Specifications Agilent 1200 Series Isocratic Pump
Flow
Precision
≤0.07 % RSD,or ≤0.02 min SD whatever is greater, based on
retention time at constant room temperature
Flow accuracy
± 1 % or 10 µL/min whatever is greater
Range
Setpoints from 0.001 to 10.0 mL/min,
in 0.001 mL/min increments
Pressure
Operating range
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi) up to 5 mL/min
0 - 20 MPa (0 - 200 bar, 0 - 2950 psi) up to 10 mL/min
Pulsation
<2 % amplitude (typically <1 %) at 1 mL/min isopropanol at all
pressures >1 MPa (> 10 bar, >147 psi)
Compressibility compensation
User selectable, based on mobile phase compressibility
Recommended pH range
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for
continuous tracking of instrument usage, display of feedback
messages if preset limits are exceeded.
An optimized design ensures virtually pulse-free and stable solvent flow, with dual floating pistons in series,
precisely servo-controlled. This delivery principle allows variable stroke volume optimized to selected flow
rate. An inlet-valve electronically activated and synchronized to piston stroke - eliminates vapor formation
with volatile solvent and is maintenance free.
Features and Benefits
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Ease-of-use - the Agilent 1200 Series instant pilot allows you to enter operating parameters fast,
store methods easily and display pressure profile, diagnostics and task-oriented information.
Robust materials withstand most demanding applications - stainless steel, titanium, gold, ruby,
sapphire, ceramics, PEEK and PTFE ensure long lifetime. Seal wash option for high salt mobile
phases keeps maintenance to a minimum for lower operation costs.
Versatile wide flow range - up to 10 mL/min for broad HPLC work, including semipreparative
separations.
More help for GLP - built-in leak test with graphical presentation of pressure profiles, and early
maintenance feedback.
Low operating and maintenance costs - due to long instrument uptime.
Future proof - upgradeable to quaternary pum
Specifications Agilent 1200 Series Binary Pump
Flow
Flow precision
<0.07 % RSD,or ≤0.02 min SD whatever is greater, based on retention
time at constant room temperature
Flow accuracy
± 1 % or 10 µL/min whatever is greater
Range
Setpoints from 0.001 to 5.0 ml/min, in 0.001 ml/min increments
Pressure
Operating range
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi)
Pulsation
<2 % amplitude (typically <1 %) at 1 ml/min isopropanol at all pressures >1
MPa (> 10 bar, >147 psi)
Compressibility compensation
User selectable, based on mobile phase compressibility
Gradient
Delay volume
180 - 480 µl (600-900 µl with mixer) dependent on back pressure
Composition precision
<0.15 % RSD, 1 mL/min
Composition accuracy
<0.5 % absolute
Recommended pH range
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for continuous tracking of instrument
usage, display of feedback messages if preset limits are exceeded
High pressure mixing for microbore and standard columns
Whether operating standalone with the Agilent 1200 Series instant pilotor as part of an Agilent
ChemStation-controlled HPLC system, the Agilent 1200 Series binary pump is designed for top
performance high-pressure mixing. It is the pump of choice for reproducible gradients and high performance,
especially at low flow rates and for high throughput applications. The Agilent 1200 Series binary pump has
an optional programmable solvent selection valve which combines two out of four solvents for utmost
flexibility and increased convenience, for example, when flushing the column.
Features and Benefits
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Low internal and external volumes of capillary connections and a minimum of bench space needed
due to two dual-piston, in-series pumps in one housing.
Meeting highest chromatographic demands by using servo-controlled pistons of both individual
pumps.
A wide flow range starting at typically 50 µL/min for reproducible gradients at low flow with solvent
degassing.
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Reliable design keeping maintenance to a minimum using electronically-controlled active inletvalves for flow rates up to 5.0 mL/min. the binary pump is also ideally suited to do standard-bore
chromatography. Floating pistons with path-of-least-resistance principle ensures highest seal
lifetime.
Specifications Agilent 1200 Series Quaternary Pump
Flow
Flow precision
≤0.07 % RSD,or ≤0.02 min SD whatever is greater, based on retention time
at constant room temperature
Flow accuracy
± 1 % or 10 µL/min whatever is greater
Range
Setpoints from 0.001 to 10.0 ml/min, in 0.001 ml/min increments
Pressure
Operating range
0 - 40 MPa (0 - 400 bar, 0 - 5880 psi) up to 5 ml/min
0 - 20 MPa (0 - 200 bar, 0 - 2950 psi) up to 10 ml/min
Pulsation
<2 % amplitude (typically <1 %) at 1 mL/min isopropanol at all pressures
>1 MPa (> 10 bar, >147 psi)
Compressibility compensation
User selectable, based on mobile phase compressibility
Gradient
Delay volume
800 - 1100 µL depending on back pressure
Composition precision
<0.20 % SD, at 0.2 and 1 mL/min
Recommended pH range
1.0 - 12.5
Solvents with pH <2.3 should not contain acids which attack steel
GLP features
Electronic records of maintenance and errors.
Early maintenance feedback (EMF) for
continuous tracking of instrument usage, display of feedback messages if
preset limits are exceeded.
Excellent low pressure mixing of up to four
solvents
The optimized design of the Agilent 1200 Series quaternary
pump with incorporated vacuum degasser ensures virtually
pulse-free and stable solvent flow, with dual floating pistons in
series, precisely servo-controlled. This design with variable
stroke volume allows pulse-free solvent delivery and efficient
mixing. An inlet-valve electronically activated and synchronized
to piston stroke - eliminates vapor formation with volatile solvents
and is maintenance free. The Agilent 1200 Series quaternary
pump provides highest flexibility in solvent mixing and is
recommended for a wide range of research and routine
applications, and in the method development area.
Features and Benefits
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Speeding up method development, preparation of mobile phases and flushing the HPLC system by
offering convenient access of up to four solvents for isocratic or gradient analysis.
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Keeping maintenance to a minimum for lower operation costs by using robust materials such as
stainless steel, titanium, gold, ruby, sapphire, ceramics, PEEK and PTFE that withstanding most
demanding applications ensuring long lifetime. The optional active seal wash option is
recommended for high salt mobile phases to prevent corrosion.
A wide flow range of up to 10 mL/min for broad HPLC work, including semipreparative separations.
Easy programming and control through the handheld controller, instant pilot, or through different
Agilent data systems, e.g. the Agilent ChemStation.
More help for GLP using the built-in leak and pressure test with graphical presentation of pressure
profiles, and early maintenance feedback.
Performance Specifications 1200 Series standard autosampler
Valid when standard 100 µL metering head installed
Injection range
0.1- 100 µL in 0.1 µL increments
Up to 1500 µL with multiple draw (hardware modification required)
Precision
<0.25% RSD from 5-100 µL, <1% from 1-5 µL, variable volume
Sample viscosity range
0.2 - 5 cp
Sample capacity
100 × 2-ml vials in 1 tray
40 × 2-ml vials in ½ tray
15 × 6-ml vials in ½ tray (Agilent vials only)
Injection cycle time
Typically 50 s depending on draw speed and injection volume
Carry-over
Typically < 0.1 %, < 0.05 % with external needle cleaning
Can be upgraded from non-thermostatted version to thermostatted version by adding the Agilent 1200
Series thermostat for ALS/FC/spotter
Features
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Reliable injections from 0.1 µL to 100 µL
Easy adaption for injection volumes up to 1500 µL for applications ranging from microbore to
semipreparative chromatography
Low internal volume of 300 µL for the minimum contribution to a system's total internal volume can
be even further reduced using "by-pass" mode
Overlapped injections for increased productivity
Sample capacity
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100 × 2-mL vials in a standard tray
40 × 2-mL vials in a standard tray
15 × 6-mL vials in a half tray
Microvials (100 or 300 µL) with sleeves
Thermostatting
Peltier temperature control from 4 to 40 °C for up to 100 2-mL vials containing
thermally-labile samples
Specifications 1200 Series Variable Wavelength Detector
Detection type
Double-beam photometer
Light source
Deuterium lamp
Noise
± 0.75 x 10 -5 AU, at 254 nm
Drift
3 x 10-4AU/hr, at 254 nm
Linearity
>2 AU upper limit
Wavelength range
190-600 nm
Wavelength accuracy
± 1 nm, self-calibration with deuterium lines, verification with holmium
oxide filter
Wavelength bandwidth
6.5 nm typical over whole wavelength range
Maximum data rate
13 Hz
Flow cells
Standard: 14-µL volume, 10-mm cell path length and 40 bar (588 psi)
pressure maximum
High Pressure: 14-µL volume, 10-mm cell path length and 400 bar (5880
psi) Pressure maximum
Micro: 1-µL volume, 5-mm cell path length and 40 bar (588 psi) pressure
maximum
Semimicro: 5-µL volume, 6-mm cell path length and 40 bar (588 psi)
pressure maximum
Communications
Controller-area network (CAN), RS-232. APG Remote:ready, start, stop
and shut-down signals, LAN optional
Safety and maintenance
Extensive diagnostics, error detection and display through control module
and Agilent ChemStation, leak detection, safe leak handling, leak output
signal for shutdown of pumping system. Low voltages in major
maintenance areas.
GLP
Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback
messages if preset limits are exceeded. Verification of wavelength
accuracy with built-in holmium oxide filter
Sensitive and stable UV-visible detection
Whether as a standalone detector extending your analytical
range, or in an automated Agilent 1200 Series system enhancing
your productivity, the Agilent 1200 Series variable wavelength
detector (VWD) lowers detection limits with simplified optics for
high sensitivity, low baseline drift and wide linear range. Lower
your cost of ownership with easy front access to lamp and flow
cell for fast replacement. Helps to comply to GLP with built-in
holmium oxide filter for automatic wavelength verification.
Features and Benefits
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Achieve high sensitivity and low detection limits with low baseline noise and drift.
Cut operating costs with automatic shutdown for longer productive lamp lifetime.
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Verify wavelength accuracy for GLP compliance - automatic holmium oxide filter can be set at the
beginning of your chromatography to verify that your wavelength is indeed what the setpoint says it
is.
Realtime chromatographic signal plot and built-in context-sensitive online information with easy
step-by-step guidance on handheld control modules (Agilent 1200 Series handheld control module
or the new Agilent 1200 Series Instant Pilot).
Highest uptime through simplified design.
Specifications Agilent 1200 Series Multiple Wavelength Detector
Detector type
1024-element diode array
Light source
Deuterium and tungsten lamp
Short-term noise
± 0.8 x 10 -5 AU at 254 nm and at 750 nm
Drift
0.9 x 10 -3 mAU/hr, at 254 nm
Linearity
>2 AU upper limit
Wavelength range
190-950 nm
Wavelength accuracy
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide
filter
Slit width
Programmable: 1, 2, 4, 8, 16 nm
Diode width
< 1 nm
Wavelength bunching
Programmable, 2 - 400 nm, in steps of 1 nm
Flow cells
Standard
13-µL volume, 10 mm-cell path length, 120 bar (1760 psi) pressure maximum
Semi-micro
5-µL volume, 6-mm cell path length, 120 bar (1760 psi) pressure maximum
Micro
2-µL, volume, 3-mm cell path length, 400 bar (5880 psi) pressure maximum
Semi-nano
500-nanoliter volume., 10-mm cell path length, 50 bar (735 psi) pressure
maximum
Nano
80-nanoliter volume, 6-mm cell path length, 50 bar (735 psi) pressure
maximum
High pressure
1.7-µL volume, 6-mm cell path length, 400 bar (5880 psi) pressure maximum
Preparative
3-mm cell path length, 120 bar (1760 psi) pressur maximum
0.3-mm cell path lenght, 20 bar (294 psi) pressure maximum, quartz
0.06-mm cell path length, 20 bar (294 psi) pressure maximum, quartz
Time programmable
Wavelength, polarity, peak width, lamp bandwidth, autobalance, wavelength
range, threshold, spectra storage mode
Analog output
Recorder/integrator 100 mV or 1 V, 2 outputs
Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback
messages if preset limits are exceeded. Verification of wavelength accuracy
with built-in holmium oxide filter
More information and flexibility without any loss in
sensitivity
The Agilent 1200 Series multiple wavelength detector allows a
wide range of wavelengths from low UV to the visible range. The
diode array based dual lamp design provides high sensitivity for
trace-level quantification. Simultaneously compounds of interest
can be quantified and impurities are monitored at additional
wavelengths. It is recommended for the pharmaceutical QA/QC
laboratory, bioscience applications (proteins, DNA) and as a
general purpose UV detector for routine work The temperature
management system ensures optimum baseline stability–even
under rough ambient conditions.
Features and Benefits
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Excellent signal-to-noise – for lowest limits of detection
Up to five signals – enables highest selectivity without loss of sensitivity
Extended wavelength range – enables a wide range of applications from low UV to the visible
range maintaining a low noise level
Tools for conventient GLP – for example, built-in wavelength calibration and automated OQ/PV
procedures
Upgradeable to full spectral capabilities – to protect your investment today
Low cost of ownership - long life deuterium lamp (>2000 h), graphical ChemStation user interface to
speed up training time, built-in diagnostics software, easy front access to lamp and flow cell for fast
replacement.
Specifications Agilent 1200 Series Diode-Array Detector
Detector type
1024-element diode array
Light source
Deuterium and tungsten lamp
Short-term noise
± 0.8 x 10 -5 AU at 254 nm and at 750 nm
Drift
0.9 x 10 -3mAU/hr, at 254 nm
Linearity
>2 AU upper limit
Wavelength range
190-950 nm
Wavelength accuracy
± 1 nm, self-calibration with deuterium lines, verification with holmium oxide
filter
Slit width
Programmable: 1, 2, 4, 8, 16 nm
Diode width
< 1 nm
Wavelength bunching
Programmable, 2 - 400 nm, in steps of 1 nm
Flow cells
Standard
13-µl volume, 10 mm cell path length, 120 bar (1760 psi) pressure maximum
Semi-micro
5-µl volume, 6 mm cell path length, 120 bar (1760 psi) pressure maximum
Micro
2-µl volume, 3 mm cell path length, 400 bar (5880 psi) pressure maximum
Semi-nano
500-nanoliter volume 10-mm cell path length, 50 bar (735 psi) pressure
maximum
Nano
80 nanoliter volume., 6-mm cell path length, 50 bar (735 psi) pressure
maximum
Preparative
3-mm cell path length, 120 bar (1760 psi) pressur maximum
0.3-mm cell path lenght, 20 bar (294 psi) pressure maximum, quartz
0.06-mm cell path length, 20 bar (294 psi) pressure maximum, quartz
High pressure
1.7-µl volume, 6-mm cell path length, 400 bar (5880 psi) pressure maximum
Time programmable
Wavelength, polarity, peak width, lamp bandwidth, autobalance,
WL range, threshold, spectra storage mode
Spectral tools
Data analysis software for spectra evaluation, including spectral libraries and
peak purity functions
Analog output
Recorder/integrator 100 mV or 1 V, 2 outputs
Electronic records of maintenance and errors. Early maintenance feedback
(EMF) for continuous tracking of instrument usage, display of feedback
messages if preset limits are exceeded. Verification of wavelength accuracy
with built-in holmium oxide filter
For complete confidence from spectral data
Add spectral acquisition to your HPLC analyses with the worldwide leading Agilent 1200 Series diode-array detector. Verify
separation quality with peak purity, multiple signals and spectral
libraries - interactively or automatically in the same run as your
analytical quantification. Extended wavelength range from 190 to
950 nm with highest sensitivity or spectral resolutionwith
programmable slit. And holmium oxide wavelength verification for
GLP compliance. The temperature management system ensures
optimum baseline stability–even under rough ambient conditions.
Access the spectral domain with superior 3D optics–
simultaenous illumination using combined deuterium and tungsten lamps for highest intensity and lowest
detection limit from 190 to 950 nm, 1024 diodes and 1-nm slit for highest spectral resolution.
Programmable slit helps you explore the spectral landscape for faster sample characterization–focus
on fine bands with narrow setting, or if higher sensitivity is required open up the programmable slit for more
light throughput. A broad slit ensures lowest baseline noise and highest chromatographic signal-to-noise.
Store and report slit settings, from 1 - 16 nm, together with your raw data for GLP traceability.
Convenient spectral storage and flexible viewing –easily overlaid spectra within a run or between runs.
Verify wavelength accuracy for GLP compliance–automatic holmium oxide filter (which can be set at the
beginning of your chromatography) verifies that your wavelength is indeed what the setpoint says it is.
Connect to LC-MS–high pressure micro flow cell for extremely small internal volumes are required for LCMS applications and use a single Agilent ChemStation to acquire and view UV and MS spectral data.
Explore capillary HPLC– flow rates as low as 5-µl/min with the new 500-nanoliter flow cell. Increase
sensitivity 50 to 100-fold compared to standard-bore HPLC.
Low cost of ownership
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Graphical Agilent ChemStation user-interface speeds up training time
Long life deuterium lamp (>2000 h)
Built-in diagnostics software
Easy front access to lamp and flow cell for fast replacement