Gene
Enzyme function
ddlA
D-Ala-D-Ala ligase
leuS
Leucyl tRNA synthetase
ilvE
Branched-chain aminotransferase
pyrE
Orotate phosphoribosyltransferase
dapE
N-succinyl-L-L-diaminopimelic acid
desuccinylase
pyc
Pyruvate carboxylase
glpQ
Glycerophosphodiester
phosphodiesterase
Primer
ddl-f
ddl-r
leus-f
leus-r
Sequence (5'-3')
GTTTTCCCAGTCACGACGTTGTTAGAATCGCTGGACCGTGA
TTGTGAGCGGATAACAATTTCGCCCATTCTTGAAACTAATTTGATCG
GTTTTCCCAGTCACGACGTTGTAGCTATTGCTTGGAGCGAAA
ilve-f
ilve-r
pyre-f
pyre-r
dape-f
dape-r
GTTTTCCCAGTCACGACGTTGTATTGCATTATGGGCAGACG
TTGTGAGCGGATAACAATTTCACTTTGTAATGCTCGGCAAA
GTTTTCCCAGTCACGACGTTGTACCTTTCACATGGGCTAGTG
TTGTGAGCGGATAACAATTTCCTCCGCTTTAATTGAACCCATTTC
GTTTTCCCAGTCACGACGTTGTCGGAGAAGAAACTTCTGCAA
pyc-f
pyc-r
glpq-f
glpq-r
GTTTTCCCAGTCACGACGTTGTCAGATTTTAGGCGATACTCATG
TTGTGAGCGGATAACAATTTCCTCTGGAGATGTATCAATAAAAGTTGTTT
GTTTTCCCAGTCACGACGTTGTAATAATTGCCCATCGAGGAA
TTGTGAGCGGATAACAATTTCTCATTAATGCTATCTCTGGATAATCTG
TTGTGAGCGGATAACAATTTCCTTCAACTTCAGATTCAACTAGGAAA
TTGTGAGCGGATAACAATTTCCTCAATAGATTGTAAATAGGCCTCAA