ISOLATION AND SCREENING OF PURPLE-NONSULFUR PHOTOSYNTHETIC BACTERIA FOR POLYβ-HYDROXYBUTYRATE PRODUCTION
ผูรับผิดชอบโครงการ
เผยแพรผลงาน
: Chortip Lorrungruang, Prapon Sarakot, Pongsak Ackarabunpojoue,
Songkra Chuaekrut, Napavarn Nopparatnaraporn
: The 5th Asia-Pacific Biochemical Engineering Conference 1999 and The 11th
Annual Meeting of the Thai Society for Biotechnology 15- 18 November, Phuket
A number of twenty-five strains of purple-nonsulfur photosynthetic bacteria were isolated from soil,
mud and water in the natural evironments. These isolated strains and twenty-eight strains from department of
Microbiology, Kasetsart University were screened for poly-β-hydroxybutyrate (PHB) production. Three
isolates, M15m, 5B and FFW accumulated high amount of PHB with 123.27, 110.56 and 108.28 µg/ml cell
dry weight and attained high cell mass with 1.10, 0.72 and 1.44 mg/ml respectively under illuminate-anaerobic
condition in modified GM broth at 37°C for two-day culture time. The effect of illuminate-anaerobic and
unilluminate-aerobic culture condition on PHB production of M15m, 5B and FFW were also studied. It was
found that all strains were able to accumulate PHB with high yields and hydrogen gas was also produced under
illuminate-anaerobic culture condition moreover, these strains produced exopolysaccharide under unilluminateaerobic culture condition.