Transendothelial migration leads to protection from
starvation-induced apoptosis in CD34+CD14+circulating
precursors: evidence for PECAM-1 involvement through
Akt/PKB activation
by Elisabetta Ferrero, Daniela Belloni, Paola Contini, Chiara Foglieni, Maria Elena
Ferrero, Monica Fabbri, Alessandro Poggi, and Maria Raffaella Zocchi
Blood
Volume 101(1):186-193
January 1, 2003
©2003 by American Society of Hematology
Survival of transendothelial migrated and nonmigrated cells without growth factors.PBMCs were
allowed to transmigrate overnight across HUVEC monolayers.
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Kinetics of starvation-induced apoptosis after transendothelial migration.Peripheral blood
adherent mononuclear cells were allowed to transmigrate overnight across HUVEC monolayers.
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Viability of cells migrated through endothelium.Peripheral blood CD14+ cells were allowed to
migrate across HUVEC monolayers.
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Increased mitochondrial ATP and NAD/NADH ratio in migrated cells.ATP content (A,C) and
NAD/NADH ratio (B) were measured in migrated (▪) and nonmigrated (■) cells.
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Role of PECAM-1 homophilic interaction in inducing survival of CD34+ cells.CD31+CD34+ (■) or
CD31−CD34+ (░) cells were allowed to adhere to PECAM-1+–transfected ECV304 (P-ECV304; A)
or to the mock-transfected ECV304 cell line (B), in the absence or presence...
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Regulation of Bcl-2, Bcl-X, A1, and Bax in CD34+ cells upon PECAM-1 interactions.(A) Cells were
allowed to adhere to PECAM-1+ transfectants (D12P, lane 3) or to mock-transfected NIH3T3
fibroblasts (mock, lane 2) for 30 minutes, cultured for 36 hours, lysed,...
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
Engagement of PECAM-1 leads to Akt activation.(A) Akt activation evaluated by Western blot
with mAbs recognizing unphosphorylated (Akt; bottom) or phosphorylated Akt (P-Akt; top) in
purified CD14+CD34+ cells after 2 minutes (lanes 2 and 4) or 4 minutes (lan...
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology
PECAM-1–induced survival of CD34+ cells is dependent on PI-3K activation.CD14+CD34+ cells
were allowed to adhere to PECAM-1+–transfected fibroblats (D12P; A) or to mock-transfected
cells (B), in the absence or presence of the F(ab′)2 of the anti–PECAM-1 ant...
Elisabetta Ferrero et al. Blood 2003;101:186-193
©2003 by American Society of Hematology