Supplementary Table ST4: Result of BLASTN of LSU rRNA test dataset derived from 43 species against LSUrdb (see very column for species name).
LSUrdp
Cellular
organisms
18/8600
Domain
Bacteria
6382/6400
99.7%
Color coding:
100%
<100%-95%
<95%-90%
<90%-80%
<80%-70%
<70%
Phylum
Proteobacteria
2779/2800
99.3%
Spirochaetes 197/200 = 98.5%
Cyanobacteria 184/200 = 92%
Firmicutes 597/600 = 99.5%
Eukaryota
1190/1200
99.2%
Order
Rhizobiales 359/400 =89.8%
Family
Bradyrhizobiaceae
Beta 563/600 = 93.8%
Burkholderiales 170/200 = 85%
Nitrosomonadales 284/400 = 71%
Burkholderiaceae
Nitrosomonadaceae
Gamma 1381/1400 = 98.6%
Enterobacteriales 681/800 = 85.1%
Enterobacteriaceae
Pseudomonadales 307/400 =76.8%
Pseudomonadaceae
Moraxacellaceae
Chromatiaceae
Myxococcaceae
Helicobacteraceae
Spirochaetaceae
Nostocaceae
Bacillaceae
Clostridiaceae
Mycoplasmataceae
Aquificaceae
Parachlamydiaceae
Bacteroidaceae
Flavobacteriaceae
Chlorobiaceae
Delta 191/200 = 95.5%
Epsilon 200/200 = 100%
Spirochaetes 197/200 = 98.5%
Chromatiales 187/200 = 93.5%
Myxococcales 191/200 = 95.5%
Campylobacterales 200/200 =100%
Spirochaetales 197/200 = 98.5%
Nostocales 171/200 = 85.5%
Bacillales 192/200 = 96%
Clostridiales 183/200 = 91.5%
Mycoplasmatales 200/200 = 100%
Aquificales 200/200 = 100%
Chlamydiales 199/200 = 99.5%
Bacteroidales 188/200 = 94%
Flavobacteriales 189/200 = 94.5%
Chlorobiales 197/200 = 98.5%
Chlorobi 197/200 = 98.5
Chloroflexi 200/200 = 100%
Deinococcus-Thermus =98.5%
Planctomycetes 400/400=100
Bacilli
Clostridia
Mollicutes
Aquificae
Chlamydiae
Bacteroidetes
Flavobacteria
Chlorobia
Dehalococcidetes
Deinococci
Planctomycetacia
Actinobacteria 400/400 =100%
Actinobacteria
Actinomycetales 398/400 = 99.5%
Acidobacteria 194/200 =97%
Thermotogae 197/200=98.5%
Euryarchaeota 400/400=100%
Solibacteres
Thermotogae
Methanomicrobia 194/200= 97%
Halobacteria 200/200 = 100%
Thermoprotei 200/200 = 100%
GroupI.1a 197/200 = 98.5%
GroupI.1b 199/200 = 99.5%
Spermatophyta 350/400 =87.5%
Solibacterales 186/200 = 93%
Thermotogales 197/200=98.5%
Methanosarcinales 194/200 = 97%
Halobacteriales 200/200 = 100%
Sulfolobales 200/200 = 100%
Aquificae 200/200 = 100%
Chlamydiae 199/200 = 99.5%
Bacteroidetes 394/400 =98.5%
Archaea
1000/1000
100%
Class
Alpha 388/400 = 97%
Crenarchaeota 600/600=100%
Viridiplantae 375/400 = 93.8%
Fungi 187/200 = 93.5%
Alveolata 186/200 = 93%
Metazoa 386/400 = 96.5%
Ascomycota 158/200 = 79%
Apicomplexa 186/200 = 93%
Arthropoda 188/200 = 94%
Nematoda 195/200 = 97.5%
Deinococcales 197/200 = 98.5%
Planctomycetales 400/400 = 100%
Poales 75/200 = 37.5%
Brassicales 68/200 = 34%
Saccharomycetales 144/200 = 72%
Haemosporida 185/200 = 92.5%
Diptera 188/200 = 94%
Rhabditida 194/200 = 97%
Deinococcaceae
Planctomycetaceae
Unclass Planctom.
Mycobacteriaceae
Streptomycetaceae
Solibacteraceae
Thermotogaceae
Methanosarcinaceae
Halobacteriaceae
Sulfolobaceae
Poaceae
Brassicaceae
Saccharomycetaceae
Drosophilidae
Rhabditidea
Genus
Bradyrhizobium
Nitrobacter
Burkholderia
Nitrosomonas
Nitrosospira
Salmonella
Shigella
Yersinia
Escherichia
Pseudomonas
Acinetobacter
Nitrosococcus
Myxococcus
Wolinella
Borrelia
Anabaena
Bacillus
Clostridium
Mycoplasma
Aquifex
Cand. Protochlamydia
Bacteroides
Flavobacterium
Chlorobaculum
Dehalococcoides
Deinococcus
Rhodopirellula
Candidatus Kuenenia
Mycobacterium
Streptomyces
Solibacter
Thermotoga
Methanosarcina
Haloquadratum
Sulfolobus
Cenarchaeum
Oryza
Arabidopsis
Saccharomyces
Plasmodium
Drosophila
Cenorhabditis
Species
B. japonicum
N. winogradskyi
B. cepaia
N. eutropha
N. multiformis
S. typhimurium
S. dysenteriae
Y. pestis
E. coli K12
P. fluorescens
A. baumannii
N. oceani
M. xanthus
W.succinogenes
B. burgdorferi
A. variabilis
B. anthracis
C. tetani
M. genitalum
A. aeolicus
P. amoebophila
B. thetaiomicron
F. johnsoniae
Chl. tepidum
D. sp. CBDB1
D. geothermalis
R. baltica
K. stuttgartiensis
M. leprae
S. coelicolor
S. usitatus
T. maritima
M. mazei
H. walsbyi
S. solfataricus
C. symbiosum
Unc. Cren. 54d9
O. sativa
A. thaliana
S. cerevisiae
P. falciparum
D. melanogaster
C. elegans
The data set consisted of 8,600 simulated ribo-tags (200 randomly generated ones from each species). The ribo-tags were taxonomically affiliated according to a
BLASTN-bit score of 86, and BLASTN hits within the top ten percent of Bit score were included in the taxonomic analysis with MEGAN. The number and
percentage of ribo-tags for each species are given at different taxonomic resolution, from the domain until the order level. The color coding refers to different
percentages of ribo-tags correctly affiliated at a given taxonomic level. No ribo-tag was falsely-assigned. 18 ribo-tags were affiliated as "cellular organisms", i.e.
could not be assigned to the correct domain of live.