Research Paper
Mating type genes and genetic markers to decipher intra-specific variability
among Fusarium udum isolates from pigeonpea
Prem Lal Kashyap1, Shalini Rai1, Sudheer Kumar1, Alok Kumar Srivastava1, M.
Anandaraj2 and Arun Kumar Sharma1
1
ICAR-National Bureau of Agriculturally Important Microorganisms, Mau, Uttar Pradesh, India
2
ICAR-Indian Institute of Spices Research (IISR), Marikunnu, Calicut, Kerala, India
DOI 10.1002/jobm.201400483
Table S1. List of RAPD primers used in the present study.
Primer
Sequence
OPA1
CAGGCCCTTC
OPA2
TGCCGAGCTG
OPA3
AGTCAGCCAC
OPA4
AATCGGGCTG
OPA5
AGGGGTCTTG
OPA6
GGTCCCTGAC
OPA7
GAAACGGGTG
OPA8
GTGACGTAGG
OPA9
GGGTAACGCC
OPA10
GTGATCGCAG
OPA11
CAATCGCCGT
OPA12
TCGGCGATAG
OPA13
CAGCACCCAC
OPA14
TCTGTGCTGG
OPA15
TTCCGAACCC
OPA16
AGCCAGCGAA
OPA17
GACCGCTTGT
OPA18
AGGTGACCGT
OPA19
CAAACGTCGG
OPA20
GTTGCGATCC
Table S2. Comparison of different genetic markers to decipher intra-specific variability among
F. udum isolates from pigeonpea.
Total number of bands (Amplicon size in bp)
Isolate
(s)
Fu1
Fu2
Fu3
Fu4
Fu5
Fu6
Fu7
Fu8
Fu9
Fu10
RAPD
14
(450, 530, 700, 780, 800,
900, 960, 1000,1150, 1300,
1350, 1400, 1780, 2500)
15
(400, 530, 600, 700, 780,
900, 1000, 1150,1300, 1350,
1400, 1500, 1650, 1780,
2000)
17
(400, 450, 530, 700, 780,
800, 900, 960, 1000,
1150,1300, 1350, 1400,
1500,1650, 1780, 2000)
19
(400, 450, 530, 590, 600,
700, 780, 800, 900, 960,
1000,1150, 1300,1350,
1400, 1500, 1650, 1780,
2000)
9
(400, 480, 600, 1000, 1350,
1400, 1500, 1700, 1780)
10
(400, 450, 480, 800, 960,
1000, 1300, 1350, 1500,
1780)
12
(450, 480, 600, 700, 780,
800, 960, 1000, 1300, 1350,
1500, 1780)
4
(480, 1180, 1350, 1500)
16
(400, 500, 580, 590, 650,
700, 750, 850, 1000,1180,
1300, 1350, 1400, 1500,
1650, 2000)
13
(400, 700, 780, 850, 900,
1000, 1180, 1250,
1300,1400, 1500, 1650,
2000)
ERIC
9
(100, 150, 200, 300, 580,
600, 800, 850, 1000)
9
(100, 150, 200, 300, 580,
600, 800, 850, 1000)
10
(100, 150, 200, 300, 580,
600, 800, 850, 1000,1350)
9
(100, 150, 200, 300, 580,
600, 800, 850, 1000)
9
(100, 150, 200, 300, 580,
600, 800, 850,1000)
9
(100, 150, 200, 300, 580,
600, 800, 850,1000)
BOX
14
(350, 400, 500, 680, 700,
760, 800, 900, 1000,
1290, 1500, 2300, 2500,
3500)
15
(350, 400, 500, 680, 700,
760, 800, 900, 1000,
1180, 1290, 1500, 2300,
2500, 3500)
15
(350, 400, 500, 680, 700,
760, 800, 900, 1000,
1180, 1290, 1500, 2300,
2500, 3500)
16
(350, 400, 500, 680, 700,
760, 800, 900, 1000,
1180, 1290, 1500, 2300,
2500, 3000, 3500)
14
(350, 500, 680, 700, 760,
800, 900, 1000, 1180,
1290, 1500, 2300, 2500,
3500)
7
(100, 300, 700,
800,850,1000, 3200)
4
(700, 1500, 2500, 3500)
13
(350, 600, 680, 700, 760,
800, 1000, 1180, 1290,
1500, 2300, 2500, 3500)
13
(180, 200, 350, 400, 500,
600, 800, 900, 1290,
1500, 2300, 2500, 3000)
16
(180, 300, 350, 400, 500,
600, 680, 700, 760, 900,
1000, 1290, 1500, 1890,
2500, 4000)
9 (100, 200, 500, 580,
680, 700, 800,850,1000)
11
(350, 400, 500, 600, 700,
760, 800, 900, 1000,
1180, 1290)
10
(100, 150, 200, 300, 580,
600, 800, 850,1000, 1180)
8
(100, 300, 580, 700, 800,
850,1000, 3200)
Microsatellite
3
(200,300,600)
3
(200,300,600)
3
(200,300,600)
3
(200,300,600)
3
(200,300,600)
3
(200,300,600)
3
(200,300,600)
3
(200,300,500)
3
(200,300,500)
3
(200,300,600)
Fu12
13
(300, 400, 480, 700, 780,
800, 1000, 1250, 1350,
1400, 1500, 1780, 2000)
13
(400, 480, 580, 590, 600,
700, 850, 900, 1000,
1150,1350, 1500, 2000)
3
(700, 800,850)
13
(200, 260, 300, 500, 580,
600, 680, 700, 800,900,
1500, 2500, 3200)
Fu13
12
(400, 480, 590, 600, 650,
700, 750, 780, 850, 1000,
1250, 2500)
3
(580, 680, 800)
Fu11
Fu15
9
(530, 600, 650, 700, 780,
850, 900, 1250, 2000)
11
(400, 600, 780, 850, 900,
1000, 1150, 1300,
1400,1500, 2000)
Fu16
14
(400, 480, 590, 600, 650,
700, 780, 850, 900, 1000,
1250, 1300, 1400, 2000)
Fu14
Fu17
Fu18
Fu19
Fu20
10
(530, 650, 700, 780, 850,
900, 1250, 1300, 1400,
2500)
15
(400, 550, 600, 650, 700,
780, 850, 900, 1000, 1150,
1250,1300, 1350, 1400,
1500)
17
(400, 480, 530, 550, 650,
700, 780, 850, 1000, 1150,
1250, 1300, 1350, 1400,
1500, 1650, 2000)
15
(400, 480, 530, 600, 650,
700, 780, 850, 1150, 1300,
1350, 1400, 1650, 2000,
2500)
4
(500, 600, 760, 800)
7
(350, 500, 600, 760, 800,
900, 2500)
15
(350, 500, 600, 760, 800,
900, 1000, 1180, 1290,
1500, 1890, 2300, 2500,
3000, 3500)
16
(350, 500, 700, 760, 800,
900, 1000,1180, 1290,
1400, 1890, 2300, 2500,
3000, 3500, 4000)
13
(350, 700, 760, 800, 900,
1000, 1290, 1500, 1890,
2300, 2500, 3500, 4000)
17
(200, 300, 350, 400, 500,
600, 700, 760, 800,1000,
1290, 1400, 1890, 2300,
2500, 3500, 4000)
3
(300,300,600)
3
(300,300,600)
3
(200,300,500)
8
(100, 300, 700, 800,900,
1500,2000, 3200)
13
(200, 260, 300, 500, 580,
600, 700, 800,900, 1000,
1350, 1500, 3200)
15
(100, 200, 260, 300, 350,
400, 500, 580, 700,
800,900, 1000, 1350,
1500, 3200)
15
(100, 200, 260, 300, 350,
400, 500, 580, 700,
800,900, 1000, 1350,
1500, 3200)
13
(300, 400, 500, 600, 680,
700, 760, 800, 900, 1000,
1180, 1290, 4000)
3
(200,300,500)
11
(100, 200, 300, 580, 700,
800,900, 1000, 1350,
1500, 3200)
12
(200, 400, 500, 600, 680,
700, 800, 900, 1290,
1500, 1890, 2300)
3
(200,300,500)
10
(100, 200, 300, 580, 700,
800,900, 1000, 1350,
3200)
7
(200, 400, 500, 680, 700,
800, 900)
3
(200,300,500)
10
(100, 200, 300, 580, 750,
800, 900, 1000,
1350,3200)
11
(400, 500, 680, 700, 800,
900, 1290, 1500, 1890,
2300, 3500)
3
(200,300,500)
3
(200,300,500)
3
(200,300,500)
3
(200,300,500)
Figure S1. Morphological (A) and microscopic (B) features of representative Fu isolates.
Figure S2. Combined dendrogram showing genetic relationship among 20 Fu isolates based on
microsatellites, RAPD, ERIC- and BOX-PCR. Scale coefficient of similarity.