Chapter 7 Cellular Structure and Function The Cell Theory • All organisms are composed of one or more cells • The cell is the basic unit of structure and organization of organisms. • All cells come from preexisting cells. Advent of the Cell • Hooke and Leeuwenhoek used early microscopes – Hooke saw/named “cells” in dead cork – Leeuwenhoek saw living cells in pond water Light Microscopes • Use 1 or more lenses and visible light • Can view live specimens • Max 2000x due to poor resolution Electron Microscopes • Uses electrons instead of light for image • Very high power/resolution ( max 2 million X) • Specimen prep is deadly • SEM = Scanning, 3d surface image • TEM = Transmission, 2d, more powerful, view slice Microscopy Links http://biologygmh.com/ 9560x Cell Types Prokaryotes Eukaryotes • Simple (small) • More complex (larger) – Plasma membrane – Plasma membrane – Cytosol – Cytosol – Non-membrane bound organelles – Non-membrane bound organelles – No membrane bound organelles – Has membrane bound organelles • Nucleus Plasma Membrane • Cell boundary (inside vs. environment) • Phospholipid Bilayer • Selectively permeable – Controls what/how much can enter or leave cell – Allows waste out/nutrients in Phospholipid Bilayer • Phospholipid structure – 2 layers, tails facing each other – Glycerol + phosphate polar “Head” – 2 nonpolar fatty acid tails (sat or unsat) The Fluid Mosaic Model • Phospholipid flow • Other molecules float on/with lipids – Proteins – Carbs – Cholesterol Membrane Proteins • Signal transmission • Transport • Support Membrane Carbohydrates • Attached to protein or lipid • Convey signals/communication Membrane Cholesterol • Embedded into phospholipids • Increase membrane fluidity – Especially in low/high heat – Unsat fat also helps with low temp Cell Structures and Organelles Animal Cell Plant Cell Cellular Pursuit 7.3 Structures and Organelles 7.3 Structures and Organelles Outer Cell Structures • Cell Membrane – All cells have it – Flexible Phospholipid bilayer w/proteins – Selectively permeable (controls in/out) • Cell Wall – Not found in animal cells – Inflexible barrier for cell protection and support Outer Structures Cont. • Flagella – Long whiplike projections on some cells – Used for movement or feeding • Cilia – Small numerous cell projections on some cells – Used for movement or feeding Some other major structures • Nucleus – Enclosed by double membrane nuclear envelope – Envelope has pores for transport – Contains DNA and nucleoli • Ribosomes – Protein synthesis – Made by nucleoli – No membrane (all cells have them) Energy Organelles • Mitochondria – In all eukaryotic cells – Site of cell respiration (ATP production, Energy!) – Has folded inner membrane • Chloroplast – Only in Plant/algae cells – Site of photosynthesis (organic molecule creation) – Has lots of small sacs stacked inside A Cell Framework • Cytoskeleton – Protein skeleton – Provides structure, support, transport • Centrosome/Centrioles – microtubule production (ex. cell reproduction) – Centrioles only in animal cells Endomembrane System • Endoplasmic Reticulum (ER) – Highly folded membranes • Smooth ER: lipid synthesis • Rough ER (w/ribosomes): protein synthesis • Transport and detox • Golgi Apparatus – Flattened stack of membranes • Modifies, packages, export proteins Endomembrane System II • Vacuoles – Membrane bound storage vesicles – Reduced or not present in animal cells – Very large central vacuole in plants • Lysosomes – Membrane bound digestive vesicle – Contain enzymes – Usually only in animal cells Cellular Transport • Passive Transport (uses no Energy) – Diffusion – Osmosis – Facilitated Diffusion Diffusion • Move from high to low conc. – “Down” the conc. Gradient – Affected by temp, pressure, conc. – Dynamic Equilibrium = no net movement Initial Conditions Diffusion Low High High Low Osmosis • Diffusion of water across membrane – Water is bound by solutes – More solute = less water – Water moves from low solute to high solute – Solutes suck Osmosis II • Three osmotic conditions – Isotonic: equal solute/water, no net movement – Hypertonic: more solute (water enters) – Hypotonic: less solute (water leaves) – Pay attention to frame of reference • Ex. The aquarium fish cells are hypertonic to the water can also be the aquarium water is hypotonic to the fish cells Osmosis III • You find yourself stranded in a ship at sea. There is no rescue in sight, and you’ve already eaten Ra (2 hours after being stranded). Should you drink the seawater or go without water? • The zombie invasion has begun. You avoid Luckee (smart move, even if she’s not a zombie) by ducking into a bathroom. Is it a good idea to sleep in the bathtub full of water (assuming you won’t drown)? Facilitated Diffusion • Diffusion through a membrane protein • Effects molecules unable to pass through membrane without help: – Larger and polar – Charged (ions) Diffusion in a Cell Channel Proteins Carrier Proteins Active Transport • All move from low to high conc. • All use Energy (ATP) • 3 types: – Protein pumps – Endocytosis – Exocytosis Sodium Potassium Pump • Sample protein pump • Pumps 3 Na+ out of cell; 2 K+ into cell – Uses ATP, makes electrochemical gradient • Gradient of charge and chemicals Endo/Exocytosis • Endocytosis: – Ingest large molecules or small cells – Envelope particles with cell membrane • Exocytosis – Release large molecules/waste – Merge vesicle with cell membrane 7.1 Formative Questions Robert Hooke and Anton van Leeuwenhoek designed microscopes that enabled them to see organelles within the cells they observed. A. true B. false 1. A 2. B 7.1 Formative Questions Which type of electron microscope directs electrons over the surface of a nonliving specimen, producing a three-dimensional image? A. transmission electron microscope B. scanning electron microscope C. scanning tunneling electron microscope 1. D. atomic force microscope A 2. B 3. C 4. D 7.1 Formative Questions All cells, whether eukaryotic or prokaryotic, have a plasma membrane. A. true B. false 1. A 2. B 7.1 Formative Questions What feature of eukaryotic cells has enabled them to develop more specific functions than prokaryotic cells? A. nucleus B. organelles C. genetic material (DNA) D. larger cell size 1. A 2. B 3. C 4. D 7.2 Formative Questions Which term describes the function of proteins found on the outer surface of the plasma membrane? A. identifiers B. receptors C. supporters D. transporters 1. A 2. B 3. C 4. D 7.2 Formative Questions Which component of the plasma membrane contributes to the fluidity of the plasma membrane? A. phospholipids B. proteins C. carbohydrates D. cholesterol molecules 1. A 2. B 3. C 4. D 7.2 Formative Questions What is the function of carbohydrates that stick out from the outer surface of the plasma membrane? A. They give the cell shape and enable it to move. B. They give the plasma membrane fluidity. C. They help the cell identify chemical signals. 1. A D. They hold the cell in place. 2. B 3. C 4. D 7.3 Formative Questions In prokaryotic cells, where does the conversion of fuel particles into usable energy occur? A. cytoplasm B. DNA C. mitochondria D. ribosomes 1. A 2. B 3. C 4. D 7.3 Formative Questions Which organelle is similar to the section of a factory that organizes, boxes, and ships the final product? A. chloroplast B. endoplasmic reticulum C. Golgi apparatus D. mitochondrion 1. A 2. B 3. C 4. D 7.3 Formative Questions In which structure is light energy captured and converted to chemical energy? A. chloroplast B. lysosome C. nucleus D. vacuole 1. A 2. B 3. C 4. D 7.4 Formative Questions Which type of cellular transport does not require an input of energy? A. active transport B. endocytosis C. exocytosis D. facilitated diffusion 1. A 2. B 3. C 4. D 7.4 Formative Questions How does osmosis occur between the two solutions separated by the selectively permeable membrane? A. Sugar moves to the left. B. Sugar moves to the right. C. Water moves to the left. D. Water moves to the right. 1. A 2. B 3. C 4. D 7.4 Formative Questions Which diagram shows a cell in a hypotonic solution? A. B. C. 1. A 2. B 3. C Chapter 7 Cellular Structure and Function Chapter Assessment Questions Compare and contrast plant and animal cells. Answer: Plant cells have both cell walls and chloroplasts. Animal cells do not have these structures. Only animal cells contain lysosomes. Both plant and animal cells have plasma membranes, a nucleus, mitochondria, vacuoles, endoplasmic reticulum, Golgi apparatus, centrioles, a nucleolus, ribosomes, and a cytoskeleton. Chapter 7 Cellular Structure and Function Chapter Assessment Questions Identify the structure represented by this image. Answer: plasma membrane and phospholipid bilayer Chapter Diagnostic Questions If you wanted to see a detailed 3-dimensional image of a cell, which type of microscope would you use? A. scanning electron microscope B. transmission electron microscope C. scanning tunneling microscope D. simple light microscope 1. A 2. B 3. C 4. D Chapter Diagnostic Questions Which is not a part of the cell theory? A. Cells are the basic unit of structure and organization of all living things. B. All living things are composed of one or more cells. C. Cells arise only from previously existing cells. D. Cells are produced spontaneously as an 1. A organism grows. 2. B 3. C 4. D Chapter Diagnostic Questions Which cell organelles are directly involved with the production of proteins? A. ribosomes, endoplasmic reticulum, nucleus B. cell membrane, lysosome, Golgi apparatus C. nucleus, centriole, vacuole D. endoplasmic reticulum, cilia, mitochondrion 1. A 2. B 3. C 4. D Chapter Assessment Questions _______ is the net movement of particles from an area where there are many particles of the substance to an area where there are fewer particles of the substance. A. Diffusion B. Endocytosis C. Exocytosis D. Equilibrium 1. A 2. B 3. C 4. D Standardized Test Practice What is the magnification of a compound light microscope that uses a 10X lens in series with a 25X lens? A. 2.5X B. 25X C. 35X D. 250X 1. A 2. B 3. C 4. D Standardized Test Practice What part of the plasma membrane makes it difficult for water-soluble substances to move freely into and out of the cell? A. membrane proteins B. transport proteins C. the nonpolar tails in the middle of the 1. plasma membrane D. the polar heads facing the inside and2. 3. outside of the cell 4. A B C D Standardized Test Practice Muscle cells require a great amount of energy. Which would you expect to find in large numbers in muscle cells? A. centrioles B. endoplasmic reticulum C. mitochondria D. ribosomes 1. A 2. B 3. C 4. D Standardized Test Practice In what type of cell would you most likely expect to find numerous lysosomes? A. a plant cell that stores food, enzymes, or other materials B. a plant cell that uses light energy for photosynthesis 1. A C. a single-celled organism that moves around its environment 2. B D. a white blood cell that digests bacteria 3.and C 4. D viruses Standardized Test Practice Why are the carrier proteins that move substances across a plasma membrane from a region of higher concentration to a region of lower concentration called pumps? A. They require energy to move substances against a concentration gradient. B. They open and close to allow substances to diffuse across the plasma membrane. C. They help with the osmosis of water through the 1. A plasma membrane. B D. They pump water into the cell, causing the2.pressure 3. C within the cell to increase. 4. D
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