Photosynthesis: Light Reaction PAGES 116-124 I. Light and Pigments A. Light: Visible light includes the colors of the rainbow B. Pigments: Compounds that absorb specific colors of light. Colors that are absorbed are NOT visible. ex. Markers and dyes have pigments I. Light and Pigments C. Chloroplast Pigments 1. Chlorophyll a – Pigment that absorbs the blue and red light energy of sunlight. Located in thylakoid membrane Chloroplast are green because they don’t absorb green! I. Light and Pigments C. Chloroplast Pigments 2. Chlorophyll b Absorbs lots of blues and some reds 3. Carotenoids Three pigments (Orange, Brown, and Yellow) that focus on absorbing blues and greens These colors are seen in the fall and in vegetables II. Light Reaction 1. First stage of photosynthesis 2. Main points: • Absorb light energy • Convert light energy to chemical energy: ATP and NADPH • Splits H2O to Produce H+ and electrons (e-) • Oxygen is released 3. GOAL: make ATP to power the Calvin Cycle II. Light Reaction 4. Reaction: 6CO2 + 6H2O + light →C6H12O6 (sugar)+ 6O2 III. Steps of the Light Reaction Step 1: Absorb Light a. Clusters of pigments called photosystems absorb light energy b. Photosystems are located in the thylakoid membrane ◦ Thylakoid Membrane: Flattened sacs within the chloroplast. Stacks of thylakoids are called grana. III. Steps of the Light Reaction Step 2: Move down Electron Transport Chain a. Light excites electrons from the photosystems and sends the electrons down the electron transport chain (ETC) b. ETC: made up of multiple proteins embedded in the thylakoid membrane III. Steps of the Light Reaction Step 2: Move down Electron Transport Chain c. Water splits, which donates the electrons needed to refresh the photosystem. d. This creates oxygen gas which diffuses out of cell e. Electrons moving down chain attach H+ to NADP+ to make NADPH, which will be used later on in photosynthesis III. Steps of the Light Reaction Step 3: Chemiosmosis a. A. Makes ATP, will be used later in photosynthesis b. B. H+ moves through ATP synthase to make ATP Light Reaction Summary Reaction Light Reaction Location Thylakoid Membrane Reactants Sunlight H2O (Water) Products O2 (oxygen) ATP NADPH
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