Nicholas Lawson 417-850-2073 [email protected] 611 S. Silver St. Apt. 2 Centralia, WA 98531 Profile I am an energetic highly motivated scientist with a passion for conducting research and data analysis. My key strengths are critical thinking and attention to detail. While conducting my MS thesis research, I became proficient in a number of skills including experimental design, nucleic acid isolation along with many downstream processes (e.g., sequencing library prep, PCR, and qPCR). I have experience working extracting DNA and RNA from many genus of fungal plant pathogens as well as rice and soybeans. Experience Laboratory Manager - Landrace Labs, Rochester, WA April 2016 – Current Landrace is a supercritical CO2 plant oil extraction company. As the lab manager, I’ve helped this start-up tremendously first I fixed an assembly error in the extraction machine. I am in charge of updating and writing SOPs, handling lab intake, forecasting production and take care of all routine maintenance of the laboratory equipment. Graduate Research Assistant Fungal Genetic Laboratory, PI Dr. Burt Bluhm, University of Arkansas June 2014 - Dec 2015 Research focused on soybean pathogen Cercospora sojina. I became proficient in: sterile technique fungal isolation, fungal genetic transformation, molecular cloning, maintaining bacterial cultures, end point PCR, gel extraction of nucleic acids, and southern blotting. I also extracted RNA, built cDNA libraries and helped run an Ion-Torrent PGM sequencer at the University of Arkansas. Graduate Research Assistant Molecular Plant Biology, PI Dr. Ken Korth, University of Arkansas Aug 2011 - May 2014 The in order to complete the objectives of my thesis we utilized RNA-seq, performed at UC Davis sequencing center, AffyMatrix Gene Chip Microarrays, performed at MSU, and qPCR to compare gene expression between two rice cultivars. I carried out the entire rice growth experiments from planting the rice to collecting RNA from endosperm. All downstream data analysis and follow up qPCR verification was performed by myself. Education Master of Science, University of Arkansas, Fayetteville, AR May 2016 Concentration: Cell and Molecular Biology Thesis Title: “Gene Expression and Physiological Analysis to Study Differences Between Oryza sativa Cultivars Susceptible and Resistant to Chalky Grain Formation Subjected to High Nighttime Temperatures.” Bachelor of Science, Missouri Southern State University, Joplin, MO May 2010 Concentration: Biology & Genetics Service/Leadership President, Plant Pathology Graduate Student Association Member, Plant Pathology Graduate Student Association Founding Member, The Student Mycology Club 2015 2012 - 2016 2014 - 2016 Skills Laboratory: Nucleic acid isolation, NGS library prep, NCBI BLAST, primer design, PCR, qPCR, confocal microscopy, ELSA, molecular cloning Advanced bioinformatics skills: Max OSX, Windows, Unix, perl script, and installation and utilization of open source analysis toolkits Research, design, manage, and perform complex research projects Statistical analysis software: JMP, SAS, R Lab management: Managing hourly employees, lab supply ordering, both equipment and consumables
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