Kinetex 1.7 µm UHPLC Columns

Core-Shell Technology
Kinetex
1.7 µm UHPLC
Columns
The chosen core-shell brand to drive
UHPLC performance to the next level
Core-Shell Technology
Core-Shell Technology
Simply Advantageous
The band broadening (wide peaks) and lengthy retention
times of traditional fully porous products can limit your
results. Now you can use the incredible efficiency levels of
Kinetex Core-Shell Technology to achieve shorter run times,
higher levels of sensitivity, and overall better UHPLC results.
1
Kinetex Core-Shell
1
Traditional Fully Porous
20
Get faster,
better results
with me!
15
10
APP ID 22805
mAU
4
2
2
5
4
3
3
0
28
30
32
34
Kinetex Core-Shell
Traditional Fully Porous
Phenomenex
l
WEB: www.phenomenex.com
36
min
The First 1.7 µm
Core-Shell Column
Core-Shell Technology
Upgrade the efficiency and performance of your current traditional sub2 μm column with a Kinetex 1.7 μm core-shell column. Extra resolution,
higher peak capacities, and greater sensitivity are all within your reach with
Kinetex Core-Shell Technology.
Kinetex 1.7 µm XB-C18
%
66
19804
AU
0.035
0.030
0.025
Higher Resolution
(between peaks 1 and 2)
1
138%
4
Higher Resolution
(between peaks 5 and 6)
21%
Higher Resolution
(between peaks 7 and 8)
6
2
0.020
8
5
7
3
0.010
APP ID 19804
0.015
0.005
0
2
3
4
min
Waters® ACQUITY® BEH 1.7 µm C18
AU
0.035
0.030
0.025
1
0.020
4
6
2
8
5
0.010
3
7
APP ID 19805
0.015
0.005
0
2
3
Conditions for both columns:
Column: Kinetex 1.7 µm XB-C18
ACQUITY BEH 1.7 µm C18
Dimensions: 150 x 2.1 mm
Mobile Phase: A: Milli-Q® Water
B: Acetonitrile
Gradient: Time (min)
%B
0
30
5
100
Flow Rate: 0.5 mL/min
Temperature: Ambient
Detection: UV @ 210 nm (ambient)
Backpressure: 891 bar (Kinetex)
935 bar (ACQUITY)
Instrument: Waters ACQUITY UPLC®
Sample: 1. Hydrocortisone
2. Cortisone
3. Corticosterone
4. Cortisone-21-Acetate
5. DHEA (Dehydroepiandrosterone)
6. 17-Hydroxyprogesterone
7. Deoxycorticosterone
8. Progesterone
Comparative separations may not be representative of all applications.
Phenomenex
4
l
WEB: www.phenomenex.com
min
Superior and Faster
Resolving Power
Core-Shell Technology
The increased efficiency of Kinetex 1.7 μm allows you to use shorter
columns for faster run times while keeping optimal performance. Now
it’s time for you to challenge your difficult separations with the superior
efficiency and resolving power of a Kinetex 1.7 μm core-shell column.
Faster analysis, better
sensitivity, and overall
better resolution!
Kinetex 1.7 µm C18
APP ID 20686
Intensity, cps
1.4e4
7000
0
0.2
0.4
0.6
0.8
1.0
1.2
1.4
1.6
1.8
min
1.0
1.2
1.4
1.6
1.8
min
1.0
1.2
1.4
1.6
1.8
min
5500
APP ID 20686
Intensity, cps
Fully Porous 3.5 µm C18
0
0.2
0.4
0.6
0.8
Fused-Core® 2.7 µm C18
6000
APP ID 20686
Intensity, cps
1.15e4
0
0.2
0.4
0.6
0.8
Conditions for all columns:
Columns: Fully porous 3.5 µm C18 (50 x 2.1 mm)
Fused-Core 2.7 µm C18 (50 x 2.1 mm)
Kinetex 1.7 µm C18 (30 x 2.1 mm)
Mobile Phase: A: 10 mM Ammonium Formate
B: Acetonitrile
Gradient: Time (min)
%B
0
5
2
100
2.1
5
Flow Rate:
Temperature:
Detection:
Sample:
0.7 mL/min
22 °C
MS/MS (SCIEX API 4000™)
Lorazepam glucuronide
Comparative separations may not be representative of all applications.
Phenomenex
l
WEB: www.phenomenex.com
Core-Shell Technology
Trust / Consistency
Highly sensitive UHPLC separations are dependent upon consistent
quantitation and consistent results. With the reliability of the Kinetex columns
and media batches, you no longer have to settle for the inconsistent results
of inferior products that exist on the market. Our highest standards of
quality will ensure that you are fully satisfied with each and every Kinetex
column!
mAU
60
Batch 12
5 6
50
APP ID 22782
7
40
2
30
20
1
4
3
10
0
0
1
2
3
min
4
mAU
60
Batch 13
5 6
50
7
APP ID 22782
40
2
30
20
1
4
3
10
0
0
1
2
3
min
4
mAU
60
Batch 14
5 6
50
APP ID 22782
7
40
2
30
20
1
4
3
10
0
0
1
2
3
4
Detection: UV @ 254 nm (ambient)
Sample: 1. Uracil
2. Pindolol
3. Chlorpheniramine
4. Nortriptyline
5. 3-Methyl-4-Nitrobenzoic acid
6. 5-Methyl Salicyl Aldehyde
7. Hexaphenone
Conditions same for all batches:
Column: Kinetex 1.7 µm F5
Dimensions: 50 x 4.6 mm
Mobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Acetonitrile
Gradient: 5-95 % B over 5 min
Flow Rate: 1.85 mL/min
Temperature: 30 ˚C
Comparative separations may not be representative of all applications.
Phenomenex
l
WEB: www.phenomenex.com
min
Upgrade from
Hybrid Materials
Core-Shell Technology
By upgrading from fully porous hybrid UHPLC columns to the Kinetex
1.7 μm EVO C18, you can keep the pH stability but gain much greater
performance. With the core-shell advantage at your disposal your new
methods will be faster and more sensitive, leaving room for greater
productivity.
Berberine Standard
mAU
120
Kinetex 1.7 µm
EVO C18
57%
Increase in Peak
Height Response
100
Waters® ACQUITY®
BEH 1.7 µm C18
80
60
APP ID 23111 and 23109
40
Greater Resolution
of Impurities
20
0
1
2
3
Conditions for both columns:
Column: Kinetex 1.7 µm EVO C18
ACQUITY BEH 1.7 µm C18
Dimensions: 50 x 2.1 mm
Mobile Phase: A: 0.1 % Formic Acid in Water
B: 0.1 % Formic Acid in Methanol
Gradient: Time (min)
%B
0
2
5
95
Flow Rate: 0.5 mL/min
Temperature: 30 º C
Detection: UV @ 254 nm (ambient)
Sample: 1. Berberine
Comparative separations may not be representative of all applications.
Phenomenex
l
WEB: www.phenomenex.com
min
Core-Shell Technology
UHPLC Compatible
Kinetex columns are compatible with ALL UHPLC Instruments.
Kinetex on
Agilent® 1290
Kinetex on
JASCO® X-LC
Kinetex on
Shimadzu® Nexera®
Kinetex on
Waters® ACQUITY UPLC®
Need help installing your Kinetex column?
www.phenomenex.com/kinetex/technicalresources
Phenomenex
l
WEB: www.phenomenex.com
Utilizing Powerful
Selectivities
Core-Shell Technology
With the combination of the novel Kinetex EVO C18, F5, and Biphenyl
selectivities at your disposal, reversed phase UHPLC method development
can be taken to the next level. With its exceptional peak shape for basic
compounds, the EVO C18 is a dynamic tool that gives core-shell high
performance while allowing retention to be enhanced by adjusting the pH
of the mobile phase.
Kinetex 1.7 µm EVO C18
mAU
800
TMS
APP ID 23079
pH 2.5
600
TMS
400
EVO
200
1
C18
0
1
2
3
4
5
6
7
min
Utilize High pH Stability of EVO
C18 to Enhance Retention
Kinetex 1.7 µm EVO C18
mAU
700
600
TMS
pH 10
TMS
APP ID 23082
500
400
300
1
EVO
200
C18
100
0
0
1
2
3
4
5
6
Conditions for all columns same except where noted:
Column: Kinetex 1.7 µm EVO C18
Dimensions: 50 x 2.1 mm
Part No.: 00B-4726-AN
Mobile Phase: A: 20 mM Potassium phosphate (pH 2.5)
B: Methanol
Mobile Phase: A: 20 mM Potassium phosphate (pH 10)
B: Methanol
Gradient: Time (min) % B
0
5
8
90
Flow Rate: 0.5 mL/min
Temperature: 30 º C
Detection: UV @ 210 nm (ambient)
Sample: 1. Fluvoxamine
Phenomenex
l
WEB: www.phenomenex.com
7
min
Mo
Orth
Sol
Core-Shell Technology
Next to the EVO C18, the orthogonal Kinetex F5 and Biphenyl will drive
enhancement of reversed phase methods with their versatile array of
interaction mechanisms. Varying degrees of electrostatic, aromatic, reversed
phase, steric, and hydrogen bonding between the F5 and Biphenyl mean
that these two stationary phases are both excellent alternatives to a C18.
Additionally the F5 is known for its ability to separate isomeric species,
while the pi electron cloud created by the dual ring of the Biphenyl phase
acts like a weak negative charge and aids basic compound retention.
Kinetex 1.7 µm F5
mAU
700
600
500
400
300
200
100
0
F
F
F
pH 2.5
F
TMS
TMS
F5
0
APP ID 23080
F
1
1
2
3
4
5
6
7
min
Kinetex 1.7 µm Biphenyl
mAU
700
600
500
TMS
pH 2.5
TMS
APP ID 23081
ove to
hogonal
lutions!
Utilizing Powerful
Selectivities
400
300
Biphenyl
200
1
100
0
0
1
2
3
4
5
Conditions for all columns:
Column: Kinetex 1.7 µm F5
Kinetex 1.7 µm Biphenyl
Dimensions: 50 x 2.1 mm
Part No.: Kinetex F5: 00B-4722-AN
Kinetex Biphenyl: 00B-4628-AN
Mobile Phase: A: 20 mM Potassium phosphate (pH 2.5)
B: Methanol
Gradient: Time (min) % B
0
5
8
90
Flow Rate: 0.5 mL/min
Temperature: 30 º C
Detection: UV @ 210 nm (ambient)
Sample: 1. Fluvoxamine
Phenomenex
l
WEB: www.phenomenex.com
6
7
min
Protect any
UHPLC Column
Core-Shell Technology
Protect your column, including Kinetex coreshell columns, from damaging contaminants
and microparticulates with the SecurityGuard
ULTRA guard cartridge system!
UHPLC Column Protection
• Simple to use
• Extend column lifetime
• Pressure rated to 20,000 psi (1,378 bar)
• Fits virtually all manufacturers’ columns 2.1 to 4.6 mm ID
From Injection
SecurityGuard ULTRA Guard Cartridge System
(Cartridge connected to Holder)
Cartridge
Holder
SecurityGuard™ ULTRA
Frit
UHPLC Column to Detector
Material Chamber
}
Compounds
of Interest
Contaminants
and Particulates
See it in action:
www.phenomenex.com/SecurityGuardULTRA
Phenomenex
l
WEB: www.phenomenex.com
Core-Shell Technology
Kinetex Phase Selection
Phase
Best Use
pH Stability
EVO C18
Robust reversed phase methods even in alkaline conditions with improved
peak shape for polar basic compounds
1 - 12
C18
All purpose phase that offers the hydrophobic retention and methylene
selectivity chromatographers expect from a C18 column
1.5 - 8.5*
XB-C18
C18 phase with protective butyl side chains for improved peak shape for
basic compounds under neutral and acidic conditions
1.5 - 8.5*
C8
USP L7 phase that provides less hydrophobic and methylene selectivity
than a C18
1.5 - 8.5*
F5
Highly reproducible pentafluorophenyl propyl phase that offers a unique
combination of polar, hydrophobic, aromatic, and shape selectivity
1.5 - 8.5
Biphenyl
100% aqueous stable and allows for excellent reversed phase retention
and enhanced polar and aromatic selectivity
1.5 - 8.5*
phase chemistry that allows for greater retention and separation
Phenyl-Hexyl Reversed
of aromatic hydrocarbons
HILIC
Unbonded silica phase for HILIC conditions to provide selectivity for polar
compounds
1.5 - 8.5*
2.0 - 7.5
*pH stability under gradient conditions. pH stability is 1.5-10 under isocratic conditions.
Ordering Information
50 x 2.1
100 x 2.1
150 x 2.1
SecurityGuard™
ULTRA Cartridges‡
3/pk
––
00B-4726-AN
00D-4726-AN
00F-4726-AN
AJ0-9298
––
––
00A-4498-AN
00A-4475-AN
00A-4499-AN
00A-4474-AN
––
00B-4722-AN
00B-4628-AN
00B-4498-AN
00B-4475-AN
00B-4499-AN
00B-4474-AN
00B-4500-AN
00D-4722-AN
00D-4628-AN
00D-4498-AN
00D-4475-AN
00D-4499-AN
00D-4474-AN
00D-4500-AN
00F-4722-AN
00F-4628-AN
00F-4498-AN
00F-4475-AN
00F-4499-AN
––
00F-4500-AN
AJ0-9322
AJ0-9209
AJ0-8782
AJ0-8782
AJ0-8784
AJ0-8786
AJ0-8788
for 2.1 mm ID
1.7 μm Minibore Columns (mm)
Phases
30 x 2.1
EVO C18
F5
Biphenyl
XB-C18
C18
C8
HILIC
Phenyl-Hexyl
‡
SecurityGuard ULTRA Cartridges require holder, Part No.: AJ0-9000
If you are not completely satisfied with Kinetex
core-shell columns, send in your comparative
data to a similar product within 45 days and keep
the column for FREE.
Phenomenex
l
WEB: www.phenomenex.com
Australia
t: +61 (0)2-9428-6444
f: +61 (0)2-9428-6445
[email protected]
Austria
t: +43 (0)1-319-1301
f: +43 (0)1-319-1300
[email protected]
Belgium
t: +32 (0)2 503 4015 (French)
t: +32 (0)2 511 8666(Dutch)
f: +31 (0)30-2383749
[email protected]
Canada
t: +1 (800) 543-3681
f: +1 (310) 328-7768
[email protected]
China
t: +86 (0)20 2282-6668
f: +86 (0)20 2809-8130
[email protected]
Denmark
t: +45 4824 8048
f: +45 4810 6265
[email protected]
Finland
t: +358 (0)9 4789 0063
f: +45 4810 6265
[email protected]
France
t: +33 (0)1 30 09 21 10
f: +33 (0)1 30 09 21 11
[email protected]
Germany
t: +49 (0)6021-58830-0
f: +49 (0)6021-58830-11
[email protected]
India
t: +91 (0)40-3012 2400
f: +91 (0)40-3012 2411
[email protected]
Ireland
t: +
353 (0)1 247 5405
f: +44 1625-501796
[email protected]
Italy
Norway
t: +47 810 02 005
f: +45 4810 6265
[email protected]
Puerto Rico
t: +1 (800) 541-HPLC
f: +1 (310) 328-7768
[email protected]
Spain
t: +34 91-413-8613
f: +34 91-413-2290
[email protected]
Sweden
t: +39 051 6327511
f: +39 051 6327555
t: +46 (0)8 611 6950
f: +45 4810 6265
Luxembourg
t: +31 (0)30-2418700
f: +31 (0)30-2383749
[email protected]
United Kingdom
t: +44 (0)1625-501367
f: +44 (0)1625-501796
[email protected]
[email protected]
Mexico
t: 001-800-844-5226
f: 001-310-328-7768
[email protected]
[email protected]
USA
t: +1 (310) 212-0555
f: +1 (310) 328-7768
[email protected]
The Netherlands
t: +31 (0)30-2418700
f: +31 (0)30-2383749
[email protected]
All other countries
Corporate Office USA
t: +1 (310) 212-0555
f: +1 (310) 328-7768
[email protected]
New Zealand
t: +64 (0)9-4780951
f: +64 (0)9-4780952
[email protected]
www.phenomenex.com
BR39820216_I_2
Phenomenex products are available worldwide. For the distributor in your country,
contact Phenomenex USA, International Department at [email protected]
Terms and Conditions
Subject to Phenomenex Standard Terms & Conditions, which may be viewed at www.phenomenex.com/TermsAndConditions.
Trademarks
Kinetex is a registered trademark and SecurityGuard is a trademark of Phenomenex. Waters, ACQUITY, and UPLC are registered
trademarks, and BEH Technology is a trademark of Waters Corporation. Agilent is a registered trademark of Agilent Technologies,
Inc. Shimadzu and Nexera are registered trademarks of Shimadzu Corporation. JASCO is a registered trademark of JASCO,
Corporation. Fused-Core is a registered trademark of Advanced Materials Technology, Inc. API 4000 is a trademark of AB SCIEX Pte.
Ltd. AB SCIEX is being used under license. Milli-Q is a registered trademark of Merck KGaA, Darmstadt, Germany.
Disclaimer
Phenomenex is not affiliated with Advanced Materials Technology, Agilent Technologies, JASCO, or Waters Corporation.
Comparative separations may not be representative of all applications.
Kinetex EVO is patented by Phenomenex. U.S. Patent Nos. 7,563,367 and 8,658,038 and foreign counterparts.
© 2016 Phenomenex, Inc. All rights reserved.