3M™ Attest™ Rapid 5 Steam-Plus Test Pack 41382
Competency Assessment for use of the
3M™ Attest™ Rapid 5 Steam-Plus Test Pack 41382
in conjunction with the 3M™ Attest™ Auto-reader 290 or 390
Employee Name: ________________________________ Date: ______________________
Facility: ________________________________________ Dept: ______________________
Assessor’s Name: ________________________________ Employee Competent? Yes No
Observation Checklist
Task
Demonstrates
Competency
The 41382 test pack is placed flat, with label side up, on the bottom
shelf, over the drain, in full sterilizer
Test pack is run in an appropriate cycle [250°F (121°C) gravity ≥30
minutes or 270°F (132°C) pre-vacuum ≥4 minutes steam sterilization
cycle]
41382 test pack is retrieved from sterilizer and external process
indicator is checked to verify it has turned brown or darker
41382 test pack is opened, coil is slipped off 1292 BI and BI is
allowed to cool outside test pack for 10 minutes
Comply™ SteriGage™ Steam Chemical Integrator is checked to
verify the dark color has reached the ACCEPT window. Integrator
result is documented according to facility policy
Safety glasses are donned
The process indicator on the 1292 BI label is checked to confirm it
has changed from rose to brown
1292 BI is identified (i.e., load #, sterilizer #, and date are written on
BI label)
1292 BI is activated following these steps: BI cap is closed, BI is
placed in Auto-reader crusher well and media ampoule is crushed,
BI is tapped on hard surface to distribute growth media
BI is placed in incubation well that is color-coded brown in Attest™
Auto-reader Model 290 or Model 390
User verifies Auto-reader has recognized placement of BI in
incubation well (as indicated by yellow light on Model 290 or 180
minute countdown time on Model 390)
Test BI result, as indicated on Auto-reader, is correctly interpreted
Test BI lot # and result are documented according to facility policy
Control BI lot # and result are documented or user verifies a control
BI (having the same lot # as the test BI) result has already been
documented for the day
(Please turn over for Written Assessment component of competency)
Written Assessment
1. Biological indicators (BIs) are used to assess the lethality of a sterilization cycle.
True
False
2. For steam sterilizers, our facility’s policy is to run a BI process challenge device (BI PCD),
such as the Attest™ 41382 Rapid 5 Test Pack,:
a) In every load
b) Daily and in every implant load
c) Weekly and in every implant load
d) Other
3. According to AAMI ST79, BIs used to monitor steam sterilizers should contain spores of which
microorganism?
a) Bacillus atrophaeus
b) Geobacillus stearothermophilus
c) Clostridium difficile
4. A control 1292 BI, having the same lot # as the test packs, should be placed in each Auto-
reader at what frequency?
a. Daily and whenever a box having a new lot number is opened
b. Weekly
c. Once/box of biological indicators or test packs
5. According to AAMI ST79, implant loads should be monitored with a BI PCD that also contains
a Class 5 integrating indicator. In the event of a documented medical emergency, the results
of the Class 5 integrating indicator may be used as a basis for early load release of an implant.
a. True
b. False
6.
After a successful sterilization cycle, the Attest™ 1292 BI requires ____ of incubation before a
negative result (indicated by a green light under the incubating BI in an Auto-reader 290 or a
‘-‘ symbol under the incubating BI on the Auto-reader 390 LCD display) is displayed:
a. 30 minutes
b. 1 hour
c. 3 hours
d. 48 hours
7. A positive result (red light on the Auto-reader 290 or ‘+‘ symbol on the Auto-reader 390 LCD
display) for a 1292 BI from a processed 41382 test pack indicates:
a. a failed sterilization cycle
b. a successful sterilization cycle
8. When a positive result occurs for a processed BI, and the cause of the failure cannot be
immediately identified, (circle correct answer)
a. No action is necessary
b. That load should be quarantined.
c. The sterilizer should be removed from service, all items processed in the sterilizer since the
last cycle having a negative BI result should be recalled, and the supervisor should be notified.
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