Figure S2
Experiment 1
Experiment 2
chlorophyll density
chlorophyll density
0.035
0.020
0.015
0.010
0.005
0.000
0
1
2
3
4
5
6
0.030
wild-type
psr1
psr1snrk2.2
psr1snrk2.1snrk2.2
0.04
0.03
0.02
0.01
wild-type
psr1
psr1snrk2.2 (D3)
psr1snrk2.2 (N1)
psr1snrk2.1snrk2.2
0.025
0.020
0.015
0.010
0.005
0.000
0.00
7
chlorophyll (mg/mL)
chlorophyll (mg/mL)
chlorophyll (mg/mL)
0.025
chlorophyll density
0.05
wild-type
psr1
psr1snrk2.2
0.030
Experiment 3
0
24
48
72
time (days)
96
120
144
0
168
24
48
viability
96
120
144
168
viability
120
wild-type
psr1
psr1snrk2.2
psr1snrk2.1snrk2.2
100
80
60
40
20
% unstained (live) cells
120
% unstained (live) cells
72
time (h)
time (h)
wild-type
psr1
psr1snrk2.2 (D3)
psr1snrk2.2 (N1)
psr1snrk2.1snrk2.2
100
80
60
40
20
0
0
0
24
48
72
96
time (h)
120
144
168
0
24
48
72
96
120
144
168
time (h)
Figure S2. Chlorophyll content and cell viability in –P cultures. The top three panels show time courses of chlorophyll concentration
per unit culture volume in wild-type cells and mutants grown in TA (Pi-free, no glucose-1-phosphate) liquid medium for 7 d. Three
independent experiments are shown. In each experiment, chlorophyll concentrations are the average of duplicate measurements, plus or
minus the standard deviation. Cultures were inoculated to approximately equal initial chlorophyll concentrations in experiments 1 and 2,
while experiment 3 cultures were inoculated to give approximately equal initial cell density. The two bottom panels show time courses of
viability of the strains in experiments 2 and 3. Non-viable cells were distinguished from viable cells by staining with 0.25% Evans Blue dye.
Percentage viability is calculated from the average of 2 to 4 measurements, plus or minus the standard deviation. For all experiments the
wild-type strain was CC-1690, the psr1 mutant was psr1-1 (A4), the triple mutant was psr1snrk2.1snrk2.2 (A2); in experiments 1 and 2 the
psr1snrk2.2 (D3) strain was used (see Table 1).