BD LSRFortessa (2-Blue, 6-Violet, 4-Yellow Green, 3-UV and 3-Red Configuration)
Alexa 488
FITC
GFP
mBanana
Sytox Green
CFSE
YFP
Fluo3AM
PE-Cy5
PE-DY647
PE-Alexa 647
7AAD
BV 711
eFluor 700NC
Qdot 705
BV 605
BV 570
eFluor 605NC
Qdot 605
DsRed
PE
Alexa 546
Alexa 555
Cy3
Alexa Fluor 405
Cascade Blue
CFP
Pacific Blue
BV421
V450
efluor 450
MarineBlue (10%)
VioBlue
Violet Laser
(405nm)
Blue Laser
(488nm)
Yellow Green
Laser (561nm)
PE-Alexa 594
PE-Alexa 610
PE-mCherry
PE-Texas Red
PE-CF594
mitotracker
PI
Texas Red
Alexa 568
Alexa 594
ECD
mCherry
Qdot605 (6%)
Qdot625(2%)
Qdot800 (10%)
PE-Cy7
PE-Vio 770
PE-Alexa 750
Flow Cytometry Core Platform
PerCp
PerCp-Cy5.5
PE-Alexa 680
PerCp-eFluor710
PE-Alexa700
PE-Cy5.5
QDot705(11%)
BV 737
BV 395
Indo-1(Violet)
UV Laser
(355nm)
Alexa Fluor 350
DAPI
Hoechst 33258
Indo-1(Blue)
Hoechst 33342
Marina Blue
Live/Dead fixable blue dye (Aqua)
BV 785
Qdot 800
Amcyan
V500
Qdot 525
Krome Orange
VioGreen
BV510
eFluor 490NC
Alexa Fluor 430
Pacific Orange
Qdot 565
Cascade Yellow
Lucifer Yellow
BV570
Aqua
BV 650
eFluor 605NC
Qdot 655
APC-eFluor780
APC-Alexa750
APC-Vio 770
APC-C750
APC-Cy7
APC-H7
Qdot 525(4%) and Qdot545
eFluor 670
eFluor 660
Alexa 633
Alexa 647
APC
Cy5
Red Laser
(640nm)
Alexa Fluor 680
Alexa Fluor 700
APC-Alexa 680
APC-Alexa 700
Updated 16 Jun 2014
BD LSRFortessa - 10 Jun 2014
Laser
UV 355nm
Violet 405nm
Blue 488nm
Yellow-Green
561nm
Red 640nm
Filters and Mirrors
Band/Pass
Long Pass
Detector
A
740/35
505
BV 737
Colors detectable
B
450 / 50
410
Alexa Fluor 350, DAPI, Hoechst 33258, Hoechst 33342,Indo-1(Blue), Aqua
C
379 / 28
NIL
BV 395, Indo-1(Violet)
A
780/60
750
BV785, Qdot800
B
710 / 50
685
BV711, eFluor 700NC, Qdot 705
C
670 / 30
630
BV655, eFlour 650NC, Qdots655
D
610 / 20
600
BV605, , BV570, eFlour 605NC, Qdots605
E
525 / 50
505
Amcyan, V500,Qdots 525, Krome Orange, VioGreen, BV510, eFluor490NC,Alexa
Fluor 430, Pacific Orange, Qdot565, Cascade Yellow, Lucifer Yellow, BV570
F
450 / 50
NIL
Alexa Fluor 405, Cascade Blue, CFP, Pacific Blue, BV421, V450, eFluor 450, Marine
Blue (minimally), VioBlue
A
685/35
635
B
530 / 30
505
PerCp, PerCp-Cy5.5, PE-Alexa 680, PerCP-eFluor710, PE-Alexa 700, PE-Cy5.5,
Qdot705(11%)
Alexa 499, FITC, GFP, mBanana, Sytox Green, CSFE, YFP, Fluo3AM
A
780 / 60
750
Qdot800(10%), PE-Cy7, PE-Vio770, PE-Alexa 750
B
670 / 30
635
PE-Cy5, PE-DY647, PE-Alexa647, 7AAD
C
610 / 20
600
PE-Alex594, PE-Alexa610, PE-mCherry, PE-Texas Red, PECF594, mitotracker Red, PI,
Texas Red, Alexa568, Alexa594, ECD, mCherry, Qdot605(6%), Qdot625(2%)
D
586 / 15
NIL
DS-Red, PE, Alexa546, Alexa 555, Cy3
A
780 / 60
750
APC-eFluor780, APC-Alexa750, APC-Vio 770, APC-C750, APC-Cy7, APC-H7
B
730 / 45
690
Alexa Fluor 680, Alexa Fluor 700, APC-Alexa 680, APC-Alexa 700
C
670 / 14
NIL
Qdot525(4%), Qdot545, eFluor 670, eFluor 660, Alexa 633, Alexa 647, APC , Cy5