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Archives of Biochemistry and Biophysics
Archimer, archive institutionnelle de l’Ifremer
Volume 445, Issue 1 , 1 January 2006, Pages 56-64
http://www.ifremer.fr/docelec/
http://dx.doi.org/10.1016/j.abb.2005.11.001
© 2005 Elsevier
Fucoidan a sulfated polysaccharide from brown algae is a potent
modulator of connective tissue proteolysis
Karim Sennia,*, Farida Guenichea, Alexandrine Foucault-Bertauda, Sylvie Igondjo-Tchena, Florence
Fiorettia, Sylvia Colliec-Jouaultb, Patrick Durandb, Jean Guezennecb, Gaston Godeaua,** and Didier
Letourneurc
a
Université Paris Descartes, Faculté de Chirurgie Dentaire, EA2496 Réparations et Remodelages
Oro-faciaux, Montrouge, France
b
IFREMER Nantes/Brest Plouzané, France
c
INSERM U. 698, Universités Paris VII & Paris XIII, CHU X. Bichat, Paris, France
Corresponding authors :
* [email protected]
** [email protected]
Abstract:
Fucoidans are sulfated fucosylated polymers from brown algae cell wall that exhibit some
heparin/heparan sulfate properties. We previously demonstrated that these polysaccharides were able
in vitro to stimulate dermal fibroblast proliferation and extracellular matrix deposition. Here, we
investigated the action of a 16 kDa fucoidan fraction on parameters involved in connective tissue
breakdown. This fucoidan is able to inhibit gelatinase A secretion and stromelysin 1 induction by
interleukin-1β on dermal fibroblasts in culture. Furthermore, we observed that fucoidan increases the
rate of association of MMPs with their specific inhibitors namely TIMPs. Using tissue sections of
human skin in ex vivo experiments, we evidenced that this polysaccharide was able to minimize
human leukocyte elastase activity resulting in the protection of human skin elastic fiber network
against the enzymatic proteolysis due to this serine proteinase. These results suggested that fucoidan
could be used for treating some inflammatory pathologies in which uncontrolled extracellular matrix
degradation takes place.
Keywords:
Fucoidan;
Glycosaminoglycans;
Extracellular
metalloproteinases; Leukocyte elastase; Interleukin-1β
matrix
remodeling;
Matrix
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