Annexure ‘CD – 01’ FORMAT FOR COURSE CURRICULUM Course Title: Plant - Microbe Interaction L T P/S SW/ FW 2 0 2 2 Course Code: MBT704 TOTAL CREDIT UNITS 04 Credit Units: 03+01 Course Objectives: To apprise the students of the synergistic and antagonistic systems and mechanisms of interaction between microbes and in plants beneficial to humans. Pre-requisites: Basic knowledge of Life Sciences Student Learning Outcomes: Describe the systems and the nature of plant microbe interactions. Define the mechanisms of beneficial interactions and explain the ways of taking the best advantages from the interactions and the future prospects, and Design the system and mechanism of harmful interactions and the ways to mitigate the harm in a sustainable manner and the future prospects. Appraise the role of microbes in soil health improvement Course Contents/Syllabus: Module I An introduction to plant microbe interactions and their evolution Types and nature of various interaction like symbiosis, parasitism, mutualism, commensalisms, saprophytism, and necrotrphism The concept of microbe plant micro-ecological spheres like rhizosphere, phyllosphere and endosphere Module II Plant disease etiology, epidemiology, development process (morpho-physiolological, biochemical and molecular) and management Introduction to the concept of integrated disease management (A few appropriate and important microbial diseases will be employed as examples to explain various systems) Module III Types and mechanisms of disease resistance: general and specific to pathogens Introduction to molecular basis of resistance. (A few appropriate and important microbial diseases will be employed as examples to explain various systems) Module IV Rhizospheric microbes: associative, free living, symbiotic (including nitrogen fixers) The nature and mechanism of interactions (including signaling system establishment) of these microbes in plant nutrition, water uptake and imparting resistance to stresses Management of these microbes for the benefit of agriculturally important plants. Module V Definitions and nature of endophytes and how to identify endophytes Establishment, growth and transmission of endophytes Roles the endophytes play in plant metabolism, nutrition and resistance to stresses Interaction among endophytes Endophyte pathogen transitions Use of endophytes in enhancing quality and quantity of plant products (secondary metabolites and nutritional Quality) for human use Phyllospheric microbes: nature and interaction with plants. Lab/Practical Details: Isolation of Rhizobium from root nodules. In vitro Interaction of a bacterial and fungal species Rhizospehric and non rhizospheric microbial count Microbiota of phyllosphere Weightage (%) 10 15 20 25 30 General Phytochemical Screening for presence or absence of Secondary Metabolites Pedagogy for Course Delivery: (a) Lecture Plan/Session Plan : Lectures, Writing on Board, Power point presentation, discussion of various Case studies. (b) Lab Practicals: Hands-On Training (c) Self Work: Case Studies, Review of Literature, Home Assignment Assessment/ Examination Scheme: Theory L/T (%) Lab/Practical/Studio (%) End Term Examination 75 25 100 Theory Assessment (L&T): Components (Drop down) Weightage (%) Class Test 1 5 Continuous Assessment/Internal Assessment (30) Self-work Home Assignment Seminar/ Presentation 10 5 5 End Term Examination Attendance 5 70 Lab/ Practical/ Studio Assessment: Components (Drop down) Weightage (%) Continuous Assessment/Internal Assessment (40) Performance Lab record Viva Attendance 20 10 5 5 End Term Examination (60) Practical Viva 40 20 Text & References: Microbial Activity in the Rhizosphere (Soil Biology Series), Published by Springer-Verlag, Germany, Editors: KG Mukerji, C. Manoharachary & J. Singh (2006) (ISBN 9783540294207) Mycorrhiza Manual, Published by Springer-Verlag, Germany, Editor: A. Varma (ISBN: 9783540624370) Plant Pathology 5th edition (2005) GN Agrios Elsevier Academic Press (ISBN: 9780120445653) Any other Study Material: Research Papers
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