Coral Bleaching
Group: 1
Presentation 2
By: Sammiejo Fat
What’s Happening to the Zoox??
• Last week our Zoox count
decreased.
• Not only our we stressing the
Aiptasia with one main
stressor but they are not
getting nutrients they need.
• Remember to take this in to
account.
Finding the Chlorophyll Content
Wavelength Readings:
At 665nm-.013nm
• Place numbers in
equation given to find
chlorophyll-a in
micrograms per.
milliliter
At 647nm-.013nm
Results: 0.13059
At 630nm-.043nm
Approximating the Biomass of Our
Aiptasia
Absorbancy (nm)
Protein Standard Curve
A = 1.2146x - 0.0492
R2 = 0.9534
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
Series1
• The Biomass of an
Aiptasia contains
protein
Linear (Series1)
0
0.5
1
• How much protein?
1.5
BSA Protein
Protein
Trial 1
Trial 2
Average
0.25
0.245
0.259
0.252
0.5
0.459
0.459
0.459
0.75
0.972
0.941
0.9565
1
1.354
1.213
1.2835
1.25
1.317
1.399
1.358
• We used the Bradford
(Coomassie Blue)
Assay to find out.
Heat Waves
• A heat wave is a prolonged period of excessively
hot weather, which may be accompanied with
high humidity.
• There is no universal definition of a heat wave;
the term is relative to the usual weather in the
area.
• The term is applied both to routine weather
variations and to extraordinary spells of heat
which may occur only once a century.
– The most recent and devastating heat wave would
have to be in 1998 after El Nino
A Picture of Average Ocean
Temperature
Our Group Experiment
• Last week we rose the temperature 10* C
– Using a small heater
• And on Friday I came and removed the
heater after observing the Aiptasia
– They had turned a very gray color and had
become white on the bottom
• We wanted to see the recovery of the
Aiptasia, after a heat wave to relate it the
coral in the oceans dealing with the
extreme changes in their environment
Sources
• http://en.wikipedia.org/wiki/Heat_wave
• http://www.worldviewofglobalwarming.org/
pages/references7.html
• http://www.noaa.gov/