L-selectin controls trafficking of chronic lymphocytic
leukemia cells in lymph node high endothelial venules in
vivo
by Fanny Lafouresse, Elisabeth Bellard, Camille Laurent, Christine Moussion, JeanJacques Fournié, Loïc Ysebaert, and Jean-Philippe Girard
Blood
Volume 126(11):1336-1345
September 10, 2015
©2015 by American Society of Hematology
Analysis of the HEV network in CLL/SLL LNs. (A) Representative images showing the density
and the distribution of HEV blood vessels (MECA-79+, green) in a reactive LN (control; left) and a
CLL LN (CLL; right).
Fanny Lafouresse et al. Blood 2015;126:1336-1345
©2015 by American Society of Hematology
CD20+ CLL cells accumulate within HEV pockets in CLL LNs. Four-color confocal images
showing the accumulation of CD20+ CLL cells (light blue) within HEV pockets (arrowheads) in
CLL LNs. HEV endothelial cells were stained with MECA-79 (green) and CD31 or von...
Fanny Lafouresse et al. Blood 2015;126:1336-1345
©2015 by American Society of Hematology
CLL cells roll, stick, and crawl within LN HEVs in vivo.
Fanny Lafouresse et al. Blood 2015;126:1336-1345
©2015 by American Society of Hematology
L-selectin is the key factor controlling the arrest of CLL cells within LN HEVs. (A) Flow cytometry
analysis of the expression of L-selectin, LFA-1, and CXCR4 on CD19+CD5+ CLL cells.
Fanny Lafouresse et al. Blood 2015;126:1336-1345
©2015 by American Society of Hematology
Idelalisib treatment is associated with decreased expression of L-selectin on circulating CLL
cells in vivo.
Fanny Lafouresse et al. Blood 2015;126:1336-1345
©2015 by American Society of Hematology