Hellenic Accreditation System S.A.
Annex F1/2 to the Certificate No.723
SCOPE of ACCREDITATION
of the
Testing Laboratory
of
CV SAFEFOOD LABORATORIES LTD
Tested materials/ products
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
Microbiological testing
Potable water
Surface water and
groundwater water
Swimming pool water and
recreational bathing water
Seawater
Water from closed hot and
cold water systems
Water from central heating
units and water from cooling
systems
Page 1 of 3
1. Enumeration of Heterotrophic Bacteria
in Water (Membrane Filtration Method)
using Yeast Extract Agar incubated at
22oC, 30oC and 37oC
In house method (WT SFL 01) based on
The Microbiology of Drinking Water
(2007), Part 7 - Methods for the
enumeration of heterotrophic bacteria.
Methods for the Examination of Waters
and Associated Materials, Environment
Agency, UK.
2. Detection and Enumeration of
Coliform Bacteria using Membrane
Lactose Glucuronide Agar (Membrane
Filtration Method) incubated at 37oC.
Confirmed using Lactose Peptone Water
In house method (WT SFL 02C) based
on The Microbiology of Drinking Water
(2009), Part 4 - Methods for the
isolation and enumeration of coliform
bacteria and Escherichia coli (including
E. coli O157:H7). Methods for the
Examination of Waters and Associated
Materials, Environment Agency, UK.
3. Detection and Enumeration of
presumptive Coliform Bacteria using
Membrane Lactose Glucuronide Agar
(Membrane Filtration Method) incubated
at 37oC.
In house method (WT SFL 02P) based
on The Microbiology of Drinking Water
(2009), Part 4 - Methods for the
isolation and enumeration of coliform
bacteria and Escherichia coli (including
E. coli O157:H7). Methods for the
Examination of Waters and Associated
Materials, Environment Agency, UK.
4. Detection and Enumeration of
Escherichia coli using Membrane
Lactose Glucuronide Agar (Membrane
Filtration Method) incubated at 37oC.
Confirmed using Lactose Peptone Water
In house method (WT SFL 03C) based
on The Microbiology of Drinking Water
(2009), Part 4 - Methods for the
isolation and enumeration of coliform
bacteria and Escherichia coli (including
Annex F1/2 to the Certificate ESYD No. 723
31.08.2011
Tested materials/ products
Potable water
Surface water and
groundwater water
Swimming pool water and
recreational bathing water
Seawater
Water from closed hot and
cold water systems
Water from central heating
units and water from cooling
systems
(continued)
Page 2 of 3
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
and Indole Production
E. coli O157:H7). Methods for the
Examination of Waters and Associated
Materials, Environment Agency, UK.
5. Detection and Enumeration of
presumptive Escherichia coli using
Membrane Lactose Glucuronide Agar
(Membrane Filtration Method) incubated
at 37oC.
In house method (WT SFL 03P) based
on The Microbiology of Drinking Water
(2009), Part 4 - Methods for the
isolation and enumeration of coliform
bacteria and Escherichia coli (including
E. coli O157:H7). Methods for the
Examination of Waters and Associated
Materials, Environment Agency, UK.
6. Detection and Enumeration of
Pseudomonas spp. in Water using
Pseudomonas C.F.C Agar (Membrane
Filtration Method). Confirmed using
Oxidase reagent.
In house method (WT SFL 04C) based
on The microbiology of drinking water
(2010), Part 8 - Methods for the
isolation and enumeration of
Aeromonas species and Pseudomonas
aeruginosa by membrane filtration.
Methods for the examination of water
and associated materials, Environment
Agency, UK.
7. Detection and Enumeration of
Pseudomonas aeruginosa in Water
(Membrane Filtration Method) using
Pseudomonas C.N Agar. Confirmed
using Milk Cetrimide Agar
In house method (WT SFL 05C) based
on The microbiology of drinking water
(2010), Part 8 - Methods for the
isolation and enumeration of
Aeromonas species and Pseudomonas
aeruginosa by membrane filtration.
Methods for the examination of water
and associated materials, Environment
Agency, UK.
8. Detection and Enumeration of
presumptive Pseudomonas aeruginosa in
Water (Membrane Filtration Method)
using Pseudomonas C.N. Agar.
In house method (WT SFL 05P) based
on The microbiology of drinking water
(2010), Part 8 - Methods for the
isolation and enumeration of
Aeromonas species and Pseudomonas
aeruginosa by membrane filtration.
Methods for the examination of water
and associated materials, Environment
Agency, UK.
9. Detection and Enumeration of
Enterococcus spp. in Water (Membrane
Filtration Method) using Slanetz and
Bartley Agar. Confirmed using Bile
Aesculin Agar
In house method (WT SFL 06C) based
on The Microbiology of Drinking Water
(2010) Part 5 - A method for the
isolation and enumeration of
Enterococci by membrane filtration.
Methods for the Examination of Waters
and Associated Materials, Environment
Agency, UK.
10. Detection and Enumeration of
presumptive Enterococcus spp. in Water
(Membrane Filtration Method) using
Slanetz and Bartley Agar.
In house method (WT SFL 06P) based
on The Microbiology of Drinking Water
(2010) Part 5 - A method for the
isolation and enumeration of
Enterococci by membrane filtration.
Methods for the Examination of Waters
Annex F1/2 to the Certificate ESYD No. 723
31.08.2011
Tested materials/ products
Potable water
Surface water and
groundwater water
Swimming pool water and
recreational bathing water
Seawater
Water from closed hot and
cold water systems
Water from central heating
units and water from cooling
systems
(continued)
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
and Associated Materials, Environment
Agency, UK.
11. Detection and Enumeration of
presumptive Staphylococcus spp.
(Membrane Filtration Method) using
Mannitol Salt Agar incubated at 37oC.
In house method (WT SFL 07P) based
on American Public Health Association
(1999). Standard Methods for the
Examination of Water and Wastewater,
20th edition, American Public Health
Association, Inc., New York, USA.
12. Detection and Enumeration of
Legionella pneumophila and Legionella
spp. in Water (Membrane Filtration
Method). Identification by serological
methods using Latex agglutination
covering Legionella pneumophila
serogroup 1, serogroups 2-14 and
Legionella spp.
In house method (WT SFL 08C) based
on BS 6068: Part 4: Section 4.12: 1998.
Detection and enumeration of
Legionella, UK.
13. Detection and Enumeration of
presumptive Legionella spp. (Membrane
Filtration Method).
In house method (WT SFL 08P) based
on BS 6068: Part 4: Section 4.12: 1998.
Detection and enumeration of
Legionella, UK.
Site of assessement: Permanent Laboratory premises, 14 Mattheou Street, Ayios Athanasios Industrial Zone,
CY-4104, Limassol, Cyprus.
Approved signatories: C. Vorkas.
This Scope of Accreditation replaces the previous one dated 20.12.2010.
The Accreditation Certificate No.723, to CYS EN ISO/IEC 17025:2005, is valid until 19.12.2014.
Athens, August 31, 2011
George K. Anastassopoulos
Chairman
Page 3 of 3
Annex F1/2 to the Certificate ESYD No. 723
31.08.2011