Table1
accession no.
Full-length
cDNAs
AK060270
AK073146
OsBt1
1749 500
1952 500
54.8
54.8
9
9
10
10
AP004459
AP004459
AP004459
AP004459
OsBt2
581
317
1930
1749
85
51
514
479
9.1
6.2
56.1
56.1
8
8
8
8
10
4
2
10
AP004382
AP004382
AP004317
AP120988
AP004382
OsAGPL1
OsAGPL2
OsAGPL3
OsAGPL4
2067
624
2382
2313
2132
511
86
518
519
509
55.4
9.2
57.5
57.6
55.8
3
3
1
5
7
15
3
17
15
16
AK071826
AK103906
AC091687
AC091687
AC091687
AC091687
AK069296
AK105092
subunit AK071497
AK100910
AK121036
AC096689
AC096689
AC007858
AP003574
(bp)
Chr. a） Exon No. b)
(kDa)
PAC
AP004756
AP004756
AK058246
Calc MW
(aa)
BAC
AP004011
AP004011
Small subunit AK058551
Large
length
gene name
a) chromosome number on which each AGPases gene is located
b) The number of exons
c) subcellular localization in the cell of four large subunit proteins are predicted by PSORT program (http://psort.nibb.ac.jp/)
The clone which is amplifeid by RT-PCR with specific-primers pairs-sets is indicated in bold.
localization
Plastid
(Sikka et al., 2001)
Cytoplasm
(Anderson et al., 1989)
mitochondria c)
Cytoplasm c)
Cytoplasm c)
Chloroplast c)
Table1
accession no.
Large subunit
MW
localization
PAC
AP004756
AP004459
(aa)
(kDa)
OsAPS1
OsAPS2
BAC
AP004011
AC091687
1952 500
1749 479
54.8
56.1
9
8
10
10
Plastid (Sikka et al., 2001)
Cytoplasm (Anderson et al., 1989)
OsAGPL1
OsAGPL2
OsAGPL3
OsAGPL4
AK069296
AK071497
AK100910
AK121036
AC096689
AC007858
AP003574
AP004382
AP004317
AP120988
AP004382
2067
2382
2313
2132
55.4
57.5
57.6
55.8
3
1
5
7
15
17
15
16
Cytoplasm c)
Cytoplasm c)
Chloroplast c)
gene name
Small subunit
length
Full-length
cDNAs
AK073146
AK103906
(bp)
511
518
519
509
Chr. a） Exon No. b)
a)Chromosome number on which each AGPases gene is located
b)The number of exons
c) Subcellular localization in the cell of four large subunit proteins are predicted by PSORT program (http://psort.nibb.ac.jp/)
Fig 1
I II III
IV
200bp
position length
(nt)
(bp)
OsBt1
1686-1924
239
OsBt2
19-268
250
OsAGPL1
1519-1740
222
OsAGPL2
2129-2355
227
OsAGPL3
1873-2214
341
OsAGPL4
1728-2024
297
Fig 3
(B)
(A)
OsBt1
OsBt2
OsAGPL1
OsAGPL2
OsAGPL3
OsAGPL4
OsGBSSII
rRNA
D
Relative amounts of transcripts (%)
L
1
100
0.8
80
0.6
60
0.4
40
0.2
20
00
1L
D
2
Leaves
OsBt1
3
L4
D5
Leaves
OsBt2
6
L
7
D8
Stems
OsAGPL3
Fig 4
A
B
Sucrose
0
1.5
3
6
Glucose
10 (%)
0
OsBt1
OsAGPL3
OsBt2
rRNA
OsAGPL1
OsAGPL2
C
OsAGPL3
OsAGPL4
OsAGPL3
rRNA
rRNA
1.5
3
6
10 (%)
Fig 5
A
Sugar-free
Sucrose
Glucose
6
3
starch content
(mg / g DW)
B
(%)
10
120
100
80
60
40
20
0
3
6
Sucrose
10
3
6
10 (%)
Glucose
OsBt1
OsBt2
OsAGPL1
OsAGPL2
OsAGPL3
OsAGPL4
rRNA
+ABA
+BA
+2.4-D
Free
Fig 6
ABA
OsAGPL3
rRNA
Control
Fig 7
- Suc + ABA
0
1
10 50 100 0
+ Suc + ABA
1 10 50 100 (mM)
Fig 8
120
(mg / g DW)
starch content
100
80
60
40
20
0
control
+ABA
+Suc
+Suc+ABA