Figure S1
PANC-1
CD44-
PANC-28
CD44+
CD44-
CD44+
Aii
CD44
-
250
CD44+
KLF4
KLF4
Fold
B
PANC-1
Diameter of
spheres (μm)
Ai
1.00
0.10
1.00
0.30
1.00
0.10
β-Actin
RF4-L3.7SF
200
200
150
150
0
0
Di
Dox (ng/ml)
10
OD Values
100 μm
Eii
H&E staining
Dox
Ctr
Liver metastatic nodules
100
Dii
24 Hrs
Ei
Dox 50
L3.7
100 μm
Dox 75 ng/ml
Dox 100 ng/ml
100 μm
100 μm
Dox 0
Dox 50
0
RF4-SW620SF
Dox 0
Dox 0
RF4-L3.7SF
**
50
50
Tubulin
RF4-SW620SF
100
*
100
Dox 0
C
250
Ctr
48 Hrs
Dox
72 Hrs
Figure S2
A
B
723G
723K
924G
924K
723
G
K
G
924
K
WT
F/F
-425bp
-296bp
-172bp
Figure S3
CD44
Villin-Cre;Klf4+/+
Klf4
100μm
100μm
100μm
Villin-Cre;Klf4f/f
100μm
Figure S4
(Revised)
PANC-1-Ctr
PANC-1-SF
B
2
Relative expression
A
1.5
KLF4
**
CD44
1
**
0.5
0
Figure S5
A
5´
constant
constant
S1-5
S6-10
3´ CD44s
Variant (V1~V10)
5´
constant
V6 V7 V8 V9 V10 constant
V1 V2 V3 V4 V5
S1-5
Bi
Bii
SW480
Mock Ctr-si KLF4-si
M
M
3´ CD44v
S6-10
Di
C
COLO357 L3.7 SW480 SW620
CD44v
723G 723K
CD44v
CD44s
CD44s
ESRP1
ESRP1
β-actin
β-Actin
Dii
Diii
BxPC3
NT
5316
Dox
(ng/ml
RF4-L3.7
0
50
RF4-SW620
0
50
ESRP1
GAPDH
Eii
Ei
293
Eiii
PANC-1
pCtr
pKLF4
KLF4
F-KLF4
150 kDa
CD44v
100 kDa
CD44s
CD44v
CD44s
CD44v
CD44s
72 kDa
α-tubulin
GAPDH
α-tubulin
Mock
Ctr-si
F4-si
pCtr
pKLF4
Figure S6
0h
B
Vimentin
Fold
48h
Colo357
A
1.0
>10
1.0
0.3
0h100±4%
48h 72±4%
Fold
L3.7
E-Cadherin
β-Catenin
Fold
1.0
2.3
α-Tubulin
L3.7
Ciii
Civ
6
*
4
2
0
COLO357
L3.7
30
**
20
10
0
COLO357
L3.7
H&E staining
Liver weight (g)
No of metastases
Cii
COLO357
Ci
100±3%
COLO357
17±5%**
L3.7
Figure S7
B
Primary pancreatic tumor
Liver metastatic tumor
KLF4
A
Pancreas
200 μm
200 μm
200 μm
200 μm
CD44
Metastasize
Pancreatic xenograft tumor
KLF4 staining
Ci
200 μm
50 μm
D
Cii
Liver metastasis
200 μm
Adjacent Normal Tissue
50 μm
Primary Pancreatic Tumor
15
10
5
**
0
PT
MT
CD44
KLF4 Score
20
200 μm
200 μm