709 CONAS ENVIRONMENTAL AND METHANE POTENTIAL GAINS FROM VEGETABLE AND FRUIT WASTES The Case of Dabaga Canning Industry, Iringa, Tanzania Elizabeth Benjamin Kiunsi Master of Science (Environmental Science), October 2008 The anaerobic digestion of the peels of fresh banana, ripe banana, passion fruit, oranges and pineapples and the waste of vegetable, tomatoes and mixed waste were studied in batch cultures using normal anaerobic sludge. The various waste materials were examined for digestibility under mesophilic conditions. 264 hours of incubation time, methane production, electricity generated, Volatile Fatty Acids, pH, and buffering capacity were determined. The methane production of the studied waste showed that the highest value was obtained from tomato waste and the lowest value from mixed waste. Electricity generated ranged between 0.277.8 and 0.51.7 Mwh. The Volatile Fatty Acids ranged between 6081 and 852 mg/l, pH ranged between 7.4 and 5.31, and total alkalinity ranged between 393 and 5728 mg/l CaCO3. The highest moisture content was 31.4 % from orange peels and the lowest was 5.6 % from tomato waste. The highest ash content was 43.15 % from orange peels and the lowest ash content was 5.3 % from tomato waste. The highest Total Solid was 31.4 % from orange peels and the lowest was 5.5 % from tomato waste. The highest Volatile Solids was 94.06 % from orange peels and the lowest was 82.35 % from ripe banana peels. Methane production of the studied wastes increased as incubation time increased and after a certain maximum it decreased as incubation time increased. The amount of methane produced for all the studied wastes started with 0.0 ml, to the maximum 34.6 ml, from 0.4 ml for passion peels to a maximum of 22.4 ml, from 0.0 ml for ripe banana peels to a maximum of 33 ml, from 0.1 ml for vegetable waste to a maximum of 30.5 ml, from 0.0 ml for fresh banana peels to a maximum of 30.8 ml, from 1.8 ml for orange peels to a maximum of 7.6 ml, from 0.0 ml for Pineapples to the maximum of 39.2 ml, from 0.4 ml to a maximum of 3.7 ml for mixed waste. The results of these studied wastes showed that tomato wastes produced relatively high methane production (143.5ml), while pineapple produced relatively small amount of methane (26.4ml). However, there was relatively low methane production from mixed wastes (21.2ml) and it was concluded that buffering capacity and pH should be controlled so as to produce more methane. ************** 710 University of Dar es Salaam IMPACTS OF CLIMATE CHANGE, VARIABILITY AND ADAPTATION STRATEGIES ON AGRICULTURE IN SEMI ARID AREAS OF TANZANIA The Case of Manyoni District in Singida Region Mary Abihud Lema Master of Science (Natural Resource Assessment and Management), November 2008 Climate change is among key challenges affecting agriculture in Sub Saharan Africa particularly in semi arid areas. Impacts of climate change and variability (CC&V) and associated adaptations on agriculture were studied in semi arid area of Tanzania. The overall objective of the study was to examine the impacts of CC & V on agriculture and to establish how adaptation and coping strategies could be enhanced. The study was carried out in two villages of Kamenyanga and Kintinku of Manyoni District that represents different semi-arid and socioeconomic characteristics. Secondary data were conducted through literature review. Primary data were collected using PRA methods including; key informant interviews, focus group discussions, household questionnaires and observation. In each village, a sample size of 10 percent of all households was interviewed of which 42 households in Kamenyanga and 41 in Kintinku. Results indicated decreasing rainfall trends and changes in pattern in between 1922 and 2007 while mean maximum and minimum temperature increased by 1.9 oC by 0.2 oC respectively and average annual temperature increased by 0.7 oC in between 1984 and 2004. Local people also perceived changes in rainfall and temperature. The changes have affected crops and livestock in a number of ways resulting in reduced productivity. The study also demonstrated that variations in social groups reflect different levels of vulnerability to impacts of CC & V. The study concluded that, the wealth of knowledge on coping and adaption that farmer has should form a foundation for designing agricultural innovation systems to deal with impacts of CC & V. The study recommended to agricultural extension officer to organize trainings for farmers so as to strengthening and develop their knowledge on climate change and adaptation. ************** IMPACT OF PARTICIPATORY FOREST MANAGEMENT (PFM) ON POVERTY REDUCTION AND FOREST GOVERNANCE The Case of Suledo Forest Reserve, Kiteto District Manyara Region Alexander Songoro Master of Science (Natural Resource Assessment and Management), November 2007 This study examines the impact of PFM on poverty reduction and forest governance. Specific objectives included the assessment of the extent to which forest adjacent communities depend on the forest for livelihood; and investigating the impact of PFM on poverty reduction; and examining if the PFM has 711 CONAS improved forest governance at local level. The study was carried out in Suledo Forest Reserve in Kiteto District Manyara region. Data collection was done through questionnaire interviews and a checklist, discussion with key informants, direct observation, and documentary review. The results indicated that about 96 % of respondents were aware of PFM; and about 95 % of them noted that, before the start of PFM, the forest was used without any proper planning, that led to degradation. About 61 % of the respondents reported that their income was negatively affected by the introduction of PFM. However, improvement is seen on non-income poverty including water sources, communication, gender equity and improved environment in general. Moreover, this research noted weaknesses on governance, especially on benefit-sharing mechanisms, whereby about 96 % of the respondents had no information about how revenue from SFR is utilized and handled. Following the above findings it is recommended that, PFM should go hand in hand with improvement of agriculture and other income generating activities. Funds obtained from timber harvesting must be handled properly. There is a need also to mobilize funds and other resources to support PFM. People should be made aware of the importance of PFM on poverty reduction. ************** WETLANDS RESOURCES AND THEIR CONTRIBUTIONS TO LOCAL LIVELIHOODS IN THE RUFIJI BASIN, RUFIJI DISTRICT Hamudu Idd Hamisi Masters of Science (Natural Resources Assessment and Management), November 2007 Knowledge on the contribution of wetlands resources to the livelihoods of the rural people is insufficient in Rufiji wetlands. In order to improve this knowledge, the study was carried out in Rufiji wetlands in the villages of Mbunju-Mvuleni and Mtanza-Msona of Mkongo and Mwaseni wards respectively to understand the significance of the Rufiji wetlands resources to the livelihoods of the local communities and their implication on the integrity of the wetland ecosystems. Data were collected through PRA, focus group discussions, key informant interviews and participatory transect walks and supplemented by detailed household questionnaires administered to 112 randomly selected households in the two villages. Data were analysed using SPSS, MS-excel and Instart. Results revealed that wetland resources have significant contribution to the livelihoods of the local communities adjacent to the wetlands. Wetland agriculture and fishing are the main livelihood activities for more than 96% of the wetland adjacent communities. Harvests from wetland farming, worth more than 1500 USD are accrued per household each year whereas non-wetland farming provides less than 400 USD only a year per household. Fisheries from the wetlands provide more than 94% of the household protein requirements. Wetland resources contribute about 65% of the direct household annual income amounting to about 1700 USD million per household each year. About 40% of the poorest households obtain about 41% of their food requirements from non-wood wetland resources a year. More than 76% of the population depend on medicinal plants from wetlands and about 43% use them as the only reliable source of medication. It is recommended that wetland development undertakings should explicitly understand and take into account the role of wetland resources to rural community’s’ livelihoods. In addition, there is a need of having a properly organised process in wetland resources utilisation. ************** 712 University of Dar es Salaam IMPACT OF PARTICIPATORY FOREST MANAGEMENT (PFM) ON POVERTY REDUCTION AND FOREST GOVERNANCE The Case of Suledo Forest Reserve, Kiteto District Manyara Region Alexander Songoro Master of Science (Natural Resource Assessment and Management), November 2007 This study examines the impact of PFM on poverty reduction and forest governance. Specific objectives included the assessment of the extent to which forest adjacent communities depend on the forest for livelihood; and investigating the impact of PFM on poverty reduction; and examining if the PFM has improved forest governance at local level. The study was carried out in Suledo Forest Reserve in Kiteto District Manyara region. Data collection was done through questionnaire interviews and a checklist, discussion with key informants, direct observation, and documentary review. The results indicated that about 96 % of respondents were aware of PFM; and about 95 % of them noted that, before the start of PFM, the forest was used without any proper planning, that led to degradation. About 61 % of the respondents reported that their income was negatively affected by the introduction of PFM. However, improvement is seen on non-income poverty including water sources, communication, gender equity and improved environment in general. Moreover, this research noted weaknesses on governance, especially on benefit-sharing mechanisms, whereby about 96 % of the respondents had no information about how revenue from SFR is utilized and handled. Following the above findings it is recommended that, PFM should go hand in hand with improvement of agriculture and other income generating activities. Funds obtained from timber harvesting must be handled properly. There is a need also to mobilize funds and other resources to support PFM. People should be made aware of the importance of PFM on poverty reduction. ************** A STUDY ON THE EFFECT OF DOMESTIC SEWAGE LOADING AND INUNDATION TIME ON POLLUTANTS REMOVAL EFFICIENCY OF TWO MANGROVES SPECIES (Avicennia marina and Rhizophora mucronata) Ally Kassim Kondo Master of Integrated Environmental Management (MIEM), November 2007 A bucket experiment was conducted to evaluate the effect of domestic sewage loading and inundation time on pollutants removal efficiency of Avicennia marina and Rhizophora mucronata mangroves species. Domestic sewage from the University of Dar es Salaam primary facultative waste stabilization pond was used to load onto buckets planted with the two mangroves species and unplanted buckets (bare sediment) were used as a control unit. Sea water was used to dilute the sewage to make desired sewage strength of 25%, 50%, 75% and 100%. Inundation times of 1 week, 24 hours and 12 hours were used in this 713 CONAS experiment. Based on the obtained results, both planted and unplanted units showed maximum removal of phosphates, total phosphorous and ammonium-nitrogen at 100% domestic sewage concentrations when inundated for 1 week. Moreover, a significant reduction in concentration of phosphorous, total phosphorous and ammonium-nitrogen was observed in planted units. While Avicennia marina units removed phosphorous, total phosphorous and ammonium-nitrogen by 94.49%, 93.25% and 94.76% respectively, removal efficiency of the same pollutants by Rhizophora mucronata units were 94.00%, 92.82% and 94.05% respectively. Generally, the removal percentage of phosphorous, total phosphorous and ammonium-nitrogen were significantly higher in 1 week inundation time than in both 24 hours and 12 hours inundation time. The overall results showed that, the two mangrove species could be used as phytoremediators of domestic wastewater in mangroves constructed wetlands in coastal areas. However, Avicennia marina units performed better than Rhizophora mucronata units in the phytoremediation of domestic wastewater. ************** INVESTIGATION ON THE PROPAGATION POTENTIAL OF DALBERGIA MELANOXYLON GUILL. & PERR. (AFRICAN BLACKWOOD) PROVENANCES AND THEIR GENETIC DIVERSITY FROM SELECTED AREAS OF TANZANIA Ezekiel Amri Doctor of Philosophy (Botany), September 2009 The objective of this study was to investigate means of optimizing both vegetative and seed propagation techniques and evaluation of genetic diversity of Dalbergia melanoxylon Guill. & Perr. (African Blackwood) provenances from the Eastern part of Tanzania. The effect of cutting position, age of donor plant, rooting media and provenance were investigated. The effect of timing of seed collection on seed germination was also investigated. Randomly amplified polymorphic DNA (RAPD) analysis was used to determine the genetic diversity of D. melanoxylon populations. Significantly (p< 0.05) high rooting ability 71.11% was achieved for basal cutting position from juvenile donor plant treated with IBA at 300 ppm. River sand was significantly (P < 0.05) ranked the best rooting media for stem cuttings. Germination capacity and germination energy were significantly high for seeds collected 12 and 16 weeks after maturity. Significant variation in rooting ability and seed germination were revealed among the provenances of D. melanoxylon. RAPD primers amplified 87 scored bands which revealed 73·6 % polymorphism and cluster analysis using UPGMA formed four major clusters. The genetic diversity within populations was 63.6% and among population was 36.4%. It was concluded that basal position cuttings of the juvenile donor plants and river sand rooting media should be used for vegetative propagation. Timing of seed collection in D. melanoxylon should be from 12th to 16th week maturity to obtain seeds with high viability and germination capacity. Genetic diversity revealed in D. melanoxylon is still high, therefore domestication and sustainable utilisation was recommended for preservation of genetic diversity of this economically important tree. ************** 714 University of Dar es Salaam WILDLIFE UTILISATION AND LIVELIHOODS IN ENDUIMET WILDLIFE MANAGEMENT AREA, LONGIDO DISTRICT, TANZANIA Sospeter Charles Master of Science (Natural Resources Assessment and Management), November 2009 Community Based Conservation (CBC) represents a significant potential opportunity for rural people to diversify their livelihood portfolios. However, CBC in Tanzania, as elsewhere, has been criticised as generally designed with a view to securing conservation gains rather than maximising benefits and devolving decisionmaking powers to rural communities. This has led to plenty of disagreement on whether or not the CBC option is economically viable in terms of livelihoods diversification and devolution of power and authority of wildlife management from central government to local communities. The study employed the political ecology approach to: examine the way local communities of Enduimet have been involved in decision making in relation to wildlife utilisation in CBC programme; investigate the competing interest of actors and; assess the implication of wildlife utilisation for local livelihood in Enduimet Wildlife Management Area (EWMA). A combination of qualitative and quantitative research paradigms were employed where direct observation, key informant interview, Focus Group Discussion (FGD), documentary review and questionnaire were used. Data were collected from a sample of 143 out of 1,787 households, randomly selected in four villages with a sampling intensity of 8%. The findings have revealed that, despite a considerable part of the income going into individuals’ pockets, there were significant benefits realised by villagers from photographic tourism investors. The benefits included improvement of social services such as classrooms and health facilities. Although 69.2% of the respondents reported being excluded from the decision-making process resulting in conflicts, the majority (74%) had a positive perception of the CBC programme. They still hope that things might be rectified and in the long run they would derive more benefits from their natural resources as expected. The study recommends that all impediments, including lack of community involvement, corruption and inadequate awareness of WMA, should be addressed immediately by the responsible ministry in collaboration with the community members. The DW should work together with the respective conservation NGOs, the District Council and representatives from villages forming EWMA in planning of the benefit sharing scheme which will satisfy local people’s needs and expectations for the purpose of sustainability of wildlife, tourism, people’s livelihoods and economic gains to the country. ************** GROWTH AND REPRODUCTION OF PALAEMONID SHRIMP PALAEMON CONCINNUS AND MORPHOMETRIC DIFFERENTIATION OF THE SESARMID CRAB PERISESARMA GUTTATUM IN SELECTED MANGROVES ALONG THE COAST OF TANZANIA Jairos Mahenge Master of Science (Aquatic Science), November 2009 715 CONAS Population structure and reproductive potential of Palaemon concinnus in Ruvuma estuary and the application of geometric morphometrics techniques to differentiate the carapace shape of Perisesarma guttatum for assessing connectivity for populations found in Dar es Salaam and those in Mtwara were studied. Samples of P. concinnus were collected on a monthly basis from May 2006 to April 2007 and once for P. guttatum. Samples were preserved in 70% ethanol. P. concinnus individuals were sorted into ovigerous, non-ovigerous and sexed. The carapace length and body weight were measured and embryo developmental stage examined prior to counting the eggs. The carapace for each sex of P. guttatum was photographed and the file utility program Tps was used to record landmarks followed by a series of analysis. The carapace length and body weight of P. concinnus ranged from 1.71 mm to 9.18 mm and 15.8 mg to 401.3 mg respectively. The slopes (b-values) of the carapace length–body weight relationship for males (2.42), females (2.64) and combined sexes (2.56) were significantly different from the isometric value (b = 3) hence negative allometric growth. Sex ratio was found to be 0.78:1 (male: female) and significantly different from 1:1 ratio (?2 = 40.611, p < 0.001). Fecundity ranged from 34 to 312 eggs and correlated positively with carapace length (p < 0.0001). The carapace shape of P. guttatum in females and males was found to be significantly different in both sites and stations (p < 0.001). However, some stations from the two sites did not show differences indicating possible connectivity of the gene pool. ************** CLIMATE CHANGE IMPACTS AND ADAPTIVE STRATEGIES IN THE RUFIJI DELTA Ronald Ndesanjo Master of Science (Natural Resource Assessment and Management), November 2009 The study aimed at investigating Climate Change impacts and adaptive strategies in the Rufiji Delta. The specific objectives included the following; to establish the local perceptions on climate and its associated changes, to establish evidences and/or indicators of climate change, to examine impacts of climate change on livelihood activities and to determine coping and adaptation strategies by local communities and other parties. The study employed descriptive design, together with qualitative and quantitative techniques of data collection. The data collection methods included interviews, questionnaires, Focus Group Discussions (FGDs) and documentary evidence. The study also took in a sample of households and Village Executive Officers. Findings of the study showed that livelihood activities in the study area particularly farming and fishing are already impacted by climate change. It was observed that most of the crops used to be grown have been abandoned with the exception of paddy that depend on irrigation. Similarly, fish catch was noted to have declined with disappearance of some of the species. On the other hand the study noted the increasing scarcity of basic household necessities especially food and water that was associated with the current frequent dry spells as well as altered rainfall patterns. The study also noted the impacts of sea water rise to settlements, underground aquifers and soils. Therefore, it is evident that the community is already impacted by climate change, a situation that calls for institutionalisation of such means as dry land agriculture, advanced fishing methods as well micro-crediting to enable such communities sustain their economies by engaging in secondary economic activities rather than relying upon nature. ************** 716 University of Dar es Salaam CLIMATE CHANGE AND ITS IMPACTS ON COASTAL TOURISM A Case of Bagamoyo District Roland Sylvester Mushi Master of Science (Natural Resource Assessment and Management), November 2009 This study assessed the impacts climate change on coastal tourism along the coast of Bagamoyo including Dunda and Kaole villages. It identified potential impacts on coastal resources that are used as tourist attractions, and assessed the vulnerability of coastal tourism, how it is adapted to climate change impacts. Further, the study examined the existing policies that are addressing mitigation and adaptation to climate change in Tanzania. The methodologies employed in this study include GIS and remote sensing analysis; this helped in detecting the vegetation covers change and shoreline change along the coast which showed that there is a gradual decrease of beach areas as well as landward recession. Purposive samplings for key informants as well as random sampling were used in the collection of data on local communities’ perceptions on the climate change impacts. Participant observation and literature reviews were extensively used. Results observed from TMA rainfall data from 1950-2007 showed that, the rainfall trend has been decreasing over the years and this has affect farmers as well. Temperature increased by approximately 0.50C and 2°C (for mean maximum and minimum temperature respectively) whereas the average annual temperature increases of 1°C for over 30 years since 1978 which cause discomfort to tourists as well as local communities. Findings from the study showed that, coastal natural resources are vulnerable to the increased temperature, sea level rise and drought. The threats from sea level rise and coastal erosion is of significant which increases vulnerabilities to coastal tourism activities in the areas. Further, the study revealed that existing policies and institutional frameworks do not explicitly address the mitigation and adaptation strategies to coastal tourism activities. And several adaptation strategies such as construction of sea walls, re-planting of mangroves trees and piling up of sand bag were noted to be applied by Antiquity Department and hotels. It is therefore, recommended that emphasis should be on adaptation strategies including institutional capacity building, re-afforestation programme, and enhancing public awareness and provision of education on climate change impacts to the local communities, tourism stakeholders and government officials. ************** ASSESSMENT OF TYPES AND LEVELS OF PESTICIDE RESIDUES IN SOME COMMONLY GROWN VEGETABLES IN TANZANIA Mary Mkony Meela Master of Science (Chemistry), November 2009 This study was conducted on the types and levels of pesticide residues found in florisil cleaned 144 extracts of cabbage (Brassica oleracea var. capital capitata), tomato (Lycopersicon esculenta,) amaranths (Amaranthus spp), Chinese cabbage (Brassica rapa), carrots (Daucus carota) and nightshades (Solanum vilosum), collected from Arusha, Iringa, Morogoro and Tanga Regions of Tanzania; which were analyzed 717 CONAS by GC-ECD and GC-NPD; and confirmed by GC-MS. The types of pesticide residues (and their percentage frequency of detection) were as follows: cabbage: ?endosulfan (35%), ? HCH (34%) and fenitrothion (21%); tomato: ? HCH (40%), fenitrothion (30%), ? endosulfan (20%) and ?DDT (10%); amaranths:?HCH (57%), fenitrothion (36%) and ?endosulfan (7%); chinese cabbage: ?endosulfan (33%), ? DDT (33%), ?HCH (22%) and (11%); carrot recorded frequency of detection of ? HCH (11%), ?DDT (44%) ?endosulfan (33%) and dieldrin (9 %). Cabbage samples showed a Codex MRL non-compliance of 50% whereas tomato recorded 8% non-compliance (due to the endosulfan in Iringa samples that was detected at concentration of 1.8 ?g/g against 0.5 ?g/g MRL); Chinese cabbage had a non compliance of 45%. While carrot had all samples (100%) being codex MRL non compliant, Amaranths and nightshade, both of which have no Codex MRL had residues above the detection limit in all the samples. There was significant variation in the concentrations of pesticide residues detected in different samples from different sites in both the dry and wet seasons. ************** VULNERABILITY ASSESSMENT OF RAIN-FED AGRICULTURE TO CLIMATE CHANGE AND VARIABILITY IN SEMI-ARID AREAS OF TANZANIA The Case of Tabora Urban and Uyui Districts Hector John Mongi Master of Science (Natural Resource Assessment and Management), November 2009 This study assessed vulnerability of rain-fed agriculture to climate change and variability in four clustered villages, three from Uyui District and one from Tabora Urban District. Villages studied in Uyui were Mbola, Isila and Mpenge while in Tabora urban was Tumbi. Both secondary and primary data on biophysical and socio-economic aspects were collected using different approaches including structured interviews, focus group discussion, documentary review and field observations. Structured interviews were administered to 7% of all farmers selected at random from the four villages and 30 research and extension officers obtained through accidental purposeful sampling. Simple regression and t-test analyses of numeric data for rainfall and temperature collected over the last 35 growing seasons were performed using MS Excel and SAS respectively. Non-numeric data were coded, summarized and analyzed using SPSS spreadsheet. Results showed that overall rainfall amount was declining and distribution was varying both in time and space. Inter-seasonal dry spells between January and February appeared to increase both in duration and frequency. Increase in temperature was highly significant (p<O.Ol), however, with minimum temperature increasing much faster than maximum. Farmers, research and extension officers also perceived these changes by the help of a series of indicators. Nevertheless, perception on the climate change indicators varied depending on the type of livelihood activity most affected. Major implication on rain-fed agriculture included possible shrinking of the growing season, increasing moisture and heat stress to common food and cash crops, increased insects and disease pests and eventually low income and food security. Basing on the results above, this study concludes that there is strong evidence demonstrating the vulnerability of rain-fed agriculture to negative impacts of CC&V in the study area. Among recommendations made is a need for multi-level interventions on adaptation to climate change and variability taking into account a wide range of stakeholders’ involvement. ************** 718 University of Dar es Salaam BIOACTIVITY OF SECONDARY METABOLITES FROM MICROBES ISOLATED FROM IDUNDI, TRADITIONALLY FERMENTED BANANA FROM TANZANIA Amina Msonga Master of Science (Molecular Biology), September 2009 Idundi is a traditionally fermented banana food usually consumed by women in North-eastern Tanzania soon after child birth, believed to help in the healing of their wombs. This study was aimed at investigating the in vitro bioactivity of secondary metabolites produced by microorganisms associated with idundi. Activity was investigated by agar well and paper disk diffusion methods and broth microdilution method. A total of 3 bacteria and 6 filamentous fungi were isolated from the traditional fermentation process of bananas in Mbaga village, Same, Kilimanjaro. Out of these, 4, (44%) produced bioactive medium polar extracts with a varying degree of antimicrobial activity against Escherichia coli, Staphylococcus aureus, Candida albicans, Aspergillus niger and Cryptococcus neoformans known to be human pathogens. Two of the bacterial isolates producing bioactive extracts were identified to be Klebsiella oxytoca and Kluyvera cryocrescens on the basis of their 16S rDNA sequences. Two of the filamentous fungal isolates producing bioactive extracts were identified to be Trichoderma aureoviride and Rhizopus stolonifer on the basis of their ITS region sequences. Two yeast isolates namely; Candida pyralidae and Rhodotorula mucilaginosa were found to produce bioactive extracts which were active against the test organisms. Chemical profiling of the extracts revealed the presence of alkaloids, terpenoids, phenolics and sterols from the bacterial and fungal isolates and alkaloids and terpenoids from the yeasts isolates. In conclusion, antimicrobial activity of secondary metabolites from microbes associated with idundi provides scientific corroboration for its therapeutic value. ************** FEASIBILITY OF BLACKLIP OYSTERS (Pinctada margaritifera, Linnaeus, 1758) HALFPEARL CULTURE IN TANZANIA Edson Ishengoma Master of Science (Marine Biology), July 2009 There was a need for some ecological and biological information on which the feasibility of pearl culture in Tanzania could be assessed. The major objective of the project focused on where, when and how to obtain pearl oyster spat (Pinctada), where to grow them and trial production of half-pearls. Three spat collection sites were selected randomly and these were Tawalani, Bweleo and Nyamanzi. Seasonal variation (wet and dry) of the spat settlement was determined by establishing the long-line at each site, and spat collectors deployed and harvested during wet and dry season of the year. The quantities of spat settling on spat collectors both in wet and dry season were compared. In just the same experiment, 719 CONAS the effectiveness of spat collection methods was determined by deploying at the same moment three different spat collector materials (coconut shells, rubber slides and spat bags) and comparing which of these had better spat number. Spat growth was experimented and responses compared at 2 m and 6 m.. And mature and health oysters were implanted with two different sizes (small and big) of the nuclei to determine the effect of the size of the nuclei on the survival of oysters and on the quality of resulting halfpearls. The spat collection experiment produced in total of 4263 Pinctada margaritifera spat over a year among the selected sites. Seasonal variation in spat “yield” was observed at each studied site, the dry season yielding higher number of spat than the wet season and the Nyamanzi site yielding significantly poor number of spat in total compared to the other sites (P < 0.05). Coconut shells were significantly the least effective method at collecting spat, compared to spat bags and rubber slides (P < 0.01). Spat growth response in terms of dorso-ventral height (DVH) was significantly better at 6 m than at 2 m (P < 0.05) after 90 days of rearing. The survival of implanted oysters was significantly better with oysters implanted with small nuclei (0.297± 0.244) than oysters implanted with big nuclei (0.619± 0.209) during the initial 30 days. The percentage of quality and non-quality half-pearls in small and large nuclei did not differ significantly (P > 0.05). The findings of this study indicated that it is feasible to obtain pearl oyster spat, grow them successfully and produce relatively good quality half-pearls within a short period of 9 months using P. margaritifera. However, there is a requirement for further research to appraise the consistency of spat availability over a long term and the feasibility for round pearl production. ************** INVESTIGATION OF THE GENETIC DIVERSITY OF MORINGA OLEIFERA PROVENANCES AND OTHER MORINGA SPECIES FOUND IN SELECTED COASTAL REGIONS OF TANZANIA. Mariam Mgendi Master of Science (Botany), August 2009 Knowledge of population genetic diversity is one of the prerequisites for development of plant species conservation strategies. It is also fundamental to the understanding of ecology and evolutionary forces that affect them. In the case of wild species that represent the genetic resources for cultivated plants, the understanding of their genetic diversity is essential as they are the source of genetic material that are used to improve elite varieties. This study was conducted to investigate the morphological and genetic variations between and within cultivated and non-cultivated provenances of Moringa oleifera Lam. and to explore possible existence of other Moringa species in selected coastal regions of Tanzania. Ninety and ninety six individuals from cultivated and non-cultivated populations were assessed for morphological and molecular analysis respectively. The morphological and genetic relationships were analyzed using NTSYS-pc and UPGMA programme. Morphological analysis revealed six clusters with similarity ranging from 66% to 100% while Genetic analysis using 12 selected RAPD primers revealed five clusters with similarity ranging from 54% to 96%. Genetic distance within M. oleifera group ranged from 65% for most divergent individuals 720 University of Dar es Salaam to 95% for most related individuals. The genetic distance within the majority of cultivated populations ranged from 76% to 93% and 80% to 96% in the majority non-cultivated populations. Both morphology and RAPD data partly separated individuals based on the status of individuals whether cultivated or noncultivated. Furthermore, genetic separation between M. oleifera and M. stenopetala supports the occurrence of two species of Moringa in Tanzania. ************** MATHEMATICAL ANALYSIS OF A CHOLERA MODEL WITH CONTROL MEASURES Mwasa Abubakar Master of Science (Mathematics), July 2009 Cholera is an acute diarrhoeal illness caused by infection of the intestine with bacterium Vibrio cholerae (V. cholerae). The aim of this study is to use mathematical modeling to analyze the dynamics of cholera disease with and without control measures. The mathematical features such as the epidemic threshold, equilibria and stabilities are determined for different sub-models and for the full model (with control measures). A Lyapunov functional approach is used to analyze the stabilities of equilibria. We qualitatively analyze existence and positivity of solutions. The possibility of backward bifurcation is investigated and it is observed that the model does not exhibit such phenomenon. We use the model to study the effects of public health educational campaigns, Vaccination as control strategies in curtailing cholera disease. The education-, vaccination-, treatment-induced reproductive numbers RE, RV , RT respectively and combined reproductive number RC are compared with the basic reproduction number R0 for cholera in the absence of any intervention to assess the possible community benefits of these control measures. We perform sensitivity analysis on the key parameters that drive the disease dynamics in order to determine their relative importance to disease transmission and prevalence. We also use numerical simulations to validate the model and qualitatively show the benefits of these control measures, and we investigate the effect of seasonal variation of the contact and contamination rates _ and ‘, respectively. We conclude from the study that in settings where education and vaccination are effective and with reasonable provision of clean drinking water and clean environment, the average number of infected humans may be reduced. ************** 721 CONAS A MATHEMATICAL MODEL OF RIFT VALLEY FEVER WITH HUMAN HOST Saul Chilongola Mpeshe Master of Science (Mathematics), July 2009 We formulate and explore a deterministic SEIR model of Rift Valley Fever (RVF) with mosquitoes, livestock, and human host using a deterministic system of nonlinear ordinary differential equations. We analyse the model to find the basic reproduction number, 0 , the stability of the disease-free-equilibrium • (DFE) and endemic equilibrium (EE), and to test which model parameters are more sensitive to 0 which • is the measure of initial disease transmission and EE which is the measure of disease prevalence through sensitivity analysis. We find that the DFE is locally asymptotically unstable when 1 0 , and the EE islocally and • • globally asymptotically stable when 1 0 . Both 0 and the disease prevalence in mosquitoes are • • • more sensitive to the natural death rate of mosquitoes, m d , and this suggest the use of mosquito treated bednets and sprays to increase m d , and hence a reduction of 0 • and the disease prevalence. The disease prevalence in livestock and humans are more sensitive to livestock recruitment rate, l , and • human recruitment rate, h , respectively, thus suggesting an isolation of livestock from human population. • The sensitivity indices of 0 and EE with respect to the parameters l and h calls for thorough • • • diagnosis and treatment strategies for infectious individuals to reduce disease prevalence. Finally, we perform numerical simulations to explore the behaviour of RVF when introduced in a system with constant recruitment rate in both populations. ************** DYNAMICS OF FISHERIES WITH PREY RESERVE AND HARVESTING Sara Abdulla Khamis Master of Science (Mathematics), July 2009 A model for two fish species (preys) and one predator in a patchy environment is formulated using non linear differential equations to study the dynamics of marine fishery resources in two homogeneous patches; one is a free fishing zone and the other is a refuge for prey reserve in which fishing is prohibited. Biological behavior of the system is examined, the system is analyzed around non-negative steady states and the criteria for local and global stabilities are derived. The possibility of existence of bionomic (biological as well as economic) equilibrium of the system is determined and the conditions for their existence are derived. The optimal harvesting policy is studied by using the Potryagin’s maximal principle and the optimal equilibrium solution is obtained when the total user’s cost per unit effort equals the steady state effort level. The sensitivity analysis is carried out and it is observed that the populations are more sensitive to growth, dispersal and predation rates and least sensitive to the catchability coefficient. Statistical analysis (Markov Chain Monte Carlo) is employed to estimate the parameters and to assess both the uncertainty 722 University of Dar es Salaam in the model parameters and the uncertainty in the model-based predictions. Some graphical representations of the numerical simulations using MatLab are presented to illustrate and complement the analytical results. ************** AN ASSESSMENT FOR THE HYBRIDIZATION POTENTIAL OF WILD COTTON WITH CULTIVATED COTTON AS A PRELUDE TO THE INTRODUCTION OF TRANSGENIC COTTON Oshingi Shilla Master of Science (Biotechnology), February 2009 To increase cotton production and improve the national economy, Tanzania wants to revive cotton production in Southern Highlands (SH). The SH was quarantined since 1968 as a management strategy against the Red bollworm. Genetically modified (GM) cotton with insecticidal properties is a potential solution to the bollworm infestation. GM crops pose environmental concerns warranting ecological risk assessment (ERA) be done prior to their introduction. In Tanzania, draft Biosafety regulations (Tanzania, 2007) call for ERA to be conducted prior to GM cotton introduction. ERA inputs include baseline information on diversity and distribution of wild /feral relatives of cotton and reproductive mechanisms that Tanzania is presently lacking. This study assessed feral Gossypium barbadense diversity and distribution in the SH through a field survey and assessment of interrelationship by controlled hybridization with cultivated Gossypium hirsutum. A literature review from two herbaria and cotton research stations revealed scanty diversity and distribution of wild/ feral cotton. The field survey, identification and clustering revealed that G. barbadense, originated from Brazil and is compatible to G. hirsutum. However, the evaluation showed some phenotypic variations within plants collected suggesting that G. barbadense may have hybridized with cultivated varieties. Regardless of the variations, feral G. barbadense had great affinity to cultivars producing fertile F1. The hybridization was significant at X2 of 234.6 for test of independence and 157.4 for a trend both at P < 0.0001. The parent-offspring relationship was confirmed by Simple Sequence Repeat (SSR) molecular markers. SSR markers have shown to be useful in detecting paternity and are recommended for similar studies. ************** ROLE OF SMALL SCALE COMMUNITY-BASED MARINE NO-TAKE AREAS IN CONSERVATION OF Anadara antiquata LINNAEUS 1758 (PELECYPODA: ARCIDAE) IN FUMBA PENINSULA, UNGUJA ISLAND Redfred Geofrey Ngowo Master of Science (Aquatic Science), May 2009 723 CONAS Cockle population is threatened by higher dependence of community members as they are used as potential source of food and income. This study was assessing the role of small scale community – based marine notake areas in conservation of cockles (A. antiquata) was through before - after (BA) and Reserve – Adjacent – Control (RAC) pairs method in three sites of Nyamanzi, Bondeni and Chaleni in Unguja Island. Biological studies on morphometry and reproduction of A. antiquata were also conducted to assess their reproductive output and factors affecting them. The mean density of cockles ranged between 2.27 0.41 SE in reserves, 1.85 0.34 SE in adjacents and 0.76 0.07 SE in control sites. A. antiquata • • • were found to attain their length at first maturity at 33 mm, 32 mm and 37 mm for female, male and hermaphrodites respectively. Examination of gonads of fully matured female showed that a mean fecundity was 2,840,000 1 1 SE eggs per individual, and the number of eggs was found to increase with the increase in total length of A. antiquata. Small scale community based marine no-take areas is important tool for management of A. antiquata and other molluscan bivalves. For effective protection of bivalve stocks and species biodiversity, this study is recommending having an integration of management strategies through establishment of communitybased no-take areas, and protection of brood stocks by setting 48 mm SL as minimum size for the cockle to be collected. ************** AUDITING OF FISH WASTES GENERATED ALONG LAKE VICTORIA AND ENHANCEMENT OF ANAEROBIC DIGESTION OF FISH PROCESSING WASTEWATER Robert Gumisiriza Master of Science (Biotechnology), January 2009 In East Africa, Nile perch processing into fish fillet for export generates large proportions of both solid and liquid fish wastes, which are improperly utilized and/or disposed off untreated leading to environmental pollution problems especially on Lake Victoria. Through auditing, it was estimated that 36,000 tonnes of fish solid waste and 1,838,000 m3 of Nile perch fish processing wastewater (FPWW) were generated annually. The FPWW was of high strength with total chemical oxygen demand (COD) of 12,400 mg/l and Total solid content of 5,580 mg/l. The wastewater contained 6,160 mg/l of lipids and 2,000 mg/l of proteins. The FPWW generated annually contained 10,290 tonnes of total solids, of which 9,820 tonnes (95 % TS) were volatile solids (VS). Batch wise anaerobic digestion (AD) of FPWW at laboratory scale yielded 560 m3 CH4/ton VS added; implying that the total annual wastewater generated can potentially yield 5,498,960 m3 CH4/year. Extrapolation from the methane yield indicated that the total annual FPWW generated had diesel oil and electricity production potentials of 5,498,960 m3 and 55 Gwh, respectively. Co-digestion of the residue with 10 % gVS of brewery wastewater optimally enhanced methane yield to an increment of 66 % while pretreatment of FPWW with aerobic microbial strains locally isolated from a fish waste stabilization pond enhanced methane yield to an increment of 76 % after 12 hours of incubation with mixed cultures. Results of this study indicate that fish processing can be energy self –sufficient through biomethanation of FPWW and substrate pre-treatment enhances the AD process. Further investigations on the effect of substrate pretreatment at a scaled up level are encouraged. ************** 724 University of Dar es Salaam DEVELOPMENT OF A FLEXIBLE WEB-BASED EXPERT SYSTEM FOR DIAGNOSIS OF ANIMAL DISEASES IN TANZANIA Ntwa Andalwisye Katule Master of Science (Computer Science), August 2009 This research investigates how a flexible web based expert system for diagnosis of animal diseases should be developed to allow domain experts in the field of veterinary medicine to modify the knowledge base i.e. editing existing knowledge, removing obsolete knowledge, or integrating new knowledge into the knowledge base. It also investigates how knowledge should be inferred from the dynamic knowledge base when end users consult an expert system with intention of seeking for an advice. The developed system is a prototype of flexible web based expert system for diagnosis of animal diseases based on symptoms. The methodology involves elicitation of first hand knowledge from veterinary experts, which was required for modelling of the knowledge base shell. Object oriented analysis and design approaches were used to model and design an expert system shell specific to the domain of animal diseases’ diagnosis. The System was implemented in Java, which is an object oriented programming language, and in Prolog, which is an artificial intelligence programming language. The knowledge base was implemented by using Myoodb database management system. An inference engine uses facts loaded dynamically from the knowledge base to deduce the diagnosed disease. By using a simple Bayesian belief network, an expert system consultation part has the capability of reasoning with uncertainty knowledge hence improving its intelligence. All user interfaces are web-based. A Knowledge acquisition subsystem has been included to allow maintenance of the knowledge base. The system was verified by veterinary doctors as end users. The developed prototype can be extended to become a full functioning system to support veterinary extension services. ************** THE ROLE OF INDIGENOUS KNOWLEDGE IN ADAPTATION TO CLIMATE CHANGE AND VARIABILITY A Case of Bagamoyo District, Tanzania Berlinson Andrew Master of Science (Natural Resources Assessment and Management), November 2009 This study investigated the role of indigenous knowledge (IK) in adaptation to climate change and variability in two villages selected from two agro-ecological zones in Bagamoyo district. Data was collected by using key informants interviews, focus group discussions, household survey as well as review of secondary data. The Statistical Packages for Social Sciences (SPSS) and Excel were used to analyze quantitative data. Qualitative data were analyzed by cross tabulation and comparison of different views. Results were presented 725 CONAS in descriptive forms: tables, figures, graphs, maps and photographs. The study found out that through IK, the communities studied have developed several indigenous adaptation strategies that have enabled them to reduce their vulnerability to past and present climate change and variability. These strategies were particularly evident in farming, fishing, food preservation and storage and in dealing with natural disasters like droughts and famine. It is concluded that the communities studied have a high awareness of climate issues and through their IK, these communities have managed their resources and livelihoods in the face of challenging environmental and socio-economic conditions. Therefore, there is a need of recognizing and actively promoting IK through incorporating it into climate change policies and modern knowledge. Incorporating IK can add value to the development of sustainable climate change adaptation strategies that are rich in local content, and planned in conjunction with local people. ************** COMMUNITY-BASED FOREST MANAGEMENT WITHIN THE CONTEXT OF INSTITUTIONAL DECENTRALIZATION: CHALLENGES AND OPPORTUNITIES IN UPPER SONGWE RIVER BASIN Kayonko Juma Master of Science (Natural Resources Assessment and Management), December 2008 This study was undertaken to assess community-based forest management (CBFM) within the context of institutional decentralized: challenges and opportunities in upper Songwe River basin (USRB). The results contribute to the understanding of the needs for sound forest management in USRB where anthropogenic catchment damage has caused severe erosion and downstream siltation into Lake Nyasa/Malawi. Satellite image interpretation was used to establish land use and cover change in USRB. Information on the dynamics of forest management was obtained through interviews and focus group discussions. Satellite image interpretation revealed substantial degradation and decline of natural forests in the USRB, from 3,564ha in 1991 to 2,252ha in 2004, due to agricultural and settlement expansion. Lack of effective forest management plans, mismatch between ecological and administrative boundaries, and wrecked inheritance of traditional environmental knowledge and practices caused by change in population composition and characteristics poses extended challenges for the establishment of formal CBFM. Opportunities are rather rare but perceived future ecological and socioeconomic benefits revealed a significant relationship (x²=33.913, df=18, a=0.05) with the support of formal CBFM of the remaining natural forests in the USRB. Such findings go in front of the conclusion that the scaling up of CBFM will depend not only on a supportive policy and legislative environment, but also on suitable socioeconomic conditions embedded in the history of forest management of a given community. Sustainable CBFM interventions ought to go with the development of additional income-generating community-based projects so as to improve the socioeconomic well-being of the people while at the same time reducing pressure to allow the recovery of the natural forest ecosystem in the USRB. ************** 726 University of Dar es Salaam A GIS-AIDED AEROGEOPHYSICAL, GEOLOGICAL AND GEOCHEMICAL INVESTIGATION OF LATE ARCHAEAN GRANITOIDS IN THE MUSOMA-MARA GREENSTONE BELT, NORTH EAST TANZANIA Elisante Elisaimon Master of Science (Geology), August 2008 The Musoma Mara Greenstone Belt (MMGB) is intruded by syn-to-post orogenic suites of granitoids which range in composition from Tonalite Trondhjemite Granodiorites (TTG) to granite. High resolution aerogeophysical survey by the Geological Survey of Tanzania in 2003 has provided aeromagnetic and radiometric data that were used in this study to classify various granitoids existing in the MMGB. Based on the analysis and interpretation made on airborne radiometric and magnetic data as well as previous geological maps, a geophysical interpretation map was obtained. This map broadly categorized the MMGB granitoids into two types, the first granitoid type is characterized by high contents of K, U and Th and magnetic intensity < 33997 nT. The second granitoid type is characterized by high K relative to U and Th, and magnetic intensity >33997 nT. The aerogeophysical interpretation map was used as a base map for ground follow-up whereby the granite types were sampled accordingly for geochemical analysis. The two granitoid types identified on the basis of geophysical data can further be subdivided into three types based on geochemical data i.e. biotite granites, calcic granites and TTG. The more abundant biotite and calcic granites correspond to the aerogeophysical high K, U and Th contents whereas the TTG correspond to the high K relative to U and Th concentrations. On the basis of compositional similarity with the northern MMGB high-K and Na-rich granitoids, that were previously studied by Manya et al (2007a, b), the biotite and calcic granites are inferred to have been generated by partial melting of pre-existing materials including TTG and intermediate to felsic volcanic rocks whereas the TTGs were generated by partial melting of hydrous basaltic crust that had been metamorphosed to garnet amphibolite. ************** ASSESSMENT OF PHYSICO-CHEMICAL PARAMETERS AND THEIR IMPACTS ON THE WATER QUALITY AT IGOMBE RESERVOIR, TABORA, WESTERN TANZANIA Jacqueline Mwendwa Master of Science (Environmental Sciences), October 2007 Assessment of physico-chemical parameters and their impacts on water quality at Igombe reservoir was done by investigating the influence of anthropogenic activities on water quality during wet and dry seasons. Standard methods were used to determine the levels of physico-chemical parameters from the water samples. Physical parameters including temperature, turbidity, alkalinity, dissolved oxygen 727 CONAS (DO), electrical conductivity (EC), and pH, were determined in situ. Chemical parameters such as NO3, SiO2, PO43-, Fe2+, SO42-, Cl-, and major cations were determined in the laboratory. Data show higher concentration of physico–chemical parameters during the wet season than during the dry season at various sampling sites. This has been attributed to reservoir-increased input of the physico- chemical parameters by surface runoff. The mean variation of NO3-, SiO2, PO43-, Fe2+, SO42-, and Cl- concentrations ranged from 0.7 to 2.9 mg/l, 11.38 to 34.9 mg/l, 0.02 to 0.27 mg/l, 0.04 to 0.86 mg/l, 6.3 to 7.8 mg/l, and from 5.2 to 6.5 mg/l respectively. Data have shown that processes including dissolution, diffusion, adsorption, absorption, nitrification, denitrification, mixing and reduction along with anthropogenic activities explain the variation of physico-chemical parameters in the reservoir. It has been concluded that human activities impacts significantly on the water quality of the reservoir. It is recommended that continuous monitoring and surveillance of anthropogenic activities taking place around the Igombe reservoir should be conducted in order to control pollution from both point and non point sources to ensure sustainable water resource at the reservoir ************** GENETIC VARIABILITY AND PHYLOGENY OF YEASTS FROM SEDIMENTS OF SELECTED MANGROVE ECOSYSTEMS OF COASTAL REGIONS OF TANZANIA Eva M. Sosovele Master of Science (Molecular Biology), November 2008 The aim of this thesis was to improve the understanding of the types of yeasts associated with mangrove sediment along the Coast of Tanzania and how they have been impacted by sewage pollution. Molecular techniques such as Random amplified polymorphic DNA (RAPD) PCR and analysis of base sequences of ribosomal DNA (rDNA) were used to establish the taxonomy, phylogeny, genetic variability and impact of anthropogenic activities on isolated yeasts. A total of 43 morphologically different yeast isolates from various mangrove sediment samples from Dar es Salaam, Lindi and Tanga regions were investigated in this study. RAPD PCR results revealed closer genetic affinities between Tanga and Lindi yeast genotypes. This study has also observed that, the anthropogenic activities have modified the relative variability of mangrove yeasts genotypes. Phylogenetic analysis of the yeasts in this study based on rDNA sequences revealed that, the yeasts such as Candida species (typically found in polluted mangrove sediments) appear to have evolved earlier and clustered with other Candida species from elsewhere in the world. When the Rhodotorula mucilaginosa and Galactomyces geotrichum (isolated from unpolluted regions of Tanzania) were subjected to the phylogenetic analysis, they also clustered together with other respective yeast species from other parts of the world, and they evolved earlier than most of Rhodotorula species and Galactomyces species from other parts of the world. This study established that, the yeast species isolated from Tanzanian mangrove sediments are similar with those found in mangrove sediments of other parts of the world and the sewage pollution affects their genetic structure. ************** 728 University of Dar es Salaam ASSESSMENT OF GENDER RELATIONS IN IRRIGATED AGRICULTURE IN KILOSA DISTRICT, MOROGORO REGION, TANZANIA Tumaini Allan Master of Science (Natural Resource Assessment and Management), November 2008 The study on gender relations in irrigated agriculture was undertaken at three irrigation schemes in Kilosa District. The main objective of the study was to assess gender relations in irrigated agriculture for equitable access to resources in selected irrigation schemes. The methodology utilized key informant interviews, focus group discussion and questionnaire interviews. The sample consisted 3 irrigation schemes whereby 20 men and 20 women were selected randomly from each scheme. Quantitative data were analyzed using SPSS and Microsoft Excel program while ANOVA test was done for statistical analysis. On average, the proportion on water committees was 29% women and 71% men, which influenced decision-making on water allocation in favour of men in the irrigation schemes. Statistical analysis showed that the difference between mean acreage owned by men and women at P=0.033 was statistically significant different. Analysis of roles and responsibilities of men and women showed that were gendered. Men were doing land preparation, watering, collecting crops from farm and selling crops. Women were responsible for sourcing seeds, planting and scaring birds including wild animals (monkeys) on the farm. Joint activities included weeding and harvesting. Men were involved in cash crop farming for sale while women were greatly involved in food crop farming for household consumption. Hence, income earned from farming and nonfarming activities differed between men and women (P=0.019). Factors causing existing gender roles in farming activities were grouped into categories such as socio-cultural, economic, educational and accessibility of information and political factors. To mainstream gender in irrigated agriculture, there is a need to ensure democratic election of leaders in village government and in other resource allocating committees. Apart from gender-related attributes, other cross-cutting factors crucial for improving irrigation agriculture include, increased water availability through infrastructure improvement, rainwater harvesting, improved market and credit facilities for farmers. Farming and resource management skills of both genders are critical so as to attain sustainable development on an equal footing as per Millennium Development Goals. ************** POPULATION DISTRIBUTION AND DIFFERENTIATION OF MANGROVE AND ROCKY SHORE GASTROPODS (LITTORARIA SPP.) IN DAR ES SALAAM, MNAZI BAY-RUVUMA ESTUARY AND INHACA ISLAND IN MOZAMBIQUE Rose Ephraim Sallema Master of Science (Aquatic Science), November 2009 This study assessed population distribution and differentiation of mangrove and rocky shore littorinid species (Littoraria scabra, L. pallescens, L. subvittata, L. intermedia and L. glabrata) in Dar es Salaam, Mnazi Bay-Ruvuma Estuary and Inhaca Island. Nested sampling design was employed in assessing spatial scale variation of Littoraria species. For littorinids distribution study, sampling was conducted in wet and dry seasons whereas in population differentiation study, sampling was done only once. 729 CONAS The current study revealed a significant difference in littorinid population distribution and differentiation at both low and high spatial scales. Distribution of mangrove trees and seedlings indicated great variation in the zones; however, no variation was noted for saplings. Male littorinids were revealed to be fewer in number than females of the same species in all mangrove zones. Littorinid abundance varied significantly among zones in different mangrove heights (specific levels) and substrates. No significant difference in mangrove basal area was found among seasons and sites; however, there was great variation among zones. There was a significant correlation between littorinid abundance and mangrove abundance and basal area. Males and females of L. glabrata and L. scabra exhibited different population differentiation patterns at both low and high spatial scales. Results of the study suggest that large scale variation might reflect changes in physical factors possibly associated with habitat fragmentation, whereas small scale variations might suggest differential responses of littorinids to prevailing habitat conditions in each floristic zone/rocky shore habitats. ************** THE ROLE OF NON-TIMBER FOREST PRODUCTS IN RURAL LIVELIHOODS IN ANGAI VILLAGE LAND FOREST RESERVE Someni Lusungu Mteleka Master of Science (Natural Resources Assessment and Management), November 2008 The general objective of this study was to assess the contribution of NTFPs to the livelihoods of communities adjacent to Angai Forest Reserve and implication of their utilization of the NTFPs on forest management. The study was conducted in two phases. Phase one involved preliminary surveys and participatory rural appraisals in three villages, Kiangara, Kitogoro and Liwale “B” adjacent to Angai Village Land Forest Reserve in Liwale district. The second phase involved a questionnaire survey conducted with a sample size of 11% of the households of the sampled villages. Data collected through the questionnaire survey were analyzed by using both quantitative and qualitative methods. NTFPs identified were firewood, charcoal, poles, withies, ropes, wild vegetables, wild medicinal plants, tubers and roots, mushrooms and bush meat from animals and birds. The study revealed that the average value of NTFPs used by the household per year was TAS 821,250, which contributes about a 40% of the total household income of TAS 2,027,020. It was noted that NTFPs play an important role in meeting subsistence needs of the adjacent communities. It is recommended that there is need to empower local communities by increasing their capacity to monitor and manage their forest resources considering their local knowledge of NTFPs. A detailed inventory of NTFPs is needed in order to establish a resource base that will facilitate the development of a harvesting plan. Domestication of NTFPs through agro-forestry and afforestation of suitable tree species should be encouraged that will act as a substitute for the indigineous trees species greatly used in the study area. ************** 730 University of Dar es Salaam GEOLOGY AND GEOCHEMICAL INVESTIGATIONS OF THE CLASTIC SEDIMENTARY ROCKS OF THE IKORONGO GROUP OF NORTH-WESTERN SERENGETI DISTRICT, NORTH-EASTERN TANZANIA Charles Kasanzu Master of Science (Geology), August 2007 The Neoproterozoic Ikorongo Group, which lies unconformably on the late Archaean Nyanzian Supergroup of the Tanzania Craton, is comprised of conglomerates, quartizites, shales, siltstones, red sandstones with rare flagstones and gritstones. The Ikorongo Group is subdivided into three stratigraphic formations namely the Kinenge, Sumuji and Masati Formations. Two new stratigraphic members for the Sumuji Formation are proposed. The oldest unit is the shale member which comprises of four alternating horizons of brown and green shales containing mudcracks, rain drops and ripple marks and is here reported for the first time to step on basement rocks. The shaly member is overlain by the siltstone member which consists of ferrugenised and cross-laminated micaceous siltstones. The presence of mud cracks and ripple marks in the rocks of the Sumuji Formation signifies deposition in shallow water environments which had experienced intermittent sub-aerial exposure and desiccation. Major element data for the shales and siltstones of the Sumuji Formation indicate broad compositional similarity with both PAAS and NASC. The shales are, however, relatively depleted in CaO and P2O5 compared to PAAS and NASC whereas the siltstones show relative depletion in TiO2, Al2O3, CaO, K2O and P2O5. Compared to both PAAS and NASC, the shales show relative depletion in the trace elements Sr, Zr, Hf, Mo, Nb and Cu whereas Cs, Ba, Ta and Cr are relatively enriched. On the other hand, the siltstones are depleted in the LILE as well as Nb, Mo, Cr, Ni, Sc and V relative to both PAAS and NASC. The chemical index of alteration (CIA: 52 - 82) and the Rb/Sr ratios (0.5–8.6) reveal a moderately weathered protolith. Based on Ni/Al and Mo/Al temporal variation diagrams, oxic conditions predominated during the deposition of the brown shales, anoxic periods prevailed during the deposition of the green shales whereas unstable anoxic-oxic states prevailed during the deposition of the siltstones. The consistent REE patterns, which are characterised by enrichments in the LREE over the HREE ((La/Yb)CN = 7.3-38.3) and negative Eu anomalies (Eu/Eu* = 0.71 on average) whose characteristics are similar to those of PAAS and NASC, illustrate cratonic sources that formed by intra-crustal differentiation. Geochemical considerations and palaeocurrent studies suggest that the provenance of the Ikorongo Group include high-Mg basaltic andesites, dacites, rhyolites and granitoids from the Neoarchaean MusomaMara Greenstone Belt to the north of the Ikorongo basin. Mass balance calculations suggest relative contributions of 50%, 36% and 14% from granitoids, high magnesium basaltic-andesites and dacites respectively to the detritus that formed the shales. Corresponding contributions to the siltstones detritus are 58%, 38% and 4% respectively. The geochemistry of the mudstones suggests that the Neoproterozoic upper crust of the Tanzania Craton had an overall granodioritic composition. The abundances of TiO2, total Fe2O3, MnO, Sc, Cr, Ga, Ge, Sb, Ba, LREE, and Bi are, however, higher than the crustal abundances reported by Taylor and McLennan (1985) and McLennan (2001). On the other hand, the abundances of CaO, Na2O, P2O5, Sr, and Sn are lower than the upper crustal values of Taylor and McLennan (1985) and McLennan (2001). The Neoproterozoic upper crust of the Tanzania Craton had the following elemental ratios: K/U = 10862; K/Rb = 250; Zr/Nb = 10; Zr/Hf = 35; Nb/Th = 1.1; Nb/Ta = 11; Rb/Cs = 30; and Th/U = 3.8. ************** 731 CONAS A MATHEMATICAL MODEL WITH SOURCE OR SINK TERM FOR PREDICTING THE SPREAD OF POLLUTANTS IN LAKE VICTORIA Zubeda Mussa Master of Science (Mathematics), October 2008 Pollution in Lake Victoria at Mwanza South Saanane Island is a major issue which has attracted great attention by many people. Several scientific studies have been carried out in order to understand the depth of the problem and come up with a mitigative solution.In this study we have developed a mathematical model with source or sink term. The model intended to predict the spread of pollutants in the research area. The model was solved using a finite difference technique forward in time and centered in space with appropriate initial and boundary conditions imposed and implemented using Matlab language. Simulations were done when the source or sink term is neglected and then concentration was released as a source to some selected locations and the spreading behavior of the concentration was observed. For comparison, field data was collected and analyzed and similar trends has been obtained between the field measurements and the corresponding numerical prediction. The model is intended for prediction of the spreading nature of pollutants in shallow water areas where the effects of source (discharge) and sink (settling at bottom) are considered. ************** PRICING OF ENERGY BY MEANS OF STOCHASTIC MODEL Emmanuel Evarest Master of Science (Mathematics), October 2008 In this dissertation we present a mean-reverting jump di®usion model for pricing of energy particularly electricity by means of stochastic model. We discuss the stochastic model which is used to model the behaviour of electricity prices. Despite some distributional similarities with asset prices, electricity prices have dramatically di®erent stochastic properties from those of ¯nancial products and even other commodities due to its non-storability nature. These properties include mean-reversion, stochastic volatility, seasonality as well as short lived spikes or jumps. The recent deregulation of electricity markets in the world has exposed power producers and users to market risk due to these unique features of energy price dynamics. The prices contain strong mean reversion, which rejects the demand and supply movements. The model developed is calibrated using the market data from Nordpool for the period from January 1997 to April 2000. The daily price model is estimated via Maximum Likelihood-Conditional Characteristic Function (MLCCF) to obtain the solution in closed form. Then we simulate the model from the parameters obtained, we found that the simulated and real price series have similar trends and covers the same price ranges. From the model we derive the corresponding forward prices under Q-martingale measure and calculate forward 732 University of Dar es Salaam prices at di®erent expiries. All forward prices are subject to the market price of risk due to the fact that power markets are incomplete markets. The ability to model the spot prices and obtain forward price dynamics is essential when assessing the performance of headging strategies that use forward contracts. ************** LIVELIHOOD DIVERSIFICATION AND ITS IMPLICATION ON WETLANDS AND DRYLANDS OF THE LOWER RUFIJI ECOSYSTEMS Mwakipesile Augustino Master of Science (Natural Resources Assessment and Management), August 2008 This study was conducted in the Lower Rufiji Ecosystems in three villages namely Muyuyu, Kikale and Ruaruke. The study aimed at examining the implication of livelihood diversification on dryland and wetland ecosystems. Primary and secondary data were collected using quantitative and qualitative methodology. 5% of households were drawn in each village as a sample size for household interviews. SPSS was used in data analysis. The results of the analysis suggest that agriculture, fishing and forest activities have dominated the livelihood systems. The livelihood systems have been shaped and determined by changing hydrological variables. There have been great variations on the occurrence of floods and rainfall in the lower Rufiji ecosystems overtime which resulted in a shift of cultivation from the floodplain into the terrace ecosystems and into the Rufiji delta mangrove forests. The diversion of Rufiji River fresh water from South into North delta streams has also been a factor behind the shift in cultivation. The changes in hydrology have forced people to start cultivation by clearing miombo, coastal and mangrove forests. Its repercussions were decreases in the forests cover of the miombo, coastal forests and mangrove forests, hence the degradation of the ecosystems’ health and integrity. The study recommends sustainable natural resources management and livelihood strategies to halt this process. Also the study suggests areas for further research including livelihood systems, water resource variability. ************** INVESTIGATION ON CONSERVATION NEED AND BIOACTIVITY OF MEDICINAL PLANTS USED IN THE MANAGEMENT OF HIV/AIDS OPPORTUNISTIC INFECTIONS IN BUKOBA RURAL DISTRICT, TANZANIA Daniel Patrick Kisangau Doctor of Philosophy (Botany) October, 2008 The aim of this thesis was to evaluate the conservation need and efficacy of plants used in traditional medicine to manage Human Immunodeficiency Virus and Acquired Immunodeficiency Syndrome (HIV/ AIDS) opportunistic infections in Bukoba rural district, Tanzania. Ethnobotanical surveys revealed that 75 plant species belonging to 66 genera and 41 families were used to treat one or more HIV/AIDS related 733 CONAS infections in the district. Seven plant species were considered of highest priority for conservation in the district, followed by 10 species of secondary priority. In vitro antimicrobial assay of some crude extracts, semi-purified fractions and pure compounds revealed significant activity against laboratory bacteria and fungi of medical importance. A cytotoxicity test using brine shrimp lethality assay revealed that Pseudospondias microcarpa was the most toxic plant with LC50 (Lethal concentration of a substance that kills 50% of the test organisms) of 1.9 µg/ml (95% Confidence interval, 1.3-2.5 µg/ml), while Zehneria scabra was the least toxic plant with LC50 of 179.4 µg/ml (95% Confidence interval, 156.1-213.9 µg/ml). Chemical analysis of potential plant extracts and semi-purified fractions revealed different chemical compositions. Anticandida efficacy test of plant extracts using in vivo mice infection model portrayed a substantial dose dependency in all treatments made. At a dose of 400 mg/kg, Capparis erythrocarpos was the most effective with mice survival of 60% and organ burden clearance ranging from 64.0%-99.9% (P<0.0001). The study established the potential of traditionally used plants as a source of new drugs for the management of HIV/AIDS opportunistic infections. It also underscores the need to utilize bioactivity data in medicinal plant conservation efforts. ************** LEVELS OF var2csa EXPRESSION IN PLASMODIUM FALCIPARUM PARASITES INFECTING YOUNG TANZANIAN CHILDREN Thomas Felix Marandu Master of Science (Molecular Biology), November 2008 Plasmodium falciparum genome contains a family of 50-60 var genes that code for P. falciparum Erythrocyte Membrane Proteins 1 (PfEMP1). These proteins are expressed at the surface of the infected erythrocytes and they have been implicated in cytoadherence of the infected erythrocytes to various endothelial receptors found in various organs. var2csa is a relatively conserved var gene which codes for VAR2CSA protein. The gene is up-regulated in parasites from the placenta and selected Chondroitin Sulfate A (CSA)-adhering parasites. Recent studies have reported the expression of this gene in parasites infecting children. In this study, the expression levels of var2csa in parasites from children below five years with different clinical presentations of malaria and different binding phenotypes were examined using real time PCR technique. The results show significantly higher var2csa transcript levels in samples that came from children with severe malaria than samples that were from children with non-severe malaria (p<0.05; Mann Whitney U test). These results suggest that var2csa gene expression level is related to severity of malaria in children. There was no significant difference in var2csa transcript abundance between samples that had binding to CSA and samples that had a binding to other receptor molecules (p>0.05; Mann Whitney U test). Specific VAR2CSA human monoclonal antibodies did not react with the infected erythrocytes from children. This might be due to antigenic variation or absence of the protein at the surface of infected erythrocytes from children. The findings from this study suggest that var2csa gene expressed by parasites infecting children can be a good target for vaccines or therapeutics development against severe malaria in children. ************** 734 University of Dar es Salaam ECOTOURISM AND LIVELIHOODS AMONG LIVESTOCK KEEPERS IN THE NGORONGORO CONSERVATION AREA (NCA), TANZANIA Kokel Melubo Master of Science in Natural Resources Assessment and Management , July 2007 This study examines the contribution of ecotourism on improving the social economic wellbeing of livestock keepers in Ngorongoro Conservation Area (NCA), Tanzania. The survey was conducted in seven cultural Bomas situated along the main tourist roads and three luxurious lodges’ on the Ngorongoro crater rim. Cultural bomas are staging centers established for local people to exhibit, demonstrate and sell handicrafts and get photographed by tourists to generate income. Interview of key informants and questionnaire administration to cultural boma occupiers were major techniques of data collection. Direct observations, photography and review of findings from other studies were used to complement the above methods in collecting the required information. The study established that by participating in ecotourism activities individuals have increased the size of the small stocks (goats and sheep) supplied maize flour and hence, paid school contributions and purchased pupils’ uniforms. Other indirect benefits include a wide array of skills, e.g. leaderships, entrepreneurship (such as marketing, handicrafts production) and communication. At community level, secondary and primary schools have been built and renovated, students have been sponsored and water projects established. However, the challenges hindering ecotourism success include poor foreign languages competence, lack of land rights, limited market opportunities and poor participation. It was concluded that in order to maximize socio-economic development without jeopardizing the natural and cultural environments, socio political empowerment should emphasize education and training. ************** ASSESSMENT OF PHYSICO-CHEMICAL PARAMETERS AND THEIR IMPACTS ON THE WATER QUALITY AT IGOMBE RESERVOIR, TABORA, WESTERN TANZANIA Jacqueline Mwendwa Masters of Science (Environmental Sciences), October 2007 Assessment of physico-chemical parameters and their impacts on water quality at Igombe reservoir was done by investigating the influence of anthropogenic activities on water quality during wet and dry seasons. Standard methods were used to determine the levels of physico-chemical parameters from the water samples. Physical parameters including temperature, turbidity, alkalinity, dissolved oxygen (DO), electrical conductivity (EC), and pH, were determined in situ. Chemical parameters such as NO 3-, SiO2, PO43-, Fe2+, SO42-, Cl-, and major cations were determined in the laboratory. Data show higher concentration of physico–chemical parameters during the wet season than during the dry season at various sampling sites. This has been attributed to reservoir-increased input of the physico- chemical parameters by surface 735 CONAS runoff. The mean variation of NO3-, SiO2, PO43-, Fe2+, SO42-, and Cl- concentrations ranged from 0.7 to 2.9 mg/l, 11.38 to 34.9 mg/l, 0.02 to 0.27 mg/l, 0.04 to 0.86 mg/l, 6.3 to 7.8 mg/l, and from 5.2 to 6.5 mg/ l respectively. Data have shown that processes including dissolution, diffusion, adsorption, absorption, nitrification, denitrification, mixing and reduction along with anthropogenic activities explain the variation of physico-chemical parameters in the reservoir. It has been concluded that human activities impacts significantly on the water quality of the reservoir. It is recommended that continuous monitoring and surveillance of anthropogenic activities taking place around the Igombe reservoir should be conducted in order to control pollution from both point and non point sources to ensure sustainable water resource at the reservoir. ************** HUMAN-WILDLIFE CONFLICTS AND OPTIONS FOR INTERVENTIONS IN GAME CONTROLLED AREAS IN TANZANIA A Case of Kilombero Game Controlled Area, Morogoro Amani Gibson Master of Science (Natural Resource Assessment and Management), 2007 This study examined the human-wildlife conflicts and options for interventions in Kilombero Game Controlled Area (KGCA) and suggested measures towards sustainable resource management of the game controlled area. Participatory Rural Appraisal (PRA) methods and questionnaire survey were used to provide information on human-wildlife conflicts, various socio-economic activities and their implications on management of wild animals including their habitats.Findings from this study revealed that human activities in the KGCA have significantly contributed to human-wildlife conflicts. Such a situation has resulted into loss of crops, livestock, properties as well as loss of people’s negative attitudes and some times loss of human lives. Findings further revealed that 59% of human-wildlife conflicts in the KGCA involved farmers and wildlife, while the remaining 41% involved pastoralists and wildlife. Increasing human population and livestock have contributed to expansion of agricultural as well as grazing lands at the expense of wildlife habitats, hence, increased human-wildlife conflicts and decline in wildlife populations in KGCA. As intervention to the conflict situation, farmers have relied on scaring-off the wild animals, using fire for large destructive mammals and dogs for small mammals, particularly primates. To minimize such conflicts, community should be educated and trained in order to build local innovative techniques as well as capacity in human-wildlife conflict resolution. Part of KGCA should be de-gazetted and the remaining part up-graded to Game Reserve status. ************** 736 University of Dar es Salaam ABUNDANCE AND DIVERSITY OF SMALL MAMMALS IN DISTURBED AND UNDISTURBED FORESTS IN THE ULUGURU MOUNTAINS, TANZANIA, Elikana Kalumanga Master of Science (Natural Resource Assessment and Management), 2007 Small mammals are a poorly known fauna in the Eastern Arc Mountains. In order to contribute to the knowledge of this faunal group, small mammals were trapped in the Uluguru Mountains, Tanzania to assess their abundance and diversity in the disturbed and undisturbed forests. The disturbed habitat included the cultivated field (shamba) and the fallow fields while the undisturbed habitat included the intact natural forest (Forest A) and a slightly disturbed forest (Forest B). In each habitat, 3 nested plots were set in order to study their habitat characteristics. Small mammals were caught using Sherman live traps, snap traps and pitfall traps in both dry and wet seasons. A total of 449 individuals, spread over 14 species of rodents and Crocidura shrews (identified to the genus level only) were trapped. The intact forest (A) had the highest diversity of small mammal; the cultivated field recorded the lowest diversity (F3, 14= 0.8049; P = 0.4983). The fallow land recorded the highest trap success (50), followed by Forest A (45.5) and Forest B (42). The least trap success was recorded in the shamba (27.4) (H 0.05, 8, 12, 11, 9 = 24.84, x2 0.05, 3, = 7.815, P= 0.001). All the paired plots scored over 50% similarity indices, suggesting that, within a plot, species composition in all seasons did not vary significantly. Only generalist species were found in both disturbed and undisturbed forests. The forest-specialists (e.g. Beamys hindei) were strictly found in the forests. Canopy avoiding species (e.g. Lophuromys flavopunctatus) were mostly found in the disturbed forests. Therefore, the disturbed forests can not sustainably support diverse small mammal species as it is in the natural forests. ************** MOLECULAR CHARACTERIZATION OF CANDIDA SPECIES ASSOCIATED WITH VULVOVAGINAL CANDIDIASIS IN DAR ES SALAAM, TANZANIA Victor Anacletus Master of Science (Molecular Biology), April 2006 Vulvovaginal candidiasis (VVC) is one of the most common diagnoses made by gynaecologists the world over. Although it is rarely life threatening, the costs of the disease to patients and society are great. Candida albicans is the most common cause of VVC; however other species, more resistant to antifungal drugs, are also involved. When the infecting species is known, it is possible to predict its susceptibility and the information can be used as a guide to therapy. However, identification of vaginal yeasts is based on phenotypic characteristics, which are unreliable and the exercise is laborious. This study identified species of the genus Candida that are associated with VVC in symptomatic women of Dar es Salaam using molecular genetic approaches. Thirty yeast isolates were screened for C. albicans using nested-PCR that targeted two 737 CONAS genes, ERG11 and TOP2. The isolates that were screened out as non-albicans Candida species were speciated using multiplex PCR and PCR-RFLP targeting the 5.8S-ITS region of the ribosomal DNA. The isolate that could not be conclusively identified by any of the above methods, was identified by sequence analysis of the D1/D2 domain of the 26S rDNA. A phylogenetic inference with pathogenic Candida species reported elsewhere was done. C. albicans was the most prevalent (73.3%), followed by C. tropicalis (13.3%), C. glabrata (10%) and C. parapsilosis (3%). Species that cause VVC in Dar es Salaam are closely related to those causing VVC elsewhere in the world. The techniques used in this study were proved to be superior to traditional techniques which base on phenotypic characteristics. Multiplex PCR was the best technique because three different species (8 isolates) were rapidly and correctly identified by this method. For more accurate diagnosis and effective treatment of VVC in Tanzania, molecular genetic approaches empowered with sequence databases that exist for all yeasts described to date, should be used. ************** REHABILITATION OF THE CORAL REEF HABITAT AND ITS EFFECT ON THE RECRUITMENT OF MOTILE INVERTEBRATES AND FISH Magreth Lawrence Mchome Master of Science (Marine Biology), September 2006 This study concerned rehabilitation of the coral reef habitat and its effects on the recruitment of motile invertebrates and fish. The rehabilitation involved transplanting different genera of hard corals only, algae only and a mixture of hard corals, algae and soft corals, cemented onto concrete blocks placed in degraded rubble areas of two patch reefs, Fungu Yasin, Dar es Salaam and Mwambakuni, Bagamoyo. The treatments were arranged in a randomized block design along with controls consisting of blocks only and rubble. Fish were observed for 3-min intervals and recorded by family and size category. Invertebrates were counted. Growth rates of transplanted organisms were determined by periodically measuring heights and widths of canopies/colonies. Recruitment of fish was significantly greater, in terms of abundance, diversity (family) and representation of size classes, on plots rehabilitated with a mixture of organisms than on plots with hard coral only, algae only, blocks only or rubble. It appears that transplanting with a mixture of organisms better replicates the reef habitat and provides a variety of microhabitats and food. There were no consistent differences in motile invertebrate recruitment among treatments. Sea urchins were the most abundant invertebrates. Branching Acropora generally showed faster growth rates than other hard corals, while submassive forms such as Pocillopora, Galaxea and Porites submassive showed intermediate rates and massive forms, particularly Favites, showed the slowest growth. The soft coral transplanted in this experiment showed slow growth in height, but very rapid lateral growth in comparison with hard corals. ************** 738 University of Dar es Salaam ENVIRONMENTAL PREDICTORS OF MATING COMPETITIVENESS IN MALE ANOPHELES GAMBIAE SENSU STRICTO (DIPTERA: CULICIDAE) MOSQUITOES Kija Richard Ng’habi Master of Science (Applied Zoology), May 2007 The enhancement of mating competitiveness of released males carrying the malaria refractory gene to be introduced among wild populations of females of Anopheles gambiae mosquitoes, is critical to malariavector control programmes. Current evidence suggests that genetically engineered males have low mating fitness. As a means to compensate for this reduced fitness, this study was undertaken in an effort to identify environmental conditions that maximize their mating competitiveness and optimise laboratory-rearing regimes. Anopheles gambiae first instar larvae were subjected to three different crowding treatments in the first experiment and three treatments in each experiment were competed against each other for access to females to assess their mating competitiveness. Their long-term survival and teneral energetic reserves were assessed. The low-crowding treatment males were 11 times more competitive than males from high crowding and 3 times more than males from medium crowding treatment. The medium nutritional level males were 6 times more competitive than males from the high nutritional level and 2 times more than those from low nutritional level. Long-term survival and energetic reserves did not influence mating competitiveness. Body size influenced the overall probability of males to secure female mates, but mating competitiveness was dictated by phenotypic similarity of males to the available females. Therefore, releasing males that are phenotypicaly close/similar to the abundant females in the wild would maximize the mating competitiveness of released males. The implications of the results to the new vector control approaches are discussed. ************** GEOCHEMICAL INVESTIGATION OF METAVOLCANIC ROCKS OF THE MUSOMAMARA GREENSTONE BELT IN THE SUGUTI AREA, NORTHERN TANZANIA Mwache Mtoro Master of Science (Geology), August 2007 The Suguti volcanic rocks comprise mainly of a bimodal suite of tholeiitic basalts and calc-alkaline rhyolites with a subordinate amount of intermediate rocks. Zircon U-Pb and whole rock Sm-Nd geochronology, however, reveals that the two suites are cogenetic and were emplaced at 2755 ±1 Ma with a common initial ?Nd value of 2. The tholeiitic basalts are characterised by low abundances of the LILE and HSFE as well as relatively flat chondrite-normalised REE patterns. The rocks also exhibit negative Ti and Nb anomalies in primitive mantle-normalized multi-element diagrams. The flat REE patterns, the presence of prominent negative Nb anomalies and the positive initial ?Nd value of 2 suggest that the basalts were formed in a convergent margin setting by low pressure melting of a mantle wedge which was slightly more enriched than the 739 CONAS MORB source. The enrichment of their source mantle wedge was caused by a moderate to high degree of metasomatism by slab derived fluid as indicated by high Ba/Th ratios (25 - 1492). The calc-alkaline rhyolites are characterised by high abundances of the LILE, moderately high HFSE abundances and very low abundances of the transitional elements. The rhyolites display strongly fractionated, slightly upward concave patterns that are characterized by a slight depletion of the MREE relative to the HREE and moderate to large negative Eu anomalies (Eu/Eu* = 0.3 – 0.9). The depletion of the MREE relative to the HREE is an indication of fractionation of clinopyroxene and hornblende during petrogenesis while the negative Eu anomalies indicate plagioclase fractionation. As isotopic evidence indicates that the basalts and rhyolites form a cogenetic suite with a common initial ?Nd value, the rhyolites are interpreted to have formed from the parental magma of the basalts by fractional crystallization in an active continental margin setting. ************** ABUNDANCE AND SPECIES COMPOSITION OF CRABS AND MOLLUSCS IN DIFFERENT MANGROVE STANDS AT KUNDUCHI, DAR ES SALAAM Julius Elias Daud Master of Science (Environnemental Science), November 2006 The studies of different mangrove stands and their associated fauna are not only fundamental for understanding their ecology but also are important for monitoring, management and conservation of mangrove ecosystems. The influence of different mangrove stands on the abundance and species composition of crabs and molluscs were investigated in the four different stands at the Kunduchi mangrove forest, Tanzania. Four study stands (Avicennia marina, Ceriops tagal, mixed species and cleared stands) were established; where two subareas were set in each stand. A total of 7 crab and 4 molluscan species were recorded during the study period. There was extremely significant difference in total mean fauna abundance among four stands (F (3, 36) = 20.214; p = 0.0001). The higher total mean abundance of 1,134 individuals per m2 was recorded at mixed species, Ceriops being the second with 547 individuals per m2, whereas, the lowest (204 individuals per m2) was found in the Avicennia stand. Likewise, the greatest mean biomass of 36.9 g per 1 m2 for molluscs was recorded in the Ceriops stand, while no molluscs were observed in the cleared stand. The number of taxa, evenness and diversity of crabs and molluscs for each stand were calculated using the Shannon-Weaver index. Ceriops stand supported very significantly greater faunal species diversity of 0.55, followed by 0.34 of mixed stand, and then Avicennia with 0.17, whereas, the cleared stand was having significantly the lowest species diversity of 0.03. Moreover, the mixed mangroves stand supported a greater number of faunal species due to its varied micro-habitats and food availability, while the saplings are thought to be a better food source than those of mature trees. ************** 740 University of Dar es Salaam NUTRIENTS AND PHYTOPLANKTON DYNAMICS IN SELECTED SITES OF RIPARIAN MWANZA, LAKE VICTORIA Salome Daniel Shayo Master of Science (Applied Zoology), June 2007 The study investigated the impact of human activities on nitrogen, phosphorus and silica concentrations and its consequences on phytoplankton abundance, biomass and species diversity in the Southeastern parts of Lake Victoria. Physical chemical variables and biological nitrogen fixation rates were also observed. Sampling was done on a transect from near shore to open waters and also from surface waters to the bottom. The results showed that during the rainy season, Mwanza North Bay and Magu Bay had the highest concentrations of SRP, total nitrogen and dissolved silica at the shoreline stations and highest phytoplankton abundance and chlorophyll a at the open water stations. This could be attributed to runoff from municipal wastes and industrial discharge in Mwanza North Bay and runoff from agricultural areas in the Simiyu River catchments in Magu Bay. Low phytoplankton abundance was also a result of light limitation at the shoreline stations. Similarly, the two sites had lower nitrogen fixation rates and had net fluxes of nutrients towards the sediments. The concentrations of SRP, total nitrogen and silica at Kayenze Bay were about two to three times lower than in Mwanza North Bay and Magu Bay. Furthermore, Kayenze Bay had the highest nitrogen fixation rates. These results signify the importance of in situ regeneration of nutrients at Kayenze Bay possibly due to low input of nitrogen nutrient via runoff from the catchment. Phytoplankton species that were recorded were 131 species of which cyanophyta had the highest diversity and dominancy suggesting nitrogen and phosphorus nutrient enrichment and silica deficiency in Lake Victoria. ************** LITHOLOGICAL AND GEOCHEMICAL CONTROLS FOR GOLD MINERALIZATION IN NYAMULILIMA HILL GOLD DEPOSITS OF THE SUKUMALAND GREENSTONE BELT, NORTH WEST TANZANIA Elias Samson Master of Science (Geology), February 2007 Drill core logging and investigation of rock exposures in Nyamulilima hill gold deposits followed by petrographical and geochemical studies were carried out. The study was aimed to establish lithological and geochemical controls of gold mineralization and hence to clarify the factors that were responsible for gold deposition and distribution in the host rocks. Large part of Nyamulilima hill is covered by metamorphosed interbedded felsic tuffs, quartz-feldspar porphyries and Banded Iron Formation (BIF). Graphitic black shales with pyrite spheroids rarely occur in the area. Granites occur dominantly in the southern and northern parts away from Nyamulilima hill deposits. 741 CONAS The sequence is intruded by younger microgabbros and felsic dykes. Gold mineralization is mostly hosted in silicified, calcite, pyrite-pyrrhotite Banded Iron Formation along the contact with felsic volcanics. Rich ore shoots are associated with quartz-calcite-sulphide veinlets which occur in sheared zones. BIF which hosts high grade gold mineralization is commonly enriched with S, Ag, Sr, V, Cd, Sb and Zn. Barren Banded Iron Formation is either composed of grunerite, quartz, stilpnomelane and magnetite or rich in clay minerals, mainly, kaolinite, chlorite and montmorillonite. Gold in BIF occurs as isolated grains between pyrite and magnetite and is cadmium bearing. Low-grade mineralization is hosted in quartz-feldspar porphyries and felsic volcanics, which are associated with pervasive calcite, disseminated pyrite, sericitization and rare pyrrhotite alterations. Quartrz-feldspar porphyries are often enriched with Ba and Sr. Graphitic black shales and crosscutting microgabbro and felsic dykes are barren. The gold deposits are aligned along roughly NW-SE tectonic lineaments characterized by the presence of isoclinal folds, shear zones, dilatant zones that are regarded to have provided favourable structural traps and permeability essential for gold deposition. The presence of reactive and competent BIF units, which occur adjacent to ductile tuffs are considered to have provided rheological contrast which, was essential for fracturing and subsequently fluid flow. Cadmium gold which is typically associated with magmatic sulphides has been identified in BIF indicating that magmatic processes contributed in ore fluid generation in the Nyamulilima hill deposits. Metamorphic chlorite is commonly overprinted by calcite, quartz, pyrite and pyrrhotite in BIF and interbedded tuffs. The chlorite may have played an important role in provision of Fe which effectively reacted with gold bearing fluids and in turn causing gold deposition. The graphitic black shale seems to have been important in the reduction of ore fluids as evidenced by strong sulphidation and higher gold grades along the contacts of this unit with BIF and felsic volcanics. It is recommended to perform fluid inclusions, isotope studies, dating of granitoids, quartz-feldspar porphyries and gold mineralization and carrying out of Electron Microprobe Analysis (EMPA) on gold crystals in order to further understand gold genesis and factors that controlled its concentration in the Nyamulilima hill gold deposits. ************** THE ROLE OF LOCAL KNOWLEDGE IN THE CONSERVATION OF FOREST BIODIVERSITY IN KILOMBERO DISTRICT, TANZANIA Severin Kusonyola Kalonga Master of Science (Natural Resource Assessment and Management), August 2007 Local Knowledge (LK) in conservation of forest biodiversity is important in ensuring the efficient and most productive use of forest resources. This study, therefore, addresses the role of LK in conservation of forest biodiversity. It aims at informing contemporary approaches to conservation at national as well as local level. The study was conducted in Kilombero District. The methods used were Participatory Rural Appraisal (PRA) techniques, Focus Group Discussion and Questionnaires for data collection. Other methods included participant observation and transect walks. Data was analysed using SPSS and MS Excel. The study identified various traditional practices related to the conservation of forest biodiversity. The study also examined local conservation principles and rules that are in current use. The traditional practices 742 University of Dar es Salaam identified included: setting aside of some forests for the purpose of ritual activities and burial sites; the protection of specific valuable tree species for medicinal purposes, water catchment and ‘homes for spirits’; local allocation of land done by elders who know the values well and the different taboos. The local conservation rules and regulations included, the use of homestead trees and retaining some trees on farmland; the use of coppicing trees and multiple stems for pole production; the use of branches and dead trees for firewood; sustainable harvesting of trees and shrubs for medicines; and the control of wildfire that was normally done by elders. These traditional practices played a significant role in the conservation of forest biodiversity. The role of LK in conservation of forest biodiversity is influenced by population pressure, and the dynamics of globalization, industrialization and urbanization. An effective integration of LK in formal conservation programmes is important. The link between LK and forest biodiversity conservation to poverty alleviation through improvement of livelihoods of the rural people needs to be studied in more detail. The failure to address this link, the unsustainable use of forest resources by local communities will continue at the expense of biodiversity. ************** INVESTIGATION OF THE SEISMO-TECTONIC STRUCTURE OF THE EASTERN BRANCH OF THE EAST AFRICAN RIFT SYSTEM IN TANZANIA Gabriel Daudi Master of Science (Geology), September 2007 To contribute to the understanding of the tectonics of the Tanzanian segment of the eastern branch of the EARS, aeromagnetic, DEM, geological and seismic data were analyzed. The integrated results from these data indicate that spatial distributions of the precise relocated earthquakes are well linked to the structures and lineaments. With that respect, two clusters of activities were obtained one in the Lake Manyara and the second at KwaMtoro. Depth distributions obtained from the relocation and the modelling of magnetic lineaments associated with geological faults range from 5 km to 37 km, with greater depth occurring at KwaMtoro. Results from the distribution of the fault plane solutions used and geological fieldwork in the study indicates two distinct deformation fields: predominantly normal faults in the North Tanzanian Divergence and mainly strike-slip faults at KwaMtoro area. Based on the orientation of the tensional stresses deduced from the fault plane solutions and striation data, three stress regimes have been suggested. The first tensional stress is in the Lake Manyara area within the mobile belt which is oriented WNW-ESE parallel to the regional trend of the EARS. The other two divides the Craton into the northern and southern parts. The northern part is oriented in the NNWSSE tensional stress and the southern part is oriented in the NE-NW tensional stress. Based on the existence of tensional stress orientations within the Tanzania Craton, this study suggests that the extension stress introduced by the EARS to the Craton is accommodated by the two orthogonal oriented stresses. This is observed in the release of energy through both strike-slip and normal faulting in places like Lake Eyasi in the north as well as Balangida depression in the south. ************** 743 CONAS EFFICACY OF SP, SP+ARTESUNATE AND COARTEM® AGAINST FALCIPARUM MALARIA AND FREQUENCY OF MUTANT GENOTYPES IN SOUTH-EAST TANZANIA Siana Nkya Master of Science (Molecular Biology), September 2007 The objective of this study was to assess and compare efficacies of SP, SP+Artesunate and Coartem® as well as frequencies of molecular markers of drug resistance for the first time in Tanzania. A total of 425 children from Lupiro and Kibiti with uncomplicated falciparum malaria, were recruited and treated with either SP, SP+Artesunate or Coartem® then followed up for 28 days. 130 children had treatment failures due to recurrent infections (recrudescence or new infection). Nested PCR-RFLP of msp2 was carried out to discriminate recrudescence from new. Pfmdr1 86 genotypes were analysed by the nested PCR-RFLP technique and SSOP for dhfr/dhps point mutations analysis. Out of the 3 treatment regimens, Coartem® was found to be the most efficacious (92.9%). However, in the Coartem® treatment arm, particularly in the new infections cases, Pfmdr1 86N (wild type) parasites were selected (x 2=7.17, p= 0.007398). Apart from selection being an alert of Coartem® efficacy drawback, this phenomenon represents CQ resistance reversal. Out of 60 and 29 children treated with SP and SP+Artesunate, respectively, parasites with quintuple mutant genotype were the most prevalent ones, with no significant difference before and after recrudescence (SPx2=1.79, p=0.180854) and in SP+Artesunate (x2=0.07, p=0.770816). Generally, the findings of this study have highlighted high Coartem® efficacy in Tanzania underfives. In addition, selection of tolerant parasites following treatment with Coartem® may translate into Coartem® resistance, hence ways to protect the UTL of this drug should commence. On the otherhand, the findings of this study support the present data on SP high resistance which contribute to the failure of SP+Artesunate as well. ************** HEAVY METALS POLLUTION IN WATERS AND SOILS FROM MINING ACTIVITIES IN MERELANI, NORTH-EASTERN TANZANIA Irene Aurelia Tarimo Master of Science (Environmental Science), January, 2007 This study reports on heavy metal pollution in waters and soils from Tanzanite Mines in Merelani, Simanjiro District, Manyara Region, Tanzania. A total of 56 water samples from the study area were collected and analysed both “in situ” and in geochemical laboratory by AAS and HACH machines. A total of 100 mine waste and soil samples were collected from Mines of Blocks B, D and Merelani residential areas and analysed by using AAS machine. The uphill areas without mining activities gave background values for the soil samples. Majengo-KIA residential area, which is 25 km east of the mines gave background values for the water samples. 744 University of Dar es Salaam The results revealed relatively high conductivity in the Mines (7040 µS/cm) and Merelani water samples (1500 µS/cm) compared to WHO (1998) acceptable values of (1000 µS/cm). Total dissolved solids in Mines and Merelani water samples were (5,028 mg/l) and (694 mg/l) respectively, higher than the WHO (1998) which is (500 mg/l). There were high mean concentrations of Cd, Pb, Cr, Fe, Mn and Ni in mine water relative to WHO (1998) acceptable values. Concentration of the principal heavy metals Cd, Cu, Pb and Zn in soil samples showed similar trends as heavy metal concentration in water. The high heavy metal concentration could be from discarded batteries the main source of energy for lighting systems in underground mines. Leaching of metal scraps, explosive remnants, detonating and cabling wires and anthropogenic sources can as well contribute to heavy metal pollution. It is recommended to use rechargeables and solar energy as alternatives to batteries and treat water in areas of elevated heavy metals. In addition, it is suggested to investigate heavy metals wet and dry air deposition and effects on biota, gases concentrations and nutrient load in water sources. Moreover, conduct periodic water monitoring and research other pollution sources of heavy metals. The Merelani community should contain the discarded batteries, explosive remnants, metal scraps, cabling and detonating wires to dispose them as hazardous waste. Education to the Miners and the Merelani community about the effects of environmental pollution and degradation is crucial. There should be routine medical check ups for heavy metal toxicity to the mining communities. ************** THE HYDROCHEMISTRY OF SELECTED TANZANIAN SALT LAKES AND INVESTIGATION OF THE POTENTIAL FOR SALT SEPARATION IN SUCH SYSTEMS Nuru Igumba Kameka Ph.D. (Chemistry), October 2006 This thesis presents the hydrochemistry of salt lakes (Eyasi, Kitangiri, Kindai, Mnangi, Singidani), inflows and upstream sources in the study area and a model for salt separation of brine constituents into pure and marketable form in such systems. The study has revealed that the concentration patterns in the lakes depend on location, depth and season. It has also been shown that in the catchment area, the following electrolyte concentration pattern is discernible: (i) Lake brines> upstream sources (including rivers, boreholes etc). The order of abundance of major ions is: Na+ >> K+ > Ca2 Mg2+: Cl- > (CO32-+HCO3-) > SO42- > PO43- > F-. The solubility equilibrium studies at temperatures 30 oC, 40 oC, 50 oC and 70 oC are presented in terms of phase diagrams for the quaternary systems: (i) NaCl + Na2CO3 + NaHCO3 + H2O (ii) NaCl + Na2SO4 + Na2CO3 + H2O (iii) NaCl + Na2SO4 + NaHCO3 + H2O (iv) Na2CO3 + Na2SO4 + NaHCO3 + H2O. The double salt trona is formed at all studied temperatures in the system NaCl + Na2CO3 + NaHCO3 + H2O while burkeite forms in the system NaCl + Na2CO3 + Na2SO4 + H2O. Both burkeite and trona are formed in the system Na 2CO3 + Na2SO4 + NaHCO3 + H2O. The simulation and optimization of fractional crystallization processes employing the phase rule (phase diagrams) targeting the separation of pure salts from Lakes Balangida, Eyasi and Singidani have been demonstrated. It is found possible to recover all the three salts viz. sodium chloride, sodium sulphate and sodium carbonate in pure marketable forms. ************** 745 CONAS STUDIES ON THE LIFE HISTORY AND ENERGY RESERVES OF MALE ANOPHELES GAMBIAE (DIPTERA: CULICIDAE) FROM KILOMBERO-ULANGA VALLEY Bernadette John Master of Science (Applied Zoology), July 2007 An attempt to develop a method for determining age and mating status of male Anopheles gambiae, was done by observing changes in the features of their reproductive system and these observations were used to explore predictive statistical models for determining age and mating status. The features studied were spermatocysts, sperm reservoir and the clear area. It was noted that the number of spermatocysts in testes decreased with age, and the relative size of the sperm reservoir increased. The presence of a clear area around accessory glands was linked to age and mating status. Of the predictive models explored, the optimal model developed was able to categorize males into age groups of young (4 days) and old (> 4 days) with an overall efficiency of 89%. Based on the parameters of this optimal model, a simple age predicting table was formulated. Mating history could not be reliably assessed based on this approach as virgins could not be distinguished from mated males. Furthermore the age-grading model was adopted on a comparative study of the reproductive potential and life-history of laboratory-reared and wild male An. gambiae s.l.. Three indirect indicators of male mosquito fitness: energetic reserves, body size, and survival were measured in a bid to understand the demographics and energetic limitations of these mosquitoes. The findings established that body size and lipid reserves of wild males were substantially greater than those from standard laboratory conditions. These results demonstrate that measurements of morphological features of male An. gambiae s.l. reproductive system that can easily be collected in the field enables evaluation of life patterns of wild males in free-living populations. ************** HEAVY METAL SPECIATION IN WATER, SEDIMENT AND BIOTA (WATER HYACINTH) FROM MWANZA GULF, LAKE VICTORIA, TANZANIA. Rosemary Bethuel Matemba Master of Science (Environmental Science), February 2007. This thesis reports on heavy metals pollution and speciation in water column, sediments and water hyacinth (Eichhornia crassipes) from Mwanza gulf, Lake Victoria. Samples were analysed for cadmium, cobalt, chromium, iron and lead by Atomic Absorption Spectrophotometry (AAS). In water samples, the mean concentrations of Cd and Pb have been found to vary significantly between dry and wet seasons, as well as among the sampling stations. The highest concentrations of cadmium (0.77 ± 0.008 ppm), iron (0.82 ± 0.003 ppm) and lead (2.8 ± 0.17 ppm) were found at Mirongo station within Mwanza urban area. Cobalt and chromium were not detected in all the water samples. The heavy metals analysed in water samples existed mainly as total labile metals. In sediment samples, the highest concentrations of cadmium (0.44 ± 746 University of Dar es Salaam 0.03 ppm), cobalt (0.66 ± 0.01 ppm) and lead (3.80 ± 0.2 ppm) were recorded at stations that were adjacent to river mouths. The highest concentration of Iron (1320.60 ± 0.7 ppm) was recorded at sampling station close to the fish landing beach. Chromium was not detected in all the sediment samples. The heavy metals detected in sediments (Cd, Co, Fe and Pb) were found to exist as exchangeable metals. In water hyacinth, the highest concentration of iron (126.00 ± 0.62ppm) was recorded at Mirongo station. Statistical tests show significant differences in mean concentrations between dry and wet seasons and among the sampling stations. All the heavy metals analyzed in plants existed as labile/soluble metals. ************** THE GENETIC DIVERSITY AND PATHOGENICITY OF RICE YELLOW MOTTLE VIRUS IN TANZANIA Emmanuel Sangu Master of Science (Botany), October 2007 Tanzania is severely affected by Rice yellow mottle virus (RYMV). But, little information is known on the distribution, genetic diversity and pathogenicity of local strains of RYMV. Fifty three isolates collected from selected regions in Tanzania were characterized both serologically and molecularly then the pathogenicity of selected isolates was compared. Three RYMV strains S4, S5 and S6 were confirmed. An overall strain diversity was high with nucleotide divergence of 14.8%. The genetic diversity was exceptionally high in the eastern Tanzania () = 6.1% and decreased westward to () =1.8%. Isolates of strain S4 were recorded for the first time in the Eastern Arc Mountain region (EAM) and they were closely related to L. Malawi strain S4 isolates. Pathogenicity of RYMV isolates was largely cultivar dependant, There was a positive correlation (r = 0.78) between symptom intensity and yield loss. Eight virulent isolates broke the resistance found in Gigante and Tog5681. These isolates had a single substitution mutation at position 48 of the VPg sequence (changes from arg to glu). The findings have reinforced the proposition that EAM region is the centre of origin of RYMV and brings forth a clear view of dispersion of the strains from its centre of origin. The results also underlines the potential risk associated with the effectiveness of genetic control of rice yellow mottle virus based solely on the use of high resistance to RYMV. Farmers should be well informed of the different means of virus transmission because most of the massive infections occur following crop transplantation, weeding and irrigations. ************** IMPACT OF FOREST CONSERVATION PROGRAMME ON THE HABITATS AND OCCURRENCE OF MAMMALS IN KAHAMA DISTRICT Adam Seif Bashemela Masters of Science (Environnemental Science), October 2007 747 CONAS This study on the impact of forest conservation programme on the habitats and occurrence of mammals in Kahama district was done from March to April 2006. Three sites with different conservation strategies were sampled: Community conserved forest (Ngitili), Mkweni Forest Reserve and a Village Forest. The vegetation was sampled using the Modified-Whittaker nested quadrat method, small mammals by trap line method and large mammals by a questionnaire survey and direct observation. Tree species richness of 29 species and a density of 228 trees ha-1 in the Community conserved forest were significantly higher than 12 species and density of 60 trees ha-1 recorded in Mkweni Forest Reserve respectively. Only saplings were found in the Village forest. Small mammals species richness between the three study sites were not significantly different but trap success of 2.18% in the Community conserved forest was significantly higher than that for Mkweni Forest Reserve (0.66%) and the Village forest (1.66%). The species richness of medium to large sized mammals of 21 species in the Community conserved forest was significantly higher than that in Mkweni Forest Reserve and the Village forest with 9 species each. It is concluded that the Community conserved forest had dense vegetation cover and limited human disturbance due to its good conservation programme than the other sites that were highly degraded due to poor management. Sustainable management of the forests and establishment of private ngitili strategy is recommended in order to allow regeneration of the forests and attract more mammal species if properly protected. ************** ASSESSMENT OF ECOLOGICAL VIABILITY OF UPPER KITETE-SELELA MIGRATORY CORRIDOR IN THE TARANGIRE-MANYARA ECOSYSTEM: IMPLICATIONS TO AFRICAN ELEPHANT AND BUFFALO MOVEMENTS Johannah, L.M. Master of Science (Natural Resource Assessment and Management) (NARAM), 2007 Ecological viability of the Upper Kitete-Selela wildlife migratory corridor was assessed in Lositete, Upper Kitete and Selela Villages, specifically its size, habitat, human threats and the extent of its use by african elephants and buffaloes. The corridor is one of the few remaining but threatened corridors connecting NCA, LMNP and Mto-wa-Mbu GCA. Aerial photographs of January 1958 and a LANDSAT imagery of December, 2002 were used to establish habitat change. Extent of corridor-use by elephants was determined through counting elephant dung-piles along line transects and analyzed through use of a DISTANCE 4.1 programme. SPSS program was used to analyze data from questionnaire-based interviews. The habitat in the study area has significantly changed and corridor’s size has been reduced to an average width of 1.5km, a length of 3.3km and an area of 8.0km2. Open grassland has decreased from 2348ha in 1958 to 636ha in 2002 while dense bushland has decreased from 259ha in 1958 to 86ha in 2002. These changes have been due to increased human settlements and cultivation. Elephants’ use of the wide corridor’s stratum (2.0 km) was significantly higher in wet season than in the narrow stratum (0.9 km) during dry season (Z=-4.2, p=0.0001) due to different habitat requirements, widths and extent of human disturbances between the strata. They mostly use the corridor after it is dark due to human disturbances. Thus, the corridor is relatively viable ecologically and it is highly threatened by humans. Hence, human pressure and human-wildlife conflict control, and land use plans are recommended. **************
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