Supporting Information Figures S1–S9
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/Paper-submis
Seaview [blocks=10 fontsize=10 A4] on Wed May 8 16:15:41 2013
OsPupK46-2
isotig20112
1
MLLCQRASKN APRIESFLQK QETSNPKRYT LSEVKRMTKS FAHKLGRGGF GTVYKGSLPD GREIAVKMLK
---------- ---------- ---------- ---------- ---------- ---------- ----------
OsPupK46-2
isotig20112
71
DTKGDGEEFI NEVAGISKTS HINVVNLLGF SLQGSKRALI YEYMPNGSLD RYSFGDSSVQ GDNTLSWDRL
---------V NEVMSIGRTS HVNVVSLYGF CLEGSKRALI YEYMSNGSLD NYIY---SEN PKETLGWERL
141
FNIIVGIARG LEYLHCHCNI RIVHFDIKPQ NILLAQDFCP KISDFGLSKL CHLKESRISI NGLRGTPGYI
YAIAIGIARG LEYLHHSCNT RIVHFDIKPQ NILLDKDFSP KIADFGLAKL CHTKESKLSM TGARGTIGFI
OsPupK46-2
isotig20112
211
APEVFSRQYG SASSKSDVYS YGMVVLEMAG AKKNINVSTG SSSKYFPQWL YDNLDQFCCP TGEISSQTTD
APEVHSRTFG VVSTKSDVYS YGMMLLEMVG GRRNVKINCL K--------- ---------- ---------N
OsPupK46-2
isotig20112
281
LVRKMVVVGL WCIQLVPTDR PSMREVLEML ESNGRDLPLP PKGL
PVSSIFQIGF TTTLGKMMDY KHVKSQAKLR KSQERWS--- ----
OsPupK46-2
isotig20112
!
Fig. S1 Alignment of Holcus lanatus translated isotigs 20112 against an Oryza sativa
PSTOL1 (OsPupK46-2) reported as a newly identified tolerance gene for phosphorous
deficiency in Gamuyao et al. (2012).
1
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnets/
Seaview [blocks=10 fontsize=10 LETTER] on Mon Mar 18 20:32:49 2013
isotig16840
LOC_Os12g34450.1
XP_003575870.1
1
MESRPETAVA VRGLRFAGAG HRGALLLALL FAAVTAFLPV AEPSCPRDNS VVKDINQMHQ SSYGLQGLSH
M--------- ---------- ---------- ---------- ---------- ---------- ---------MGTGPTAPAA --------SG HRGSLLLALL LGAAAAFLPL AEPSCPRDNS LVKDINQMHQ SDYGRKGFSH
isotig16840
LOC_Os12g34450.1
XP_003575870.1
71
ITVAGALAHG MKEVEVWLET VSAGQRTPVH RHSCEEVFIV LKGRGTLLLG STSLPYPGTP KEIPFFQNST
---------- ---VEVCLQT FGPGQRTPIH RHSCEEVFVV LKGKGTLFLG SSSMKYPGQP QEIPVFQNST
ITIAGALAHG MREVEVWLQT FGPGQRTPIH RHSCEEVFVV LKGRGTLLLG STSLQYPGTP QEIPVFQNST
141
isotig16840
FTVPVNDPHQ VWNSDEHEDL QFLVIISRPP VKVFLYDDWS MPHTAAKLKF PFLWDEDCLA APKDEL
LOC_Os12g34450.1 FTIPVNDPHQ VWNSDEHEDL QVIVVISRPP IKVFFYDDWN MPHTAAKLQF PIFWDEECLI APKDEL
XP_003575870.1
FTVPVNDPHQ VWNSDEHGDL QVLVIISRPP VKIFTYDDWS MPHTAAKLKF PYFWDADCLP APKDEL
Fig. S2 Alignment of Holcus lanatus isotig 16840 with Oryza sativa putative auxinbinding protein 4 precursor and B. distachyon predicted auxin-binding protein 4-like.
2
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnets
Seaview [blocks=10 fontsize=10 LETTER] on Wed Mar 20 10:37:41 2013
isotig12721
XP_003559031.1
LOC_Os03g01270.1
1
---------- ---------- ---------- ---------- ---------- ---------- ---------M--------- ---------- ---------- ---------- -----ASSSS SSVSAALLLC LLLAIIPPPV
MAGRSRRRSF WSVGVAAALL CLLAAHGCSA KHHKPKPTPG GISGNASSSS SNSST----- ---PSIPPPV
isotig12721
XP_003559031.1
LOC_Os03g01270.1
71
---------- ---------- ---------- ---------- ---------- ---------- ------MTSC
A--------- ----TNGSSN SSSPGAGDEG WMDARATWYG APNGAGPDDN GGACGFKDVN LPPFSAMTSC
APTPTAPTPP IPSPGTGSSN GSS-GGGGGG WLNARATWYG APNGAGPDDN GGACGFKNVN LPPFSAMTSC
isotig12721
XP_003559031.1
LOC_Os03g01270.1
141
GNEPLFKDGK GCGSCYQIRC VAQNHPACSG VPETVIITDM NYFPVSRYHF DLSGTAFGAM AMPGRNDQLR
GNEPLFKDGK GCGSCYQIRC LSRMHPACSG VPETVIITDM NYYPVSRFHF DLSGTAFGAM AKDQRNDELR
GNEPLFKDGK GCGSCYQIRC VG--HPACSG LPETVIITDM NYYPVSLYHF DLSGTAFGAM AKDNRNDELR
isotig12721
XP_003559031.1
LOC_Os03g01270.1
211
HAGIIDMQFK RVPCQYPGLT VTFHVEHGSN PYYMAILVEY ENGDGDVDQV DIMESRPDPA AAGAEDGMAP
HAGIIDMQFK RVACQYPGLT VTFHVEHGSN PYYMAILVEY ENGDGDVDQV DIMESTPDGS G-------EP
HAGIIDIQFR RVPCQYPGLT VTFHVEQGSN PVYMAILVEY ENGDGDVVQV DLMESR---Y STGGVDG-TP
isotig12721
XP_003559031.1
LOC_Os03g01270.1
281
TGEWVPMKES WGSIWRMDTR RPMQGPFSLR ITNESGKTLV ADQVIPADWE PNQIYSSIIQ FD
TGQWVPMKES WGSIWRMDTR RPMHGPFSLR ITNESGQTLV ADQVIPADWE PNAIYSSIIQ FD
TGVWTPMRES WGSIWRLDTN HPLQGPFSLR ITNESGKTLI ADQVIPADWQ PNTVYSSIVQ FD
!
Fig. S3 Alignment of Holcus lanatus translated isotig12721 with Oryza sativa
expansin precursor and B. distachyon expansin-B7-like protein.
3
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnets/isotig18981-095
Seaview [blocks=10 fontsize=10 LETTER] on Wed Mar 13 13:59:55 2013
AAM49810.1
LOC_Os08g45000.1
isotig18981
XP_003558116.1
isotig09507
LOC_Os10g30790.2
isotig01092
1
MGGGGGEQQQ
MGGGGGEQQQ
---------M-ARPQ---M-AREQ---M-ARQEQQQH
M-ARSE-NQG
LEVLHALNVA
LEVLHALDVA
---------LEVLSKLDAA
LEVLSALDTA
LQVLSALDAA
LQVLSALDAA
KTQWYHFTAI
KTQWYHFTAI
---------KTQWYHFTAI
KTQWYHFTAI
KTQWYHFTAI
KTQWYHFTAI
VVAGMGFFTD
VVAGMGFFTD
---------VIAGMGFFTD
VIAGMGFFTD
VVAGMGFFTD
VVAGMGFFTD
AYDLFCISLV
AYDLFCISLV
---------AYDLFCISLV
AYDLFCISLV
AYDLFCISLV
AYDLFCISLV
TKLLGRIYYR
TKLLGRIYYR
---------TKLLGRIYYH
TKLLGRVYYY
TKLLGRIYYT
TKLLGRIYYT
VDGSPSPGTL
VDGSPSPGTL
---------IDGSPTPGSL
RDGSPDPGSL
DLAKENPGSL
DLTKATPGIL
AAM49810.1
LOC_Os08g45000.1
isotig18981
XP_003558116.1
isotig09507
LOC_Os10g30790.2
isotig01092
71
PPHVSACVNG
PPHVSASVNG
---------PPNVAAAVNG
PPNVAAAVNG
PPNVAAAVNG
PPGVAAAVNG
VAFVGTLSGQ
VAFVGTLSGQ
---------VAFCGTLSGQ
VAFCGTLSGQ
VAFCGTLAGQ
VAFCGTLSGQ
LFFGWLGDKL
LFFGWLGDKL
---------LFFGWLGDKM
LFFGWLGDRM
LFFGWLGDKL
LFFGWLGDKM
GRKRVYGITL
GRKRVYGITL
---------GRKKVYGMTL
GRKRVYGMTL
GRKSVYGMTL
GRKSVYGMTL
MLMVLCSLAS
MLMVLCSLAS
---------MCMVLCSIAS
MCMVLCSLAS
LMMVICSIAS
ILMVICSIGS
ALSFGHTPTS
ALSFGHTPTS
---------GLSFGQTPTS
GLSFGSTPSS
GLSFSHTPTS
GLSFAHTPKS
VMATLCFFRF
VMATLCFFRF
---------VMATLCFFRF
VMATLCFFRF
VMATLCFFRF
VMATLCFFRF
141
AAM49810.1
WLGFGIGGDY
LOC_Os08g45000.1 WLGFGIGGDY
isotig18981
---------XP_003558116.1
WLGFGIGGDY
isotig09507
WLGFGIGGDY
LOC_Os10g30790.2 WLGFGIGGDY
isotig01092
WLGFGIGGDY
PLSATIMSEY
PLSATIMSEY
---------PLSATIMSEY
PLSATIMSEY
PLSATIMSEY
PLSATIMSEY
ANKKTRGAFI
ANKKTRGAFI
---------ANKKTRGAFI
ANKKTRGAFI
ANKKTRGAFI
ANKKTRGAFI
AAVFAMQGFG
AAVFAMQGFG
---------AAVFAMQGFG
AAVFAMQGFG
AAVFAMQGFG
AAVFAMQGFG
IITGGLVAIL
IITGGLVAIL
---------ILTGGVVTLV
ILTGGVVTLV
ILAGGIVTLI
ILAGGIVTLI
VSASFRAAFP
VSASFRAAFP
---------ISSAFRAAFD
VSAAFRAAFN
ISSAFRAGFP
ISSAFRAAYP
APPYGEDPVA
APPYGEDPVA
---------APAYKDGAMA
APAYQDGAVA
APAYQDDRAG
KPAYEDNAAG
211
AAM49810.1
STPPQADFVW
LOC_Os08g45000.1 STPPQADFVW
isotig18981
---------XP_003558116.1
STPPQADYVW
isotig09507
STPAQADYVW
LOC_Os10g30790.2 STVRQADYVW
isotig01092
SIGSEADFVW
RIILMLGALP
RIILMLGALP
---------RIILMFGAIP
RVILMFGAIP
RIILMLGAMP
RIILMLGAVP
AALTYYWRTK
AALTYYWRTK
---------ALMTYYWRMK
ALLTYYWRMK
ALLTYYWRMK
ALLTYYWRMK
MPETARYTAL
MPETARYTAL
---------MPETARYTAL
MPETARYTAL
MPETARYTAL
MPETARYTAL
VATNAKQAAA
VANNAKQAAA
---------VAKNAKQAAA
VAKNAKQAAA
VAKNAKQAAA
VAKNAKLAAA
DMSKVLQVVE
DMSKVLQVVE
---------DMSKVLQVEI
DMSRVLQVDI
DMSKVLQVEI
DMSKVLQVEL
MRNIGNN--MRNIGNN-----------GAEEDNNKAG
VATEE--K-D
QEEQD--KLE
EDETE--KME
281
AAM49810.1
---GGSRRPF
LOC_Os08g45000.1 ---GGSRRPF
isotig18981
---------XP_003558116.1
GAVEENRNSF
isotig09507
TAGDGDRNSF
LOC_Os10g30790.2 QMVTRNSSSF
isotig01092
GMVSRGNNEF
GLFSGEFVRR
GLFSGEFVRR
---------GLFSAEFLRR
GLFSNEFLRR
GLFSRQFARR
GLFSPEFARR
HGLHLVGTSA
HGLHLVGTSA
------XXST
HGLHLLGTAT
HGLHLLGTAT
HGLHLVGTAT
HGLHLVGTAT
TWLLLDIAFY
TWLLLDIAFY
TWFLLDVAFY
CWFLLDIAFY
CWFLLDIAFY
TWFLLDIAFY
TWFLLDIAFY
SQNLFQKDIF
SQNLFQKDIF
SQNLFQKDIF
SQNLFQKDIF
SQNLFQKDIF
SQNLFQKDIF
SQNLFQKDIF
SAVGWIPKAA
SAVGWIPKAA
SAVGWIPKAA
TAINWIPKAN
TAINWIPKAR
TSINWIPKAK
TAINWIHKAK
TMSALEELFR
TMSALEELFR
TMSALDELFR
TMSALEEVYR
TMSALEEVHR
TMSALEEVFR
TMSALDEVYR
351
AAM49810.1
IARAQTLIAL
LOC_Os08g45000.1 IARAQTLIAL
isotig18981
IARAQTLIAL
XP_003558116.1
IARAQTLIAL
isotig09507
IARAQTLIAL
LOC_Os10g30790.2 IARAQTLIAL
isotig01092
ISRAQTLLAL
CGTVPGYWFT
CGTVPGYWFT
CGTVPGYWFT
CGTVPGYWFT
CGTVPGYWFT
CGTVPGYWFT
CGTVPGYWFT
VTLIEVVGRF
VALIDVVGRF
VALIDVVGRF
VALIDRIGRF
VALIDRIGRF
VFLIDIVGRF
VFLIDVIGRF
KIQAVGFFMKIQAVGFFMAIQVMGFFFWIQLGGFFFWIQLGGFFFAIQLLGFFMAIQLHGILHD
--MTLFMLTL
--MTLFMLTL
--MTVFMLGL
--MTVFMLCL
--MAVFMLGL
--MTVFMLGL
DRLHARSRRT
ALPYHHWTAP
ALPYHHWTAP
AVPYHHWTAP
AAPYHHWTTP
AFPYHHWTTP
AVPYHHWTTK
IPPLDDARQP
GKNHVGFLLL
GKNHVGFLLL
G-NHVGFVVL
G-NHIGFVVL
G-NHIGFVVL
G-NHIGFVVM
SR-LRGHVRL
421
AAM49810.1
YGLTFFFANF
LOC_Os08g45000.1 YGLTFFFANF
isotig18981
YGLTFFFANF
XP_003558116.1
YGLTFFFANF
isotig09507
YGLTFFFANF
LOC_Os10g30790.2 YAFTFFFANF
isotig01092
----------
GPNSTTFIVP
GPNSTTFIVP
GPNSTTFIVP
GPNSTTFIVP
GPNSTTFIVP
GPNSTTFIVP
----------
AEIFPARLRA
AEIFPARLRA
AEIFPARLRS
AEIFPARLRS
AEIFPARLRS
AEIFPARLRS
-DLLLRQLRS
TCHGISAASG
TCHGISAASG
TCHGISAASG
TCHGISAAAG
TCHGISAASG
TCHGISAAAG
QRNHIRRAGG
KLGAIVGSFG
KLGAIVGSFG
KLGAIAGGFG
KLGAIIGSFG
KLGAIVGSFG
KAGAIIGSFG
DLP------G
FLYLAQSPDR
FLYLAQSPDR
FLYLAQSPDP
FLYLAQSQDP
FLYLAQNQDQ
FLYAAQ--DP
KAAVDVPRDI
SKTEHGYPPG
SKTEHGYPPG
AKTDHGYAAG
AKVDHGYKAG
KLVDHGYKAG
HKPDAGYKPG
SRLREGWSHD
491
AAM49810.1
IGVRNSLFLL
LOC_Os08g45000.1 IGVRNSLFLL
isotig18981
IGVRNSLFIL
XP_003558116.1
IGVRNSLFIL
isotig09507
IGVRNSLFIL
LOC_Os10g30790.2 IGVRNSLFVL
isotig01092
RGIRVPLRRT
AACNLLGLLF
AACNLLGLLF
AGCNLLGMLL
SVCNFLGMGF
AACNFLGMVF
AGCNLLGFIC
G---------
TFLVPESKGK
TFLVPESKGK
TFLVPESKGK
TFLAPESNGL
TFLAPESNGI
TFLVPESKGK
----PAQAGR
SLEEMSGDAE
SLEEMSGDAE
SLEEMSGENE
SLEELSGENE
SLEELSGEND
SLEEMSGEAE
WVQ---GRDR
AQEEAPPPLAQEEAPPPLV-IGAEATGDGEDQPAPAE-ETTAAPADDDDEVAAAG
NP--------
------QTV------QTV---YNR-TVP
---HAR-TVP
---HAR-TVP
GGAAVRPQT----------
L
L
V
V
V
A
-
Fig. S4 Alignment of Holcus lanatus translated isotigs 01092, 18981, 09507 with
Oryza sativa putative high affinity phosphate transporter HAPT (AAM49810.1), O.
sativa putative phosphate transporters (LOC_Os08g45000.1, LOC_Os10g30790.2)
and Brachypodium distachyon phosphate transporter 1-4-like isoform 2
(XP_003558116.1).
4
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnet
Seaview [blocks=10 fontsize=10 LETTER] on Wed Mar 13 13:50:29 2013
LOC_Os06g29790.1
Q651J5
isotig16690
1
MATIETHYHC YSLHQSTKRK VEEEDLQGER RPMVKFSKQF EGQLVPEWKD AFVDYWQLKK DIKRLQAAEA
---------- ---------- ---------- --MVKFSKQF EGQLVPEWKD AFVDYWQLKK DIKRLQAAEA
---------- ---------- ---------- ---------- ---------- ---------- ----------
LOC_Os06g29790.1
Q651J5
isotig16690
71
EAAGVAATTP LSQCQAPVAA AHWVMRLPFL HPHGHHHKEH GAIQVHRKLA SGGGGGGGAV AGEVYETELV
EAAGVAATTP LSQCQAPVAA AHWVMRLPFL HPHGHHHKEH GAIQVHRKLA SGGGGGGGAV AGEVYETELV
---------- ---------- ---------- ---------- ---------- ---------- ----------
141
LOC_Os06g29790.1 DGGAGFADGE AARAFFARLD EQLNKVNRFY ERKEAEFVER GESLRRQLQI LAELRAAVVA EQQRDGRRRR
Q651J5
DGGAGFADGE AARAFFARLD EQLNKVNRFY ERKEAEFVER GESLRRQLQI LAELRAAVVA EQQRDGRRRR
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------211
LOC_Os06g29790.1 CGNGGDSSPP DTEDPSVSCS ILHGDQSLRG TSEQEQEGQE KLTKDMIARS PDEGDDDQLT IPQELGDSGR
Q651J5
CGNGGDSSPP DTEDPSVSCS ILHGDQSLRG TSEQEQEGQE KLTKDMIARS PDEGDDDQLT IPQELGDSGR
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------281
LOC_Os06g29790.1 LGRPREEAAN TRPRTTLPGG RAVTCQGRSV RINIPVTTPT RTVTAIRELL FDDMLSQSRR SGSANGTKCG
Q651J5
LGRPREEAAN TRPRTTLPGG RAVTCQGRSV RINIPVTTPT RTVTAIRELL FDDMLSQSRR SGSANGTKCG
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------351
LOC_Os06g29790.1 DKLSINKRKV HQAEKMIRGA LIELYKGLGY LKTYRSLNMM AFVKILKKFD KVTAKEAQSI YLKVVESSYF
Q651J5
DKLSINKRKV HQAEKMIRGA LIELYKGLGY LKTYRSLNMM AFVKILKKFD KVTAKEAQSI YLKVVESSYF
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------421
LOC_Os06g29790.1 NVSDKVIRLM DDVDELFVRH FAEGDKRKAM KYLKPNQREE SHTTTFFIGL FTGGFAALFI GYCIMAHIAG
Q651J5
NVSDKVIRLM DDVDELFVRH FAEGDKRKAM KYLKPNQREE SHTTTFFIGL FTGGFAALFI GYCIMAHIAG
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------491
LOC_Os06g29790.1 MYTQQSNKVY MATSYPVLSM FSLFFLHLFL YGCNIFMWRK TRINYTFIFE FTPTKELKYR DVFLICTTSM
Q651J5
MYTQQSNKVY MATSYPVLSM FSLFFLHLFL YGCNIFMWRK TRINYTFIFE FTPTKELKYR DVFLICTTSM
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------561
LOC_Os06g29790.1 TIVIGVMFAH LTLIVKGYSS CAVQAIPGAL LLVFLLILVC PFNILYRSCR YHFLTVIRNI ILTPFYKVVM
Q651J5
TIVIGVMFAH LTLIVKGYSS CAVQAIPGAL LLVFLLILVC PFNILYRSCR YHFLTVIRNI ILTPFYKVVM
isotig16690
---------- ---------- ---------- ---------- ---------- ---------- ---------631
LOC_Os06g29790.1 VDFFMADQLC SQVPLLRSLE YLACYYITSS YKTQDYGYCT RVKHFRDLAY AVSFLPYYWR AMQCARRWFD
Q651J5
VDFFMADQLC SQVPLLRSLE YLACYYITSS YKTQDYGYCT RVKHFRDLAY AVSFLPYYWR AMQCARRWFD
isotig16690
---------- -------SLE YLACYYITSS YKTQDYGYCT RVKHFRDLAY AVSFLPYYWR AMQCARRWFD
701
LOC_Os06g29790.1 EGDINHIVNL GKYVSAMLAA GTKVAYENDN SAGWLSLVVI VSSLATIYQL YWDFVKDWGL LQFNSKNPWL
Q651J5
EGDINHIVNL GKYVSAMLAA GTKVAYENDN SAGWLSLVVI VSSLATIYQL YWDFVKDWGL LQFNSKNPWL
isotig16690
EGDINHIVNL GKYVSAMLAA GTKVAYENDN SAGWLSLVVI VSSIATIYQL YWDFVKDWGL LQFNSKNPWL
771
LOC_Os06g29790.1 RNDLILKQKY IYFLSMGLNL ILRLAWLQTV IHPNIGSLDS RVTLFILAAL EVIRRGHWNF YRLENEHLNN
Q651J5
RNDLILKQKY IYFLSMGLNL ILRLAWLQTV IHPNIGSLDS RVTLFILAAL EVIRRGHWNF YRLENEHLNN
isotig16690
RNDLILKQKY IYFISMGLNL LLRLAWLQTV IHPNIGSLDS RVTLFFLAAL EVIRRGHWNF YRLENEHLNN
841
LOC_Os06g29790.1 AGKFRAVKVV PLPFHEVEED
Q651J5
AGKFRAVKVV PLPFHEVEED
isotig16690
AGKFRAVKVV PLPFHEVEED
Fig. S5 Alignment of Holcus lanatus translated isotigs 16690 with an Oryza sativa
phosphate transporter Phosphate transporter PHO1-3 (Q651J5) and O. sativa
putative phosphate transporters 1 (LOC_Os06g29790.1)
5
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnet
Seaview [blocks=10 fontsize=10 LETTER] on Mon Mar 18 20:31:13 2013
isotig11028
XP_003560938.1
LOC_Os06g10750.1
1
MG-VGKKVLG DLKPYLMMVL LQVGYAGMYI VSVASLKQGM SHFVLVTYRN VAAAAVMTPF AIIFERGLRP
MGFLGKRVVE DVKPYVMMVF LQFGYAGMYI VSVASMKRGM SHFVLVTYRN LVATILMTPF ALYFERGARP
MG-VG-RALS DAKPYVAMVL LQVGFAGMYI VSVASLKRGM NHFVLVVYRN LVATVLMAPF ALLLERGVRP
isotig11028
XP_003560938.1
LOC_Os06g10750.1
71
KVTMPIFIKI CGLALLEPVI DQNLYYLGNK LTSASFSSAL VNILPAVTFI MAIILRMEKL RLRSLHSQAK
KMTVPIFIKV CGLAFLEPVL DQNLYYLGTK LTSASFSSAL VNILPAVTFL MAIILRMEKL RLRSRHSQAK
KMTLRIFLKI MGLAILEPVL DQNLYYMGAK LTSAGFASAL VNILPAVTFL LAVLLRMEKV RLRSLHSQAK
141
isotig11028
VAGTICTVTG AVLMIMYHGP VVQFPWA-RG A----HHADP S--AAAAAQS SATWLTGTIM IIGSCVAWAG
XP_003560938.1
VMGTICTVTG AVLMIMYHGP VVRFPWSPRG L----HHGDA SAAAAAAAQS SGTWLKGTIT VIGSCVAWAG
LOC_Os06g10750.1 IAGTVFTVAG AVLMIMYHGP VVQFPWSSSA SGSAGHHVDG AAAAAATASS ASSWLNGTVM LVGSCVCWSG
211
isotig11028
FFVLQSNTLN SYPAPLTLTS VICAMGAVIN GAVTLVAERR DMGAWAVGFD TRLFTVVYSG VVCSGVAFYV
XP_003560938.1
FFVLQSNTLR SYPAQLSLTS LICVVGTTMS GSVALVAERR DMSAWKIGFD TRLFTVIYSG VVCSGVAYYV
LOC_Os06g10750.1 FFILQNNTLQ SYPAELSLTA LICVLGSAMS GAVALVAERR DMSVWVIGFD TRLFTAVYSG IVCSGVAYYV
281
isotig11028
QGLVTRTRGP VFVTAFQPLC MIITAVMGSI LLKEETTLGS VIGAAIIVLG LYSLIWGKSN DI-LD-GKPXP_003560938.1
QGLVTKTRGP VFVTAFQPLC MIITAVLGST LLKEETTRGS VIGAAIIVVG LYSLIWGKSN DVDLDNEKPLOC_Os06g10750.1 QGLVTRARGP VFVTAFQPLC MIITAVLGST ILKEEITLGS VIGAVIIVVG LYALIWGKGG DH-ADNGKPP
351
isotig11028
-ANSVA-EKL ALPLTSVGAN GHSCNGGANG GANGGRHVCG GQVADDVETP A---KGAIFV
XP_003560938.1
----AAQ-KL ALPLTTV-AN GNGANHGAAG --NGSRHVCG GQVAHDAETP APALKGVCYLOC_Os06g10750.1 AAAAAAPEK- GLPLTTLQAN GDG------- --DG-KLAV- --LVADVEMP A--VKDV-Y!
Fig. S6 Alignment of Holcus lanatus translated isotig 11028 with Oryza sativa integral
membrane protein DUF6 containing protein, expressed and Brachypodium
distachyon predicted auxin-induced protein 5NG4-like.
6
Fig. S7 Alignment of Holcus lanatus translated isotigs 19077 against a H. lanatus
CDC25-like tyrosine phosphatase reported by Bleeker et al. (2006).
7
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmne
Seaview [blocks=10 fontsize=10 LETTER] on Wed Mar 20 11:50:40 2013
isotig02053
XP_003557359.1
NP_001189502.1
1
MEIAAAPVS- PAPRTVEDIY KDFSNRRAAL VRALTVDVDE FFGYCDPEKE NLCLYGYSNG RWEVALPAEE
MEMAPAPVS- PTPRTVDDIY KDFSNRRTAL VRALTVDVDD FYGFCDPEKE NLCLYGYPNG SWEVALPAEE
ME----GITH PIPRTVEEVF SDFRGRRAGL IKALTNDMVK FYQTCDPEKE NLCLYGLPNE TWEVNLPVEE
isotig02053
XP_003557359.1
NP_001189502.1
71
VPPEMPEPAL GINFARDGMH RRDWLSLVAV HSDSWLLSIA FFFGAR--LN ANERKRLFSM INDQPTVLES
VPPEMPEPAL GINFARDGMN RRDWLSLVAV HADSWLLAVA FFFGAR--LN ANDRKRLFSM INDQSTVLES
VPPELPEPAL GINFARDGMQ EKDWVSLVAV HSDSWLLSVA FYFGARFGFG KNERKRLFQM INELPTIFEV
141
isotig02053
LS-ERKHGRD NKSGVENSSK SRHS-AK--R ANNDMQTKNS RPGVVDDAYE DDDEHSETLC GTCGGRYNAS
XP_003557359.1 LS-ERKHGRD NKSGGDNSGK SRHS-AK--R ANNDVQTKNP RPVAVDVAYE DDDEHSETLC GTCSGRYNAS
NP_001189502.1 VSGNAKQSKD -LSVNNNNSK SKPSGVKVAK MSS-PPPKEE EEEEDESEDE SEDDEQGAVC GACGDNYGTD
211
isotig02053
EFWIGCDICE RWFHGKCVRI TPAKAEHIKH YKCPDCSSKK GRQ
XP_003557359.1 EFWIGCDICE RWFHGKCVRI TPAKAEHIKH YKCPDCSSKK SRQ
NP_001189502.1 EFWICCDACE KWFHGKCVKI TPAKAEHIKH YKCPTCSNKR ARP
Fig. S8 Alignment of Holcus lanatus translated isotig 02053 with Arabidopsis thaliana
ALFIN-LIKE 6 protein and Brachypodium distachyon PHD finger protein ALFIN-LIKE 2like.
8
Alignment: /Users/carolinemeharg/Desktop/RecentSubmittedPapers/Andy-holcus/AmCm-Hocus-ForPublication/isotig-alignmnets/
Seaview [blocks=10 fontsize=10 LETTER] on Fri Mar 15 11:48:11 2013
isotig09604
LOC_Os03g01700.1
1
MV-LASLPKV VLGSLAFAVF WMMAVFPSVP FLPIGRTAGS LLSAVLMIVF HVISPDDAYA SIDLPILGLL
MSELASAPKV ALGSIAFAVF WMMAVFPSVP FLPIGRTAGS LLSAVLMVIF HVISPDDAYA SIDLPILGLL
isotig09604
LOC_Os03g01700.1
71
FSTMVVGGYL KNAGMFKHLG TLLAWKSQGG RDLLCRVCVV TALASALFTN DTCCVVLTEF VLELAAERNL
FATMVVGSYL RNAGMFKHLG RLLAWKSQGG RDLMCRVCVV TALASALFTN DTCCVVLTEF VLELAAERNL
141
isotig09604
PAKPFLLALA SSANIGSSAT PIGNPQNLVI AFNSKISFPK FLLGILPAML AGMAVNMVML LCMYWKDLEG
LOC_Os03g01700.1 PAKPFLLALA SSANIGSAAT PIGNPQNLVI AFNSKITFPK FLMGILPAML VGMAVNMVML LCMYWRELGG
211
isotig09604
ASPDMVDSGK QMTAVEEGRS PSIASLKSPH PLNGSTVNDD GESM--ME-- ENLSTKHPWF MQCTEERRKL
LOC_Os03g01700.1 GAELSVD-GK QMEAVEEGRS P--ASAKSTP QLNG-----N GNTMMSLEMS ENITTKHPWF MQCTEARRKL
281
isotig09604
FLKSFAYIVT VGMVVAYMVG LNMSWTAITT AIALVVVDFR DAEPCLVKVS YSLLVFFSGM FITVSGFNKT
LOC_Os03g01700.1 FLKSFAYVVT VGMVVAYMVG LNMSWTAITT ALALVVVDFR DAEPCLDTVS YSLLVFFSGM FITVSGFNKT
351
isotig09604
GLPSAIWNFM APYSKVNSVG GISVLSVIIL LLSNLASNVP TVLLMGNEVA TAAALISPAA VTRSWLLLAW
LOC_Os03g01700.1 GLPGAIWDFM APYSKVNSVG GISVLSVIIL LLSNLASNVP TVLLMGDEVA KAAALISPAA VTTSWLLLAW
421
isotig09604
VSTVAGNLSL LGSAANLIVC EQARRAPRNA YELTFWNHLI FGVPSTLIVT AVGIPLIGKM
LOC_Os03g01700.1 VSTVAGNLSL LGSAANLIVC EQARRAPRNA YDLTFWQHIV FGVPSTLIVT AVGIPLIGKI
Fig. S9 Alignment of Holcus lanatus isotig09604 with LOC_Os03g01700.1 an
expressed Oryza sativa protein (arsB-like, putative arsenite transport protein /cation
related transport protein).
9