The microbes associated with our food tend to have a bad name – food poisoning is often in the news. Yet while some make us ill and others can be a nuisance by spoiling our food, without microbes there would no bread, cheese, beer or chocolate. Friend or foe – food microbes are always on the menu. MICROBES & FOOD PRODUCTION For thousands of years, humans have taken advantage of natural fermentations to make a diverse range of products, including bread, yoghurt, cheese and alcoholic drinks, although until relatively recently no one understood that micro-organisms were responsible for these processes. Fermentation improves the taste, texture and smell of food products through the conversion of sugars into acids. It also causes changes that reduce the growth of unwanted food microbes. This improves the food’s storage life and safety. Nowadays fermentations are used to make an amazingly wide range of food and drink, including salami, soy sauce, olives and chocolate. Microbes are involved in the production of foods in other ways – they are cultured to provide food additives such as enzymes, vitamins and organic acids that provide flavour, texture or nutritional value to foods or help preserve and stabilize them. Examples include citric acid for preserving and flavouring soft drinks, and dextran and xanthan for stabilizing salad dressings, ice cream and gluten-free bakery products. Some foods are made directly from microbes; mycoprotein, such as Quorn®, is made from the mycelium (tangled network of threads) of cultured mould, and Marmite® is made from the yeast left over from brewing. Edible mushrooms, the fruiting bodies of certain filamentous fungi, are a common item in our diet. They include many fungal species that are either harvested wild or cultivated. Agaricus bisporus is the most commonly grown mushroom in the UK and accounts for 95% of the total British market. Several sizes are sold; button, closed cup, open cup and large open mushrooms or flats. These correspond to different phases in the growth cycle of the mushroom and are not different varieties or species. Exotic types like shiitake, oyster mushrooms and chanterelles are now joining this familiar mushroom on the supermarket shelves. 2 3 Lactic acid fermentations Lactic acid fermentation is predominantly caused by lactic acid bacteria. Important lactic acid bacteria include Lactobacillus, Leuconostoc, Pediococcus, Streptococcus and Lactococcus. Lactic acid bacteria convert sugars to lactic acid. The lactic acid gives the food its characteristic sour taste. The production of lactic acid causes a decrease in pH which inhibits the growth and survival of other microbes. This contributes to the improved microbiological safety of the food. FERMENTED FOODS Foods made by lactic acid fermentations include: Fermentation is a process by which Dairy products bacteria – usually lactic acid bacteria – Lactic acid bacteria are used in the fermentation of milk to yeasts and moulds convert sugars produce many dairy products, for example cheese, yoghurt and and carbohydrates (complex organic fermented milk drinks such as kefir. Kefir is a popular drink in compounds) into less complex pro- Russia, a product of a mixed lactic acid bacteria and yeast ducts such as carbon dioxide, alcohol fermentation. and organic acids. Microbes break down complex molecules in food Fermented meats to provide the energy they require for growth. As by-products they Salami, pepperoni, chorizo and nham (an uncooked, fermented, sometimes produce the fermented semi-dry Thai sausage popular in Asia) rely on a combination of foods and drinks that we enjoy. lactic acid fermentation with curing salts and drying. Yeasts and moulds may also contribute to the flavour of these products. For many years fermentation was a hit and miss process which relied on Fermented vegetables naturally occurring microbes getting into our food. For example, yeasts Sauerkraut, olives, gherkins and cucumber pickle are made by lactic in the air would land in fruit juice fermentation of the brine-soaked food. Successions of different and ferment the natural sugars in it, lactic acid bacteria are involved, sometimes together with certain producing wine. yeasts and moulds. Microbes and fermentation are now better understood and each process is Other foods made by lactic acid fermentations include sour carefully controlled from start to finish. dough bread, miso, dosa (fermented pancakes) and surströmming Special cultures of microbes are used in (fermented herring). Some of the flavours in wine are partly due the fermentations to give the product to fermentations by bacteria, which break down malic acid in the the required properties. fruit juice to lactic acid and carbon dioxide gas. 4 5 CHEESE Ripening Most types of cheese have to be ripened during which water is lost Cheese has been produced for over 7,000 years and microbes are crucial for its and a series of biochemical changes manufacture. In ancient times, milk used to be stored in bags made from the causes the flavour and texture of the stretchy lining of cows’ digestive systems. Eventually the milk would go sour cheese to develop. The type of cheese and form solid lumps that could be squashed together to make cheese. This produced is partly dependent on the was due to the presence of a digestive enzyme called chymosin (rennin) that types of microbes used during this is secreted by ruminants’ guts, and bacteria that fermented the lactose sugar process. Ripening takes from a few in the milk into lactic acid. days to years. How is cheese made? Soft cheeses Milk contains the sugar lactose and the protein casein as well as fat, water, Brie and Camembert are sprayed with minerals and vitamins. During the cheese-making process, the lactose is the fungus Penicillium camembertii converted to lactic acid and the protein is coagulated. The water is removed, that forms a soft white mycelium leaving a solid or semi-solid product. It is important to use high-quality milk, on the surface, giving the cheese its which has been pasteurized to kill any pathogenic (harmful) bacteria. characteristic flavour and texture. Fermentation Blue cheeses j A mixed starter culture of lactic acid bacteria (such as types of Lacto bacillus, Lactococcus and Streptococcus) is added to the milk to start These are inoculated with cultures of the fermentation. different moulds and yeasts. Some- j The fermentation runs for about an hour during which time the pH falls, times the microbes are added to milk or curds in the vat; others are due to conversion of the lactose to lactic acid by the bacteria. inoculated into the formed cheeses using wires. Penicillium roquefortii is Curdling used to produce Gorgonzola, Stilton j Chymosin is added to ripe milk to produce a solid product called curds. and Dolcelatte. The spores quickly j The curds are separated from the liquid part (called the whey). germinate into fungal hyphae (thread- j The curds are washed, salted, chopped and pressed into moulds to make like structures that form the mycelium), solid cheese. which run along cracks in the cheese. Hard cheeses Cheeses like Cheddar contain less than 40% water. During manufacture, the cut curds are heated to 38–42 °C, which makes them shrink and become firmer. The starter culture can survive the heat and continues to grow and produce acid. When the acid level has reached around 0.25%, the whey is run off and the curds are formed into blocks in a manual process called cheddaring. The blocks are pressed to remove any remaining whey and trapped air and the cheese is then left to mature. 6 7 YEAST FERMENTATIONS Yeasts are single-celled fungi that are very common in the environment, but are usually isolated from sugar-rich material such as the skins of fruits and berries. Yeasts are used in the fermentation of fruits to produce wines, cereals to make beer, in bread-making and flavouring in the form of yeast extract. Bread A yeast called Saccharomyces cerevisiae is used in bread-making. It is more commonly known as baker’s yeast. It is thought to have originally been isolated from the skins of grapes. (The yeast forms a thin white film which can be seen on the skins of fruit such as grapes and plums.) Bread is made by mixing yeast with sugar, flour and warm water. The yeast uses the sugar and the sugars present in the flour as its food. It breaks them down to provide energy for growth. The yeast grows by budding; as it does, bubbles of FASCINATING FACT the gas carbon dioxide are produced in the dough. The bubbles make the dough expand and rise. This is because the dough is extremely sticky and it traps the bubbles, preventing them from escaping. When What is noble rot? the dough is baked, the heat kills the yeast and the dough stops expanding. Botrytis cinerea is a grey mould that is commonly found growing on Baker’s yeast comes in two forms: fruit and vegetables. It is a problem, particularly on strawberries, where it j compressed fresh yeast – the yeast is alive but because it contains 70% moisture it does not keep well and must be refrigerated and used quickly after opening. causes spoilage of the fruit. However, under the right conditions (damp, misty mornings which promote the growth of the mould, followed by j dried into granules – the yeast is alive but dormant (inactive) due to lack of moisture. As it only contains 8% water, dried yeast has a longer shelf life than fresh yeast and does not need to be refrigerated. warm, dry sunny afternoons) the presence of the mould on grapes can be advantageous to wine growers. Botrytis spores draw moisture from Alcoholic drinks the grapes causing the fruit to break Beer and wine are alcoholic drinks made from fermented barley and grapes, This concentrates the sugars, mag- respectively, but alcoholic drinks can be made from a wide range of plant nifying the flavours, to produce a material containing fermentable carbohydrates, for example Japanese sake is very intense, sweet wine. Under made from rice. Spirits such as vodka and whisky have higher alcohol con- these circumstances the fungus has tents and are made by distilling the alcohol produced in fermentation. The been referred to as La Pourriture organisms that carry out the fermentation are Saccharomyces yeasts. Noble – the noble rot! down and become partially raisined. 8 9 OTHER FERMENTED AND FUNGAL FOODS Chocolate Cocoa beans are seeds which form inside pods on the cacao tree (Theobroma cacao). When they are harvested, they are coated in a slimy layer of sugary material. Microbes are used to break down this material and prepare the beans for roasting. The fermentation of cocoa beans is complex and involves a succession of microbes, starting with yeasts, continuing with bacteria and finishing with moulds. The alcohols, acids and heat formed during fermentation induce complex biochemical reactions inside the beans. Unfermented beans do not produce the chocolate flavour and scientists have been unable to replicate in the laboratory the complicated biochemistry that takes place naturally on the farm. Chocolate is a food, which can only be made with the help of microbes! A similar, but less complex method is used for the processing of coffee beans to make coffee. FASCINATING FACT What makes the centre of After Eight® mints gooey? j The contents of the cocoa pods are scooped out Invertase is an enzyme derived from yeast. It breaks down the j The beans are turned daily to aerate them. easily crystallized sugar sucrose and piled in heaps. j Microbes occurring naturally on the plant cause a fermentation which lasts for 7 days. j When fermentation has finished, the beans are sugars glucose and fructose. This dried and taken to the processing plant where glucose/fructose mixture is often they are roasted. syrup. The fondant in the centre of After Eight® mints is made as a stiff paste of sucrose, water and a small amount of the enzyme 11 into the less easily crystallized referred to as invert sugar or 10 Making chocolate j They are then kibbled (broken into pieces); the broken shells are removed and the small pieces left (called nibs) are reduced to a thick liquid, which contains about 55% cocoa butter. invertase. The invertase turns the j Half the cocoa butter is removed by pressing hard sucrose into the soft centre. and used to make chocolate bars. The remain- This process may take over 3 ing cocoa solids are ground up to make cocoa months. powder. FASCINATING FACT What links microbes to MSG? Monosodium glutamate (MSG) is a food ingredient used to enhance flavour. The bacterium Corynebacterium glutamicum produces the amino acid glutamic acid from the fermentation of molasses. Mycoprotein Glutamic acid is then converted into the salt monosodium glutamate. Technically, humans can detect only four different tastes: salty, sweet, sour and bitter (all other complex flavours are a combination of Mycoprotein has been on sale to the public as Quorn® since 1985 taste and smell). But there is now thought to be a fifth: the ability to detect MSG. This is described as a and is a popular meat substitute, particularly with vegetarians. It meaty sort of taste that the Japanese call umami. MSG has been extensively researched and approved for is the filamentous mycelium of Fusarium venenatum, which was use in food, but has attracted controversial publicity due to fears by some consumers that it may cause originally isolated from soil. headaches and nausea and trigger asthma attacks. Most of this evidence is anecdotal and scientific Manufacture of Quorn® research has not been able to substantiate these adverse affects. The mould is grown in a nutrient-rich medium in a bioreactor called a chemostat. This is a continuous culture system that gives good productivity. The fermenter runs for 6 weeks and is then cleaned for 2 weeks and prepared for the next run. The biomass and culture medium are separated and the mycoprotein is heated to 64 °C for 20–30 minutes to break down the genetic material (RNA) of the mycelium which is too high for human consumption. The final product is collected by filtration under vacuum. A mechanical process is used to align the mycelial filaments and the resulting product is frozen for storage, flavouring and distribution as Quorn®. Truffles Truffles are the fruiting bodies of filamentous fungi. The fruiting body is small, potato-like, FASCINATING FACT warty and gnarled in appearance, and can be brown, white or black. Some types weigh over 1 kg. There are many species of truffle, but only some are eaten. Truffles have a distinct flavour and strong smell. How do we flavour soft drinks? The fungus grows as a tangled network of threads called hyphae in association with the roots of certain trees. This is a symbiotic relationship where each organism benefits from the other. The truffles get their sugars for growth from the root cells and in return pass nutrients from Citric acid is an organic acid found the soil to the roots. Truffles are the fruit of the fungus and contain spores. in citrus fruit. It is used as a sour flavouring and is produced by fer- Truffles rely on being eaten to spread their spores. Animals are attracted by the scent and menting molasses or other sugar dig under the leaf litter to find the fruiting bodies. After eating the truffle, the spores pass substrates with the filamentous through the animal’s body and are distributed into the environment with their faeces. Truffle- fungus hunters use animals to hunt out the valuable fungi, which can be sold for a very high price. Aspergillus niger. This fungus is also used to produce Traditionally pigs were used but now highly trained dogs seek out the truffles. another food ingredient called Truffles are so expensive and have such an intense flavour that they are used in very tiny gluconic acid (E574) that is used amounts to flavour food. Some are used to make truffle oil for sprinkling on gourmet dishes. as an acidity regulator and is naturally found in tea, fruit and A white truffle weighing 1.5 kg sold for £165,000 in 2007. It was unearthed in woods near wine. Pisa in Italy and was one of the largest truffles ever found. 12 13 SGM MEMBERS’ RECIPES BAGELS – Method bread with a difference 1. Put the milk, butter and sugar into a mixing bowl and whisk thoroughly. The milk should be hot enough to melt the butter completely. 2. When the mixture is lukewarm, sprinkle on the yeast and leave to rise. This will take some time, typically more than half an hour. To save time the yeast can be started off in warm water and the amount of milk reduced to allow for the water. 3. Stir in the egg white and salt, then beat in the flour to make a soft Legend has it that the dough. first bagel was made by a Viennese baker in 4. Turn the dough onto a floured board and kneed until smooth and elastic. 1683 who wanted to pay tribute to the King of Poland, Jan Sobieski, for saving the Austrian people from 5. Allow it to rise until doubled in size (about 1 hour). Do not allow the surface Dave Roberts’ to dry out and form a skin as this restricts the rise. To prevent this, use either Basic Bagel Recipe a large plastic bowl with a snap-on lid or cover the mixing bowl with cling film or a wet tea towel. Turkish invaders. Knowing the 6. Knock the dough back and knead briefly to remove the largest gas king was a distinguished horseman, the bubbles. baker shaped the dough into 7. Divide the dough into 16 balls about the size of a small orange. Push a Ingredients floured finger through the centre of one of the balls. Twirl the bagel around a stirrup. The Austrian word for stirrup is bagel. The popularity of the bagel spread throughout Eastern Europe, evolving as it did so into its characteristic doughnut 225 ml milk, scalded the finger on the work surface (think hula-hoop) until the hole is at least one (i.e. boiled & hot) third the diameter of the whole. Repeat for the other balls. 8. Place them on a tray and leave in a damp place. Dave uses a cake cooling 50 g butter rack to support a wet tea towel over the dough balls. 25 g castor sugar 9. Leave them to rise until they look puffy, which can take as little as 10 or 15 minutes. Don’t wait too long – they should not double in size. 2 tsps dried yeast shape. During the 1880s, thousands of Eastern 10.Preheat the oven to 200 °C (Gas mark 6). 1/2 tsp salt European Jews emigrated to America taking with them this unique type of bread. Since then 11. Prepare a large shallow pan of gently boiling water. White of 1 medium egg 12.Put the bagels into the water a few at a time so that they don’t touch one 400 g strong plain flour another. They will float about halfway in and out of the water. Leave them to rise for 15 or 20 seconds then lift them out with a slotted spoon, and the bagel has grown in arrange them onto a greased baking tray. They won’t rise much more than popularity and is now the size they are now. a standard food item on supermarket shelves 13. Bake for about 20 minutes, until they are golden brown and sound hollow across the UK. What makes the bagel when you tap them. so 14. Transfer to a baking rack to cool. different from ordinary bread is the cooking process. It is the only Extras dough that is boiled before it is baked. It is Toppings like seeds can be added to the surface of the bagel prior to baking. The this procedure that gives dough can be sweetened by adding sugar to the flour. Different types of bagel the bagel its unique can be made by the addition of, for example, raisins to the basic dough after texture and glossy crust. kneading has taken place. 16 17 Dave Roberts’ elderflower ‘champagne’ Method Ingredients 1. Shake flower heads to remove any insects. The flower heads 2 large heads of fully open elderflowers can be washed before use in cold water and then patted dry on kitchen paper. Care must be taken not to bruise the flower 30 ml white wine vinegar heads, as they are very delicate. 1 large lemon 2. Pick the florets from their stalks and put them into a suitable 680 g sugar container, such as a plastic bucket with a loose-fitting lid. It should have a capacity of at least 7 litres. 4.5 litres cold water 3. Thinly pare the lemon (no white pith) and cut up the parings This recipe makes 5 litres into fine particles. 4. Juice the lemon and add the parings and the juice to the bucket. 5. Add the cold water and the vinegar, then stir in the sugar until it has dissolved. ELDERFLOWER ‘CHAMPAGNE’ – 6. Cover and leave in a warm place for 24 hours. 7. Strain the liquid and pour into suitable bottles, leaving a 50 mm gap. an elegant, sparkling fermented drink NB: Suitable bottles are screw-topped 1 litre bottles that previously held carbonated drinks, like tonic water, etc. Avoid bottles with shoulders (like red wine bottles). If you use glass bottles, they must be strong enough to withstand the The fragrant flower heads from pressure that will build up during fermentation. the elder tree can be used to make a refreshing summer drink called 8. Put the caps on tight and lay the bottles on their sides elderflower ’champagne’. The elder is somewhere where the temperature will stay reasonably a common native tree found grow- constant at around 21 °C. After 2 weeks, move them to a ing in mixed deciduous woodland, cool store. Serve cool, but not cold. Drink the elderflower marshy woodland, thickets, clearings, ‘champagne’ within 4 weeks. hedgerows and gardens. It flowers in late spring, producing an off-white NB: The bottles are laid on their side to minimize damage flower, which grows to approximately from flying bottle tops should the brew explode. Take care 5 to 6 mm in size. The best time to when opening the bottles. You can take the top off under a pick the flowers is on a dry warm wet tea towel and you should at least open them over the day when they are in full bloom. It is sink. They have a tendency to be volcanic when first opened. important that the flowers are not collected from busy roadsides where Microbiology they may be exposed to air pollution. The addition of yeast is not required The fermentation of elderflower ‘champagne’ relies on the natural as wild yeasts that occur naturally on flora of the plant material, but Dave tells us that the yeasts in the the flowers ferment the elderflower sediment at the end of the process are not the same as the ones ‘champagne’. that can be isolated from the flowers at the start. 18 19 GINGER BEER – a very refreshing, old-fashioned, fermented drink Ginger beer originated in England, in the mid-1700s, where it became the favourite drink for over 150 years. The forerunners of ginger beer were mead and metheglin (mead flavoured with herbs or spices). Mead is a yeast-fermented honey and water drink and has been around for a very long time. Records show that the Celts were drinking it as long ago as 500 AD. It is reputed to be one of the oldest recorded fermented beverages. Ginger beer also uses home-baked bread. In yeast for fermentation and is sweetened with honey, molasses or England cane sugar. The yeast converts the sugar into ethanol (alcohol) the popularity of ginger and carbon dioxide (gas). Other ingredients include fresh whole beer peaked in 1935. The ginger, although in Libby’s recipe this has been replaced with USA had 300 breweries, ground ginger and lemons or lemon juice. The yeast is also said Canada had over 1,000 to add a special flavour to the beer, which is similar to that of and England had 3,000! Ingredients 25 g fresh yeast or Libby Riley’s ginger beer and Canada, Method Starter ‘plant’ 15 g dried yeast Put the yeast into a large clean jar. Pour in 275 ml warm water. Stir in 10 ml (2 tsps) sugar and 10 ml (2 tsps) ground ginger. Cover and leave in a warm place for 24 hours. This is the starter ‘plant’. 1 kg sugar Feeding the ‘plant’ 40 ml (8 tsps) ground ginger 1. On each of the following 6 days, feed the ‘plant’ with 5 ml (1 tsp) sugar and 5 ml (1 tsp) ground ginger. Juice of 2 lemons Stir and cover the jar each time. Water 2. After the last addition, leave the solution to stand covered for another 24 hours. This recipe makes 5 litres 3. Line a sieve with muslin. Strain the solution, reserving both the liquid and the sediment. 4. Over a low heat, dissolve 900 g sugar in 575 ml water. Stir well. 5. When the sugar has dissolved, bring to the boil and boil for 3 minutes. 6. Pour the syrup into a large bowl and stir in the lemon juice and liquid from the ‘plant’. 7. Dilute with 3.5 litres water, stir well and pour into clean, rinsed out bottles. Secure the bottles with corks (do not use screw tops as yeast produces carbon dioxide as it ferments the liquid which may cause the bottles to explode). 8. Store for at least 1 week before using. Use half of the reserved sediment as the new ‘plant’ to start the next batch of ginger beer. Give the other half to a friend so they can make their own. 20 21 YOGHURT - a thick and creamy fermented dessert Josiane Dunn’s homemade yoghurt Method Yoghurt is a fermented milk product in which milk is inoculated with a starter culture containing two 1. Heat the UHT milk to 43 °C. The different types of lactic acid bacteria. The process of lactic acid bacteria grow best at yoghurt making has been around thousands of years this temperature. Take care not to and originated in the warm climate of Mesopotamia, overheat or the bacteria will be the present day Middle Eastern country of Iraq, when killed. stored goat’s and sheep’s milk fermented naturally in the high summer temperatures. The mixture was then 2. Add the starter culture and dried hung in animal skins to cool. A soft curd called ‘jugurt’ milk powder. Mix well. was formed. This is where the word yoghurt comes 3. Pour the mixture in to a pre- from. warmed wide necked vacuum flask. Replace the lid and leave for 6-8 Today a starter culture of Streptococcus thermophilus hours. and Lactobacillus bulgaricus is normally used. As the bacteria grow they use the milk sugar lactose as 4. Pour the yoghurt into a bowl and an energy source and produce lactic acid. Initially, cover with cling-film and place Streptococcus thermophilus ferments the lactose; as in a refrigerator for 4 hours. The the level of acid accumulates, its growth is suppressed. yoghurt will get even thicker Lactobacillus bulgaricus, which is more acid- tolerant, during this time. The yoghurt will continues to ferment the remaining lactose. During then be ready to eat. this process the pH drops from 6.5 to around 4.5. This inhibits the growth of spoilage microbes. All containers and utensils must be thoroughly cleaned before they Consequently, yoghurt keeps well in the fridge for are used to prevent contamination. some days. The presence of lactic acid causes the Once the yoghurt has been made it structure of the milk protein to change; this gives must be stored at 4 °C and eaten yoghurt its special thickened texture. The lactic acid with in 4 -5 days. also gives the yoghurt its sharp taste. Other products of the lactic acid fermentation, such as acetaldehyde, give the yoghurt its characteristic smell. The yoghurt can be flavoured with fruit puree as preferred. 22 23 Ingredients 575 ml UHT milk (the use of UHT milk removes the need to sterilize the milk by boiling it) 2 tbsps unpasteurized natural yoghurt or bio-yoghurt 2 tbsps dried milk powder A MICROBIAL MENU QUORN® AND MUSHROOM STROGANOFF Serves 4 Preparation time – 10 minutes Cooking time – 25 minutes Method Ingredients 1. Slice the red onion into thin 2 tbsps oil slices, crush the garlic and roughly 1 large red onion chop the mushrooms – brown cap mushrooms would be a good 2 cloves of garlic choice. 350 g QUORN® pieces 2. Heat the oil in a frying pan, add 225 g MUSHROOMS the onion, and garlic and cook 250 ml vegetable stock over a medium heat for 5 minutes. 2 tsps smoked paprika 3. Add the Quorn® pieces, mushrooms and paprika and cook for 2 dsrtsps BRANDY (optional) a further 5 minutes. 100 ml half fat CRÈME FRAICHE 4. Add the stock, brandy (optional), Seasoning to taste and cook for a further 10 minutes Chopped fresh parsley and a or until the liquid is reduced by sprinkle of paprika for garnish half. The ingredients in bold are microbial 5. Stir in the crème fraiche and seasoning to taste and cook over a medium heat for 5 minutes. 6. Garnish with chopped parsley and a sprinkle of paprika. 26 27 THAI TOFU COCONUT CURRY Ingredients 4 blocks of firm TOFU 2 bags of shredded coconut 2 tbsps each of DARK & LIGHT SOY SAUCE 4 tbsps FISH SAUCE 4 tins of coconut milk 4 onions 1 bulb of garlic 2 bags of carrots, peas and courgettes Method 4 red peppers 1. Peel 2 onions and all the garlic, de-seed 4 red peppers and 3 red chillis (leave the seeds in the other 3), peel the ginger and 6 red chillis crush the lemon grass. Place into a food processor and blend 1 bunch of lemon grass to a paste. 1 thumb of ginger 2. Peel and slice the remaining onions and set aside for later. 1 pack of kaffir lime leaves 100 ml olive oil 3. Warm a large, flat-bottomed pan and add 100 ml olive oil. When the oil is warm, add the paste from step 1, stirring Sweet chilli sauce constantly so that it doesn’t burn. When the paste becomes The ingredients in bold are microbial. less watery, add the sliced onions from step 2. 4. When the onions have taken on some colour, add the coconut milk, 1 bag of shredded coconut and 3 kaffir lime leaves. Mix Serves 3–4 Recipe supplied by Wayne Sullivan, Montpellier Chapter Hotel, Cheltenham thoroughly and bring to the boil. Reduce to a simmer and Preparation time – 15 minutes Cooking time – 45 minutes allow the sauce to reduce by roughly half the volume (approx. 30 minutes). 5. Roughly dice (1–2 cm) the vegetables and the remaining peppers. Do the same with the tofu. 6. When the curry sauce has reduced by half, check the consistency. If it appears to be too thick, add some more shredded coconut. Then add the diced vegetables, peppers and tofu and allow to cook for a further 10 minutes. 7. Remove from the heat and add 2 tbsps each of dark and light soy sauce, and 4 tbsps fish sauce. Sweet chilli sauce can be added to taste. Season with a little salt, but not too much as the soy and fish sauce are quite salty, and serve. 28 29 ACKNOWLEDGEMENTS IMAGE CREDITS The Society for General Microbiology is the Covers café menu iStockphoto (adapted I. Atherton) largest microbiology society in Europe, and has 2–3 soy sauce iStockphoto | shiitake mushrooms iStockphoto | marmite Hemera | ice cream Valueline 4–5 yoghurt iStockphoto | sauerkraut Hemera | chorizo iStockphoto | pickles Photos.com | olives Photodisc 6–7 blue cheese Polka Dot | cheese rounds Antonia Reeve SPL | brie Photos.com | over 5,000 members worldwide. An important function of the Society is the promotion of the public understanding of microbiology. The Society is cheese selection Digital Vision committed to raising awareness of microbiology and the role of microbiologists to key audiences including 8–9 schools, parliamentarians, the media and the general bread selection Stockbyte | bread in basket Photos.com | beer glass iStockphoto | beer bubbles iStockphoto | fungal growth on grapes Dr Jeremy Burgess SPL | white wine iStockphoto public. 10–11 melted chocolate Hemera | coffee beans Valueline | cocoa pods and beans iStockphoto | coffee cup iStockphoto SGM is grateful to Dr Anthony Hilton, Aston University, for his invaluable help and suggestions in 12–13 white truffles Vision SPL | Quorn® Gustoimages SPL | orange slices Hemera producing this resource. 14–15 cheese, grapes and wine Photos.com 16–17 bagels Hemera | stacked bagels Creatas 18–19 elderflower cordial iStockphoto | elderflowers Hemera 20–21 ginger root iStockphoto | ginger beer iStockphoto 22–23 yoghurt in dish iStockphoto | jar of yoghurt iStockphoto We also thank Wayne Sullivan, Montpellier Chapter 24–25 set table Hemera Hotel, for providing the Thai tofu coconut curry recipe. 26–27 mushrooms Goodshoot | garlic, parsley and red onion iStockphoto | paprika Hemera 28–29 lemon grass and chillis Hemera | tofu pieces iStockphoto | coconut Digital Vision | Written byDariel Burdass SGM lime leaves Hemera Proofread by Laura Udakis SGM 30–31 cocoa beans and chocolate iStockphoto Design & production Ian Atherton SGM Printed by Making Impressions, Yateley, Hampshire Every care has been taken to ensure that the information provided this resource is correct, but the author will be pleased to learn of any errors that have remained undetected. © 2011 Society for General Microbiology Microbiology society for general 30 31 All provided through Thinkstock except those marked SPL (Science Photo Library) Microbiology w w w. s g m . a c . u k society for general Marlborough House, Basingstoke Road Spencers Wood, Reading RG7 1AG, UK t 0118 988 1802; e [email protected]
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