Examples: Ribonucleotide Deoxyribonucleotide AMP (adenosine + phosphate) GMP (guanosine + phosphate) CMP (cytidine + phosphate) UMP (uridine + phosphate) - dAMP (deoxy adenosine + phosphate) dGMP (deoxy guanosine + phosphate) dCMP (deoxy cytidine + phosphate) TMP (thymidine + phosphate) 18 Biologically important free Nucleotides & their functions Functions Building blocks of nucleic acids : DNA RNA Sources of energy Examples of nucleotides dAMP, dGMP, dCMP, TMP AMP, GMP, CMP, UMP ATP (universal energy currency), GTP Second messengers cyclic AMP (cAMP) (FSH, LH,TSH,Corticotropin, Vasopressin, Glucagon ,PTH), cGMP Donors of certain groups Phosphoadenosine phosphosulfate PAPS S-Adenosyl methionine (SAM) UDP glucose CDP choline + 19 Synthetic analogues of Purine, Pyrimidine Nucleotides: Therapeutic uses Anticancer agents Examples 6-mercaptopurine, 5- fluorouracil Immunosupressants Azathioprine Treatment of gout Allopurinol Antiviral agents 20 Answer this: Which of the following nucleotides YOU functions as a THANK second messenger? a. FAD b. PAPS c. dGMP d. cAMP Genetic material in both Prokaryotes and Eukaryotes Prokaryotes Lack separation from cytoplasm Absence of nuclear membrane Not organised with proteins Eukaryotes Separate from cytoplasm Nuclear membrane present Organised with proteins Chromatin Less than 0.1% is present in Mitochondria • Definition DNA is a polymer of deoxyribonucleotides (i.e. it is a polynucleotide). 24 • Location Mitochondria: Circular DNA Nucleus: Linear DNAchromosomes 25 Watson & Crick 1953 First described the structure of DNAA milestone in modern biology (Noble Prize 1962) 26 Structure of DNA: Watson and Crick model Salient features: • DNA is double-stranded • Each DNA strand has a linear arrangement of its four nucleotides (monomeric units); dAMP (A), dGMP (G), dCMP (C) and TMP (T) 27 Primary structure of each DNA strand • It is the sequence of deoxyribonucleotides in the polynucleotide chain E.g. A-G-T-C…….. 28 Nucleotide 1 P 3’, 5’ Phospho diester P bond Deoxy ribose 3′ end sugar 5′ end Base, A Nucleotide 2 Deoxy ribose sugar Base, G 29 Watson and Crick model 5′- OH Phospho diester bonds Strand 1 Strand 2 H bonds (2) 3′- OH H bonds (3) 30 Backbone formed by Sugar &Phosphate Core by Nitrogenous bases Two polar ends – 3’ & 5’ ends Inspired by DNA….!! Spiral staircase 32 DNA contains two polynucleotide chains B-DNA Polynucleotide chains have 5’ end and 3’ end Two polynucleotide chains are coiled round a common axis Two polynucleotide chains are antiparallel Right handed double helix Sugar and phosphate forms the backbone - Hydrophilic 33 Purine and pyrimidine bases are Hydrophobic Purines – A& G Pyrimidines –C&T Purine and pyrimidine bases present inside the core – (STACKED) B-DNA Has got Major groove and Minor groove Where proteins bind to DNA 35 Secondary structure of DNA • It refers to the coiling of double-stranded DNA molecule • Coiling is right- handed Right handed double helix of DNA 36 H bonds hold the two strands in a double-helix structure Adenine base pairs with Thymine forming two hydrogen bonds A=T Guanine base pairs with Cytosine forming three hydrogen bonds G=C B-DNA 37 Hydrogen bonds The amount of Adenine = Thymine Guanine = Cytosine The amount of Purines = Pyrimidines Chargaff’s Rule B-DNA 38 Diameter of the double helix is 20A0 One turn of double helical DNA travels a distance of 34 A0 One turn contains 10 base pairs Distance between the adjacent nucleotides is 3.4 A0 B-DNA 39 Learning check point: Which of the following statements on DNA double helical structure is true? a. The two strands run in parallel direction b. The two strands are held together by phosphodiester bonds c. H bonds are formed between complementary base pairs d. The strands are composed of ribonucleotides B-DNA-Most common Right – handed 10 base pairs per one full turn Z-DNA Left – handed 12 base pairs per one full turn A-DNA Right – handed 11 base pairs per one full turn 41 25 A0 34A0 45 A0 12 base pairs 10 base pairs 11 base pairs • Linear DNA double stranded structure – Human DNA • Circular DNA – Bacteria – no free ends • Length – 1.74 meters when stretched • Made compact to fit into nucleus- Supercoiling • Supercoils – Right handed (positive) Left handed (Negative)-common • In prokaryotes – circular DNA with RNA protein core • In Eukaryotes more complicated • 10000 fold shortening by supercoiling is seen to fit into nucleus as chromosomes (23 pairs) • During resting state – Chromatin Cell division- Chromosomes • A typical human cell contains DNA which is approximately two meters long. ??? How do you get 2 m of DNA into a 6 µm nucleus of a cell? 45 Structural units of chromatin composed of DNA (negatively charged)bound to histones (positively charged) forming octomers DNA winds around the core of histones DNA links between two nucleosomes continuously Core Histone H1 Histone DNA 47 Neighboring Nucleosomes are joined by ‘linker’DNA (about 30 base pair long) H1 Histone binds to linker DNA Helps to pack nucleosomes into a more compact structure Core Histone DNA It resembles beads on a string H1 Histone 48 •10-nm fibril is probably further supercoiled •Six or seven nucleosomes per turn to form the 30-nm chromatin fiber DNA Protein scaffolding 1400 nm Nucleosome 10nm 30 nm 300 nm Looped domains 700 nm 49 Nucleosomes Loops of chromatin 50 DR. ASHOK KUMAR J. 51 52 Genome 46 chromosomes (3 billion base pairs) 53 Function of DNA • DNA contains genetic information • The sequence of four nucleotides (A, T, G, C) in DNA encodes genetic information. • The code is read by copying stretches of DNA into RNA by transcription. • In DNA, about 10% is coding region and non-coding region is about 90%. 54 • Coding regions in DNA code for proteins, RNA. • Structural genes code for proteins; other THANK YOU genes code for RNA. • The strand of DNA containing the code for the protein is called Template strand; opposite strand is Coding strand. 55 Flow of genetic information DNA RNA Transcription Central Dogma of Molecular Biology Protein Translation Phenotype (Physical characteristics) 56 Melting of DNA Annealing 57 Differences between RNA and DNA RNA DNA Seen in: cytoplasm, nucleus Single stranded Sugar is ribose Usually has: 100-5000 bases Pyrimidine: Uracil Chargaff’s rule: not obeyed Destroyed by alkali Function: Protein synthesis Nucleus, mitochondria Double stranded Sugar is deoxyribose Millions of base pairs Thymine Obeys Chargaff’s rule Alkali-resistant Contains genetic information; basis for heredity and evolution58 RNA (Ribonucleic acid) • Definition: RNA is a polymer of ribonucleotides joined by 3′-5′ phosphodiester bonds and generally single-stranded. • RNA is involved in protein synthesis • RNA is transcribed from genes present in DNA 59 Types of RNA 1. 2. 3. 4. Messenger RNA (mRNA) Transfer RNA (tRNA) Ribosomal RNA(rRNA) Small nuclear RNA(snRNA) 60 1. Messenger RNA (mRNA) • Mature mRNA is produced from heteronuclear RNA (hnRNA) which is transcribed from the DNA in nucleus. • hnRNA is processed to form mRNA. 61 Nucleus Cytoplasm Protein mRNA 62 Structure of mRNA Codons 63 prevents the hydrolysis of mRNA by 5′ exonucleases mRNA Translated region contains codons for amino acids 7- methylguanosine triphosphate polymer of adenylate nucleotides (20-250) maintains intracellular stability of mRNA 64 Function of mRNA: • It acts as a messenger, and conveys genetic information from DNA to protein synthesizing machinery in cytoplasm 65 2. Transfer RNA (tRNA) • At least 20 different types tRNAs exist (at least one for each amino acid). • Length is 74 -95 nucleotides • (It was formerly called soluble RNA or sRNA). 66 Structure of tRNA It resembles a clover leaf. It has 4 arms: 1) Acceptor arm 2) Anticodon arm 3) D arm 4) TC arm ( = psi) The arms have base-paired stems and unpaired loops (no loop for acceptor arm). 67 Attachment site for the amino acid Recognition site for enzyme that adds the amino acid Recognition of triplet codon of mRNA tRNA contains some unusual bases Involved in binding of tRNA to ribosome 68 Function of tRNA: • It is an adaptor molecule for the translation of codons in mRNA into protein sequence. • It does so by transporting (carrying) amino acid to the site of protein synthesisribosomes. Amino acid Ribosomes - mRNA tRNA 69 Met tRNA Anticodon UAC Codon mRNA 70 3. Ribosomal RNA (rRNA) • rRNA and proteins complex to form Ribosomes (nucleoproteins) which are sites of protein synthesis. 71 Eukaryotic Ribosome Small subunit 40 S 18S rRNA + 33 proteins 80 S Large subunit 60 S 5S, 28S, 58S rRNAs + 49 proteins S = Svedberg units 72 Function of rRNA • rRNA is necessary for binding of mRNA to the ribosome. • 28s rRNA of 60S subunit has peptidyl transferase activity for peptide bond formation (i.e. it is a Ribozyme; RNA as an enzyme). 73 4. Small nuclear RNA (snRNA) • Their sizes range from 90-300 nucleotides. • They are named: U1, U2, U4, U5, U6 and U7 (rich in Uracil) • They are examples for Ribozymes. Function • Involved in processing of mRNA (splicing) and • Gene regulation 74 Learning check point: The codon is found on _________ , and the anticodon is found on ________ . A. rRNA, mRNA B. mRNA, tRNA C. tRNA, mRNA D. mRNA, rRNA 75 An illustration of the functions of different RNAs DNA T R A N S C R I P T I O N hnRNA Splicing mRNA Contains codons for protein synthesis snRNA Forms rRNA Ribosome TRANSLATION Proteins tRNAs Amino acids Aminoacyl-tRNAs Transports Amino acids Proteins 76 You have successfully completed learning the topic: Chemistry of Nucleotides and Nucleic acids 77 Reach the top! 78
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