Cell Organelles Chapter 4 II Part Cell walls • • The exterior surface cells of some plants, mushrooms and some protists have cell walls. Cell walls are made of polysaccarides like cellulose and quitin. – The cell walls support and protect the cells. Since they are porous and fragile. Cell walls • Cell walls can have several layers: – The primary cell wall is in the extreme exterior. – The secondary cell walls are in the middle between the primary cell wall and the plasma membrane. – The primary cell walls bond to each other by intermediate lamella. Plant cells Cytoskeleton • The cytoskeleton forms a proteic fiber net inside the cytoplasm. – It is composed of microtubules (thinner), intermediate filaments and microfilaments (thicker). Cytoskeleton Cytoskeleton Cytoskeleton • Main functions: – Cell shape. – Cell movement (examples: sperm cells or contracting muscle cells). – Organelle movement during endocytosis (when large particles are engulfed by the plasma membrane and pull the vesicles into the cell) and exocytosis (vesicles budded off the endoplasmic reticulum or Golgi apparatus). – Cell division. In eukaryotic cells microtubules move the chromosomes and separate them. In animal cell division help the centrioles move. Cilia and flagella • They both are thin extensions of the plasma membrane. • They both surge from a basal body. Basal bodies surge from centrioles. • Cilia are numerous and smaller (10-25 microns). • Flagella are unique and longer (50-75 microns). Cilia and flagella Cilia and flagella • Microtubules slide together (using ATP), creating cillia and flagella´s movement. • Functions: – Cilia and flagella can move the cell or create liquid currents arround the cell. – Cilia rows resembling the arm of a swimmer doing the breast stroke. (movement-cleaning) – Flagella moves in a wavelike motion and helps straight ahead movement. Nucleus • The nucleus has 3 main parts: – Nuclear envelope – Cromatin – Nucleolus Nucleus • The nuclear envelope separates chromosomes from the cytoplasm. – It consists of a double membrane that has nuclear pores to transport organic and inorganic molecules (water, ions, proteins, ribosome pieces and RNA). – The exterior nuclear membrane has ribosomes attached to it. Nucleus Nucleus Nucleus • The nucleus has DNA in different presentations: – As chromosomes easily seen with microscope light. Are composed of genes and provide the molecular code for a huge variety of proteins. – As cromatin directs DNA´s reactions with RNA messenger to synthesize proteins. Chromosomes and cromatin Nucleus • The region inside the nucleus that is usually seen in a darker color is called the nucleolus. – The nucleolus is the place where ribosomes are synthesized. – Ribosomes synthesize proteins (amino acids). Ribosomes Membrane system • The membrane system includes the plasma membrane and other organelle membranes. • Examples of organelles that have membranes: nucleus, endoplasmic reticulum, Golgi apparatus, lysosomes, vesicles and vacuoles). Membrane system • The plasma membrane isolates the cell and allows the transport regulation. Membrane system • The vesicles transport the membranes and its content between the separated regions of the membrane system (transport capsules). • The ER forms channels inside the cytoplasm. • There are 2 kinds of ER: – smooth – rough Endoplasmic reticulum ribosomes vesicles Endoplasmic reticulum Membrane system • The Smooth Endoplasmic Reticulum (SER) has no ribosomes. • Functions: • It has enzymes that eliminate the toxicity of certain drugs (in the liver). • It manufactures large quantities of lipids such as steroid hormones made from cholesterol. Ex: sex hormones in mammalian reproductive organs. • It synthesizes lipids. • It stores calcium ions. vesicles Smooth endoplasmic reticulum Membrane system • The Rough Endoplasmic Reticulum (RER) has ribosomes attached to its exterior. Functions: – It produces proteins like digestive enzymes and hormones. – For example insulin (hormone that helps metabolic utilization of nutrients, mainly with carbs) Lack of it causes diabetes and too much insuline causes hypoglycemia. – It produces all the components of new membranes. Rough endoplasmic reticulum Membrane system • The Golgi apparatus is a stack of flattened that are connected to each other. • Functions: – It gets proteins from the ER through the vesicles and classifies them. – It modifies certain molecules. Example: proteins into glycoproteins. Glycoproteins are composed of 1 protein and carbohydrates. – It packs material into vesicles to export it. Golgi apparatus The ER sends vesicles with proteins to the Golgi apparatus. Golgi apparatus The modified proteins leave the Golgi apparatus inside other vesicles. Membrane system • What happens with substances that are produced by the membrane system? 1. Option 1: The proteins that are secreted by the RER, travle through the Golgi apparatus, and are then exported by the plasma membrane. – On Figure 4-14 you can watch the process by which antibodies (proteins produced by white globules) attach to strange organisms and destroy them. FIGURA 4-14 How a protein is synthesized and exported? Membrane system 2. Option 2: The digestive proteins produced by the RER, travel through the Golgi apparatus and are packed like lisosomes to be used by the cell. • The lisosomes mix with the food vacuoles and digest the food into basic nutrients. FIGURA 4-15 Lisosome and food vacuole synthesis and fusion. Memebrane system 3. Option 3: The proteins and lipids that are produced in the ER flow through the Golgi apparatusand enlarge the plasma membranes and the organelle membranes. Vacuoles They are cellular membrane bags that contain certain fluids. • Functions: – The sweet water organism´s contractile vacuoles drain water. Contractile vacuoles Vacuoles • Functions: – The plant central vacuoles: • They participate in the hydric equilibrium in the cell. • They store wastes, nutrients or toxic pigments. • They provide the turgent or turgor pressure in the cytoplasm to keep the cell rigid. Mitochondria • The mitochondrias are rounded, oval or tubular organelles that have a pair of membranes. – The interior membrane forms deep folds called cristae. – The intermembrane compartment is located between the outer and inner membranes. – The matrix is found between the interior membrane folds. Outer membrane Inner membrane Intermembrane compartment matrix cristae Mitochondria Mitochondria • The endosymbiotic hypothesis states that mitochondria could have evolved from prokaryotic bacteria. Mitochondria • They work like power plants inside the cell. – Mitochondria extract energy from the food molecules. – This energy is stored like ATP. – The process of energy extraction implies anaerobic and aerobic reactions. Chloroplasts • The chloroplasts are specialized organelles surrounded by a double membrane: - The outer membrane. – The inner membrane; which locks a liquid called stroma. • The bunch of membrane empty and interconnected sacks (granum) are inside the stroma. They are called thylakoids. Chloroplast Outer membrane Inner membrane stroma thylakoid Thylakoid interconnect ing channel Granum 1 micra Chloroplast Chloroplasts • The tilacoid membranes contain chlorophil and other light-capturing pigments that capture the Sun light and synthesize sugar from CO2 and water (fotosynthesis). Outer membrane Inner membrane stroma thylakoid Channel Granum Chloroplast Plastids • Plastids are found only in plants and photosynthetic protists. • They are surrounded by a double membrane. Plastids • Functions: – They store starch (synthesized from sugars that were produced during photosynthesis). – They store the molecules from the pigments that give fruits their color. Plastid What are the major features of prokaryotic cells? – They are very small < 5 microns long. – They have specialized surface features. – They have less specialized structures in their cytoplasm. – They usually have a rigid cell wall. Prokaryotic cells Chromosome Cell wall Plasma membrane ribosomes capsule Prokaryotic cells Prokaryotic cell • • • They do not have a defined nucleus and many other organelles. Some of them move because they have a flagella. Some common shapes are: rod, sphere or helix. Prokaryotic cells • Bacteria that infect other organisms, posses surface features that help them stick to specific hot tissues. • Examples: capsules, slime layers and pili (made of polyssacharides). Prokaryotic cells Prokaryotic cells • They have only 1 chromosome composed of one long DNA fiber. – This chromosome is located in the central region of the cell (nucleoid region). • They also have small DNA rings called plasmids (cytoplasm). Prokaryotic cells • • Some photosynthetic bacteria have internal membranes with light-capturing proteins and enzymes that catalyze the synthesis of high-energy molecules. Bacteria cytoplasm may contain ribosomes (contain proteins) and food granules that store energy-rich molecules. Prokaryotic cell Photosynthetic membrane
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