From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
The
Application
Stained
By
A
R.
SI1\IPLE
iron
as well
Blue
Stain
to Previously
and Bone
Marrow
SUNDBERG
METHOD
FOR
their
particulate
has made
the marrow
ill
blood
DOROTHY
aIld
rocytes
containing
method
of the Prussian
Films
of Blood
AND
HAII1IIETTE
STAINING
precursors
BIIOMAN
non-hemoglobin
and
within
irOn
macrophages
iron has been itl use in our laboratories
possible
rapid
estimates
of the amount
and in the blood
in both
new
and
old
as in both
newT and
old
section
within
eryth-
and
other
since
1951
cells
This
.
of non-hemoglobin
films
of
marrow
or
material.
LITERATURE
Gruneberg’
which
were
fixation
described
positive
for
with
mixed
absolute
solution
iron
“siderocytes”
as
when
subjected
methyl
of 1 per
alcohol,
cent
fixation
(3
parts)
with
followed
by
and 2 per
ceilt
fuehsin.
Of
basic
fact
that
they
applied
of the
then
mixed
illterest
gratlules
blue
with
type of iron
which
were
granules.
prussian
Pappenheimer
positive
did
When
siderocytes
was
recovered.
Whell
salt
solution,
found.
felt
these
by
while
the
their
study,
the
“iron
reason
Rath
in
and
cells
From
Submitted
the
the
Finch
estimating
thelial
color;
i)lackened
their
over
that
et
Giem.sa
granules
(1948)
the
total
in unstained
University
May
have
amount
films
of Minnesota
3,
1954;
accepted
their
by
They
this
that
stain
the
and
high
al2 sent
and
found
were
not
made
of
their
many
extensive
stainable
of fragments
reaction,
the
the
lIl
black
use
R.
fixed
160
July
et
side
the
Case.
Case
or free
Minlseapolis,
1954.
of a tube
course
of
applied
hut
for
some
blue
reaction
granules.
prussian
al.2
might
bodies
had
iron was oh-
ret.iculoendo-
marrow.
6,
cent
were
ouie
present
of the
Laboratories,
publication
M.
siderotic
in
per
i)odies
During
siderocytes
iron
0.9
Here
magnetized
with
of
were
Pappenheimer
bottom.
to
Feulgen
bodies
hemosideriti.
of bone
Hospital
for
in
then
granules
were
iron-cotltaining
slides
identical
the
percetltages
hydrogen
not
to
was
demonstrable
alcohol,
and
occasioulal
toward
settled
acid
counterstained
that
occasional
iron-containing
siderocytes
actually
contained
migration
alcohol
of siderocytes
with
the
oiily
were
methyl
hydrochloric
cent
showed
cases
sulphuretted
erythrocytes
stain”
Gram
with
bodies
Pappenheimer
also
the
treated
a brown
scarlet.
used
structures
with
acid
(1945)2
of
is
demonstrating
felt
from
it is of some interest
Further
proof that
unaffected
et al.
take
a freshly
hydrochloric
that
the stippled
erythrocytes
erythroeytes
cotitaitiing
some
numbers
iron-containing
comment
that
blackened.
not
with
cent
biebrich
study
showed
actually
large
on
hemosideriui
the
been
tamed
w-ere
took
in their
They
w’ere
blood
sedimented,
most
Since
that
reagent.
Giemsa
heparinized
1 per
of 1 per
(1 part).
and rod-like
decolorized
stain,
prussian
stained
treated
and
(1945)2
ferrocyanide
particular
containillg
granules
blue reaction.
After
were
with
Smith
solution
potassium
the
blood
negative.
films
ferrocyanide
counterstained
Parker
and
a freshly
photographed
with
the Giemsa
erythrocytes
blood
potassium
acid for 3 to 5 minutes
and
Pappenheimer,
Thompson,
erythrocytes
to the prussian
Minn.
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It.
Mills,
Huff,
Krupp
erythrocyt.es
and
coutlter-stained
Despite
the early
placed
out the practice
could
t.hetl
taitling
apply
agaiil,
they
used
formaliui
vapor
solution
of
2 per
chloric
a(i(i.
After
for 30 to 60 seconds
the
show-ed
that
May-Grunwald
in tap
Their
basic
water,
reaction
emphasis
was
iron as a type
decolorize
maryears before
and
plasma
Giemsa
cells
and
conthen
6
‘
(1954)
.
stippling
blue
could
Giemsa
He illustrated
May-Grunwald
blue.’
and
similar
prussian
one
have
described
method
prior
t.o staining
for one
potassium
ferrocyanide
a brief wash
with dilute
in rubricytes
showed
et al. (1945)2
no real
designed
to demousstrate
Turuihull
30 minutes
cent
aqueous
for
also
to
blue
reaction.
first with
the
with
stipplitig
and
sui)jected
(1952)
with
Turnbull
stained
161
BROMAN
illustrated
Zuelzer,
and Mouriqualld
on fresh
films of marrow
Kaplan,
which
Koszewski
heeul stained
the
which
HARRIETTE
stain
erythrocytes
decolorization,
after
Recetltly
(1950)
with safranine.
report
of Pappenheimer
of using a reaction
uuitil
had
graulules
Garcia
AND
Wright-Giemsa
rubricytes
and
of counterst.ain
row films which
SUNDBERG
and
the
with
different
in
DOROTHY
their
a method
involved
hour
and
fixation
in equal
2 per
slides
w’ere
blood,
bone
in
parts
of a
cent
hydro-
counterstaiuled
fuchsin.
rFECHNIC
Dry
films
of
a
large
variety
sputuni
ttn(l ascitic
fluid
or
be staitled
for tsoishemoglobin
1. The
filni
or iml)rint
2.
slide
is flooded
state
The
for
4.
The
stain
to
The
niixed
are
left
stained
in
of
reaction
allowed
and
the
stain
the
and
of various
stain
blue
and
are
should
marrow,
tissues
lymph,
or
remaits
organs
can
in
this
undiluted
iron
in
is
original
is
films
recognizable
also
a vivid
blue
of
freshly
(1 part).
briefly
stained
easily
nongranular
up
in
distilled
dry.
and
still
made
of time
ferrocyanidet
green,
The
a Period
washed
drain
little.
concerned,
is changed
brought
to
distinguish
cells resembling
biopsies
of the
are
which
10 minutes4
blue
for
with
there
Wright’s
has
been
and
cytoplasm
or
blue
of
green,
and
stain,
a minimal
the
original
many
of
the
fragments
of
color.
‘s stain
wts
to
cells
very
or
slide
film.
potassium
to
to the
the
the
reagent
cent
allowed
blue
is often
similar
for
on
of
2 per
reagent
then
a vivid
remain
blue
and
comparable
altered
to
cellularity
prussian
Individual
particulate
paper
iron
way
been
It
Wrigist
att.em)tiulg
prussian
macrophages
as
the
(3 parts)
is colored
Isas
are
with
in
appears
decolorization.
cytoplasm
Iiisoftr
the
in this
hemosiderirs-filled
ticulate
and
stain
acid
color
iron
staining
* This
buffer
the
immersed
a 1)iflk
amouist
purple
with
lsydrochloric
particulate
this
including
rapidly.
\Vright’s
of
are
cent
urstil
Slides
is dried
strength
slides
slides
but
fluids
or touch
preparations
in the following
manner.
with
is diluted
the
1 l)C
water
cellular
4 minutes.
3. The
suitable
of
imprint
iron
our
to
that
an
attention
by
various
types
between
many
we
had
seen.
marrow,
it
was
desirable
Because
to
which
even
Dr.
many
have
otsce
more
vivid
Rudi
of
appeared
blue
Schmid
stippling.
of
a method
the
cases
for
deep
or
blue
at
a time
The
when
had
t.he
or
color
illustrations
studied
proving
blue
green
even
than
we
were
showed
had
presence
previous
of
par-
01(1 specimens.
0.5 per cent and 4
cent
potassium
ferrocyanide
have
also been
used
with
apparently
equal success.
The
proportions
and strengths
used
were
t.hose
used
by Pappenheimer
et a!.2
They
were recommended
by Dr. Roy Holly who had consulted
Dr. Clement
Finch
regarding
the best stain.
This reaction
is reasonably
clear at 2 minutes,
but the granules
seem less discrete
than
at 5 or 10 minutes.
At 30 minutes
(the time recommended
for tissues),
decolorization
is still
not too great,
hut
nuclei
show some fading,
and Jolly bodies
may be very difficult
to see.
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
162
PRUSSIAN
that
obtained
green
with
with
tentional
colorizes
and
changes
as
long
the
equal
as 30 years
prussian
of this
inaiss-
atsd
on
blue
originally
films
it
with
Wright’s
a type
bluein-
reagent
make
the
of
counterstain,
Wright’s
stain
or
No
blue
to
as
with
blue
originally.
prussian
sufficiently
reagent
stained
stained
appeared
showed
The
.
iron
prussiali
films
nsethod
have
macrophages
as
1. Methyl
alcohol
The
well
as
included
laden
many
fixation
outlined
safranine
or
various
studies.
with phagocytosed
siderotic
or the
de-
iron
easily
has
Gienlsa
immediately
stains
prior
Comparable
erythrocytes
isormoblasts
to
using
and
films of the same
and particulate
siderocytes
have
been
sub-
these
similar
and
cytoplasm
siderotic
blue
followed
three
and
types
prussian
by
to
blue
(10
reagent
Wright’s
or
the
minutes).
Giemsa
stains
or
by
Wright’s
the
granules
precursors,
stain
follow-ed
the
by
the
in w-hich
numbers
types
prussiatl
HO
of cells
are
blue
granules
of specimens.
percentages
in the
of specimetl
cell
macrophages
positive
types
in
in large
iron-laden
prussian
of the
similar
type
visible
but
of
of the
in each
are
first
are
numbers
color
siderocytes
In
siderotic
reaction.
the
blue-green
is similar
and
and
blue
of preparations
blue
of specimens.
the
the
followed
Prussian
of macrophages
erythrocytes
The
the
by
types
the
normoblasts
used,
and
reaction
fuchsin.
stain
are
(4 minutes)
prussian
basic
3. Wright’s
two
on
which
than
is necessary
particulate
the
that
color
to:
2.
is
success
(hemosiderin?)
In
of the
and
intense
reagetst.
having
jected
color
STAIN
alone,
a more
decolorization
using
ago*
blue
Controls
marrow
iron
the
reaction
on
of
method,
with
blue
takes
l)hase
Tisis
used
prussian
stain
separate
identifiable.
been
the
Wright’s
BLUE
not
count-erstain
i)elieved
clearly
reagent
The
latter
to be
recoguiizable.
affords
the
or which
type
best
cy-
tology.
Efforts
pling
to predict
ill
which
and
erythrocytes
of the granules
iii macrophages
normoblasts
will be prussian
been uniformly
successful.
Much
of the
phages
or usormoblasts
and eryt.hrocyt.es
with
green
the
prussian
and
olive
blue-green,
normoblasts
have
blue
green
i)eett
and reidentified.
Some,
phages
or normoblast.s
olive-green
pigmetit-
disappears.
which
coarser,
plasma
as well
iFOtl
cells
blue-green
most
occasionally
1)een
as
rocytes
and
reticulocyt.es
brilliant
cresyl
also
* Blood
or stained
w’hen
film
from
greeu
pernicious
or
1)lue-green
found
which
studied.
blue
or
proves
blue
erythrocytes
prussian
blue-green
reagent
ill
positive.
Some
and
blue
pigmetlt
prussian
disappears.
stippling
in neutrophils,
and
of neutrophils
disappeared
blue
to the
are
Some
of
in erythrocytes
the
brilliant
anemia
files
of the
stipplitlg
stippling
will show
reaction.
monocytes,
and
precursors.
The
spongioplasm
stippling
the prussian
of ret.iculocytes
cresyl
of 1)r.
and
basophilic
hasophilic
blue
to t.he prussian
from
prussian
macro-
negative.
prussian
disappeared
with
The
reticulation
with
the
lymphocytes,
the precipitated
or the ordinary
subjected
after
not
macroor blue-
often
it is the fine blue-green
basophilic
the cell which
contained
that
stippling
that it was
cytoplasm)
normoblasts
were
also
have
containing
stippled
subjected
of the
iui macrophages
D#{246}hlebodies
Because
it seemed
nucleic
acid bearing
olive
brilliant
has
all
not
macrophages
numerous
erythrocytes
remains
Although
disappears,
a somewhat
Particulate
stain
hut
auld
Numerous
and
sketched,
circled,
of stip-
positive
deep
l)lue granulation
in either
will become
a brilliant
blue
reagent.
pigments
blue
Hal
plus
reagent
Downey.
(riboof eryth-
reagent,
films of
staiuled
with
a Wright’s
for
counter-
10 minutes.
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
R.
In the course
from
a patient
types
of
unstained
noma
cells.
golden
Wheul
phages
very
were
cells
found
of
the
all pigment
itt
the
The
contain
the
the
(riot
of these
distinguishable
and
still
reagent
granules
a very
of liver
both
macrophages
pigmetit
prussian
blue-greetl
films
the
used
could
be
as
melanot
was
of marrow
or imprints
found.
which
resembles
of liver,
dis-
a counterWith
few’ hemosideriul-containing
marrow
reagent
tissue
2.
water.
Stain
for
3.
4.
Wash
in tap
Counterstain
5.
Do
not
stain,
golden
brow-Il
pigment
reagent.
These
studies
of course,
1. Take
Wright’s
pigmeilt
of the
the
prussian
blue
and,
tilled
brown
imprints
In
the
macro-
although
numer-
had
heetl
identified
prior
to
afforded
further
evidence
that
some
hemoglobin
i)reakdow-n
product
Oil
of
of
iron.
prussian
marrow
to
only
163
BROMAN
of bone
marrow
and
w-ere also studied.
in many
bright
alone,
in the
containing
not
HAIIIIIETTE
seen
With
occasional
reaction
application
does
with
hemosiderin.
prussian
ous
was
stained
from
Oully
AND
yellow*
hemosiderin)
tinguishable
simple
SUNDBERG
of this investigation,
films
with
malignant
melanoma
preparations
from
stain,
DOROTHY
has
also
been
tissues.
through
xylol
and
the
prussian
30 minutes
with
water
w’ith
Here
for 20 minutes.
hematoxylin
for
decolorize
the
used
sections
particles
the technic
is as follows:
decreasing
strengths
of alcohol
other
boule
to
dis-
reagent.
2 minutes
and
eosin
for
a few
secoulds.
hematoxylin.
6. Dehydrate.
Coverslip
With
this technic
bright
macrophages
and phagocytic
from xylol.
blue-green
granules
of varyiusg
sizes are visible
in
cells of all types
atld also ill nornoblasts
and eryth-
rocytes.
extremely
of prussian
is, how-ever,
Some
of the masses
large ; their
color
(
and
section
siderocytes)
If new’
old
slides,
and
the
the
material
tissue
prussiatl
is not
passed
reagent
positive
similar
available,
through
applied.
material
to that
the
Restaining
of
cell
various
with
the
films
Zuelzer,
and
and
marrow
the
particulate
centage,
of marrow’
objective
* The
here,
It
.
The
iron
remarkable
of
pigment.
Finds3
aIs(!
to
alid
refract
orv
aiiemuas.
t Isat
is
vivid
counts,
very
seen
similar
in
color
anemias,
to
By
described
The
blood
films
that
using
that
by
Kaplan,
these
as
osis,
blood
and
siderocyte
per-
from
two
atid
a higher
described
hemocisromat
obtaitled
on previously
fixed and stained
fairly
well maintained,
on
determined
uistained
those
hemochromatosis
atlemia).
was
to
color.
estimated
ott
not
through
identification
method
method
generally
(exogenous
it
us hemolytic
the
blue-greeti
was
megaloblastic
comparisous,
seemed
and
stain
similar
to previously
water,
is getierally
used
their
are
from
to
passage
reasouiahle
seem
to use
that
siderocytoses
splenectomized
means
found
expected
cell
an additional
results
be applied
original
is the
1000
Oil
hematoxylin
and
atid
be removed
of alcohol
method7
we decided
w’as
can
their
can
w-ith
of normoblasts
because
Mouriquand
based
tomized
and
described
films.
with
estimation
types,
method
stained
tients
allowed
macrophages
the erythrocytes
itl
strengths
but the original
eosin usually
fades,
eosill
is desirable.
Because
Kaplaul,
Zuelzer,
and
Mouriquatld’s
of marrow’
coverslips
decreasing
necessary,
films
the
itt
seen
pa-
gastrec-
counts
as
au
percentage
hemosiderits
megaloblast
of
by
Rath
Ic ausemias
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
164
1 . -I’atient
TABLE
with
Fe, P10 B
RDS
F
9.6
PRUSSIAN
BLUE
Exogenous
Hemochromatosis-Siderocytes
P60 B
STAIN
in
KZM
13.5
10.1
Blood
wP60
\V Fso P60
6.4
9.6
7.0
LMG
Gastrectomized
Splenectom
F
ized
P10 B
Patient
with
Fio P10 B
KZM
W F
p10
Blood
\V Fo
r
C’
%
%
%
%
%
40.9
39.7
37.3
19.6
38.6
37.1
40.2
HB
37.5
35.5
34.9
22.7
39.9
36.7
34.9
39.3
35.1
38.5
18.8
39.0
36.8
34.6
38.0
F30-Formol
gent-concentration
same
as
lined
above-60
minutes.
W-Wright
.
minutes.
siderocytes
was
in
F10-Formol
vapor-lO
minutes.
P,o-Prussian
reapreseist
report-lO
minutes.
P60-Prussian
reagent-
B-Basic
‘s st am
.
fouuid
w’hen
fuchsin.
K-Prussian
presetlt
staiil
by
immersion
formalin
of
fLxat.iOIl
ion
yeas
used
Kaplan
as
et
al.7
described
either
as
out-
Kaplan,
by
originally
or
after
with
Wright’s
stain
thaul when
the only
seemed
to be the only variable
responsible
and fixation
with formalin
for 10 minutes
prussian
reagent
(concentration
as
described
in
report
or as recommended
by Kaplan
et al.7) for 10 minutes
provided
with as great. a degree
of “positivity”
as that obtaitled
with 30 minutes
and
fixation
positivity
(olor
the
in
KZM-Method
reagent-concent.rat
the methyl
alcohol
fixatioul
employed
fixative
was methyl
alcohol.
Formalin
for a higher
per(entage
of siderocytes
followed
K
21.6
vapor-30
minutes.
as described
al.7-60
60
minutes
provided
by
reaction
counts
Counts
of
couuits
as well
were
to
fixation
and,
iti table
1 give
variability
by three
the
prussian
seems
particles
as the
done
exposure
formaliti
of mitiute
staiuial)le
iron.
The siderocyte
cell
W Fo P,o
WP10
in
RDS
LMG
et
4 nemia-Siderocytes
Megalobiastic
to
sometimes
an
idea
apparetitly
of us (R.
reagent.
be
based
delicate
of the
H. B.,
increased
the
fuzzy
blue-green
aggregates
variability
induced
D. S.,
The
upon
of even
by formalin
and
L. M.
a
of
of
1000
fixation.
G.*)
DIsCusSION
the
The technic
siderotic
(rihonucleic
phages
prussian
described
has proved
granules
of siderocytes
acid)
which
blue
aIld
resemi)les
reagent.
.Jolly
bodies.
valuable
in the
and normoblasts
It has
hemosiderin
does
Because,
with
an
prussian
blue reagent
can he applied
after
utes)
have
already
been
stained
and still
* Lorraine
Hospitals.
M. Gonyea,
MS.,
Instructor
in
also
shown
problem
of differentiating
from
basophilic
stippling
that
not contain
iron
added
10 minute
all pigment
in macro-
which
stains
with
staining
period,
films,
imprints,
or sections
provide
a clear-cut
method
Medical
Technology,
University
the
the
(30 mmof dis-
of Minnesota
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
R.
tinguishitlg
atld new
useful
particulate
specimens
in the
made
smears
DOROTHY
SUNDBERG
iron, the
which
may
study
from
of iron
marrow
method
contain
165
BROMAN
HARRIETTE
is invaluable
in the
excesses
of particulate
deficiency
particles
desirable
since often
virtually
made
from the myeloid-erythroid
the
AND
comparison
iron.
It
anemia,
but in the latter,
as recommended
by Rath
no particulate
or huffy
iron can
coat layer
the
staining
and
positive
to purple
reagent,
which
show’
with Wright’s
stain,
but
all assume
a reasonably
which,
similar
studies
suggest
siderin,
probably
material
if the
that
hemosiderin.
the
The
diffuse
could
be due to the more
The method
described
Kaplan,
Zuelzer
gree of positivity
to allow
blue
granules
can
a feature
tiot
in
and
the
or fuzzy
by
and
ferritin.
with
that
prussian
blue
The
present
on previously
stained
by these
investigators.
as hemo-
of
are
also
macrophages
recently
presented
by
shows
a variably
higher
dethe ability
of formalin
vapor
particles
Kaplan
masses
to blue,
siderocytes
cytoplasm
Their
method
to be based
upon
described
be used
pointed
out
counterstam,
color
and
blue-green
of the
color.
is
films
studied,
is to he regarded
normoblasts
soluble
form of iron,
has been
compared
method
from
with the additioul
vivid
blue-green
in
blue-green
of minute
The
varying
macrophages
in
granules
and Mouriquand.7
which
appears
staining
reagent.
material
siderotic
colors
of
Finch3
be foutld
in ordinary
of cetitrifuged
marrow.
Bone
marrowand blood
from
a variety
of conditiouls
has been
results
are the subject
of a separate
report.8
The term
“particulate
iron”
is used here to refer to the granules
of prussian
of old
is also
of iron
with
the
prussian
et al.7 minus
the
basic
fuchsin
dry
films
of blood,
marrow-,
etc.,
SUMMARY
A simple
blasts,
films
method
for
macrophages,
of cellular
and
fluids
staining
other
or imprints
non-hemoglobin
cells
iron
containing
of tissues
itl
erythrocytes,
particulate
and
organs
iron
normoin
is presented.
new
or
This
old
method
ill using
the prussian
blue reagent
as a type of counterstain;
no separate
decolorization
is necessary.
The
preparations
obtained
resemble
the original
preparations
except
that iron stands
out as a vivid
blue-green
material.
The
method
is particularly
useful
in studying
conditions
accompanied
by
varyitlg
degrees
of iron
excess
or hemosiderosis
of the marrow’.
consists
The
stainable
of the
cytoplasm
of iron,
ferritin.
Stainable
iron is all virtually
of macrophages
iron
is visible
phages
in sections
A prussian
blue
subjected
method
the
might
in normoblasts
and
to the prussian
using
formalin
blue
fixation
also i)een shown
to
viously
stained
films
be useful
in the
of marrow
and
bring
percentage
about
a higher
Es
presetitate
un
normoblastos,
ferro-si
hen
simple
ill flO\’C
methodo
diffuse
to the
erythrocytes
blue-green
soluble
more
as well
react-ion.
on fresh
demonstration
blood.
The
films
color
form
as in macro-
of marrow-7
of particulate
formalin
fixation
iron iii
appears
has
preto
IN IXTERLLNGUA
pro
macrophagos,
e preservate
The
of siderocytes.
SUMMARIO
rocytos,
same
color.
be attributed
colorar
e altere
frotis
de fluido
ferro
iui eryth-
non-hemoglohinic
cellulas
cellular
a
particulas
como
etiam
coust.ineuste
in impres-
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
166
PRUSSIAN
siones
de
Prussia
texitos
como
colorante
es
except.e
Le
requirite.
Clue ferro
Omne
le ferro
diffuse
methodo
Ferro
phagos
colorate
i)lu-verde
in
Le met.hodo
utile
in studiar
o de hemosiderosis
le methodo
appare
usar
ill
le
a ferritina
es visibile
dine
in
itltraparticular
reagente
blu
de
es un
pare
le mesme
de macrophagos
forma
plus
solubile
nuance.
es possibile-
de
ferro.
e etiam
e erythrocyt.os
a blu
de
accompaniate
medulla.
pract.icameiit.e
normoblastos
in
macro-
de Prussia.
con fixation
a formalina
que Kaplan,
Zuelzer,
frotis
de medulla
es etiam
utile in le demonstra-
in previemente
a formalina
del
exhibi
al reactioul
subjicite
conditiones
le cytoplasma
in
a blu de Prussia
ha usate
pro nove
de ferro
Le fixation
per
que
sectiones
Mouriquand
consiste
de cont.ra-coloratlte.
Nulle
processo
specificamente
deLe preparatos
obteuiite
es simile
al preparatos
original
se distingue
como
materia
de color vivement-e
blu-verde.
at.tribuibile
colorabile
mente
tiOtl
Iste
STAIN
specie
met.hodo
es specialmente
grados
de excesso
de ferro
vane
Le
e organos.
Un
BLUE
distitictiuicar
colorate
uti
de medulla
frotis
plus
alte
procentage
e
e sanguine.
de siderocytos.
REFERENCES
1
H.
GRIJNEBERG,
A. M.,
2 PAPPENHEIMER,
associated
14:
75,
RATH,
: Siderocytes:
with
A new
unidentified
kind
of erythrocytes.
\V. P.,
THOMPSON,
PARKER,
erythrocytic
Nature,
D.
inclusions
P.,
aft.er
London
148:
K.
SMITH,
AND
splenectomy.
114,
1941.
E. : Anaemia
J. Med.
Quart.
1945
C. E.
AND
C. A.:
FINCH,
Sternal
marrow
hemosiderin.
A method
for
the
determina-
in man. J. Lab. & Clin. Med. 33: 81, 1948.
MILLS,
H., HUFF,
R. L., KRUPP,
M. A., AND GARCIA,
J. F.: Hemolytic
anemia
secondary
to a familial (herditary)
defect
in hemoglobin
synthesis.
Arch.
Int. Med. 86: 711, 1950.
‘ItoSZEWSKI,
B. J.: Zur Methodik
des H#{228}mosiderinnachweises
in den Blut- und Knochenmarksausstrichen.
Klin. Wchnschr.
30: 926, 1952.
6 -:
The
occurrence
of megaloblastic
erythropoiesis
in patients
with hemochromatosis.
tion
of available
Blood
KAPLAN,
7:
1182,
E.,
iron
1952.
GONYEA,
lrecursors
W. W.,
ZUELZER,
nonhemoglobin
8
stores
iron
L. M.,
AND
in the
in marrow
AND
It.
SUNDBERG,
blood
and
bone
C.:
MOURIQUAND,
isormoblasts.
D.:
marrow.
Blood
A study
(To
Sideroblasts.
9:
203,
of stainable
be published.)
A study
of
stainable
1954.
iron
in erythrocytes
and
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1955 10: 160-166
The Application of the Prussian Blue Stain to Previously Stained Films of
Blood and Bone Marrow
R. DOROTHY SUNDBERG and HARRIETTE BROMAN
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