Weighted models for insulin
Detected by scanone
# transcripts that match
weighted insulin model
in each of 4 tissues:
Detected by Ping’s multiQTL model
tissue
# transcripts
Islet
1984
Adipose
605
Liver
485
Gastroc
404
Histogram of transcript-to-insulin correlations
Islet – enrichment for 1984 transcripts
Set
GOCC
GOCC
GOCC
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOMF
GOMF
GOBP
GOBP
GOBP
GOBP
GOCC
KEGG
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
Pvalue
Qvalue
3.70E-06
0.0073
3.45E-05
0.0294
4.48E-05
0.0294
8.16E-05
0.0402
0.0002
0.0761
0.0003
0.0761
0.0004
0.0761
0.0004
0.0761
0.0004
0.0761
0.0003
0.0761
0.0003
0.0761
0.0006
0.0895
0.0006
0.0895
0.0012
0.1469
0.0012
0.1469
0.0011
0.1469
0.0013
0.1469
0.0025
0.2361
0.0029
0.2361
0.0029
0.2361
0.0033
0.2361
0.0034
0.2361
0.0035
0.2361
0.0036
0.2361
0.0043
0.2361
0.0047
0.2361
0.0047
0.2361
0.0047
0.2361
0.0047
0.2361
Count
192
177
220
37
66
11
35
35
35
381
3
44
44
3
3
536
6
93
3
46
4
22
5
16
7
2
2
2
6
Size
2037
1919
2483
279
623
48
281
281
281
4802
3
386
386
4
4
7011
17
1021
5
442
10
172
16
111
31
2
2
2
24
Term
extracellular region part
extracellular space
extracellular region
enzyme linked receptor protein signaling pathway
nervous system development
neuromuscular process
cell projection organization and biogenesis
cell part morphogenesis
cell projection morphogenesis
protein binding
siRNA binding
cell morphogenesis
cellular structure morphogenesis
axon choice point recognition
rhythmic synaptic transmission
membrane
Polyunsaturated fatty acid biosynthesis
anatomical structure morphogenesis
retinol metabolic process
cytoskeleton organization and biogenesis
microtubule bundle formation
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of catabolic process
forebrain development
neuromuscular process controlling balance
rhythmic excitation
protein amino acid de-ADP-ribosylation
ventricular cardiac muscle cell development
cytokinesis
Islet – modules
17
2
16
14
19
12
9
chromosomes
Insulin trait
Islet – enrichment for modules
Module
BLUE
GREEN
PURPLE
BLACK
MAGENTA
YELLOW
RED
BROWN
TURQUOISE
PINK
Pvalue
Qvalue Count
Size
0.0005
0.0006
0.0009
0.0012
0.0008
0.0055
0.0056
0.0463
0.0470
0.0507
0.0593
0.0457
0.0970
0.0970
30
18
11
19
4
2
10
1068
511
241
590
76
20
707
0.0011
0.0078
2.54E-05
0.0001
0.0004
0.0005
0.0006
0.0009
0.0011
0.0012
0.0026
0.0026
0.0026
0.0017
0.0026
0.0057
0.0002
0.0003
0.0003
0.0004
0.0004
0.0092
0.0165
0.0138
0.0011
0.0040
0.0040
0.0040
0.0040
0.0041
0.0041
0.0041
0.0675
0.0675
0.0675
0.0619
0.0619
0.1442
0.0830
0.0830
0.0830
0.0830
0.0608
0.0612
7
2
7
5
5
5
5
5
4
4
7
7
7
2
5
4
17
10
7
40
2
4
2769
68
313
179
225
228
239
266
162
163
281
281
281
13
200
96
279
115
57
1021
14
384
Term
biosynthetic process
cellular lipid metabolic process
lipid biosynthetic process
lipid metabolic process
phosphate transport
intermediate filament-based process
ion transport
nucleobase, nucleoside, nucleotide and nucleic acid
metabolic process
sensory perception of sound
cell cycle process
microtubule-based process
mitotic cell cycle
M phase
cell division
cell cycle phase
mitosis
M phase of mitotic cell cycle
cell projection organization and biogenesis
cell part morphogenesis
cell projection morphogenesis
steroid hormone receptor signaling pathway
reproductive process
response to pheromone
enzyme linked receptor protein signaling pathway
morphogenesis of an epithelium
morphogenesis of embryonic epithelium
anatomical structure morphogenesis
vesicle organization and biogenesis
regulation of apoptosis
Insulin
chromosomes
Adipose – enrichment for 605 transcripts
Set
GOCC
GOMF
GOBP
GOBP
KEGG
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
Pvalue
Qvalue
4.87E-07
0.0005
9.54E-05
0.0531
0.0004
0.0846
0.0004
0.0846
0.0003
0.0846
0.0006
0.0886
0.0007
0.0886
0.0010
0.1077
0.0020
0.1790
0.0030
0.1790
0.0038
0.1790
0.0040
0.1790
0.0042
0.1790
0.0043
0.1790
0.0046
0.1790
0.0062
0.1790
0.0086
0.1790
0.0087
0.1790
0.0087
0.1790
0.0090
0.1790
0.0098
0.1790
0.0121
0.1790
0.0130
0.1790
0.0134
0.1790
0.0136
0.1790
0.0158
0.1790
0.0173
0.1790
0.0197
0.1790
0.0206
0.1790
Count
174
151
11
11
4
4
15
40
3
7
4
5
3
4
24
3
16
3
3
24
13
6
4
17
6
16
3
7
7
Size
5332
4801
132
135
12
17
237
993
11
79
27
44
14
28
558
16
340
18
18
590
257
79
38
388
81
364
23
113
114
Term
cytoplasm
catalytic activity
hexose metabolic process
monosaccharide metabolic process
Circadian rhythm
T cell selection
cellular carbohydrate metabolic process
intracellular signaling cascade
inositol metabolic process
positive regulation of signal transduction
monosaccharide biosynthetic process
cell redox homeostasis
thymic T cell selection
alcohol biosynthetic process
catabolic process
polyamine metabolic process
carbohydrate metabolic process
glucosamine metabolic process
N-acetylglucosamine metabolic process
lipid metabolic process
alcohol metabolic process
one-carbon compound metabolic process
protein ubiquitination
regulation of signal transduction
carbohydrate biosynthetic process
small GTPase mediated signal transduction
amino sugar metabolic process
peptidyl-amino acid modification
lipid catabolic process
Adipose – modules
19
2
9
14
17
12
chromosomes
Insulin trait
Adipose – enrichment for modules
Module
GREEN
PINK
BLACK
YELLOW
TURQUOISE
BLUE
PURPLE
BROWN
RED
MAGENTA
Pvalue Qvalue Count
0.014 0.0560 4
0.019 0.0654 1
0.001 0.0430 4
0.004 0.0516 5
0.015 0.0743 1
0.003 0.0366 2
0.007 0.0436 2
0.021 0.0684 1
0.002 0.0173 2
0.002 0.0173 3
0.007 0.0280 2
0.010 0.0287 2
0.014 0.0327 2
0.019 0.0342 2
0.001 0.0713 4
0.002 0.0713 2
0.002 0.0713 3
0.003 0.0713 10
1.60E-06 0.0004 4
4.90E-05 0.0023 3
0.0002 0.0039 7
0.0002 0.0054 7
0.0003 0.0061 3
0.0019 0.0652 3
0.0080 0.0869 3
0.0002 0.0234 3
0.0005 0.0234 2
0.0006 0.0234 3
0.0009 0.0234 2
0.0011 0.0260 2
0.0017 0.0346 2
0.0044 0.0446 2
0.0049 0.0446 2
0.0051 0.0446 2
0.0062 0.0446 2
0.0069 0.0475 3
0.0153 0.0969 1
0.0278 0.1410 1
0.0030 0.0789 2
0.0147 0.1436 1
Size
605
12
364
993
13
46
79
13
28
113
56
69
82
97
70
12
44
590
17
14
221
237
25
237
392
58
16
78
21
24
29
47
50
51
56
187
12
22
79
14
Term
proteolysis
nucleoside diphosphate metabolic process
small GTPase mediated signal transduction
intracellular signaling cascade
cellular protein complex disassembly
methylation
one-carbon compound metabolic process
sulfate transport
response to hormone stimulus
peptidyl-amino acid modification
hemostasis
regulation of body fluid levels
lipid transport
glycoprotein metabolic process
rhythmic process
negative regulation of blood pressure
cell redox homeostasis
lipid metabolic process
T cell selection
thymic T cell selection
leukocyte activation
cell activation
hexose biosynthetic process
synapse
cell junction
amine biosynthetic process
polyamine metabolic process
nitrogen compound biosynthetic process
biogenic amine biosynthetic process
amino acid derivative biosynthetic process
sulfur compound biosynthetic process
detection of abiotic stimulus
inner ear morphogenesis
detection of external stimulus
ear morphogenesis
positive regulation of cell proliferation
hydrogen peroxide metabolic process
cellular response to stimulus
one-carbon compound metabolic process
protein targeting to membrane
Insulin
chromosomes
Liver – enrichment for 485 transcripts
Set
GOBP
GOBP
GOMF
GOMF
GOBP
GOBP
GOBP
GOCC
GOCC
GOMF
GOCC
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
Pvalue
Qvalue
0.0005
0.0584
0.0006
0.0584
0.0005
0.0584
0.0009
0.0584
0.0012
0.0658
0.0016
0.0808
0.0020
0.0882
0.0023
0.0922
0.0024
0.0922
0.0026
0.0945
0.0028
0.0990
0.0036
0.1197
0.0044
0.1197
0.0048
0.1197
0.0055
0.1197
0.0063
0.1197
0.0070
0.1197
0.0076
0.1197
0.0106
0.1197
0.0109
0.1197
0.0109
0.1197
0.0109
0.1197
0.0122
0.1197
0.0122
0.1197
0.0138
0.1197
0.0144
0.1197
0.0144
0.1197
0.0149
0.1197
0.0151
0.1197
Count
4
4
114
3
3
11
3
180
9
5
9
3
4
6
5
7
4
3
6
3
3
3
3
3
5
7
6
3
6
Size
20
21
4801
11
12
205
14
8478
155
52
159
17
36
84
61
117
41
22
99
25
25
25
26
26
76
137
106
28
107
Term
nucleotide catabolic process
glutathione metabolic process
catalytic activity
nucleotidase activity
response to dsRNA
regulation of cell differentiation
thymic T cell selection
intracellular
microsome
transferase activity, transferring alkyl or aryl (other than methyl) groups
vesicular fraction
T cell selection
regulation of lymphocyte differentiation
microtubule-based movement
sulfur metabolic process
bone remodeling
dendrite development
positive regulation of T cell differentiation
cytoskeleton-dependent intracellular transport
bone mineralization
fat cell differentiation
positive regulation of lymphocyte differentiation
regulation of ossification
T cell differentiation in the thymus
phosphate transport
tissue remodeling
ossification
gene silencing
biomineral formation
Liver – modules
14
9
12
17
chromosomes
Insulin trait
Liver – enrichment for modules
Module
YELLOW
GREEN
RED
BROWN
TURQUOISE
BLUE
Pvalue
Qvalue Count
0.0004
0.0592
2
0.0035
0.0634
7
0.0050
0.0634
2
0.0132
0.0144
0.0144
0.0090
0.0868
0.0882
0.0882
0.0128
1
1
1
1
0.0135
0.0162
0.0005
0.0007
0.0012
0.0015
0.0017
0.0021
0.0032
0.0067
0.0077
0.0020
0.0020
0.0022
0.0025
0.0025
0.0029
0.0036
0.0062
0.0054
0.0076
0.0082
0.0102
0.0109
0.0115
0.0123
0.0158
0.0177
0.0186
0.0170
0.0189
0.0210
0.0210
0.0210
0.0222
0.0310
0.0313
0.0313
0.0648
0.0648
0.0648
0.0648
0.0648
0.0648
0.0648
0.0648
0.0730
0.0764
0.0764
0.0764
0.0764
0.0764
0.0764
0.0764
1
2
2
2
2
2
2
2
2
2
4
4
3
3
2
2
3
4
8
2
2
2
5
4
2
2
3
Size
Term
15 lamellipodium biogenesis
1021 anatomical structure morphogenesis
52 cell projection biogenesis
negative regulation of protein amino acid
11 phosphorylation
12 negative regulation of phosphorylation
12 negative regulation of hydrolase activity
12 nucleoside diphosphate metabolic process
antigen processing and presentation of peptide antigen
18 via MHC class I
266 cell cycle phase
14 thymic T cell selection
17 T cell selection
22 positive regulation of T cell differentiation
25 positive regulation of lymphocyte differentiation
26 T cell differentiation in the thymus
29 regulation of T cell differentiation
36 regulation of lymphocyte differentiation
53 positive regulation of T cell activation
340 regulation of cell proliferation
84 microtubule-based movement
40 protein polymerization
41 dendrite development
12 neuron recognition
12 response to dsRNA
45 protein modification by small protein conjugation
99 cytoskeleton-dependent intracellular transport
436 response to chemical stimulus
21 glutathione metabolic process
25 fat cell differentiation
26 regulation of ossification
250 gamete generation
164 chromatin modification
31 regulation of bone remodeling
32 regulation of tissue remodeling
100 response to bacterium
Insulin
chromosomes
Gastroc – enrichment for 404 transcripts
Set
GOBP
GOBP
GOMF
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
GOBP
Pvalue
Qvalue
0.0003
0.1718
0.0012
0.1718
0.0012
0.1718
0.0019
0.1748
0.0047
0.1748
0.0065
0.1748
0.0075
0.1748
0.0080
0.1748
0.0089
0.1748
0.0093
0.1748
0.0094
0.1748
0.0123
0.1748
0.0123
0.1748
0.0125
0.1748
0.0125
0.1748
0.0134
0.1748
0.0148
0.1748
0.0155
0.1748
0.0157
0.1748
0.0163
0.1748
0.0172
0.1748
0.0174
0.1748
0.0178
0.1748
0.0181
0.1748
0.0191
0.1748
0.0198
0.1748
0.0198
0.1748
0.0198
0.1748
0.0199
0.1748
Count
4
3
5
28
68
112
112
71
4
77
5
2
2
3
3
15
3
4
119
4
2
3
21
7
76
2
2
2
12
Size
21
14
49
993
3246
5957
5980
3492
52
3869
82
11
11
31
31
503
33
61
6550
62
13
35
815
170
3923
14
14
14
388
Term
response to toxin
regulation of I-kappaB kinase/NF-kappaB cascade
lipid transporter activity
intracellular signaling cascade
regulation of cellular process
primary metabolic process
cellular metabolic process
regulation of biological process
antigen processing and presentation
biological regulation
lipid transport
response to metal ion
positive regulation of I-kappaB kinase/NF-kappaB cascade
neural crest cell development
neural crest cell differentiation
cell proliferation
antigen processing and presentation of peptide antigen
regulation of protein amino acid phosphorylation
metabolic process
regulation of MAP kinase activity
embryonic forelimb morphogenesis
I-kappaB kinase/NF-kappaB cascade
positive regulation of cellular process
heart development
biopolymer metabolic process
tricarboxylic acid cycle intermediate metabolic process
response to inorganic substance
dicarboxylic acid metabolic process
regulation of signal transduction
Gastroc – modules
14
2
12
16
19
17
9
Insulin trait
chromosomes
Gastroc – enrichment for modules
Module
GREEN
YELLOW
BLUE
RED
BLACK
TURQUOISE
BROWN
PINK
Pvalue Qvalue Count Size
Term
0.0157 0.1870 1
15 vacuolar transport
0.0157 0.1870 1
15 lamellipodium biogenesis
0.0167 0.1870 1
16 activated T cell proliferation
0.0175 0.0472 1
13 regulation of protein polymerization
0.0177 0.0472 13 5957 primary metabolic process
0.0183 0.0472 13 5980 cellular metabolic process
regulation of small GTPase mediated signal
0.0188 0.0472 2
157 transduction
0.0088 0.0231 13 3869 biological regulation
0.0102 0.0231 2
85 positive regulation of protein kinase activity
0.0114 0.0231 2
90 positive regulation of kinase activity
0.0116 0.0231 2
91 positive regulation of transferase activity
0.0161 0.0231 2
108 negative regulation of signal transduction
0.0180 0.0231 11 3246 regulation of cellular process
0.0188 0.1749 1
12 response to osmotic stress
0.0266 0.1930 1
17 gluconeogenesis
antigen processing and presentation of peptide
0.0281 0.1930 1
18 antigen via MHC class I
positive regulation of I-kappaB kinase/NF0.0058 0.0137 1
11 kappaB cascade
0.0074 0.0137 1
14 heart looping
regulation of I-kappaB kinase/NF-kappaB
0.0074 0.0137 1
14 cascade
0.0013 0.0692 17 1294 post-translational protein modification
0.0018 0.0692 36 3923 biopolymer metabolic process
0.0024 0.0692 18 1488 protein modification process
0.0032 0.0692 18 1525 biopolymer modification
0.0003 0.0105 2
11 response to metal ion
0.0006 0.0151 2
14 response to inorganic substance
antigen processing and presentation of peptide
0.0007 0.0159 2
16 antigen via MHC class II
antigen processing and presentation of
0.0007 0.0159 2
16 exogenous peptide antigen via MHC class II
antigen processing and presentation of peptide
0.0008 0.0163 2
17 or polysaccharide antigen via MHC class II
antigen processing and presentation of
0.0009 0.0168 2
18 exogenous peptide antigen
0.0013 0.0212 2
21 response to toxin
antigen processing and presentation of
0.0016 0.0226 2
23 exogenous antigen
0.0016 0.0153 2
82 hormone metabolic process
0.0083 0.0163 1
11 sequestering of lipid
0.0090 0.0173 1
12 negative regulation of hydrolase activity
0.0097 0.0176 1
13 proximal/distal pattern formation
0.0097 0.0176 1
13 embryonic forelimb morphogenesis
Insulin
chromosomes
4 tissues combined – modules
Insulin trait
Insulin
adipose
gastroc
islet
liver
total
turquoise
63
83
546
17
709
blue
20
8
308
12
348
brown
33
96
46
128
303
yellow
68
35
165
33
301
green
43
56
132
41
272
red
33
31
198
9
271
black
164
13
58
23
258
pink
32
28
88
98
246
magenta
53
9
149
11
222
purple
34
12
143
19
208
greenyellow
40
31
43
45
159
tan
3
1
106
1
111
salmon
19
1
1
48
69
total
605
404
1983
485
3477