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Oxygen
Various
By
Consumption
of Erythrocytes
Thyroid
Conditions
Related
Serum
Protein-Bound
Iodine
LUIS
ANGELONE,
I
HAS
been
metabolism
adrenalectomy
a return
to
produces
limits
sumption
to
demonstrated
is comparable
causes
about
normal
followimsg
a decrease
in
the
of
dosages
and
;4
increase
in
AND
iii
;
vitro,
to
-
give
that
that
ats
of whole
“protect”
against,
or
aging
its vitro
roentgen
within
the
their
oxygen
‘on-
in
adretsal
cortical
to
metabolism
quantitative
extract
prior
the induced
amstagotsize,
studies
oms variations
beets made
oms isucleated
its the comscemstratiots
erythrocytes,
e.g.,
of thyroid
s’hole l)lood
mso experimetstal
of knowms
respective
data
comscehitratiohs
on
the
study
was umsdertakems,
comicentrations
on the
of mson-msucleated
erythrocytes,
amid second
of
the respiratory
metabolism
of these
comscentratiotss
of circulatimsg
thyroid
hormomse,
serum
proteims-bound
iodine
(PBI)
values.
,
t o attempt
erythrocytes
as expressed
correlatiots
in terms
by their
(storage)
rays,
imscrease
of alligators6
and red cells of ducks,7
to our kmsow’ledge
exist
to show
the effect
of variatiomis
its thyroid
hormone
respiratiots
of nomi-nucleated
red cells of any animal.
This
first,
to determine
the effect
of various
thyroid
hormone
a
AN;EREIs
metabolism.5
Although
respiratory
hormone
in vivo have
respiratory
A.
CLIFFORD
red (‘ells that
their
respiratory
msormal
resting
tissues;’
that
in their
oxygen
uptake
w’ith
therapy;2
metabolism
admitsistratioms
appears
their
appropriate
their
the
WATKINS,
for nomi-miucleated
to that
of other
73 per cent decreases
employed
that
x-irradiatioms
H.
I)AvID
from
Patients
with
to Their
Respective
Concentrations
METHODS
Patients
School
of both
were
sexes
into four
divided
disturbances
frons
(euthyroid),
(B)
the
Ohio
groups
State
University
as follows:
untreated
hypert
treated
with ten drops
of Lugol’s
solutions
days (not the same patients
as used in group
The
on
clinical
determination
of these
(A)
four
Hospital
l)atients
hyrois!
three
(B),
groups
and
with
patients,
(C)
times
daily
for
and (D) untreated
was
based
the
no
Columbus
indications
State
of thyroid
hyperthyroid
patients
fourteens
to
hypothyroid
prinsarily,
h)Ut
not
twenty-one
patients.
conclusively,
their
serum PBI concentrations
which
tend
to (‘orrelate
with determinationis
of basal
metabolic
rates
and with cliniical
evidences
of the
state
of thyroid
fundtiohi.8
The serum
PBI was determined
as described
by Connor,
Curtis
ansi Sw-enison,28
and a ramige of 3.7 to
6.7 zg. per cenut was considered
miornsal, i.e., euthyroid!.
With
heparin
as ani aisticoagulant,
30 to 50 cc. of blood were witholrawn
initravenouslv
from each patient.
No attempt
was made to place the patient
under
basal condil ions before
taking
a blood
(50 per
scribed.2
to
cent
sansple.
Two
and
of dry
Frons
the
wt.)
cc.
for
study
of each
red
Departments
red
for
one-half
six respirometers
gram
The
suspensions)
of
cells were
respiratory
sansples
at
37.5
The
cell suspension
Pisysiology
red
cell
(cu. nim.
Qo2
was
and
Krebs-phosphate-Rinsger
according
of a given
C.
in
prepared
studies
bases!
Surgical
State University,
Columbus.
This investigation
was aided by the Comly-Colensani
on
to
the
suspension
02
the
solutioni
technic
were
nsean
placed
Ier
consumed
Research,
previously
value
College
into
ho)ur
of hue
of
per
dethree
milli-
nunsber
Medicine,
of
Thue
Ohio
The
Ohio
Submitted
State
Research
Fund,
College
University.
September
14,
1953;
accepted
for
953
publication
March
1, 1954.
of Medicine,
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954
OXYGEN
respironseters
used
to
CONSUMPTION
represent
it at
OF
any
given
ERYTHRO(’YTES
All
time.
respironseters
frequency
of 80 cycles
per minsute
and
at an amplitude
The respirations
of hiormal
red cell suspensions
was
meoliatelv
technsic
after
(2 to 8 C.).
preparatiots
In agreement
between
mean
after
technic
withdrawal
was used
tainied
anidl
frons
with
values
Q(I2
of
was
blood
or
various
the
other
mifter
studs’
The
data
are
stamsdard
sumption
were
compared
groups
shows
show
<
0.20)
from
1 the
is suggested
msatural
serum
1.-Summary
(P
Patient
for
PBI values
% difference
red
of
logarithms
SE.
the
the
Erythrocyte
Various
*
A
and
=
t Three
Qo2
and
the
indicated
values
the
are
Respective
Serum
PBI
B
C
D
0.025
0.016
0.011
+92
+23
-15
8
11
0.031
0.010
0.20
0.012
0.004
0.50
1.2
-73
5
10
0.833
0.263
0.01
1.606
0.05
C =
related
Indicated
3.603
0.225
iodine-treated!
hyperthyroid
and
patients.
to six respiromet
PBI
(D)
decrease
.4
+91
-
Serum
of
12
hyperthyroid,
values
aisd y the red cell Qo.
of the form y = ae,
0.014
0.005
0.04
4.4
untreated
Qo.
ceist
from
8
0.781
B =
with
hypothyroid
15 per
Qo.
Groups
12
0.003
0.001
hypothyroid
and
various
-
%)
values
the mean
a
x the PBI
by a curve
-
euthvroid,
ob-
respective
serum
PBI values
to these
data,
a linear
relatioms
A
patients
untreated
cells
significant.
Thyroid-Patient
P-values
D =
meats
cell suspemssiomss
of the
Callimsg
are fitted
(±)
()
S.D.
Warhurg
of red
for the oxygen
conred cell suspensioiss
(C)
patients’
curve
groups’
(,g.
the
but
the
When
and
0.013
Mean
immediately
the untreated
hyperthyroid
(B)
which
is considered
statistically
0.50)
statistically
% difference
Number
of patienitst
S.D. ()
SE.
(±)
l’-value
Number
<
not
Qo2 values
Meanu
im-
difference
either
Eventually
shown
groups.
various
PBI values.
the data
of
Taiues
technic
refrigerations
significant
redi cells
hyperthyroid
are
the
of
respectively,
measurements
are
thyroid
are plot-ted
relative
to the
Fitting
the best probable
the
Fenn
made
its vitro.
(A) group,
(P < 0.04),
imscrease
which
whets
TABLE
various
cent
values
respective
the
overnight
were
of normal
1 . Here
iodine-treated
per
Qo2
groups
per cent.
their
the
the
a 23
at
ESULTS
in table
respectively,
In figure
thyroid
its jig.
the
whereas
groups
24 hours
onily
by
after
(S.D.)
atsd statsdard
errors
(S.E.)
serum
PBI
values
of the respective
with
the euthyroid
a 92 per cent increase
significant;
to
and
prepared
are
(P
summarized
deviatiomss
(Qo2)
which
for
not
oscillated
groups.
H
their
studies!
deternsinationis
storage
were
ens.
no statistically
#{176}. ‘#{176}
whether
for the
thyroid
Warllurg
workers,3
tiotedi,
exclusively
the
by
of 3.9
nc
determimiations
determinations
for
per
iodine-treated
patient.
hyperthyroid!
patients
are
without
meaning.
§ S.D.
ability)
means
=
value
to the
standard
is obtaitied
estimated
deviatioti
arid
SE.
=
statsdard
from t-lal)le,
where I
standard
error of this
is the
error
ratio
difference.
of the
of the
indicated
nsean.
o!ifferenice
between
P (probsamples
a
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ANGELONE,
2
WATKINS,
4
AND
6
955
ANGEIIER
8
PROTEiN-BOUND
IODINE
,UGM.%)
FIG.
1 -Oxygen
consumptioni
(Qc2) of erythnocyte
suspenusionus
l)lo)ttesl
relative
to I hseir
respective
serum
proteins-bound
iodine
values
(.ug. per cent)
of various
thyroid
pat ienuts.
The patient
groups
are idientified
as follows:
0 = euthyroid!,
#{149}
= hypothyroid!,
= huvperthyroid,
and ‘- inc!icates
the nseani Qo, value
(ord!inate)
for the iodine-treated
huyperthyroid
group.
Of course
in the last group
a mean
serum
PHI value
(abscissa)
is wit how.
meaning.
pH 7.4; temperature
37.5 C.
where
b
This
and
a
logarithms.
the
respective
are
(otsstants
to be determihied
problem
was domse by fitting
Qo,
lated
to their
the
by
measuremeists
error
of y about
this
its exponeistial
form
arsd e
a straight
method
the
line
is
of least
squares.
curve
is fi4#{149}*
The resultihsg
curve
(fig. 1) where
Qo. values
of the red
corresponsding
serum
Pill
values
its g.
base of the huattmral
to the logarithms
of
The
statidard
then
translated
cell suspetssioms
are
to
re-
‘as
per
cent.
DIscUSSIoN
From
rocyte
previous
observatiotss,’3
suspemssiomss
is due
primarily
reported
here,
night
*
cold
We
EJniversity,
wish
in
oxygen
storage
has
to
for
thank
his
readly
h
to
comssumption
beets
Prof.
D.
s’ooperat
observed
Ransoms
ions
it appears
‘
imitact
of
msoms-msucleated
by other
Whit
that- the
(‘ells. The
niey,
i ni fit hi ng
Statistical
1 o I lie
of erythdecrease,
after
erythrocytes
itsvestigators
a curve
respiration
negligible
for nucleated
Laboratory,
dat
a unuder
The
Oluio
(‘onsiderat
overerythSlate
ions.
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956
OXYGEN
rocytes.9
for
trating
of these
the
of
oxygen
the
impossil)le.
OF
great
of red
How’ever,
(p. 358).
Our Qo.
the former
two.
has
It
beets
adequately
or hypothyroid
sumptioms
Pomsder25
per
from
cent)
toward
tendency
statistically
that
o2
values
roid
group.
the
111
than
However,
less; the
This
Assuming
with
group.
It
has
hormomseh7
results
h8
added
ots
thyroid
is
tso
apparetst
effect
tsor
on
the
respiratory
to the
statsdard
0.003,
thyroid
imscrease
(92
show
omsly a
This
latter
value
its
control
on the
abscissa
basis that this
asymptotically.
0.833,
Direct
beets
the
A).
portioms
It is
5 (3 cretins)
gave
and
the other
6 (3 cretimss)
of the meats
for the euthy-
there
are
two
levels
of activity
probably,
that
some factor
other
its adremsocortical
fumsction.h4,
hyperthyroid
(B)
thets
1.6 g.
group
treatmetit
are
of
the
15
per
cent
or
due
to
the
hyperthyroid
=
the
actioms
of the
metabolism
of isolated
circulating
tissues
from
oms
tissues
its vitro,’9
iodimse
small
treated
daily
it is utilikely
that
the
per
variable
of
C
may
meats
be
the
Q.
meats
values
its
oms the
Se.
deviations
0.014,
thyroid
the rat,
effect
group
is due to iodine
doses
of thyroxits
cause
of skeletal
muscles
from
amsimal
to
treatmemst
result
its more
umsiform
results.
its question.
of
is rsot
(group
cotssumptioms
amid lomsger
disturbances
glaisds
deviatioms
yet
B
the
eryth-
(Lugol’s)
solutioms,
which
is kmsown to depress
thyroid
might
depress
the Qo. values
for erythrocyt.es
from
the
apparent
from
the data that this occurs.
Simice iodimse adminis-
directly
large
roid
perhaps
con-
The
sigmiificamit
patients
with
value
that
half
oxygen
ioditse
sults
its the oxygen
whereas
larger
doses
=
amsd liver.ts
of 1 1 patients,
or, more
imbalance
hormotse,
respiration
of red cells of the
beets reported
for dogs2#{176}
that
(A
that
patients
e.g., an
group
hyperthyroid
kidmsey,
Qo.,.
favorably
either
its the
its meats
euthyroid
one
the meats
approximately
made
compared
to
to suggest
the
formatioms,t6
tration
the
cent)
whets
(D)
tetids
coiscen-
attempted
compares
respectively,
a similar
hypothyroid
from
hypothyroid
circulatioms
group
hormomse
whets
per
0.20)
hypothyroid
is critical,
that
of
latter
show
subject,
the serum
PBI values
its all cases,
except
one, were
msormal values
range
from 3.7 to 6.7 g. per cent.8
amoumst
(C)
its this
so-called
the thyroid
<
atsimals
iserve,
those
of the
patietsts
of significance
is explainable
(fig. 1) is approachihsg
the
comparable
which
were
values
euthyroid
muscle,
(15
(P
The cause
of this lack
of the Q02-PBI
curve
gave
e.g.,
a decrease
and
oxygen
cotisumptioms
of
in terms
of Qo.. After makto be 0.015,
0.017,
0.042
arid
foutsd
or decrease,
whereas
sigtsificaist
interestitsg
for
patiemits
respiration,
(staisdardizing)
of preparimsg
for
reports
were
that
ats imscrease
hyperthyroid
its
values
earlier
of 0.013
tissues,
expressing
atsd
its a review
the
demotistrated
show
of various
rocytes
value
of
cells
studies,
ams absolutely
quamititative
comparison
erythrocytes
with those
foumid its the literature
to recalculate,
amid thus
to stahidardize,
humati
erythroeytes
as found
in four
imsg certaimi
assumptions,
these
values
0.06
with
ERYTHROCYTES
variability
(‘otisumptiohi
red cells for respiratory
results
for noms-msucleated
pra(’tically
is
Because
‘#{176}
. 12
‘
values
CONSUMPTION
the
It has
re-
amsimal,
Thus,
four
groups
0.012,
D = 0.031)
of patients
with
various
due to the differemst
fumsctiomsal
states
of the thy-
=
This
appears
serum
PBI
of the
thyroid
to
he
values
borne
out
the
same
for
by
the
large
groups
stamidard
(0.781,
3.603,
respectively).
imsvolvememst
demonstrated.’3
Simsce
the
hormotse
noms-msucleated
its an
emszyme
erythrocyte
system
may
has
utilize
msot as
glu-
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ANGELONE,
and
cose
lactic
system25’
apparently
membrane,
action
of the
thyroid
AND
with
ats active
tratssfer
to use this cell
hormomse
957
ANGERER
cotsjutsctioms
in
and possibly
imsvolvitsg
it may be advamstageous
26
of the
acid,2124
WATKINS,
oms a cellular
etszymes
for
a glycolytic
of glucose
across
the cell
for determinimig
the msature
I)asis.
SUMMARY
Blood
was
obtaimsed
from
euthyroid,
(B)
hypothyroid.
suspemided
The erythrocytes
its Krebs-phosphate
technic.
When
compared
cent
untreated
the
with
chaisge
is
for
plotted
curve
of
to
exponenstial
this
for
appears
to
studio
centrationes
non-nucleate
inter
iste
que
esseva
interprendite
de hormon
e (2) pro
thyroide
establir
metabolismo
se exprime
Nos
(a)
esseva
abluite
iste
specimens
groups
B, C atsd
re-
D are
-
+23
per (‘etst and
15 per
values
whets Qo. values
for
group
(excludimsg
group
C)
are
a
differeva
inter
de
serie
(B),
02
-
median
iodo
de
del
iste
le effecto
ab
(‘irculante
de iodo
quatro
de
phosphatic
respirometric
de
del
Ic serie
pro
cento
como
esseva
inscril)ite
curva
iodo
in
ha naturalmente
classificabile
datos
de O
per
Le
(B),
ben
nulle
(C)
iste
del
in relation
isto
in
del
un
il appare
exponential.
Le
que
e
iste
valores
in cata
Ab
in Ic serie
(B),
(C),
inter
de coordinatas
del sero.
de ordine
in
le erythro-
Ic prime
individual
pro
obtenite
Ic series
Solo
systema
un
intraprotemic
per un curva
sero.
in preparation
Le
significative.
de O per Ic erythrocytos
inter
le series
(A) e
intraproteinic
ientes
controlo-e
patientes
thyroide
del
Irebs
ivemente.
con-
(C) hyperthyroide
(‘ata serie de patientes
Warhimrg.
statisticamente
del
hormon
pat
consumption
de
respect
vane
de erythrocytos
quantitative
int-raproteinic
series
in un solution
median
de
respiratori
un correlation
del
valores
le erythrocytos
per
e (D)
consumption
determinar
concentrationes
(A)-i.e.
15
al correspondente
valores
de
Ic datos
es le melio
representate
median
del
le ordinata
pro
le metabolismo
que possibile
in tanto
le valores
que
+23,
e
considerate
consumption
(A),
(1)
de sanguine
le te(’hnica
indicava
INTERLINGUA
(B) hyperthyroide
sin tractamento,
hypothyroide.
Le erythrocytos
ab
e re-suspendite
secundo
(D) per +92,
valores
esseva
series
i)e
super
e le vane
euthyroide,
a iodo, e (D)
studios
cytos
washed
atsd
respirometric
P131 cotscemstration
(.sg. per cent),
the (ur\’e
of best prOl)able
fit for tsese
IN
in le correspondente
obteneva
como
tractate
studios
: (A)
amid (I))
group.
SUMMARIO
Iste
patietit
serum
were
Warburg
patietit
per cent,
meats Q.
groups
hyperthyroid
Q2 value for the iodimme-treated
hyperthyroid
group
falls
mi the
curve
(fig. 1 ) hetweets
the eimthyroid
arid utstreated
hyperti
yroid
the meats
serum
PBI
value
(abscissa),
of course,
has mio meats-
data.
The mean
ordimsate
of this
groups,
though
ing
order
from
its each
imsto four
patiemst
group
study
by the
a +92
in their
respective
divided
iodimse-treated
obtained
patieist
their
‘ere
(C)
A (comstrol),
respectively,
each
relative
‘ho
from
each
solutioms
for
erythrocytes
group
noted,
erythrocytes
patiemsts
hyperthyroid,
se trova
caso
que
valor
super
Ic valor
signification.
REFERENCES
1
RAMSEY,
mature
R.
AND
rabbit
WARREN,
erythroc’tes.
C. 0.:
The
Quart.
rate
of respiration
J. Exper.
Physiol.
in
22:
erthirocvles.
49. 1932.
2. The
rate
in
From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
958
OXYGEN
J.
2 GONZALEZ-Q.,
3
tomizedi
rats
502, 1947.
ANGELONE,
L.
nucleated
AND
with
J.
MORTON,
-,
6
metabolism
Unupublished
6
SCOTT,
7
ISSEKUTZ,
L.,
ANt)
B.,
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From www.bloodjournal.org by guest on July 31, 2017. For personal use only.
1954 9: 953-958
Oxygen Consumption of Erythrocytes from Patients with Various Thyroid
Conditions Related to Their Respective Serum Protein−−Bound Iodine
Concentrations
LUIS ANGELONE, DAVID H. WATKINS and CLIFFORD A. ANGERER
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