Calculating water potential
Ψ = Ψp + Ψs
The combined effects of these two factors:
1. Solute concentration
2. Pressure
are incorporated into a single measurement
called Water Potential or
Ψ
Water will move across a membrance
from the solution with higher water
potential to the solution with the
lower water potential.
Water Potential
– Water potential is determined by solute potential
and pressure potential.
Ψ = Ψp + Ψs
– Water moves from regions of high water potential to
regions of low water potential.
Ψs
Solute (osmotic) potential
Once you know the solute concentration,
you can calculate solute potential using
the following formula:
• Solute potential (ΨS ) = –iCRT
Ψs
Solute (osmotic) potential
• Pure water has a solute potential (Ψs) of zero.
Solute potential can never be positive.
• Adding more solute is a negative experience;
the solute potential becomes negative.
Ψs = − iCRT
Solute potential
• i = The ionization constant
– for NaCl this would be 2;
– for sucrose or glucose, this number is 1
• C = Molar concentration of solute
– Can be determined mathematically, graphically by
determining the iso-osmotic concentration, or you will be
told this value.
• R = Pressure constant = 0.0831 liter bar/mole K
• T = Temperature in degrees Kelvin = 273 + °C of
solution
Pressure Potential – the sum of all
pressure on water.
In units of pressure: MPa or Bars
• Turgor pressure – forced caused by cell
membrane pushing against cell wall.
• Wall pressure – an equal and opposite
force exerted by cell wall. Counteracts the
movement of water due to osmosis.
• Other pressures – tension, cohesion,
atmospheric, root, etc.
Calculating Water Potential
• Water potential is calculated using the following
formula:
• Ψ = ΨP + ΨS
• Pressure potential (ΨP ): In a plant cell, pressure
exerted by the rigid cell wall that limits further
water uptake.
• Solute potential (ΨS ): The effect of solute
concentration.
– Pure water at atmospheric pressure has a
solute potential of zero.
Water potential