100
95
90
85
80
75
70
65
Transmission (%)
60
55
50
45
40
35
30
25
20
15
10
5
0
420
430
440
450
460
470
480
490
500
510
520
Wavelength (nm)
530
540
550
560
570
580
590
600
AT Bandpass Sets
AT Longpass Sets
Filter Set
Fluorochrome
Filter Set
DAPI/Hoechst/AlexaFluor 350 and similar
39000
DAPI and similar
19000
CFP/Cerulean and similar
39001
Coumarin/Pacific Blue and similar
19011
GFP/FITC/Cy2/AlexaFluor 488 and similar
39002
Aqua/CFP and similar
19001
YFP/Venus/Citrine and similar
39003
GFP/FITC and similar
19002
TRITC/Cy3/TagRFP/AlexaFluor 546 and similar
39004
TRITC and similar
19004
Red-shifted TRITC and similar
39005
Texas Red/mCherry and similar
19006
Texas Red/mCherry/AlexaFluor594 and similar
39010
Auramine and similar
19008
Cy5/AlexaFluor/Draq5 and similar
39007
FM 1-43/Chlorophyll and similar
19010
Lucifer Yellow/Tetracycline/AlexaFluor 430 and similar
39008
FluoroGold/Calcofluor White and similar
19012
Acridine Orange/Di-8-ANEPPS and similar
39009
Fluorochrome
100
95
90
85
80
75
70
65
Transmission (%)
60
55
50
45
40
35
30
25
20
15
10
5
0
500
510
520
530
540
550
560
570
580
590
600
610
620
630
640
Wavelength (nm)
650
660
670
680
690
700
710
720
730
740
750