Hoagland Nutrient Solution
KHO3
Ca(NO3)2.
4 H2O
(NH4)2PO4
MgSO4.
7 H2O
Compound 2
KCl
H3BO3
MnSO4
.H2O
ZnSO4.
7H2O
CuSO4 .
5H2O
H2MoO4
(85%
MoO3)
Fe-EDTA3
Conc. of
stock
solution
g / liter
101.10
236.16
1.00
1.00
101.10
236.16
Volume of
stock
solution pre
liter of final
solution (ml)
6.0
4.0
115.08
246.49
1.00
1.00
115.08
246.49
2.0
1.0
74.55
61.84
169.01
(mM)
50
25
2.0
3.728
1.516
0.338
289.55
2
0.575
Final conc.
of element
Final conc.
of element
ppm
N
N
16000
6000
224
235
Ca
P
4000
2000
160
62
Cl
B
Mn
50
25
2.0
1.77
0.27
0.11
Zn
2
0.131
Element
Conc. of
stock
solution
(M)
Molecular
weight
Compound
1
1 Liter
249.71
0.5
0.125
Cu
0.3
0.032
161.97
0.5
0.081
Mo
0.5
0.05
346.08
20
6.922
Fe
20
1.12
1 Liter
1. After Johnson et. al. (1957), modified.
2. A combined stock solution is made up containing all micronutrients except iron.
3. Ferrous dihydrogen ethyleamine tetra acetic acie.
For M.Sc Botany 3rd semester; BS Botany 7th semester
Growth responses of maize, sunflower and cotton varying nitrogen regimes
Modified Hoagland’s nutrient solution for varying nitrogen regimes 4, 8, 12, 16 and 20mM N
Sr.No
KNO3
Ca(NO3)2.4H2O
NH4 H2PO4
MgSO4
K2HPO4
CaCl2.2H2O
Fe-EDTA
Micronutrients
4
1.5mL
1 mL L-1
0.5mL
1mL L-1
2.25 mL L-1
3mL L-1
1mL L-1
1mL L-1
Varying N (mol m-3) in Hoagland’s nutrient solution
8
12
16
3mL L-1
4.5mL L-1
6 mL L-1
2 mL L-1
3 mL L-1
4 mL L-1
-1
-1
1 mL L
1.5mL L
2mL L-1
-1
-1
1 mL L
1 mL L
1 mL L-1
-1
-1
1.5 mL L
0.75 mL L
1.5 mL L-1
0.75 mL L-1
1mL L-1
1mL L-1
1mL L-1
-1
-1
1mL L
1mL L
1mL L-1
20
6.5 mL L-1
5 mL L-1
2.5 mL L-1
1 mL L-1
1mL L-1
1mL L-1
Mean values for …………………….. of maize/sunflower/cotton when 21 day-old plants were subjected
for varying concentrations of nitrogen in sand culture.
Sr No
Desi Bhindi
-3
Varying N (mol m ) in Hoagland’s nutrient solution
Rep.No
1
2
3
4
5
Mean
4
8
12
16
20
Std. Dev.
S.E
Analysis of variance of data for …………………….. of maize/sunflower/cotton when 21 day-old plants
were subjected for varying concentrations of nitrogen in sand culture.
SOV
df
SOS
MS
F value
P Value
Nitrogen
Error/Residual
Total
Cultivars
LSD =
4mM N
8 mM N
12 mM N
16 mM N
20 mM N
Effect of varying levels of N on growth of maize, sunflower, cotton
Use following treatments
T1 = N 56 mg L-1 = 1.5mL 1M KNO3 L-1 + 1mL 1M Ca (NO3)2 .2H2O + 0.5mL 1M NH4H2PO4 L-1 + 2.25
mL 1M K2HPO4 + 3mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1mL FeEDTA
T2 = N 112 mg L-1 = 3mL 1M KNO3 L-1 + 2mL 1M Ca (NO3)2 .2H2O + 1mL 1M NH4H2PO4 L-1 + 1.5 mL
1M K2HPO4 + 1.5mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1mL FeEDTA
T3 = N 168 mg L-1 = 4.5mL 1M KNO3 L-1 + 3mL 1M Ca (NO3)2 .2H2O + 1.5mL 1M NH4H2PO4 L-1
+0.75mL 1M K2HPO4 + 0.75mL 1M CaCl2.2H2O + 1mL 1M Mg SO4 + 1mL Micronutrients + 1
mL Fe-EDTA
T4 = N 224 mg L-1 = 6 mL 1M KNO3 L-1 + 4 mL 1M Ca (NO3)2 .2H2O + 2 mL 1M NH4H2PO4 L-1 + 1mL
1M Mg SO4 + 1mL Micronutrients + 1 mL Fe-EDTA