HOW TO PREPARE DIFFERENT CONCENTRATIONS OF GLUCOSE BAGS DESIRED FINAL CONCENTRATION 10% 12.5% 15% 17.5% 20% 25% 30% 35% 40% 50% AMOUNT OF 10% GLUCOSE REQUIRED AMOUNT OF 20% GLUCOSE REQUIRED AMOUNT OF 50% GLUCOSE REQUIRED TOTAL FINAL VOLUME PRE-PREPARED BAGS ARE COMMERCIALLY AVAILABLE AT THIS CONCENTRATION 487.5ml N/A 32.5ml 520ml 437.5ml N/A 62.5ml 500ml 422.5ml N/A 97.5ml 520ml PRE-PREPARED BAGS ARE COMMERCIALLY AVAILABLE AT THIS CONCENTRATION N/A 425ml 85ml 510ml N/A 340ml 170ml 510ml N/A 250ml 250ml 500ml N/A 170ml 340ml 510ml PRE-PREPARED VIALS ARE COMMERCIALLY AVAILABLE AT THIS CONCENTRATION HOW TO PREPARE DIFFERENT CONCENTRATIONS OF GLUCOSE BAGS Calculation for making different glucose concentrations: 1. Use a grid like the one below and write the two concentrations of the glucose you are mixing in the column on the left. The highest available concentration should be in cell A in the top left of the grid 2. Put the desired final concentration of glucose in the column in the middle 3. Write the difference between the number in cell A and the number in the middle column in cell D (follow the arrow) 4. Write the difference between the number in cell B and the number in the middle column in cell C (follow the arrow) Concentration of glucose solutions to be mixed to make the desired final concentration of glucose (put highest value at top left) Desired final concentration 50% Number of parts (this is the difference between the number in the left column and the middle column – follow the arrows) 2.5 A C 12.5% 10% B 37.5 D 5. Calculate the volume of solution A required by dividing the number in cell C by the total number of parts (C+D) and multiply this by the total volume of your final solution (you can choose this volume but it should not exceed 550mls as you will not be able to fit this in the bag of fluid) In the example above = 2.5/40 x 520ml = 32.5mls of 50% glucose required (50% glucose = 50grams in 100ml = 0.5g in 1ml so there are 16.25grams in 32.5ml of 50% glucose) 6. Calculate the volume of solution B required by dividing the number in cell D by the total number of parts (C+D) and multiply this by the total volume of your final solution In the example above = 37.5/40 x 520ml = 487.5mls of 10% glucose required (10% glucose = 10g in 100ml = 0.1mg in 1ml so there are 48.75grams in 487.5ml) 7. Final check = the final mixed solution will have 48.75grams + 16.25 grams = 65grams in 520ml = 12.5grams in 100ml = 12.5% glucose
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