Mode of action Parasitic nematodes to tackle the larvae and pupae of the black vine weevil (Otiorhynchus sulcatus) After application, the parasitic nematodes actively Larvanem (Heterorhabiditis bacteriophora) Entonem (Steinernema feltiae) • Minimum soil temperature of 14°C • Minimum soil temperature 8°C nematodes then feed on the contents of the larvae, • Effective in combating larvae and pupae • Effective in combating larvae • Release period: early autumn and late spring • Release period: late autumn and early spring • Dosage 500 million per 1,000 square metres • Dosage 500 million per 1,000 square metres hunt for the larvae and penetrate them. The releasing certain bacteria from their intestinal tract. These bacteria convert tissue from the host into substances that are easy for the nematodes to absorb. The larvae die within a few days. The population development of the nematodes within the host population is dependent on the number (dosage) applied and the size of the host population. In this regard, it is important that Larvanem and Entonem be applied on uncovered, moist soil. “Life cycle and moment of application of nematodes (beneficial nematodes)” Stage January February March April May June July August September October November COMBATING THE BLACK VINE WEEVIL Combating the black vine weevil quickly and effectively December Larva Pupa Egg www.koppert.com Adult Application of Entonem (S. feltiae) Application of Larvanem (H. bacteriophora) Period of presence Application method Open ground Container cultivation Spraying 0.5 million nematodes per litre 1 L liquid/m² 0.5 million nematodes per litre 1 L liquid/m² • Apply to moist uncovered soil 0.5 million nematodes per litre 1 L liquid/m² • Open ground Pouring Quantity of liquid Application 100 ml per 10 litres of container volume › Spray/use sprinkler, trickle irrigation • Container cultivation › Spray/use sprinkler, trickle irrigation, watering can rose Combating 3rd stage larvae of the black vine weevil, 1 week after treatment Entonem (S. feltiae) Advantages of using parasitic nematodes Larvanem (H. bacteriophora) • Safe application for user 100% • Fast and effective • Good persistence • Can be used in tree nursery crops (yew, thuja, rhododendron), berry cultivation, strawberries, 80% Mortality rate ornamental crops and various pot plants, etc. Package options 60% › 100 square metres • 500 million › 1,000 square metres • 50 million COMBATING THE BLACK VINE WEEVIL Dosage 40% Comments • Do not cover exterior/bark entry into the soil 20% › obstructs • Prevent occurrence of dry spots (the formation of raised spots during trickle 0% 5˚C 8˚C 12˚C 15˚C 20˚C Temperature irrigation) › black vine weevils seek out drier spots, while parasitic nematodes prefer moist soil • Check the ends of the trickle irrigation • Mix continuously in order to prevent Equipment • Watering can, sprinkler, backpack spray unit • Remove all strainers to prevent blockages • Spray nozzle opening: at least ½ mm • Water the crop (with a sprinkler) before evenly distributed over the whole system. • If necessary, repeat the treatment during the development of the larvae (autumn and spring). and after application • Keep soil sufficiently moist for the first (500 microns) • Pressure: max. 12 bar WWW.KOPPERT.COM • Spray over soil surface hoses to verify that the nematodes are | [email protected] two weeks after application PR21121/110301 www.koppert.com or vehicle-mounted spray unit, trickle irrigation nematodes sinking to the bottom
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