Create a more natural cell environment using Transwell® Permeable Supports 1. Select a Membrane PC (Polycarbonate) PET (Polyester) PTFE (Polytetrafluoroethylene) Translucent membrane suitable for a range of applications; high pore density allows maximum diffusion Transparent membrane suitable for visualization of cell outlines by phase contrast microscopy Collagen-coated PTFE membrane which promotes cell attachment and allows cells to be visualized 2. Select a Pore Size Application Cell Type Pore Size (µm) Angiogenesis Endothelial, HMVEC, HUVEC 3.0 Co-culture Stem, neuronal and various others 0.4, 1.0 Epithelial Cell Polarity Epithelial cells 0.4 Migration Endothelial cells, HUVEC, HMVEC Neutrophils, PMNs Lymphocytes, macrophages, monocytes Neuronal cells Dendritic cells Neurite outgrowth Epithelial fibroblasts, Smooth muscle Leukocytes 3.0 3.0 3.0, 5.0 3.0 3.0, 5.0, 8.0 1.0, 3.0 8.0 3.0, 5.0 Invasion Melanoma, Glioma Lymphoma, Jurkat Osteoblasts Breast cancer Endothelial 8.0 5.0, 8.0 8.0 5.0, 8.0 3.0, 5.0, 8.0 Smaller pore sizes (0.4 and 1.0 µm) are used for culturing cells, co-culture applications and drug transport studies Larger pore sizes (3.0 to 8.0 µm) are recommended for chemotaxis and angiogenesis applications Tissue Engineering Human skin model 0.4, 3.0 Toxicity Testing Mouse fibroblasts Human lung 3.0 0.4 Transport and Caco-2 Permeability Studies MDCK 0.4, 1.0 0.4, 1.0 3. Select a Format Individual inserts for 6-, 12-, or 24- HTS insert plates for automation Snapwell inserts for use in well plates or 100 mm dishes and ease of handling diffusion or Ussing chambers Netwell inserts for culture of small organs and tissue slices 4. Select a Surface Treatment Tissue culture (TC) Transwell-COL For optimal cell attachment and growth PC and PET membranes Collagen I/III-coated PTFE membrane that promotes cell attachment and spreading Ordering Information Uncoated Individual Inserts Transwell® Permeable Supports, Polycarbonate (PC) Membrane Cat. No. 3412 3414 3428 3401 3402 3413 3415 3421 3422 3419 3420 Description Inserts in 6-well plates Inserts in 6-well plates Inserts in 6-well plates Inserts in 12-well plates Inserts in 12-well plates Inserts in 24-well plates Inserts in 24-well plates Inserts in 24-well plates Inserts in 24-well plates Inserts in 100 mm dish Inserts in 100 mm dish Membrane Pore Size (μm) 0.4 3.0 8.0 0.4 3.0 0.4 3.0 5.0 8.0 0.4 3.0 Qty/Pk 6/plate 6/plate 6/plate 12/plate 12/plate 12/plate* 12/plate* 12/plate* 12/plate* 1/dish 1/dish Qty/Cs 24 24 24 48 48 48 48 48 48 12 12 6/plate 6/plate 12/plate 12/plate 12/plate* 12/plate* 12/plate* 24 24 48 48 48 48 48 6 6 24 24 12/plate 12/plate 6/plate 6/plate 48 48 48 48 *6.5 mm membrane diameter are packaged 12 inserts in a 24 well plate, 4 plates per case. Transwell-Clear Inserts, Polyester (PET) membrane 3450 3452 3460 3462 3470 3472 3464 Inserts in 6-well plates Inserts in 6-well plates Inserts in 12-well plates Inserts in 12-well plates Inserts in 24-well plates Inserts in 24-well plates Inserts in 24-well plates 0.4 3.0 0.4 3.0 0.4 3.0 8.0 *6.5 mm membrane diameter are packaged 12 inserts in a 24-well plate. 4 plates per case. Snapwell™ Inserts** 3407 3801 PC inserts in 6-well plates Clear PET inserts in 6-well plates 0.4 0.4 **Diffusion chambers are available through Harvard Apparatus (www.harvardapparatus.com). Netwell® Inserts, PET Membrane 3477 3478 3479 3480 Inserts in 12-well plates Inserts in 12-well plates Inserts in 6-well plates Inserts in 6-well plates 74 440 74 440 Netwell Accessories Cat. No. 3517 3519 3520 3521 Description Netwell reagent tray, black Netwell reagent tray, white Netwell 12-well carrier kit for 15 mm inserts Netwell 6-well carrier kit, for 24 mm inserts Qty/Cs 200 200 8 8 Coated Individual Inserts Transwell®-COL Collagen-coated Inserts** Cat. No. 3491 3492 3493 3494 3495* 3496* Description Inserts and 6-well plates Inserts and 6-well plates Inserts and 12-well plates Inserts and 12-well plates Inserts and 24-well plates Inserts and 24-well plates Membrane Pore Size (μm) Oty/Pk 0.4 1 3.0 1 0.4 1 3.0 1 0.4 1 3.0 1 Oty/Cs 24 24 24 24 24 24 *Includes inserts packaged separately with multiwell plates. * *6.5 mm diameter inserts packaged separately with two 24-well plates. HTS Insert Plates HTS Transwell-24 Well Permeable Supports 3396 3397 3398 3399 3395 3378 3379 HTS Transwell-24, individual, polycarbonate (PC) HTS Transwell-24, bulk, PC HTS Transwell-24, individual, PC HTS Transwell-24, bulk, PC HTS Transwell nontreated reservoir HTS Transwell-24, bulk, PET HTS Transwell-24, individual, PET 0.4 0.4 3.0 3.0 0.4 0.4 1 12 1 12 12 12 1 2 12 2 12 48 12 2 0.4 0.4 0.4 1.0 1.0 3.0 3.0 5.0 5.0 8.0 8.0 - 1 1 5 1 1 1 4 4 1 1 4 10 10 10 10 1 5 5 1 5 2 8 8 2 2 8 10 10 10 10 HTS Transwell-96 Well Permeable Supports 3381 3391 7369 3380 3392 3385 3386 3387 3388 3374 3384 3382 3383 3583 3783 HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC HTS Transwell-96 System reservoir and receiver plates with 2 lids, PET HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PET HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PET HTS Transwell-96 well plate, receiver plate and lid, individual, PC HTS Transwell-96 well plate, receiver plate and lid, bulk, PC HTS Transwell-96 well plate, receiver plate and lid, bulk, PC HTS Transwell-96 well plate, receiver plate and lid, individual, PC HTS Transwell-96 well plate, receiver plate and lid, individual, PET HTS Transwell-96 well plate, receiver plate and lid, bulk, PET HTS Transwell-96 receiver plate with lid, standard tissue culture-treated HTS Transwell-96 reservoir plate media stabilizer and lid HTS Transwell-96 black receiver plate with lid, standard tissue culture-treated HTS Transwell-96 white receiver plate and lid, standard tissue culture-treated NOTE: The enclosed samples are non-sterile For additional product information, visit www.corning.com/lifesciences Corning Incorporated Life Sciences 836 North St. Building 300, Suite 3401 Tewksbury, MA 01876 t 800.492.1110 t 978.442.2200 f 978.442.2476 www.corning.com/lifesciences A S I A / P AC I F I C China t 86 21 3338 4338 f 86 21 3338 4300 Japan t 81 3-3586 1996 f 81 3-3586 1291 Singapore t 65 6572-9740 f 65 6861-2913 Australia/New Zealand t 61 427286832 Taiwan t 886 2-2716-0338 f 886 2-2516-7500 Korea t 82 2-796-9500 f 82 2-796-9300 India t 91 124 4604000 f 91 124 4604099
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