Indo American Journal of Pharmaceutical Research, 2014
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ISSN NO: 2231-6876
INDO AMERICAN
JOURNAL OF
PHARMACEUTICAL
RESEARCH
ADSORPTION OF METHYLENE BLUE SOLUTION BY HEAVY KAOLIN
*1
Mrs. Lata. C. Potey, 2Ms. Rashmi K. Tawari, 3 Mrs. Rajeshwari S. Sarode, 4 Mr. Mahesh A. Hadke, 5 Ms. Varsha B. Modak
Hi-tech College of Pharmacy, padoli phata, Nagpur high way, at Morwa, Chandrapur, Maharashtra (India)
2
Roselabs Bioscience Ltd. Ahmedabad
1
ARTICLE INFO
Article history
Received 16/02/2014
Available online
30/03/2014
ABSTRACT
Heavy Kaolin is a native hydrated aluminium silicate which is freed from most of impurities
by elutriation and dried. It does not contain any dispersing agent. Heavy kaolin used as
Pharmaceutical aid, adsorbent in many Pharmaceutical preparations, in poisoning to adsorb
toxin and its adsorption property has been checked in methylene blue solution in which heavy
kaolin can adsorb color of methylene blue on its surface.
Keywords
Heavy Kaolin,
Aluminium Silicate,
Pharmaceutical Aid,
Adsorb, Methylene Blue.
Copy right © 2014 This is an Open Access article distributed under the terms of the Indo American journal of Pharmaceutical
Research, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Please cite this article in press as Mrs. Lata. C. Potey, et al. Adsorption of Methylene Blue Solution by Heavy Kaolin. Indo
American Journal of Pharm Research.2014:4(02).
1588
Corresponding author
Mrs. Lata. C. Potey,
Assistant Professor,
Hi-tech College of Pharmacy,padoli phata,
Nagpur high way, at Morwa,
Chandrapur,Maharashtra (India)
[email protected]
9822711804
Vol 4, Issue 02, 2014.
Suddhasatwa Banerjee et. al.
ISSN NO: 2231-6876
INTRODUCTION
Kaolin is mainly used as adsorbent in treatment of food and alkaloidal poisoning because it can adsorb toxins. It finds use in
mixtures which are intended for dysentery, diarrhoea and symptomatic treatment of colitis, cholera. It find use in dusting powder,
cosmetic preparation etc.
Chemical formula- Al2O3.2SiO2.2H2O
PropertiesIt is light, white powder free from gritty particles, odourless, tasteless.Preparation- It is prepared from natural clay by
powdering and separating particles by electrical sedimentation.
Identification test- To 0.5g. in a metal crucible add 1g. of potassium nitrate and 3g. of anhydrous sodium carbonate, heat the
mixture untill it melt and allow to cool. To the residue add 20ml. of boiling water, mix, filter and wash residue with 50ml. of water,
filter. To the filtrate add 1ml. of 10M. Sodium hydroxide and filter to the filtrate add 3ml. of ammonium chloride. A gelatinous, white
precipitate is produced.
Uses:
Kaolin has traditionally been used internally to control diarrhea. Kaolin has also been used topically as an emollient and
drying agent. Specifically, it has been used to dry oozing and weeping poison ivy, poison oak, and poison sumac lesions. It has also
been used as a Laboratory Testing: Kaolin has been recognized as a coagulation activator and has been incorporated into various
laboratories testing to measure ACT. These measurements are imperative during cardiopulmonary procedures in order to guide
heparin anticoagulation to prevent thrombosis and reduce inflammation.
Additionally, kaolin has been studied for its effects when testing horse serum for seroconversion against equine influenza
virus, which causes a major respiratory disease among horses. The hem agglutination-inhibition assay is very rapid and has a
reproducibility of 84% to 96%. However, this test is affected by the presence of nonspecific inhibitors found in the horse serum. Thus,
kaolin was studied to determine its ability to remove these nonspecific inhibitors. When testing for antibodies against equine influenza
virus subtype 1 (H7N7), false-positive results occurred. However, when testing against antibodies for equine influenza virus subtype 2
(H3N8), false-positive results did not occur. Additionally, titers of sera treated with kaolin were similar to those that were untreated,
indicating that kaolin was not effective in removing nonspecific inhibitors from equine serum.
One small study suggested that the addition of kaolin to oil field wastewater can result in removal of chemical oxygen demand,
removal of scaling ions, such as iron, calcium, and magnesium, improvement in membrane filter index, bactericidal effects, and
inhibition of corrosion. Protectant for the temporary relief of anorectal itching and diaper rash.
MATERIAL AND METHODS
Chemicals and Reagents:
Heavy kaolin, Methylene blue, is from Loba Chem Pvt. Limited, Mumbai. All solvents were distilled before use and dried
whenever required. Distilled water was used to make up volume.
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1589
Experimental procedure.
Prepared 0.37%w/v solution of methylene blue and 0.003%w/v of methylene blue solution as a standard prepared by
pipetting out of 1ml. from 0.37%w/v solution of methylene blue solution.1.0 g. of heavy kaolin weighed and taken in centrifuged tube
and 10 ml. of 0.37 % w/v methylene blue solution was added then allow to settle, centrifuged, kept aside for adsorption. In practice
complete adsorption was checked every after half an hour by withdrawing 1 ml. of upper layer and diluted to 100 ml. i.e. 0.003%w/v
(sample) and color was compared with standard 0.003%w/v methylene blue solution
Fig. 1 - Adsorption of methylene blue on surface of heavy kaolin after 2.5 hours.
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Vol 4, Issue 02, 2014.
Suddhasatwa Banerjee et. al.
ISSN NO: 2231-6876
RESULT AND DISCUSION
In practice after every half an hours, 1 ml. of test solution was pipette out and diluted to 100 ml. with distilled water (0.003
%w/v) and compared with standard methylene blue solution. After 2.5 hours it was seen that color of test solution was intensely less
colored than standard solution. It was concluded that heavy kaolin can adsorb elegantly on it surface and can be used in
pharmaceutical preparations.
In future we can compare the adsorption property of heavy kaolin with other adsorbent.
Fig. 2- Color comparison showing between sample and standard.
REFERENCES
1. Indian Pharmacopoeia, government of India ministry of health and family welfare, 2010, volume-I1, I.P. Commission Ghaziabad,
1537.
2. G. R. Chatwal, Pharmaceutical Chemistry Inorganic, volume-I, Himalaya publication, 357.
3. Pham Till Hang and G. W. Brindley, “Methylene blue absorption by clay minerals. determination of surface areas and cation
exchange capacities (clay-organic studies xviii)” Clays and Clay Minerals, 1970, Vol. 18, pp. 203-212. Pergamon Press. Printed in
Great Britain.
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