Create a more natural cell environment using Transwell

Create a more natural cell
environment using
Transwell® Permeable Supports
1. Select a Membrane
PC (Polycarbonate)
PET (Polyester)
PTFE (Polytetrafluoroethylene)
Translucent membrane suitable for
a range of applications; high pore
density allows maximum diffusion
Transparent membrane suitable
for visualization of cell outlines by
phase contrast microscopy
Collagen-coated PTFE membrane
which promotes cell attachment
and allows cells to be visualized
2. Select a Pore Size
Application
Cell Type
Pore Size (µm)
Angiogenesis
Endothelial, HMVEC, HUVEC
3.0
Co-culture
Stem, neuronal and various others
0.4, 1.0
Epithelial Cell Polarity Epithelial cells
0.4
Migration
Endothelial cells, HUVEC, HMVEC
Neutrophils, PMNs
Lymphocytes, macrophages, monocytes
Neuronal cells
Dendritic cells
Neurite outgrowth
Epithelial fibroblasts, Smooth muscle
Leukocytes
3.0
3.0
3.0, 5.0
3.0
3.0, 5.0, 8.0
1.0, 3.0
8.0
3.0, 5.0
Invasion
Melanoma, Glioma
Lymphoma, Jurkat
Osteoblasts
Breast cancer
Endothelial
8.0
5.0, 8.0
8.0
5.0, 8.0
3.0, 5.0, 8.0
Smaller pore sizes (0.4 and 1.0 µm)
are used for culturing cells, co-culture
applications and drug transport
studies
Larger pore sizes (3.0 to 8.0 µm) are
recommended for chemotaxis and
angiogenesis applications
Tissue Engineering
Human skin model
0.4, 3.0
Toxicity Testing
Mouse fibroblasts
Human lung
3.0
0.4
Transport and
Caco-2
Permeability Studies MDCK
0.4, 1.0
0.4, 1.0
3. Select a Format
Individual inserts for 6-, 12-, or 24- HTS insert plates for automation Snapwell inserts for use in
well plates or 100 mm dishes
and ease of handling
diffusion or Ussing chambers
Netwell inserts for culture of
small organs and tissue slices
4. Select a Surface Treatment
Tissue culture (TC)
Transwell-COL
For optimal cell attachment and growth
PC and PET membranes
Collagen I/III-coated PTFE membrane that promotes cell attachment
and spreading
Ordering Information
Uncoated Individual Inserts
Transwell® Permeable Supports, Polycarbonate (PC) Membrane
Cat. No.
3412
3414
3428
3401
3402
3413
3415
3421
3422
3419
3420
Description
Inserts in 6-well plates
Inserts in 6-well plates
Inserts in 6-well plates
Inserts in 12-well plates
Inserts in 12-well plates
Inserts in 24-well plates
Inserts in 24-well plates
Inserts in 24-well plates
Inserts in 24-well plates
Inserts in 100 mm dish
Inserts in 100 mm dish
Membrane
Pore Size (μm)
0.4
3.0
8.0
0.4
3.0
0.4
3.0
5.0
8.0
0.4
3.0
Qty/Pk
6/plate
6/plate
6/plate
12/plate
12/plate
12/plate*
12/plate*
12/plate*
12/plate*
1/dish
1/dish
Qty/Cs
24
24
24
48
48
48
48
48
48
12
12
6/plate
6/plate
12/plate
12/plate
12/plate*
12/plate*
12/plate*
24
24
48
48
48
48
48
6
6
24
24
12/plate
12/plate
6/plate
6/plate
48
48
48
48
*6.5 mm membrane diameter are packaged 12 inserts in a 24 well plate, 4 plates per case.
Transwell-Clear Inserts, Polyester (PET) membrane
3450
3452
3460
3462
3470
3472
3464
Inserts in 6-well plates
Inserts in 6-well plates
Inserts in 12-well plates
Inserts in 12-well plates
Inserts in 24-well plates
Inserts in 24-well plates
Inserts in 24-well plates
0.4
3.0
0.4
3.0
0.4
3.0
8.0
*6.5 mm membrane diameter are packaged 12 inserts in a 24-well plate. 4 plates per case.
Snapwell™ Inserts**
3407
3801
PC inserts in 6-well plates
Clear PET inserts in 6-well plates
0.4
0.4
**Diffusion chambers are available through Harvard Apparatus (www.harvardapparatus.com).
Netwell® Inserts, PET Membrane
3477
3478
3479
3480
Inserts in 12-well plates
Inserts in 12-well plates
Inserts in 6-well plates
Inserts in 6-well plates
74
440
74
440
Netwell Accessories
Cat. No.
3517
3519
3520
3521
Description
Netwell reagent tray, black
Netwell reagent tray, white
Netwell 12-well carrier kit for 15 mm inserts
Netwell 6-well carrier kit, for 24 mm inserts
Qty/Cs
200
200
8
8
Coated Individual Inserts
Transwell®-COL Collagen-coated Inserts**
Cat. No.
3491
3492
3493
3494
3495*
3496*
Description
Inserts and 6-well plates
Inserts and 6-well plates
Inserts and 12-well plates
Inserts and 12-well plates
Inserts and 24-well plates
Inserts and 24-well plates
Membrane
Pore Size (μm) Oty/Pk
0.4
1
3.0
1
0.4
1
3.0
1
0.4
1
3.0
1
Oty/Cs
24
24
24
24
24
24
*Includes inserts packaged separately with multiwell plates.
* *6.5 mm diameter inserts packaged separately with two 24-well plates.
HTS Insert Plates
HTS Transwell-24 Well Permeable Supports
3396
3397
3398
3399
3395
3378
3379
HTS Transwell-24, individual, polycarbonate (PC)
HTS Transwell-24, bulk, PC
HTS Transwell-24, individual, PC
HTS Transwell-24, bulk, PC
HTS Transwell nontreated reservoir
HTS Transwell-24, bulk, PET
HTS Transwell-24, individual, PET
0.4
0.4
3.0
3.0
0.4
0.4
1
12
1
12
12
12
1
2
12
2
12
48
12
2
0.4
0.4
0.4
1.0
1.0
3.0
3.0
5.0
5.0
8.0
8.0
-
1
1
5
1
1
1
4
4
1
1
4
10
10
10
10
1
5
5
1
5
2
8
8
2
2
8
10
10
10
10
HTS Transwell-96 Well Permeable Supports
3381
3391
7369
3380
3392
3385
3386
3387
3388
3374
3384
3382
3383
3583
3783
HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC
HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PC
HTS Transwell-96 System reservoir and receiver plates with 2 lids, PET
HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PET
HTS Transwell-96 system, reservoir and receiver plates with 2 lids, PET
HTS Transwell-96 well plate, receiver plate and lid, individual, PC
HTS Transwell-96 well plate, receiver plate and lid, bulk, PC
HTS Transwell-96 well plate, receiver plate and lid, bulk, PC
HTS Transwell-96 well plate, receiver plate and lid, individual, PC
HTS Transwell-96 well plate, receiver plate and lid, individual, PET
HTS Transwell-96 well plate, receiver plate and lid, bulk, PET
HTS Transwell-96 receiver plate with lid, standard tissue culture-treated
HTS Transwell-96 reservoir plate media stabilizer and lid
HTS Transwell-96 black receiver plate with lid, standard tissue culture-treated
HTS Transwell-96 white receiver plate and lid, standard tissue culture-treated
NOTE: The enclosed samples are non-sterile
For additional product information, visit www.corning.com/lifesciences
Corning Incorporated
Life Sciences
836 North St.
Building 300, Suite 3401
Tewksbury, MA 01876
t 800.492.1110
t 978.442.2200
f 978.442.2476
www.corning.com/lifesciences
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