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Cell Proliferation Assay Kit
Non-toxic assay for cytotoxicity and proliferation
Economical
Sensitive
Easy to use
substrates
Cell
NAD
electron mediator
(reduced form)
WST-8 colourless
electron mediator
WST-8 formazan
orange color
dehydro genase
NADH
3
Orangu™
OD450
2
1.5
MTS
1
XTT
0.5
SDS
Cell Viability (%)
2.5
0
100
75
50
MMC
25
MTT
0
10
20
30
3
Cell Number (x10 ) / well
Orangu™ is more senstitive than
widely used alternatives. Varying
numbers of HeLa cells were incubated
in MEM containing FCS with Orangu™
(orange), MTS (grey), XTT (blue) or MTT
(yellow) for 2 hours. Orangu™ yields
equal or higher readings for all cell
numbers.
0
0.00001
0.0001
0.01
1
Toxicant (mmol / L)
Orangu™ can be used to readily
report changes in cell number as a
result of proliferation or cytotoxicity.
The above graph shows the effect of
increasing SDS and MMC concentration on the viability of HL60 cells.
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The Orangu™ assay is easily perfomed with three simple steps. (1) Orangu™ is added to the cells, (2) the cells are
incubated for a short period and (3) the cells are read in a plate reader at 450 nm wavelength.
SO3NN
NN
SO3-
em+
NO2
electron
mediator
H3COC
NO2
NO2
WST-8 formazan
WST-8
A
NO2
N NH
NN
SO3-
Na+
H3COC
SO3-
B
1.6
1.4
Structures of
WST-8 and WST-8 formazan.
0.3
0.25
0.2
1
OD450
OD450
1.2
0.8
0.6
0.15
0.1
0.4
0.05
0.2
0
0
0
0.5
1
1.5
2
-5
0
0.5
1
1.5
2
2.5
3
-5
[3H] - Thymidine x 10 dpm
[3H] - Thymidine x 10 dpm
Correlation between Orangu assay and [3H] - Thymidine incorporation assay for (A) HeLa and (B) HL60 cells. Cells
were incubated for 3 hours (Orangu ) and 4 hours ([3H] - Thymidine) respectively.
TM
TM
CELL
guidance systems
San Diego, CA | Cambridge, UK
www.cellgs.com
Ordering Information
Product
Cat No
Orangu™ 500 tests
Orangu™ 1000 tests
Orangu™ 3000 tests
OR01-500
OR01-1000
OR01-3000