History of Varian
HPLC
Frankie Button
HPLC Instrument Manager, Europe Region 1
November 2007
1966 is where it all started……..
1966 – LC Research and Development Group of Varian
was formed
LC Development and Innovation took place every 1 to 3
years.
In the early years this was mainly by product
development
In later years this has been mainly by acquisition
History of Varian HPLC Products
1967 – Model 4000 Gas Displacement Pump and
Micro-adsorption detector (MAD)
History of Varian HPLC Products
1968 – Varian purchased LC Division of Picker-Nuclear
(Dedicated Nucleotide Analyzer, LCS-1000, 254 nm UV
detector.
1971 – Model 4200 Gradient Elution Chromatograph
(syringe pump), Variscan Scanning UV Detector
(Techtron – Melbourne)
History of Varian HPLC Products
1974 - Model 8500 HPLC syringe pump, RI detector,
8000 AutoSampler for HPLC, CDS 101
History of Varian HPLC Products
1976 – Varichrom Variable Wavelength UV-Vis detector
(Cary)
2nd Generation CDS System, with system control!
History of Varian HPLC Products
1977 – Fluorichrom filter fluorimeter
History of Varian HPLC Products
5000 series HPLC pump, major step forward
• 5010 Isocratic analog pump
• 5020 Binary gradient computer controller
• 5030 Isocratic computer controller
UV-1 254 detector
History of Varian HPLC Products
Inside the 5020
History of Varian HPLC Products
1979 – UV-50 UV-Vis detector
1980 – vista 54/56 HPLC (Controlled by Vista 401
chromatography data station) 5060 Ternary gradient
HPLC pump
History of Varian HPLC Products
1981 – Post Column Reactor, RI-3 (refractive index
detector from LDC)
1982 – UV-100 time programmable UV-Vis detector with
fast HPLC option!
1983 – 5560 HPLC, scanning UV-200 detector,
8085 integrated AutoSampler, CDS-402 for control
History of Varian HPLC Products
1984 – 9090 low loss variable volume AutoSampler,
2010 Isocratic pump, 2020 Gradient controller, 2050
Programmable UV-Vis detector, 2070 Monochrometer
based Fluorescence detector (all from Jasco)
History of Varian HPLC Products
1985 – PolyChrom 9060 Diode Array Detector, AASP
Automated solid phase extraction system
( Online SPE!!!)
History of Varian HPLC Products
1986 – CM-1 conductivity detector (OEM), DS-604 GPC
calculation software
1987 – RI-4 (OEM from ERC in Japan), 2500 Isocratic
pump, 2502 Isocratic pump and gradient controller,
2550 time programmable UV-Vis detector (Jasco)
History of Varian HPLC Products
1988 – Star 9010 ternary gradient pump, 9050 time programmable UVVis detector, 9095 High Performance AutoSampler, 9065 Diode Array
detector, 9020 Windows based HPLC workstation
History of Varian HPLC Products
1989 – LC Workstation Rev C, SP-99 column oven
(Spark Holland)
1990 – 3D View for PolyView, PCR carbamate system
(Pickering), 9001 Isocratic pump, Marathon
AutoSampler (Spark Holland)
1991 – 9070 Fluorescence Detector (Linear, Spectra
Physics, Thermo), 9100 Cooled AutoSampler
1992 – MCA for PolyView
1993 – 9012/9002 extended flow range pumps, Mistral
oven (Spark Holland), Electric 9100
History of Varian HPLC Products
1994 – Workstation version 4.0 (LC and GC combined),
PolyView 4.0 (IsoView), 9300 AutoSampler (Spark
Holland), Prospekt Automated solid phase extraction
(Spark Holland)
History of Varian HPLC Products
1995 – Star 9080 Electrochemical detector ( Antec),
Star 9040 RI detector (ERC), Star control of 9200 and
9300
1996 – 9025 Automation panel, LC/NMR, 9012
Quaternary pump, 9075 fluorescence detector (Thermo)
1997 – Varian buys Rainin – SD-200/300, SD-1, UV-1,
UV-C, UV-D, PDA-2, AI-1A, AI-96, AI-03, RamPak,
ISCO FC-1, ISCO, FC-4
History of Varian HPLC Products
1998 – ProStar product line ( combines Rainin and
Varian products), ProStar 330 Photodiode Array
(Hitachi) PolyView 2000
1999 – ProStar 500 CVM, Helix system
2000 – ProStar 363 Fluorescence detector, Varian buys
Van Kel, Varian buys R&S, 520 Oven
History of Varian HPLC Products
2001 – PrepStar SD-2, PrepStar 530, Responder
software, Helix 364 Fluorescence software, Star
Reviewer for dHPLC, Polaris Educational systems
2003 – ProStar 325 UV/Vis detector
Key technical advances for Varian HPLC
Variscan - scanning UV-Vis detector 1971
5000 pump – single piston single pump ternary gradient system
1978
Vista 401 – 4 channel, control LC and GC, dual high speed
printers, replot with baseline, raw data storage 1980
UV 100 – No RI high performance analytical product 1982
PolyChrom 9060 PDA with mathematical evaluation of purity –
1985
Star Workstation – Windows based data system, 1988
PolyView spectral processing software – 1988 (MCA 1992)
SD-1 – the Waters alliance before there was an alliance – 1991
with Rainin. (A fire truck without the wheels)
ProStar 500 CVM – The ultimate in valving capability – 1999
Varian products that lasted
5000 pump deign – now in ProStar 230
Vista 401 capabilities – now in Star and Galaxie
UV-100 design – Obsolete in 2003 by ProStar 325
Spectral processing software – never surpassed!
Products that customers loved
LC 5000 – many are still working today
Star 9010/9012/230 – same as above
Vista 401 – Incredible innovation and very easy to use
UV-100/9050/ProStar 310 – they were the workhorses
of the lab
SD-1 – Unparalleled Performance
SD-200/SD-300/210/218 – the best in world for
analytical to small scale prep
HPLC at Varian for over 40 Years
Varian have always been, and always will be an
Innovator in HPLC
Even in the last 10 years we have produced product
after product, and several acquisitions
Over $8M Invested in the last 4 years to put us back on
the HPLC Map
This new Era starts with, because it’s the first of many
NEW HPLC products:
The Future………..
iHPLC
Concept of the iHPLC
• 1st Fully Integrated HPLC System
• Easy to use, install and MAINTAIN
• Compact and Stylish in Design
• Complementary to part of existing modular line
• A Major step forward in performance and ergonomics
and User Experience
• Single part numbers, easier to sell
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iHPLC
Two New Models
Analytical
Analytical to Prep
iHPLC Analytical
Concept:
• One Power Button
• One Power Cable
• One Communication cable
• …Whilst retaining the Self maintenance features
• Pre-plumbed and “Ready” out of the box
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• Sample tray accessible without opening doors
iHPLC Analytical
USP:
• High Performance Analytical System
• Can be Optimised easily for good Fast LC performance
• The “First COMPLETE Integrated Solution”
• Simplicity in a one Box
• Simple to Install…Simple to Use....Simple to Maintain
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• Neat, Compact and Stylish product
iHPLC Analytical
Consists of:
• NEW Pumps
• NEW Autosampler
• NEW Detectors
• Existing UV/DAD Detectors in an Integrated format
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iHPLC Analytical
USP:
• The Only complete range of High Performance
Information Rich LC Detectors
• IMS Software for simple user diagnostics, OQ and
Service
• Storage space for LC Columns, Fittings and Tubing
• Tray Space for Columns, manual, tools, Software CD
etc…
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iHPLC Analytical to Prep
Consists of:
• Existing 210/218 Pumps in several integrated
configurations
• NEW Fraction Collector
• NEW Prep Autosampler
• NEW Module for Column and Solvent Selection
• Existing UV/DAD Detectors in an Integrated format
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iHPLC Analytical to Prep
Concept:
• One Power Button/Cable
• One Communication cable
• Easy interchangeability of components to retain
flexibility
• Pre-plumbed and “Ready” out of the box
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iHPLC Analytical to Prep
USP:
• High Performance Analytical System
• High Performance “Analytical to Prep” System
• High Performance Prep System
• The “Only COMPLETE Integrated Solution”
• Accepted Hardware
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iHPLC Analytical to Prep
USP:
• Simple to Install…Simple to Use....Simple to Maintain
• Neat, Compact and Stylish product
• Modern, Stylish Fraction Collector…That works!
• Simple FC programming and manual intervention
• New Switching module concept, stylish design
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Galaxie Driver
• Awesome interface
• Very “Easy on the eye” –shockwave technology
• Simple to use
• An enormous step forward
• Installation configurator – simple to install
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Other Highlights
• Solvent tray capacity
• “Ski Slopes” for sample tray insertion/removal
• Single waste port
• Easy access for servicing/tubing and fitting
replacement
• Leak detector
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Other Highlights
• Modern Manual Injectors – push button
• Bottle Management
• Software Tool Tips – makes everything easy
• Online Help and Videos “ Cary Style”
• Excellent Brochures – hoorah a brochure!!!!!!!
• New Data Sheets/Price Lists and App Notes
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Positioning
• Form part of our New LC range
• Still have modular options – very important
• Rebranded XXX-LC, eliminate ProStar name
• First product 356-LC Refractive Index plus
– 210/218/325/335etc..
• New Grey “ Barbie Showers”
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Positioning
• Integrated Systems(iHPLC)
– Standard popular configurations
– New Format
Analytical
Semi-Prep
Prep
• Modular Systems(XXX-LC)
– Low cost systems
Process
EXCELLENT AND COMPREHENSIVE
– Odd configurations
RANGE OF CONSUMABLES
GPC
– Biocompatible
– Online SPE
Flash
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• Prep 2 Process/Flash LC
Turnkey
• GPC Systems
Custom
Conclusion
• Compact and Stylish Integrated Systems
• Retain flexibility and maintenance features
• Excellent hardware performance
• Excellent User Interface – pleasant experience
• Simple to Install
• Fits the topical “Fast LC” Model using our Two Brand
NEW Small Particle LC Columns
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